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SỞ KHOA HỌC CÔNG NGHỆ TP.HCM VIỆN PASTEUR TP.HCM BÁO CÁO NGHIỆM THU Đề tài nghiên cứu cấp Sở KH-CN GIÁM SÁT BIẾN ĐỔI GEN VIRÚT CÚM A/H5N1 LƯU HÀNH TẠI KHU VỰC PHÍA NAM NĂM 2006 – 2008 VÀ NGHIÊN CỨU ỨNG DỤNG QUY TRÌNH TẠO VIRÚT CÚM GIẢM ĐỘC LỰC BẰNG KỸ THUẬT DI TRUYỀN NGƯỢC TRÊN CHỦNG VIỆT NAM Cơ quan chủ quản: SỞ KH-CN TP.HCM Cơ quan chủ trì: VIỆN PASTEUR TP.HCM Chủ nhiệm đề tài: TS CAO THỊ BẢO VÂN THÀNH PHỐ HỒ CHÍ MINH-03/2009 Tên đề tài: Giám sát biến đổi gen virút cúm A/H5N1 lưu hành khu vực phía nam năm 2006 – 2008 nghiên cứu ứng dụng quy trình tạo virút cúm giảm độc lực kỹ thuật di truyền ngược chủng Việt Nam Cấp đề tài: Thành phố Cơ quan chủ trì đề tài: Viện Pasteur TPHCM Địa chỉ:167 Pasteur, Quận 3, TPHCM Điện thoại: (848)3820 5131 Cơ quan chủ quản: Sở Khoa học Công nghệ TPHCM Địa chỉ:244 Điện Biên Phủ, Phường 7, Quận 3, TPHCM Điện thoại: ( 848) 9327 831 Tổng kinh phí (triệu đồng): 531 Trong đó, từ ngân sách Nhà nước (triệu đồng):531 Thời gian thực hiện: 24 .tháng, tháng 12./2006 kết thúc 12./2008 Chủ nhiệm đề tài : Họ tên Cao Thị Bảo Vân Học vị: Tiến sĩ Danh sách cá nhân tham gia nghiên cứu (ghi họ tên, học hàm, học vị): Người tham gia A Cơ quan Thời gian tham gia (tháng) Viện Pasteur TPHCM 24 Chủ nhiệm đề tài TS Cao Thị Bảo Vân B Cán tham gia PGS TS Trương Xuân Liên Viện Pasteur TPHCM BS Phan Văn Tú Viện Pasteur TPHCM BS Nguyễn Thanh Long Viện Pasteur TPHCM Ths Võ Hồ Hồng Hải Viện Pasteur TPHCM 24 CN Lê Hà Tầm Dương Viện Pasteur TPHCM 24 CN Nguyễn Thị Yến Nhi Viện Pasteur TPHCM 24 CN Ngô Chung Chỉnh Viện Pasteur TPHCM 24 CN Lê Thị Liên Viện Pasteur TPHCM 24 BS Đồng Mạnh Hòa TT Thú y vùng TPHCM 10 BS Ngô Thanh Long TT Thú y vùng TPHCM Báo cáo tổng kết đề tài MỤC LỤC I ĐẶT VẤN ĐỀ II MỤC TIÊU NGHIÊN CỨU III TOÅNG QUAN TÀI LIỆU III.1 Đại cương virus cúm III.1.1 Phaân loaïi III.1.2 Đặc điểm sinh thái III.1.3 Cấu trúc chức gen III.1.4 Khả biến đổi di truyền virus cúm A III.1.5 Kỹ thuật di truyền ngược nghiên cứu virus cúm III.2 Tình hình dịch tễ nước IV NỘI DUNG NGHIÊN CỨU 12 IV.1 Thiết kế nghiên cứu 12 IV.2 Phương pháp nghiên cứu 12 V KẾT QUẢ – BÀN LUẬN 19 V.1 Kết phân tích gen cúm A 19 V.1.1 Gen HA kháng nguyên hemagglutinin 19 V.1.2 Gen NA kháng nguyên neuraminidase 24 V.1.3 Gen M .26 V.1.4 Gen NS .27 V.1.5 Gen PB2 29 V.1.6 Caùc gen PB1, PA, NP 30 Phòng SHPT-Viện Pasteur TP.HCM Báo cáo tổng kết đề tài V.2 Kết tạo dòng gen 40 V.3 Taïo virus cúm tái tổ hợp kỹ thuật di truyền ngược 41 VI KẾT LUẬN – ĐỀ NGHÒ 45 VII TÀI LIỆU THAM KHẢO 47 Phụ lục 1: Danh sách chủng virus cúm A/H5N1 giải mã gen đề tài 54 Phụ lục 2: Số đăng ký GenBank 56 Phụ lục Trình tự gen virus ………………………………………………… 61 Phụ lục Sự khác biệt chủng tạo với chủng vaccine 93 Phụ lục Xác nhận đăng báo 94 Phòng SHPT-Viện Pasteur TP.HCM Báo cáo tổng kết đề tài DANH MỤC CHỮ VIẾT TẮT aa : axít amin BHI : Brain Heart Infusion bp : cặp bazơ (base pair) CDC : Trung tâm Ngăn ngừa Kiểm soát dịch bệnh (Centers for Disease Control and Prevention) cDNA : DNA boå sung (complementary DNA) dATP : 2’-deoxyadenosine-5’-triphosphate dTTP : 2’-deoxythymidine-5’-triphosphate dGTP : 2’-deoxyguanosine-5’-triphosphate dCTP : 2’-deoxycytidine-5’-triphosphate dNTP : 2’-deoxynucleosid-5’-triphosphate DMEM : Dulbecco’s modified Eagle medium DNA : Axít Deoxyribonucleic DTT : Dithiothreitol EDTA : Ethylene-Di-amine-Tera-acetic axít ER : Lưới nội chất (Endoplasmic Reticulum) FAO : Food and Agriculture Organization HA : Hemagglutinin AI : Virút Influenza A kb : 1000 bazơ (kilo base) MDCK : Madin – Darby canine kidney MEM : Minimum essential medium Eagle mRNA : RNA thoâng tin (messenger RNA) NA : Neuraminidase NCBI : Trung tâm Quốc gia Thông tin Công nghệ sinh học (National Center for Biotechnology Information) Nu : Nucleotide PCR : Phản ứng khuếch đại chuỗi (Polymerase Chain Reaction) RG : Di truyền ngược (reverse genetics) RNA : Axít Ribonucleic RNP : Ribonucleoprotein RT-PCR : Reverse Transcriptase - Polymerase Chain reaction Taq : Enzyme taùch chiết từ vi khuẩn Thermus aquaticus Phòng SHPT-Viện Pasteur TP.HCM i Báo cáo tổng kết đề tài TTTY : Trung tâm thú y vRNA : RNA virút WHO : Tổ chức Y tế Thế giới (World Health Organization) Ký hiệu tỉnh VL HCM TN TV LA AG KG DT BL CM : Vónh Long : TP Hồ chí Minh : Tây Ninh : Trà Vinh : Long An : An Giang : Kiên Giang : Đồng Tháp : Bạc Liêu : Cà Mau Ký hiệu axít amin Ala Asx Cys Asp Glu Phe Gly His Ile Lys Leu Met Asn Pro Gln Arg Ser Thr Val Trp Tyr Glx : Alanine (A) : Aspartic acid hoaëc Asparagine (B) : Cysteine (C) : Aspartic acid (D) : Glutamic acid (E) : Phenylalanine (F) : Glycine (G) : Histidine (H) : Isoleucine (I) : Lysine (K) : Leucine (L) : Methionine (M) : Asparagine (N) : Proline (P) : Glutamine (Q) : Arginine (R) : Serine (S) : Threonine (T) : Valine (V) : Tryptophan (W) : Tyrosine (Y) : Glutamic acid glutamine (Z) Phòng SHPT-Viện Pasteur TP.HCM ii Báo cáo tổng kết đề tài DANH MỤC HÌNH VÀ BẢNG BIỂU HÌNH HÌNH 1: Virus cúm HÌNH 2: Các kiễu đột biến virus cúm A HÌNH 3: Sự hình thành virus cúm hoàn chỉnh từ cDNA dòng hóa HÌNH 4: Bản đồ phân bố vùng dịch cúm A/H5N1 Việt Nam 11 HÌNH 5: Cấu truùc protein HA 19 HÌNH 6: Trình tự axít amin điểm phân cắt HA1-HA2 20 HÌNH 7: Trình tự axít amin cấu trúc vòng 130 22 HÌNH 8: Trình tự đoạn 20 axít amin treân protein NA 24 HÌNH 9: Trình tự axít amin vị trí kháng thuốc protein M 26 HÌNH 10: Sơ đồ cấu trúc protein NS 27 HÌNH 11: Trình tự đoạn axít amin protein NS 28 HÌNH 12: Cây phả heä gen HA 30 HÌNH 13: Cây phả hệ gen NA 31 HÌNH 14: Cây phả hệ gen M 32 HÌNH 15: Cây phả hệ gen NS 33 HÌNH 16: Cây phả hệ gen PB2 34 HÌNH 17: Cây phả hệ gen PB1 35 HÌNH 18: Cây phả hệ gen NP 36 HÌNH 19: Cây phả hệ gen PA 37 HÌNH 20: Genotype virus cuùm A/H5N1 38 HÌNH 21: Kiểm tra kết nối