Trang 1 TRƯỜNG ĐẠI HỌC NÔNG LÂM THÀNH PHỐ HỒ CHÍ MINHKHOA KHOA HỌC SINH HỌCBÁO CÁO MÔN HỌC MÔN TIN SINH HỌCNgành học: CÔNG NGHỆ SINH HỌCNhóm thực hiện: Nhóm 19Niên khóa : 2021 - 2025 Tr
LẤY SEQUENCE VÀ XÁC ĐỊNH TÊN LOÀI TỪ CÁC ACCESSION NUMBER
Qui trình thực hiện
Bước 1: Truy cập vào trang web NCBI
Bước 2: Tiếp đó tại thanh tìm kiếm ta nhập các accession mà ta cần tìm Bên trái thanh tìm kiếm ta chuyển từ chế độ tìm “All Databases” sang chế độ “Nucleotide” Sau đó chọn Enter
Hình 2.2 Nhập accession vào thanh tìm kiếm.
Bước 3: Sau đó tại cửa sổ trang mới ta chọn “FASTA” để lấy các tình tự accession.
Kết quả
Mẫu KP699121.1 thuộc loài Bacillus subtilis Trình tự của mẫu là:
>KP699121.1 Bacillus subtilis strain GnzIII 16S ribosomal RNA gene, partial sequence
CTATACATGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTTGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAGGAA
Mẫu HF562894.1 thuộc loài Bacillus tequilensis.
>HF562894.1 Bacillus tequilensis partial 16S rRNA gene, isolate S42 TGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGA CGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGG GAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACAT AAAAGGTGGCTTTGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCT AGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAG AGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGA GGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACG CCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAG AACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAA GCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGC GTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCT GATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAA CTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCG TAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAAC TGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGG TAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCT TAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCA AGACTGAAACTCAAAGGAATTGACGGGGCCCGCACAGCGTGGAGCATGTG TTTATTCGAGCACGCGAGACTTACAGGTCT
Mẫu KT273321.1 thuộc loài Bacillus pumilus.
>KT273321.1 Bacillus pumilus strain AZ-1 16S ribosomal RNA gene, partial sequence
GGGAGCTTGCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGCATGGTTCAAGGATGAAAGACGGTTTCGGCTGTCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCGAGAGTAACTGC
TCGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAG CAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGT AAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCA ACCGGGGAGGGTCATTGGAAACTGGGAAACTTGAGTGCAGAAGAGGAGA GTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACC AGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAG CGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGA TGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCA TTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAA TTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAA CGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAAATAG GGCTTTCCCTTCGGGGACAGAGTGACAGGTGGTGCATGGTTGTCCTCAGCT CGGGCCGGGAAATGTTGGGTTAAGTCCCGCAACGAACGCAACCTTTGATC TTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAAC CCGGAGGAAGGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTG GGCTACACACGTGCTACAATGGACAGAACAAAGGGCTGCAAGACCGCAAG GTTTAGCCAATCCCATAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACT CGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGG TGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTG CAACACCCGAAGTCG
Mẫu KT216619.1 thuộc loài Bacillus vallismortis
>KT216619.1 Bacillus vallismortis strain DD007 16S ribosomal RNA gene, partial sequence
TGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACC
TAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTA GGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGT TTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGA AACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCG GTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCT GGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTA GATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGG TTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAG TACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGC GGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTC TTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAG TGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTA AGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGG CACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGT CAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACA GAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTT CTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTA GTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACAC ACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAA CCTTTTAGGAGCCAGCCGCCGAAGGTGGGACA
Mẫu KR709243.1 thuộc loài Bacillus cereus
>KR709243.1 Bacillus cereus strain UBT4 16S ribosomal RNA gene, partial sequenceCGGCCAGTAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGAAGGGAGCTTGCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGCATGGTTCAAGGATGAAAGACGGTTTCGGCTGTCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCGAGAGTAACTGCTCGCACCTTGACGGTACCTAACC
AGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGG CAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTA AGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTG GGAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAA ATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCT GTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATA CCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCC GCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGG TCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGG AGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGAC ATCCTCTGACAACCCTAGAGATAGGGCTTTCCCTTCGGGGACAGAGTGACA GGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCC CGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTC TAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAT CATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACA AAGGGCTGCAAGACCGCAAGGTTTAGCCAATCCCATAAATCTGTTCTCAGT TCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATC GCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCC CGTCACACCACGAGAGTTTGCAACACCCGAAGTCGGTGAGGTAACCTTTAT GGAGCCAGCCGCCGAAGGTGGGGCAGATGATTGGGGTGAAGTCGTAACAA GGTATCCGGTCATATCTGTTTCCTG
Mẫu KP940383.1 thuộc loài Bacillus safensis