gen HA vào plasmid pHW2000 41 Phòng SHPT-Viện Pasteur TP.HCM iii Báo cáo tổng kết đề tài HÌNH 22: Sơ đồ tạo virus rgA/H5N1/Dk/TV08-HA 42 HÌNH 23: Kết RT-PCR kiểm tra gen sau chuyển nhiễm 43 BẢNG BẢNG 1: Các gen mã hóa protein virus cúm A chức BẢNG 2: Bộ plasmid sử dụng tạo virus cúm A/H5N1 tái tổ hợp 15 BẢNG 3: Tóm tắt nội dung công việc kết thực đề tài 17 BẢNG 4: Vị trí axít amin protein M2 chủng hoang dại 26 BẢNG 5: Danh sách plasmid tạo dòng 39 BẢNG 6: Kết chuyển nhiễm virus rgA/H5N1/Dk/TV08-HA 43 Phòng SHPT-Viện Pasteur TP.HCM iv Báo cáo tổng kết đề tài I ĐẶT VẤN ĐỀ Bệnh cúm bệnh nhiễm trùng đường hô hấp phổ biến nhất, ước tính hàng năm giới có khoảng 10% đến 20% dân số nhiễm bệnh cúm, 3.000.000 đến 5.000.000 trường hợp bị bệnh nặng 250.000 – 500.000 tử vong (WHO, 1999) Tác nhân gây bệnh cúm virus Influenza, gồm ba týp A, B C Virus cúm týp A nghiên cứu nhiều hai týp lại có khả biến đổi cấu trúc di truyền cách mau chóng, nguyên nhân gây trận dịch lan rộng toàn cầu khứ Điển hình, vào kỷ XX, có trận dịch cúm người virus cúm A gây ra: đại dịch “cúm Tây Ban Nha” (H1N1) năm 1918 - 1919 làm 20 - 50 triệu người toàn giới thiệt mạng (riêng Hoa Kỳ có 550.000 người); dịch “cúm Châu Á” (H2N2) năm 1957 - 1958 làm thiệt mạng 70.000 người Hoa Kỳ; dịch “cúm Hong Kong” (H3N2) năm 1968 - 1969 làm thiệt mạng 34.000 người Hoa Kỳ (WHO, 2004) Virus cúm A thuộc họ Orthomyxoviridae, gen bao gồm mảnh RNA mạch đơn, xoắn âm (-ssRNA); gồm 16 thứ týp HA (H1-H16), thứ týp NA (N1-N9) Sự kết hợp thứ týp HA NA tạo nhiều thứ týp khác khả gây bệnh Tất thứ týp có vật chủ tự nhiên loài chim nước hoang dại Ở người thường lưu hành ba thứ týp HA (H1, H2, H3) hai thứ týp NA (N1, N2) (Webby, 2001) Dòng virus cúm H5N1 (một thứ týp virus cúm A) gây dịch gia cầm Hong Kong năm 1997, lần vượt hàng rào vật chủ nhiễm sang người làm thiệt mạng số 18 bệnh nhân.(Subbarao, 1998) Đây dòng virus cực độc, gây nên 87,5 – 100% tử vong thử nghiệm độc tính gà chim Virus cúm týp H5 trước chưa gây bệnh người, hệ miễn dịch người hoàn toàn xa lạ với týp HA này, nguy đại dịch người hoàn toàn có sở virus sau biến đổi lây truyền từ người sang người khác Phòng SHPT-Viện Pasteur TP.HCM Báo cáo tổng kết đề tài Từ cuối năm 2003 đến nay, loạt đợt dịch cúm gia cầm virus cúm A/H5N1 bùng nổ lan rộng nhiều quốc gia giới (thuộc Châu Á, Châu u, Trung Đông Châu Phi), gây thiệt hại nghiêm trọng cho kinh tế ngành chăn nuôi, đe dọa sức khỏe cộng đồng) Việt Nam Indonesia hai quốc gia bị ảnh hưởng nặng nề Tính từ cuối năm 2003 đến đầu năm 2009 có 412 trường hợp dương tính với H5N1 256 trường hợp tử vong giới (WHO, 2009) Các đại dịch cúm lịch sử có nguồn gốc từ virus cúm gia cầm biến thể, virus cúm A/H5N1 độc tính cao xem tác nhân tiềm tàng cho đại dịch cúm xảy kỉ 21 (Peiris, 2007) Những thiệt hại kinh tế gia tăng số nạn nhân nhiễm tử vong virus cúm gia cầm H5N1 cho thấy virus cúm A/H5N1 mối đe dọa thách thức người việc tìm hiểu đặc tính, khả biến đổi di truyền, tính kháng thuốc, chế lan truyền virus từ gia cầm sang người có hay không khả lan truyền bệnh từ người sang người Để kịp thời đánh giá khả bộc phát dịch cúm gia cầm người, quan chức cần triển khai giám sát tiến hóa gen biến thể virus cúm A/H5N1 virus người gia cầm khu vực nói chung nhằm phát cảnh báo sớm đột biến nguy hiểm liên quan đến độc tính virus, tính kháng thuốc, đồng thời tìm hiểu chế cho phép virus cúm gia cầm vượt qua hàng rào ký chủ để lây nhiễm cho người từ người sang người Phòng SHPT-Viện Pasteur TP.HCM Báo cáo tổng kết đề tài >3BL-NP [org=Influenza A virus] [strain=A/DK/Viet Nam/BL-3/2006 (H5N1)] agcaaaagcagggtaaataatcactcaccgagtgacatcaacatcatggcgtctcaaggcaccaaacgatcttatgaacagatggaaactg gtggggaacgccagaatgctactgagatcagggcatctgttggaagaatggttagtggcattgggaggttctacatacagatgtgcacaga actcaaactcagtgactatgaagggaggctgatccagaacagcataacaatagagagaatggtactctctgcatttgatgaaagaaggaac agatacctggaagaacaccccagtgcggggaaggatccaaagaagactggaggcccaatttatcggaggagagatgggaaatgggtga gagagctaattctgtacgacaaagaggagatcaggaggatttggcgtcaagcgaacaatggagaggacgcaactgctggtcttacccacc tgatgatatggcattccaatctaaatgatgccacatatcagagaacgagagctctcgtgcgtactggaatggacccaaggatgtgctctctga tgcaagggtcaactctcccgaggagatctggagctgctggtgcagcagtaaagggggtagggacaatggtgatggagctgattcggatg ataaaacgaggggtcaacgaccggaatttctggagaggcgaaaatggaagaagaacaaggattgcatatgagagaatgtgcaacatcct caaagggaaattccaaacagcagcacaaagagcaatgatggatcaagtgcgagagagcagaaatcctgggaatgctgaaattgaagatc tcatttttctggcacggtctgcactcatcctgagaggatcagtggcccataagtcctgcttgcctgcttgtgtatacggacttgcagtggccagt ggatatgactttgagagagaaggatactctctggttggaatagatcctttccgcctgcttcaaaacagccaggtctttagtctcattagaccaaa tgagaatccagcacataagagtcaattagtgtggatggcatgccactctgcagcatttgaggaccttagagtctcaagtttcatcagagggac aagagtggtcccaagaggacagctatccaccagaggggttcaaattgcttcaaatgagaacatggaggcaatggactccaacactcttgaa ctgagaagcagatattgggctataagaaccagaagcggaggaaacaccaaccagcagaaggcatctgcaggacagatcagtgttcagc ccactttctcggtacagagaaaccttcccttcgaaagagcgaccattatggcagcatttacaggaaatactgagggtagaacgtctgacatg aggactgaaatcataagaatgatggaaagtgccaaaccagaagatgtgtcattccaggggcggggagtcttcgagctctcggacgaaaag gcaacgaacccgatcgtgccttcctttgacatgaataatgaaggatcttatttcttcggagacaatgcagaggagtacgacaattaaggaaaa atacccttgtttctacta >MBP5=NP [org=Influenza A virus] [strain=A/Teal/Viet Nam/MBP5/2006 (H5N1)] agcaaaagggcagggtaaataatcactcactgagtgacatcaacatcatggcgtctcaaggcaccaaacgatcttatgaacagatggaaac tggtggagaacgccagaatgccactgaratcagagcatctgttggaagaatggttggtggaattgggaggttttacatacagatgtgcactg agctcaaactcagcgactatgaaggaaggctgatccagaacagcataacgatagagagaatggtcctctctgcatttgatgaaaggagga acaaatacctggaagaacatcccagcgcagggaaggatccgaagaaaactggaggtccaatttatcgaaggagagacggaaaatggat gagagagctgattctgtatgacaaagaggagatcagaaggatctggcgtcaggcaaacaatggagaagacgcaactgctggcctcactc acctgatgatctggcattccaacctaaatgatgccacataccagagaacaagagctcttgtgcgtactgggatggaccccagaatgtgctct ctgatgcaaggatcaactctcccgaggagatctggagctgctggtgcagcagtaaagggagtcggaacgatggtgatggaactaattcgg atgataaaacgggggattaatgatcggaacttctggagaggtgaaaatggacgaagaacaagaattgcatatgagagaatgtgcaacatcc tcaaagggaaattccaaacagcagcacaaagagcaatgatggatcaagtacgagaaagtaggaatcctggaaatgctgaaattgaagatc tcatytttctagcacggtctgcactcatcctgagaggatcagtggcccataagtcctgcttgccggcttgtgtgtacggacttgcagtggcca gtgggtatgattttgaaagagaaggatattctctagttggaatagatcctttccgcctgcttcagaacagccaggtcttcagcctcattagaccg aacgagaatccagcacacaagagtcaattggtgtggatggcatgccattctgcagcatttgaggacctgagagtctcaagtttcatcagagg aacaagagtagtcccaagaggacaaytatccaccagaggagttcaaattgcctcaaatgagaatatggaaacaatggactccagcactctt gaactgagaagcagatattgggctataagaaccaggagtggaggaaacaccaaccaacagagagcatctgcaggacaaatcagtgtaca acccactttctcagtacagagaaatcttcccttcgaaagagcaaccatcatggcggcattcacagggaatactgagggcagaacatctgac atgaggaccgaaatcataagaatgatggaaaatgccagaccagaagatgtgtctttccaggggcggggagtcttcgagctctcggacgaa aaggcaacgaacccgatcgtgccttcctttgacatgagtaatgaaggatcttatttcttcggagacaatgcagaggagtacgata >9DT-NP [org=Influenza A virus] [strain=A/DK/Viet Nam/DT-9/2007 (H5N1)] aggtcccatttatcggaggagagatgggaaatgggtgagagagctaattctatacgacaaagaggagatcaggaggatttggcgtcaagc gaacaatggagagaacgcaactgctggtcttacccacctgatgatatggcattccaatctaaatgatgccacatatcagagaacgagagctc tcgtgcgtactggaatggacccaaggatgtgctctctgatgcaagggtcaactctcccgaggagatctggagctgctggtgcagcagtaaa gggagtagggacaatggtgatggagctgattcggatgataaaacgaggggtcaacgaccgtaatttctggagaggagaaaatggaagaa gaacaaggattgcatatgagagaatgtgcaacatcctcaaagggaaattccaaacagcagcacaaagagcaatgatggatcaagtgcga gagagcagaaatcctgggaatgctgaaattgaagatctcatttttctggcacggtctgcactcatcctgagaggatcagtggcccataagtcc tgcttgcctgcttgtgtatacggacttgcagtggccagtggatatgactttgagagagaagggtactctctggttggaatagatcctttccgcct gcttcaaaacagccaggtctttagtctcattagaccaaatgagaatccagcacataagagtcaattagtgtggatggcatgccactctgcagc atttgaggaccttagagtctcaagtttcatcagagggacaagagtggtcccaagaggacagctatccaccagaggggttcaaattgcttcaa atgagaacatggaggcaatggactccaacactcttgaactgaggagcagatattgggctataagaaccagaagcggaggaaacaccaac cagcagaaggcatctgcaggacagatcagtgttcagcccactttctcggtacagagaaaccttcccttcgaaagagcgaccattatggcag catttacaggaaatactgagggtagaacgtctgacatgaggactgaaatcataagaatgatggaaagtgccagaccagaagatgtgtcattc Phòng SHPT-Viện Pasteur TP.HCM 79 Báo cáo tổng kết đề tài caggggcggggagtcttcgagctctcggacgaaaaggcaacgaacccgatcgtgccttcctttgacatgaataatgaaggatcttatttcttc ggagaccatgcagaggagtat >12A-LA-NP [org=Influenza A virus] [strain=A/DK/Viet Nam/12A-LA/2008 (H5N1)] gataatcactcaccgagtgacatcaacatcatggcgtctcaaggcaccaaacgatcgtatgaacagatggagactagtggggaacgccag aatgctactgagatcagggcatctgttggaagaatggttagtggcattgggaggttctacatacagatgtgcacagaactcaaactcagtga ctatgaagggaggctgatccagaacagcataacaatagagagaatggtactctctgcatttgatgaaagaaggaacagatacctggaaga acaccccagtgcgggaaaggatccgaagaagactggaggtccaatttatcggaggagagatgggaaatgggtgagagagctaattctgt acgacaaagaggagatcaggaggatttggcgtcaagcgaacaatggagaggacgcaactgctggtcttacacacctgatgatatggcatt ccaatctaaatgatgccacatatcagagaacgagagctctcgtacgtactggaatggacccaaggatgtgctctctgatgcaagggtcaact ctcccgaggagatctggagctgctggtgcagcagtaaagggagtagggacaatggtgatggagctgattcggatgataaaacgaggggt caacgaccggaatttctggagaggcgaaaatggaagaagaacaaggattgcatatgagagaatgtgcaacatcctcaaagggaaattcca aacagcagcacaaagagcaatgatgattcaagtgcgagagagcagaaatcctgggaatgctgaaattgaagacctcatttttctagcacgg tctgcactcatcctgagaggatcagtggcccataagtcctgcttgcctgcttgtgtatacggacttgcagtggccagtggatatgactttgaga gagaaggatactctctggttggaatagatcctttccgcctgcttcaaaacagccaggtctttagtctcattagaccaaatgagaatccagcaca taagagtcaattagtgtggatggcatgccactctgcagcatttgaggaccttagagtctcaagtttcatcagagggacaagagtggtcccaa gaggacagctatccaccagaggggttcaaattgcttcaaatgagaacatggaggcaatggactccaacactcttgaactgagaagcagata ttgggctataagaaccagaagcggaggaaacaccaaccaccagaaggcatctgcaggacagatcagtgttcagcccactttctcggtaca gagaaaccttcccttcgaaagagcgaccattatggcagcatttacaggaaatactgagggtagaacgtctgacatgaggactgaaatcata agaatgatggaaagtgccagaccagaagatgtgtcattccaggggcggggagtcttcgagctctcggacgaaaaggcaacgaacccgat cgtgccttcctttgacatgaataatgaaggatcttatttcttcggagacaatgcagaggagtatga >15A-TV-NP [org=Influenza A virus] [strain=A/DK/Viet Nam/15A-TV/2008 (H5N1)] agcaaaagcaggttagataatcactcaccgagtgacatcaacatcatggcgtctcaaggcaccaaacgatcttatgaacagatggagacta gtggggaacgccagaatgctactgagatcagggcatctgttggaagaatggttagtggcattgggaggttctacatacagatgtgcacaga actcaaactcagtgactatgaagggaggctgatccagaacagcataacaatagagagaatggtactctctgcatttgatgaaagaaggaac agatacctggaagaacaccccagtgcgggaaaggatccaaaaaagactggaggtccaatttatcggaggagagatgggaaatgggtga gagagctaattctgtacgacaaagaggagatcaggaggatttggcgtcaagcgaacaatggagaggacgcaactgctggtcttacccacc tgatgatatggcattccaatctaaatgatgccacatatcagagaacgagagctctcgtgcgtactggaatggacccaaggatgtgctctctga tgcaagggtcaactctcccgaggagatctggagctgctggtgcagcagtaaagggagtagggacaatggtgatggagctgattcggatga taaaacgaggggtcaacgaccggaatttctggagaggcgaaaatggaagaagaacaaggattgcatatgagagaatgtgcaacatcctc aaagggaaattccaaacagcagcacaaagagcaatgatggatcaagtgcgagagagcagaaatcctgggaatgctgaaattgaagacct catttttctggcacggtctgcactcatcctgagaggatcagtggcccataagtcctgcttgcctgcttgtgtatacggacttgctgtggccagtg gatatgactttgagagagaaggatactctctggttggaatagatcctttccgcctgcttcaaaacagccaggtctttagtctcattagaccaaat gagaatccagcacataagagtcaattagtgtggatggcatgccactctgcagcatttgaggaccttagagtctcaagtttcatcagagggac aagagtggtcccaagaggacagctatccaccagaggggttcaaattgcttcaaatgagaacatggaggcaatggactccaacactcttgaa ctgagaagcagatattgggctataagaaccagaagcggaggaaacaccaaccaccagaaggcatctgcaggacagatcagtgttcagcc cactttctcggtacagagaaaccttcccttcgaaagagcgaccattatggcagcatttacaggaaatactgagggtagaacgtctgacatga ggactgaaatcataagaatgatggaaagtgccagaccagaagatgtgtcattccaggggcggggagtcttcgagctctcggacgaaaag gcaacgaacccgatcgtgccttcctttgacatgaataatgaaggatcttatttcttcggagacaatgcagaggagtacgacaattaaggaaaa ataccct >17A-TV-NP [org=Influenza A virus] [strain=A/Dk/Viet Nam/17A-TV/2008 (H5N1)] atcactcaccgagtgacatcaacatcatggcgtctcaaggcaccaaacgatcttatgaacagatggagactagtggggaacgccagaatg ctactgagatcagggcatctgttggaagaatggttagtggcattgggaggttctacatacagatgtgcacagaactcaaactcagtgactatg aagggaggctgatccagaacagcataacaatagagagaatggtactctctgcatttgatgaaagaaggaacagatacctggaagaacacc ccagtgcgggaaaggatccaaaaaagactggaggtccaatttatcggaggagagatgggaaatgggtgagagagctaattctgtacgac aaagaggagatcaggaggatttggcgtcaagcgaacaatggagaggacgcaactgctggtcttacccacctgatgatatggcattccaatc taaatgatgccacatatcagagaacgagagctctcgtgcgtactggaatggacccaaggatgtgctctctgatgcaagggtcaactctcccg aggagatctggagctgctggtgcagcagtaaagggagtagggacaatggtgatggagctgattcggatgataaaacgaggggtcaacga ccggaatttctggagaggcgaaaatggaagaagaacaaggattgcatatgagagaatgtgcaacatcctcaaagggaaattccaaacagc agcacaaagagcaatgatggatcaagtgcgagagagcagaaatcctgggaatgctgaaattgaagacctcatttttctggcacggtctgca ctcatcctgagaggatcagtggcccataagtcctgcttgcctgcttgtgtatacggacttgctgtggccagtggatatgactttgagagagaa ggatactctctggttggaatagatcctttccgcctgcttcaaaacagccaggtctttagtctcattagaccaaatgagaatccagcacataaga Phòng SHPT-Viện Pasteur TP.HCM 80 Báo cáo tổng kết đề tài gtcaattagtgtggatggcatgccactctgcagcatttgaggaccttagagtctcaagtttcatcagagggacaagagtggtcccaagagga cagctatccaccagaggggttcaaattgcttcaaatgagaacatggaggcaatggactccaacactcttgaactgagaagcagatattggg ctataagaaccagaagcggaggaaacaccaaccaccagaaggcatctgcaggacagatcagtgttcagcccactttctcggtacagaga aaccttcccttcgaaagagcgaccattatggcagcatttacaggaaatactgagggtagaacgtctgacatgaggactgaaatcataagaat gatggaaagtgccagaccagaagatgtgtcattccaggggcggggagtcttcgagctctcggacgaaaaggcaacgaacccgatcgtgc cttcctttgacatgaataatgaaggatcttatttcttcggagacaatgcagaggagtacgacaattaaggaaaa >2B-TV-NP [org=Influenza A virus] [strain=A/DK/Viet Nam/2B-TV/2008 (H5N1)] atcactcaccgagtgacatcaacatcatggcgtctcaaggcaccaaacgatcttatgaacagatggagactagtggggaacgccagaatg ctactgagatcagggcatctgttggaagaatggttagtggcattgggaggttctacatacagatgtgcacagaactcaaactcagtgactatg aagggaggctgatccagaacagcataacaatagagagaatggtactctctgcatttgatgaaagaaggaatagatacctggaagaacacc ccagtgcgggaaaggatccaaaaaagactggaggtccaatttatcggaggagagatgggaaatgggtgagagagctaattctgtacgac aaagaggagatcaggaggatttggcgtcaagcgaacaatggagaggacgcaactgctggtcttacccacctgatgatatggcattccaatc taaatgatgccacatatcagagaacgagagctctcgtgcgtactggaatggacccaaggatgtgctctctgatgcaagggtcaactctcccg aggagatctggagctgctggtgcagcagtaaagggggtagggacaatggtgatggagctgattcggatgataaaacgaggggtcaacga ccggaatttctggagaggcgaaaatggaagaagaacaaggattgcatatgagagaatgtgcaacatcctcaaagggaaattccaaacagc agcacaaagagcaatgatggatcaagtgcgagagagcagaaatcctgggaatgctgagattgaagacctcatttttctggcacggtctgca ctcatcctgagaggatcagtggcccataagtcctgcttgcctgcttgtgtatacggacttgcagtggccagtggatatgactttgagagagaa ggatactctctggttggaatagatcctttccgcctgcttcaaaacagccaggtctttagtctcattagaccaaatgagaatccagcacataaga gtcaattagtgtggatggcatgccactctgcagcatttgaggaccttagagtctcaagtttcatcagagggacaagagtggtcccaagagga cagctatccaccagaggggttcaaattgcttcaaatgagaacatggaggcaatggactccaacactcttgaactgagaagcagatattggg ctataagaaccagaagcgggggaaacaccaaccaccagaaggcatctgcaggacagatcagtgttcagcccactttctcggtacagaga aaccttcccttcgaaagagcgaccattatggcagcatttacaggaaatactgagggtagaacgtctgacatgaggactgaaatcataagaat gatggaaagtgccagaccagaagatgtgtcattccaggggcggggagtcttcgagctctcggacgaaaaggcaacgaacccgatcgtgc cttcctttgacatgaataatgaaggatcttatttcttcggagacaatgcagaggagtattacaattaa >36ST-NP [org=Influenza A virus] [strain=A/DK/Viet Nam/36ST/2008 (H5N1)] atcactcaccgagtgacatcaacatcatggcgtctcaaggcaccaaacgatcttatgaacagatggagactagtggggaacgccagaatg ctactgagatcagggcatctgttggaagaatggttagtggcattgggaggttctacatacagatgtgcacagaactcaaactcagtgactatg aagggaggctgatccagaacagcataacaatagagagaatggtactctctgcatttgatgaaagaaggaacagatacctggaagaacacc ccagtgcgggaaaggatccaaaaaagactggaggtccaatttatcggaggagagatgggaaatgggtgagagagctaattctgtacgac aaagaggagatcaggaggatttggcgtcaagcgaacaatggagaggactcaactgctggtcttacccacctgatgatatggcattccaatc taaatgatgccacatatcagagaacgagagctctcgtgcgtactggaatggacccaaggatgtgctctctgatgcaagggtcaactctcccg aggagatctggagctgctggtgcagcagtaaagggagtagggacaatggtgatggagctgattcggatgataaaacgaggggtcaacga ccggaatttctggagaggcgaaaatggaagaagaacaaggattgcatatgagagaatgtgcaacatcctcaaagggaaattccaaacagc agcacaaagagcaatgatggatcaagtgcgagagagcagaaatcctgggaatgctgaaattgaagatctcatttttctggcacggtctgca ctcatcctgagaggatcagtggcccataagtcctgcttgcctgcttgtgtatacggacttgcagtggccagtggatatgactttgagagagaa ggatactctctggttggaatagatcctttccgcctgcttcaaaacagccaggtctttagtctcattagaccaaatgagaatccagcacataaga gtcaattagtgtggatggcatgccactctgcagcatttgaggaccttagagtctcaagtttcatcagagggacaagagtggtcccaagagga cagctatccaccagaggggttcaaattgcttcaaatgagaacatggaggcaatggactccaacactcttgaactgagaagcagatattggg ctataagaaccagaagcggaggaaacaccaaccaccagaaggcatctgcaggacagatcagtgttcagcccactttctcggtacagaga aaccttcccttcgaaagagcgaccattatggcagcatttacaggaaatactgagggtagaacgtctgacatgaggactgaaatcataagaat gatggaaagtgccagaccagaagatgtgtcattccaggggcggggagtcttcgagctctcggacgaaaaggcaacgaacccgatcgtgc cttcctttgacatgaataatgaaggatcttatttcttcggagacaatgcagaggagtacgacaattaaggaaaaatacctg >38ST-NP [org=Influenza A virus] [strain=A/DK/Viet Nam/38ST/2008 (H5N1)] agtgacatcaacatcatggcgtctcaaggcaccaaacgatcttatgaacagatggagactagtggggaacgccagaatgctactgagatca gggcatctgttggaagaatggttagtggcattgggaggttctacatacagatgtgcacagaactcaaactcagtgactatgaagggaggctg atccagaacagcataacaatagagagaatggtactctctgcatttgatgaaagaaggaacagatacctggaagaacaccccagtgcggga aaggatccaaaaaagactggaggtccaatttatcggaggagagatgggaaatgggtgagagagctaattctgtacgacaaagaggagat caggaggatttggcgtcaagcgaacaatggagaggactcaactgctggtcttacccacctgatgatatggcattccaatctaaatgatgcca catatcagagaacgagagctctcgtgcgtactggaatggacccaaggatgtgctctctgatgcaagggtcaactctcccgaggagatctgg agctgctggtgcagcagtaaagggagtagggacaatggtgatggagctgattcggatgataaaacgaggggtcaacgaccggaatttctg gagaggcgaaaatggaagaagaacaaggattgcatatgagagaatgtgcaacatcctcaaagggaaattccaaacagcagcacaaaga Phòng SHPT-Viện Pasteur TP.HCM 81 Báo cáo tổng kết đề tài gcaatgatggatcaagtgcgagagagcagaaatcctgggaatgctgaaattgaagatctcatttttctggcacggtctgcactcatcctgaga