>KP940383.1 Bacillus safensis strain CCH3X 16S ribosomal RNA gene, partial sequence
CGCGGGCTATACATGCAAGTCGAGCGGACAGAAGGGAGCTTGCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGCATGGTTCAAGGATGAAAGACGGTTTCGGCTGTCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTC
TGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTC TGTTGTTAGGGAAGAACAAGTGCGAGAGTAACTGCTCGCACCTTGACGGT ACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATAC GTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGC GGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATT GGAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTA GCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACT CTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGG ATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGG GGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGG GAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACA AGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAG GTCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTTCCCTTCGGGGACA GAGTGACAGGTGGTGCATGATGTCGTCAGCTCGTGTCGTGAGATGTTGGGT AGTCCCGCACGAGCGCACCTTGATCTAGTGCCAGCATTCAGTGGCACTCTA GTGACTGCATGACAACCGAGAAGGGGGGGAATGAACGTCAATCATC
Mẫu KT719573.1 thuộc loài Bacillus infantis
>KT719573.1 Bacillus infantis strain MER_TA_169 16S ribosomal RNA gene, partial sequence
GTCGAGCGGACGGATGGGAGCTTGCTCCCTGAAGTCAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGGAGCTAATACCGGATAATGCACAGCCTCTCATGAGGCTATGCTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTCAGGGAAGAACAAGTGCCGGAGTAACTGCCGGCACCTTGACGGTACCTGACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGGACTTGA
GTGCAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAG ATGTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGAC GCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT CCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGT GCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGC TGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGG TTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCTCCTGAC AACCCTAGAGATAGGGCGTTCCCCTTCGGGGGACAGGATGACAGGTGGTG CATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACG AGCGCAACCCTTGATCTTAGTTGCCAGCATTAGGTTGGGCACTCTAAGGTG ACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATG CCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGCT GCAAGACCGCGAGGTTAAGCGAATCCCATAAAACCATTCTCAGTTCGGAT TGCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCGCGGA TCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCA CACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTGGAGCC AGCCGC
Mẫu HQ397586.1 thuộc loài Bacillus firmus.
>HQ397586.1 Bacillus firmus strain BSCS3 16S ribosomal RNA gene, partial sequence
ACATGCAGTCGAGCGGACGGATGGGAGCTTGCTCCCTGAAGTCAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGGAGCTAATACCGGATAATGCACAGCCTCTCATGAGGCTATGCTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTCAGGGAAGAACAAGTGCCGGAGTAACTGCCGGCACCTTGACGGTACCTGACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGGA
CTTGAGTGCAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATGCG TAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAAC TGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGG TAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTT TAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCA AGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCAT GTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCTCC TGACAACCCTAGAGATAGGGCGTTCCCCTTCGGGGGACAGGATGACAGGT GGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGC AACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAA GGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCAT CATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAG GGCTGCAAGACCGCGAGGTTAAGCGAATCCCATAAAACCATTCTCAGTTC GGATTGCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCG CGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCC GTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTGG AGCC
Mẫu KJ000207.1 thuộc loài Bacillus thuringiensis.
>KJ000207.1 Bacillus thuringiensis strain VKK-GA1 16S ribosomal RNA gene, partial sequence
CGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGGAGCTAATACCGGATAATGCACAGCCTCTCATGAGGCTATGCTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTCAGGGAAGAACAAGTGCCGGAGTAACTGCCGGCACCTTGACGGTACCTGACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATG
CGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTA ACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCT GGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCC TTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCG CAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAG CATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATC TCCTGACAACCCTAGAGATAGGGCGTTCCCCTTCGGGGGACAGGATGACA GGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCC CGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTAGGTTGGGCACTC TAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAT CATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACA AAGGGCTGCAAGACCGCGAGGTTAAGCGAATCCCATAAAACCATTCTCAG TTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAAT CGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTA
Mẫu KM041133.1 thuộc loài Bacillus drentensis.
>KM041133.1 Bacillus drentensis strain G5C_0m_04 16S ribosomal RNA gene, partial sequence
TGCAGTCGAGCGGACGGATGGGAGCTTGCTCCCTGAAGTCAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGGAGCTAATACCGGATAATGCATAACCTCTCATGAGGTTATGCTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTCAGGGAAGAACAAGTGCCGGAGTAACTGCCGGCACCTTGACGGTACCTGACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTA
GTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTA GTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAG GCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGT GGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCTCCTG ACAACCCTAGAGATAGGGCTTTCCCCTTCGGGGGACAGGATGACAGGTGG TGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAA CGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGG TGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCA TGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGG GCTGCAAGACCGCGAGGTTAAGCGAATCCCATAAAACCATTCTCAGTTCG GATTGCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCGC GGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCG TCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTGGA GCCA
Mẫu KT274776.1 thuộc loài Bacillus licheniformis.