ggatcagtggcccataagtcctgcttgcctgcttgtgtatacggacttgcagtggccagtggatatgactttgagagagaaggatactctctg gttggaatagatcctttccgcctgcttcaaaacagccaggtctttagtctcattagaccaaatgagaatccagcacataagagtcaattagtgt ggatggcatgccactctgcagcatttgaggaccttagagtctcaagtttcatcagagggacaagagtggtcccaagaggacagctatccac cagaggggttcaaattgcttcaaatgagaacatggaggcaatggactccaacactcttgaactgagaagcagatattgggctataagaacc agaagcggaggaaacaccaaccaccagaaggcatctgcaggacagatcagtgttcagcccactttctcggtacagagaaaccttcccttc gaaagagcgaccattatggcagcatttacaggaaatactgagggtagaacgtctgacatgaggactgaaatcataagaatgatggaaagtg ccagaccagaagatgtgtcattccaggggcggggagtcttcgagctctcggacgaaaaggcaacgaacccgatcgtgccttcctttgacat gaataatgaaggatcttatttcttcggagacaatgcagaggag >41ST-NP [org=Influenza A virus] [strain=A/DK/Viet Nam/41ST/2008 (H5N1)] agcaaaagcagggttaataatcactcaccgagtgacatcaacatcatggcgtctcaaggcaccaaacgatcttatgaacagatggagacta gtggggaacgccagaatgctactgagatcagggcatctgttggaagaatggttagtggcattgggaggttctacatacagatgtgcacaga actcaaactcagtgactatgaagggaggctgatccagaacagcataacaatagagagaatggtactctctgcatttgatgaaagaaggaac agatacctggaagaacaccccagtgcgggaaaggatccaaaaaagactggaggtccaatttatcggaggagagatgggaaatgggtga gagagctaattctgtacgacaaagaggagatcaggaggatttggcgtcaagcgaacaatggagaggactcaactgctggtcttacccacct gatgatatggcattccaatctaaatgatgccacatatcagagaacgagagctctcgtgcgtactggaatggacccaaggatgtgctctctgat gcaagggtcaactctcccgaggagatctggagctgctggtgcagcagtaaagggagtagggacaatggtgatggagctgattcggatgat aaaacgaggggtcaacgaccggaatttctggagaggcgaaaatggaagaagaacaaggattgcatatgagagaatgtgcaacatcctca aagggaaattccaaacagcagcacaaagagcaatgatggatcaagtgcgagagagcagaaatcctgggaatgctgaaattgaagatctc atttttctggcacggtctgcactcatcctgagaggatcagtggcccataagtcctgcttgcctgcttgtgtatacggacttgcagtggccagtg gatatgactttgagagagaaggatactctctggttggaatagatcctttccgcctgcttcaaaacagccaggtctttagtctcattagaccaaat gagaatccagcacataagagtcaattagtgtggatggcatgccactctgcagcatttgaggaccttagagtctcaagtttcatcagagggac aagagtggtcccaagaggacagctatccaccagaggggttcaaattgcttcaaatgagaacatggaggcaatggactccaacactcttgaa ctgagaagcagatattgggctataagaaccagaagcggaggaaacaccaaccaccagaaggcatctgcaggacagatcagtgttcagcc cactttctcggtacagagaaaccttcccttcgaaagagcgaccattatggcagcatttacaggaaatactgagggtagaacgtctgacatga ggactgaaatcataagaatgatggaaagtgccagaccagaagatgtgtcattccaggggcggggagtcttcgagctctcggacgaaaag gcaacgaacccgatcgtgccttcctttgacatgaataatgaaggatcttatttcttcggagacaatgcagaggagtacgacaattaaggaaaa atacccttgtttctacta GEN NA >1CM-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/CM-1/2006 (H5N1)] tcaaaagcaggagattaaaatgaatccaaatcagaagataataaccatcggatcaatctgtatggtaactggaatagttagcttaatgttacaa gttgggaacatgatctcaatatgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcttactg agaaagctgtggcttcagtaaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagtataagga tcggttccaaaggggatgtgtttgttataagagagccgttcatctcatgctcccacttggaatgcagaactttctttttgactcagggagccttgc tgaatgataaacactccaatgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccatat aactcaaggtttgagtctgttgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatgggg ctgtggctgtattgaaatacaatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcat gtgtaaatggctcttgctttactgtaatgactgatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgat taaatcagtcgaattgaatgctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgtgtgtgcagggataatt ggcatggctcaaatcggccatgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccac gccccaatgatggaaaaggtagttgtggtccggtgtcctctaacggggcatatggggtaaaagggttttcatttaaatacggcaatggtgtct ggatcgggagaaccaaaagcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttc agtgaaacaagatatcgtagcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataa gaccttgtttctgggttgagttgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaaata gtgacactgtgggttggtcttggccagacggtgctgagttgccattcaccattgacaagtagttgttcaaaaaaactccttgtttctacttaattc g >MBP5-NA [org=Influenza A virus] [strain=A/Teal/Viet Nam/MBP5/2006 (H5N1)] agcaaaagcaggagtttaaaatgaatccaaatcagaagataataaccattggatcaatctgtatggtaattggaatagtcagcttgatgttaca aattgggaacataatctcaatatgggttagtcattcaattcaaacagggaatcagcaccaacctgaaccatgcaatcaaagcatcattacttat Phòng SHPT-Viện Pasteur TP.HCM 82 Báo cáo tổng kết đề tài gaaaacaacacctgggtaaaccagacatatgtcaacatcagctataccaattttcctactgagcagactgtgatttcagtggcattagcgggt aattcatctctttgcccaattagtggatgggctatatacagtaaggacaacggtataagaattggttccaaaggggatgtgtttgttataagaga gccattcatctcatgttcccacctggaatgcagaaccttctttttgactcagggagccttattgaatgacaagcattctaatgggaccgtcaaag acagaagcccttatagaaccctaatgagttgccctgtgggtgaggctccttctccatataattcaaggtttgagtctgttgcttggtcggcaagt gcttgtcatgatggcattagctggttgacaattggaatttctggtccagacaatggggcagttgctgtattgaaatacaatggcataataacag acacgatcaagagttggaggaacaacataatgagaacccaagagtctgaatgtgcatgtgtaaatggctcttgcttcactgtaatgactgac gggccaagtagcggacaggcttcatacaaaattttcaagatagagaaagggaaagtagttaaatcagttgaattaaatgctcctaattaccac tacgaggaatgttcctgttatcctgatgctggtgaaatcatgtgtgtatgtagggataattggcatggctcaaatcggccatgggtatcgttcaa tcagaatttggagtatcaaataggctatatatgcagtggagttttcggagacaatccacgccccagtgatggaacaggcagttgcggtccag tgtcctctaacggggcatacggagtgaaagggttttctttcaaatatggcaatggagtttggattgggaggaccaaaagcactagctccaga agcggctttgaaatgatttgggatccaaatgggtggacagaaacagacaatagcttctcagtgaagcaagatattgtagcaataactgactg gtcagggtatagtgggagttttgtccagcacccagaactaacaggattagattgcatgagaccttgtttttgggttgagttaatcagaggacgg cccaaagagaacacaatttggactagtgggagcagcatatccttttgtggtgtaaatagtgacactgtgggttggtcttggccagacggtgct gagttgccattcaccattgacaagtagtttgttcaaaaaactccttgtttctac >9DT-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/DT-9/2007 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatggcaactggaatagttagcttaatgttacaagttgggaacatgatctcaat atgggtcagtcattcaactcacacagggactcaacaccaagctgatccaatcagcaatactaattttcttactgagaaagctgtggcttcagta aaaatagtgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagtataaggatcggttccaagggggatgt gtttgttataagagagccgttcatctcatgctcccacttggaatgcagaactttctttttgactcagggagccttgctgaatgacaaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccatataactcaaggtttgagtctgtt gcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgactgatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcaaattggat gctcctaattatcactataaggaatgctcctgttatcctgacgccggcgaaatcatatgtgtttgcagggataattggcatggctcaaatcggc catgggtatctttcaatcaaaatttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaacagg tagttgtggtccggtgtcctctaacggggcatatggggtaaaagggtttttatttaaatacggcaatggtgtctggatcgggagaaccaaaag cactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacggacagtagattttcagtgaaacaagatatcgtagc aataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagtta atcagagggcggcccaaagagagcacaatttggactagtgggagcagcatatctttttgtggtgtaaatagtgacactgtgggttggtcttgg ccagacggtgctgagttgccattcaccattgacaagtag >12A-LA-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/12A-LA/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatggtaactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatatcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tggaactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgtt gcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgacagatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattgga tgctcctaattatcactatgaggaatgctcctgttatcctgatgccggtgaaatcacatgtgtgtgcagggataattggcatggctcaaatcgac catgggtatctttcaatcaaaacttggagtatcaaatagggtatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaag gtagttgtggtccggtgtcctctaacggggcatatggggtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaaa gcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatattgtag caataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagtt gatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtctt ggccagacggtgctgagttgccattcaccattgacaagtag >15A-TV-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/15A-TV/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatggtaactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatctcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa Phòng SHPT-Viện Pasteur TP.HCM 83 Báo cáo tổng kết đề tài tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgacagatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattgga tgctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgtgtgtgcagggataattggcatggctcaaatcga ccatgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaa ggtagttgtggtccggtgtcctctaacggggcatatggggtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaa agcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgta gcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagt tgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtct tggccagacggtgctgagttgccattcaccattgacaagtagttgttcaaaaaactccttgtttctact >17A-TV-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/17A-TV/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatggtaactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgttcgttataagagagccattcatctcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactcca atgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctg ttgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaata caatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgctt tactgtaatgacagatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattgg atgctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgtgtgtgcagggataattggcatggctcaaatcg accatgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaa aggtagttgtggtccggtgtcctctaacggggcatatggggtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaa aagcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgt agcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgag ttgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtc ttggccagacggtgctgagttgccattcaccattgacaagtagttgttcaaaaaactccttggtttctac >2B-TV-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/2B-TV/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatggtaactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatatcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgactgatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattggat gctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgcgtgtgcagggataattggcatggctcaaatcgac catgggtatctttcgatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaag gtagttgtggtccggtgtcctctaacggggcatatggggtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaaa gcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgtag caataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcatcagacctcgtttctggattgagtt gatcagagggcggcccaaagagggcacgatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtctt ggccagacggtgctgagttgccattcaccattgacaagtag >36ST-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/36ST/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatgataactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatctcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt Phòng SHPT-Viện Pasteur TP.HCM 84 Báo cáo tổng kết đề tài actgtaatgactgatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattggat gctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgtgtgtgcagggataattggcatggctcaaatcgac catgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaag gtagttgtgggccggtgtcctctaacggggcatatggagtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaa agcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgta gcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagt tgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtct tggccagacggtgctgagttgccattcaccattgacaagtagttgttcaaaaaactccttggtttctac >38ST-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/38ST/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatgataactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatatcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgactgatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattggat gctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgtgtgtgcagggataattggcatggctcaaatcgac catgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaag gtagttgtgggccggtgtcctctaacggggcatatggagtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaa agcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgta gcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagt tgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtct tggccagacggtgctgagttgccattcaccattgacaagtag >41ST-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/41ST/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatgataactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatctcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgactgatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattggat gctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgtgtgtgcagggataattggcatggctcaaatcgac catgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaag gtagttgtgggccggtgtcctctaacggggcatatggagtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaa agcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgta gcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagt tgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtct tggccagacggtgctgagttgccattcaccattgacaagtagttgttcaaaaaactccttgtttctac >40ST-NA [org=Influenza A virus] [strain=A/DK/Viet NaM/40ST/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatgataactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatatcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgactgatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattggat gctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgtgtgtgcagggataattggcatggctcagatcgac catgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaag Phòng SHPT-Viện Pasteur TP.HCM 85 Báo cáo tổng kết đề tài gtagttgtgggccggtgtcctctaacggggcatatggagtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaa agcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgta gcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagt tgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtct tggccagacggtgctgagttgccattcaccattgacaagtag >19A-TV-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/19A-TV/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatggtaactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatatcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgacagatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattgga tgctcctaattatcactatgaggaatgctcgtgttatcctgatgccggcggaatcacatgtgtgtgcagggataattggcatggctcaaatcga ccatgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaa ggtagttgtggtccggtgtcctctaacggggcatatggggtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaa agcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgta gcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagt tgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtct tggccagacggtgctgagttgccattcaccattgacaagtag >20A-TV-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/20A-TV/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatggtaactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatatcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgacagatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattgga tgctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgtgtgtgcagggataattggcatggctcaaatcga ccatgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaa ggtagttgtggtccggtgtcctctaacggggcatatggggtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaa agcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgta gcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagt tgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtct tggccagacggtgctgagttgccattcaccattgacaagtag >11A-LA-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/11A-LA/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatggtaactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatatcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tggaactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgtt gcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgacagatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattgga tgctcctaattatcactatgaggaatgctcctgttatcctgatgccggtgaaatcacatgtgtgtgcagggataattggcatggctcaaatcgac catgggtatctttcaatcaaaacttggagtatcaaatagggtatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaag gtagttgtggtccggtgtcctctaacggggcatatggggtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaaa gcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatattgtag caataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagtt Phòng SHPT-Viện Pasteur TP.HCM 86 Báo cáo tổng kết đề tài gatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtctt ggccagacggtgctgagttgccattcaccattgacaagtag >39ST-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/39ST/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatgataactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatatcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgactgatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattggat gctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgtgtgtgcagggataattggcatggctcaaatcgac catgggtatctttcaatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaag gtagttgtgggccggtgtcctctaacggggcatatggagtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaa agcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgta gcaataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagt tgatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtct tggccagacggtgctgagttgccattcaccattgacaagtag >7B-TV-NA [org=Influenza A virus] [strain=A/DK/Viet Nam/7B-TV/2008 (H5N1)] atgaatccaaatcagaagataataaccatcggatcaatctgtatggtaactggaatagttagcttaatgttacaagttgggaacatgatctcgat