>KT274776.1 Bacillus licheniformis strain DMB31 16S ribosomal RNA gene, partial sequence
GGATGCTTGATTGAACCGCATGGTTCAATTATAAAAGGTGGCTTTTAGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGG
AATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGC AACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGAT AGGGCTTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCA GCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGA TCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAA ACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTG GGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAGCCGCGA GGCTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAAC TCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCG GTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTT TGTAACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAAG GTGGGACAGATGATTGGGG
Mẫu KP296232.1 thuộc loài Bacillus sonorensis>KP296232.1 Bacillus sonorensis strain Na5RB-2 16S ribosomal RNA gene, partial sequence
ATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGATTGAACCGCATGGTTCAATTATAAAAGGTGGCTTTTAGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGC
AACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGAT AGGGCTTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCA GCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGA TCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAA ACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTG GGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAGCCGCGA GGCTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAAC TCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCG GTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTT TGTAACACCCGAAGTCGGTGAGGTAACCTTTG
Mẫu EU833948.1 thuộc loài Pseudomonas putida.
>EU833948.1 Pseudomonas putida strain FWC30 16S ribosomal RNA gene, partial sequence
GAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAA CCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGAT GGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTYGGCTACCACT TACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACC AAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGAC TGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGC AATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTC GGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGG CGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAG CAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGT AAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCA ACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGA GTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACC AGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGC GTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAT GAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCAT Mẫu KR010178.1 thuộc loài Bacillus amyloliquefaciens.
>KR010178.1 Bacillus amyloliquefaciens strain NSB-1 16S ribosomal RNA gene, partial sequence
GTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACA GATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTG GGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATAC CGGATGGTTGTCTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCGGCTA CCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGC TCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACT GGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCT TCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGG TTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCCGTTCAAAT AGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTG CCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGG GCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGG CTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGG AGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAAC ACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAA AGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAAC GATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACG CATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGG AATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGC AACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATA GGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAG CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGAT CTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAA CCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGG GCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAG GTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACT CGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCACATGCCGGGGG GGAAAAAAACTTTCCCCGGGCCTTTGTACACACCGGCCCGTCACACCACG AAGAGTTTGTAACACCCCGAAGTCCGGTGAGGTAACCTTTTATGGAGCCA GCCGCCCGAAGGTGGGACAGATGATTGGGGTGATAG
Mẫu KT216570.1 thuộc loài Bacillus methylotrophicus.
>KT216570.1 Bacillus methylotrophicus strain SHP2-20 16S ribosomal RNA gene, partial sequence
TCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTCTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCCGGGGGAATACCGTTCCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTTAGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGA
Mẫu LN890068.1 thuộc loài Bacillus pseudomycoides.
>LN890068.1 Bacillus pseudomycoides partial 16S rRNA gene, strain L72 TCGAGCGAACCGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGG TGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAA CCGGGGCTAATACCGGATAACATTTTGCACCGCATGGTGCGAAATTGAAA GGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTG GTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGT GATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAG CAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCG TGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACA AGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCA CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATG TGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTG AGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGA GATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGAC ACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT CCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGT GCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGC TGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGG TTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAC AACCCTAGAGATAGGGCTTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCA TGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAG CGCAACCCTTGATCTTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGAC TGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCC CCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTG CAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTG TAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATC AGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACA CCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTT
Mẫu KF419123.1 thuộc loài Bacillus megaterium.
>KF419123.1 Bacillus megaterium strain QAP17 16S ribosomal RNA gene, partial sequence
CGTGCGCGCTATAATGCAAGTCGAGCGAACTGATTAGAAGCTTGCTTCTATGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGAAGCTAATACCGGATAGGATCTTCTCCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACGAGAGTAACTGCTCGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACTCTAGAGATAGAGCGTTCCCCTTCGGGGGGACAGAGTTGACAGGTGGGTGCATGGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGGCTTAAGTTCCGCCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGCTGCAAGACCGCGAGGTCAAGCCAATCCCATAAAACCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGAGTAACCGTAAGGAGCTAGCCGCCTAAGGTGGGACAGATGATTGGGGGAAGTCGAACAAGTGCG
Mẫu KP706808.1 thuộc loài Bacillus aryabhattai >KP706808.1 Bacillus aryabhattai strain SO4H51 16S ribosomal RNA gene, partial sequence
CTTCTATGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGAAGCTAATACCGGATAGGATCTTCTCCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACGAGAGTAACTGCTCGTACCTTGACGGTACCTTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACTCTAGAGATAGAGCGTTCCCCTTCGGGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGCTGCAAGACCGCGAGGTCAAGCCAATCCCATAAAACCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAAC
BLAST VÀ XÁC ĐỊNH TÊN LOÀI TỪ MẪU 4.3
Qui trình thực hiện
Bước 1: Trên thanh tìm kiếm của google, nhập từ khoá “Chromas” để tìm và tải ứng dụng.
Hình 3.1 Tìm kiếm Chromas trên google.
Bước 2: Sau khi đã tải, ta mở ứng dụng Chromas lên
Hình 3.2 Giao diện chính của Chromas.
Bước 3: Tại giao diện màn hình chính, chọn Open rồi mở fide mẫu 4.3 lên.
Hình 3.3 Sau khi mở fide chứa mẫu 4.3.
Bước 4: Sau đó ta chọn “Export” Ở mục “ Save as type” ta chọn “Fomatted text” , chọn vị trí lưu fide rồi chọn “Save” Sau khi lưu, ta truy cập tiến hành xoá những đoạn nhiễu, ở phần đầu ta xoá 60 nucleotide, ở đầu cuối ta xoá 87 nucleotide
Hình 3.4 Lưu fide sau khi đọc trình tự.
Bước 5: Truy cập từ khoá “blast ncbi” trên goolgle để tìm kiếm hoặc truy cập vào https://blast.ncbi.nlm.nih.gov/Blast.cgi để vào công cụ blast.
Hình 3.5 Tìm kiếm công cụ Blast
Bước 6: Tại giao diện màn hình chính ta chọn “Nucleotide BLAST”
Hình 3.6 Giao diện chính của BLAST.
Bước 7: Tại mục “chọn tệp” ta chọn fide của mẫu 4.3 Đặt tên mẫu tại mục “Job Title” Tại mục “optimize for” ta chọn “Optimize forSomewhat similar sequences (blastn)” Sau khi thiết lập hoàn tất ta chọn “Blast”.
Hình 3.7 Blast đoạn trình tự mẫu 4.3.
Kết quả
Hình 3.8 Kết quả thu được sau khi Blast mẫu.
Tỷ lệ Query Cover gần như 100% và chỉ số Per Ident cao cho thấy rằng trình tự DNA mẫu của chúng ta có sự tương đồng rất cao với các trình tự tham chiếu trong cơ sở dữ liệu Giá trị E cho tất cả các trình tự tham chiếu đều là 0.0 Điều này có nghĩa là khả năng ngẫu nhiên tìm thấy một kết quả tốt hơn kết quả truy vấn là rất thấp.Vì tất cả các trình tự tham chiếu đều thuộc chi Bacillus và thiếu thông tin để xác định loài cụ thể, ta có thể kết luận rằng mẫu DNA này là Bacillus sp.
XÂY DỰNG CÂY DI TRUYỀN VÀ KẾT LUẬN LOÀI CỦA MẪU 4.3
Qui trình thực hiện
Bước 1: Trên thanh tìm kiếm của google, nhập từ khoá “mega x software” để tìm và tải ứng dụng.
Hình 4.1.Tìm kiếm MEGA trên google.
Bước 2: Sau khi tải, truy cập vào ứng dụng MEGA
Hình 4.2 Giao hiện màn hình chính của MEGA.
Bước 3: Tại giao diện màn hình chính của MEGA, ta chọn “Align” , sau đó chọn “ Edit/ Build Alignment”.
Hình 4.3 Tạo một Alignment mới.
Bước 4: Một cửa sổ nhỏ hiện ra, ta chọn “Create a new alignment”, sau đó chọn “OK”.
Tiếp tục chọn “DNA” trong bảng mới xuất hiện sau đó
Hình 4.4 Chọn thiết lập cho Alignment mới.
Bước 5: Tại giao diện cửa sổ mới ta chọn “Edit”, sau đó nhấn chọn “Insert Sequence From File” Tại đây, chọn fide Text document có chứa các sequence của trình tự mẫu 4.3 và các sequence của các accession number đã tìm ban đầu cần so sánh.
Hình 4.5 Thêm các tình tự cần so sánh tương đồng.
Bước 6: Xoá Sequence trống cuối Sau đó chọn “Alignment”, sau đó chọn “Alignment by ClustaIW”.
Hình 4.6 Các trình tự sau khi được so sánh.
Bước 7: Chọn “Data” , chọn “Export Alignment” để xuất fide, tiếp tục chọn định dạng của file là “MEGA format” Sau đó chọn “Save” để lưu thư mục
Hình 4.7 Lưu fide trình tự đã so sánh.
Step 8: In the MEGA software interface, select the icon labeled "Phylogeny", then select "Construct/Test Neighbor - Joining Tree " Choose the previously saved MEGA fide, then select "OK".
Kết quả
Hình 4.8 Cây di truyền Test Maximum Likelihood Tree.
Hình 4.9 Cây di truyền Test Neighbor-Joining Tree
Hình 4.10 Cây di truyền Test Minimum-Evolution Tree