atgggtcagtcattcaattcacacagggaatcaacaccaagctgagccaatcagcaatactaattttcctactgagaaagctgtggcctcagc aaaattagcgggcaattcatctctttgccccattaacggatgggctgtatacagtaaggacaacagcataaggatcggttccaaaggggatg tgtttgttataagagagccattcatatcatgctcccacttggaatgcagaactttctttttgactcaaggagccttgctgaatgataaacactccaa tgggactgtcaaagacagaagccctcacagaacattaatgagttgtcctgtgggtgaggctccctccccgtataactcaaggtttgagtctgt tgcttggtcagcaagtgcttgccatgatggcaccagttggttgacaattggaatttctggcccagacaatggggctgtggctgtattgaaatac aatggcataataacagacactatcaagagttggaggaacaacatactgagaactcaagagtctgaatgtgcatgtgtaaatggctcttgcttt actgtaatgactgatggaccaagtaatggtcaggcatcacataagatcttcaaaatggaaaaaggaaaagtgattaaatcagtcgaattggat gctcctaattatcactatgaggaatgctcctgttatcctgatgccggcgaaatcacatgcgtgtgcagggataattggcatggctcaaatcgac catgggtatctttcgatcaaaacttggagtatcaaataggatatatatgcagtggggttttcggagacaatccacgccccaatgatggaaaag gtagttgtggtccggtgtcctctaacggggcatatggggtaaaagggttttcatttaaatacggcaatggtgtctggatcgggagaaccaaaa gcactaattccaggagcggctttgaaatgatttgggatccaaatgggtggactgaaacagacagtagcttttcagtgaaacaagatatcgtag caataactgattggtcaggatatagcgggagttttgtccagcatccagaactgacaggactagattgcataagaccttgtttctgggttgagtt gatcagagggcggcccaaagagggcacaatttggactagtgggagcagcatatctttctgtggtgtaagtggtgacactgtgggttggtctt ggccagacggtgctgagttgccattcaccattgacaagtag GEN M >1CM-M [org=Influenza A virus] [strain=A/Dk/Viet Nam/CM-1/2006(H5N1)] atgagtcttctaaccgaggtcgaaacgtacgttctctctatcatcccgtcaggccccctcaaagccgagatcgcgcagaaacttgaggatgt ctttgcaggaaagaacacagatcttgaggctctcatggagtggctaaagacaagaccaatcctgtcacctctgactaaagggatcttgggat ttgtattcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccagaacgccctaaatggaaatggagatccaaataa catggatagggcagttaagctatataagaagctgaaaagagaaataacattccatggggctaaggaggtcgcactcagctactcaaccggt gcacttgccagttgcatgggtctcatatacaacaggatgggaacggtgactacagaagtggcttttggcctagtgtgtgccacttgtgagca gattgcagattcacagcatcggtctcacagacagatggcaactatcaccaacccactaatcagacatgaaaacagaatggtgctggccagc actacagctaaggctatggagcagatggcgggatcaagtgagcaggcagcggaagccatggagatcgctaatcaggctaggcagatgg tgcaggcaatgaggacaattgggactcatcctaactctagtgctggtctgagagataatcttcttgaaaatttgcaggcctaccagaaacgaa tgggagtgcagatgcagcgattcaagtgatcctattgttgttgccgcaaatatcattgggatcttgcacttgatattgtggattcttgatcgtctttt cttcaaatgcatttatcgtcgccttaaatacggtttgaaaagaggccctgctacggcagggatacctgagtctatgagggaagagtaccggc aggaacagcagagtgctgtagatgttgacgatggtcattttgtcaacatagaattggagtaa >2CM-M [org=Influenza A virus] [strain=A/Dk/Viet Nam/CM-2/2006(H5N1)] Phòng SHPT-Viện Pasteur TP.HCM 87 Báo cáo tổng kết đề tài atgagtcttctaaccgaggtcgaaacgtacgttctctctatcatcccgtcaggccccctcaaagccgagatcgcgcagaaacttgaggatgt ctttgcaggaaagaacacagatcttgaggctctcatggagtggctaaagacaagaccaatcctgtcacctctgactaaagggatcttgggat ttgtattcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccagaacgccctaaatggaaatggagatccaaataa catggatagggcagttaagctatataagaagctgaaaagagaaataacattccatggggctaaggaggtcgcactcagctactcaaccggt gcacttgccagttgcatgggtctcatatacaacaggatgggaacggtgactacagaagtggcttttggcctagtgtgtgccacttgtgagca gattgcagattcacagcatcggtctcacagacagatggcaactatcaccaacccactaatcagacatgaaaacagaatggtgctggccagc actacagctaaggctatggagcagatggcgggatcaagtgagcaggcagcggaagccatggagatcgctaatcaggctaggcagatgg tgcaggcaatgaggacaattgggactcatcctaactctagtgctggtctgagagataatcttcttgaaaatttgcaggcctaccagaaacgaa tgggagtgcagatgcagcgattcaagtgatcctattgttgttgccgcaaatatcattgggatcttgcacttgatattgtggattcttgatcgtctttt cttcaaatgcatttatcgtcgccttaaatacggtttgaaaagaggccctgctacggcagggatacctgagtctatgagggaagagtaccggc aggaacagcagagtgctgtagatgttgacgatggtcattttgtcaacatagaattggag >MBP5-M I [org=Influenza A virus] [strain=A/teal/Viet Nam/MBP5/2007 (H5N1)] atgagtcttctaaccgaggtcgaaacgtatgttctctctattgtcccgtcaggccccctcaaagccgagatcgcgcagagacttgaagatgtc tttgcagggaagaacactgatcttgaggctctcatggaatggctaaagacaagaccaatcctgtcacctctgactaaggggattttagggttt gtgttcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccaaaatgccctaaatggaaatggagacccgaacaac atggacagggcagtcaaactgtataggaagctgaaaagagagataacattccatggagctaaagaagtcgcactcagttactcaaccggt gcacttgccagttgtatgggcctcatatacaacagaatggggacggtgaccacagaagtggcttttggcctagtgtgtgctacctgtgagca gattgctgattcacagcatcgatcccacaggcagatggtaactaccaccaacccactaatcaggcatgaaaacagaatggtgctggccagc actacagctaaggctatggagcagatggctgggtcgagtgagcaagcagcggaggccatggaggttgctagtcaggctaggcagatggt gcaggcgatgagaacaattggaactcaccctagctccagtgccggtctgaaagatgatcttcttgaaaatttgcaggcctaccagaaacgg atgggagtgcagatgcagcgattcaagtgatcctctcgttattgccgcaagtatcattgggatcttgcacttgatattgtggattcttgatcgtctt ttcttcaaatgtatttatcgtcgccttaaatacggtttgaaaagagggccttctacggaaggagtgcctgagtctatgagggaagagtatcggc aggaacagcagagtgctgtggatgttgacgatggtcattttgtcaacatagaattggagtaa >9DT-M [org=Influenza A virus] [strain=A/DK/VN/9-DT/2007 (H5N1)] atgagtcttctaaccgaggtcgaaacgtacgttctctctatcatcccgtcaggccccctcaaagccgagattgcgcagaaacttgaggatgtc tttgcaggaaagaacacagatctcgaggctctcatggagtggctgaagacaagaccaatcctgtcacctctgactaaagggattttgggattt gtattcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccagaacgccctaaatggaaatggagatccaaataata tggatagggcagttaagctatataagaagctgaaaagagaaataacattccatggggctaaggaggtcgcactcagctactcaaccggtgc acttgccagttgcatgggtctcatatacaacaggatgggaacagtgactacagaagtggcttttggcctagtgtgtgccacttgtgagcagat tgcagattcacagcatcggtctcacagacagatggcaactatcaccaacccactaatcagacatgagaacagaatggtgctggccagcact acagctaaggctatggagcagatggcgggatcaagtgagcaggcagcggaagccatggagatcgctaatcaggctaggcagatggtgc aggcaatgaggacaattgggactcatcctaactctagtgctggtctgagagataatcttcttgaaaatttgcaggcctaccagaaacgaatgg gagtgcagatgcagcgattcaagtgatcctattgctgttgccgcaaatatcattgggatcttgcacttgatattgtggattcttgatcgtcttttctt caaatgcatttatcgtcgccttaaatacggtttgaaaagaggccctgctacggcaggggtacctgagtctatgagggaagagtaccggcag gaacagcagagtgctgtggatgttgacgatggtcattttgtcaacatagaattggagta>12A-LA-M [org=Influenza A virus] [strain=A/DK/VN/12A-LA/2008 (H5N1)] agatgagtcttctaaccgaggtcgaaacgtacgttctctctatcatcccgtcaggccccctcaaagccgagatcgcgcagaaacttgaggat gtctttgcaggaaagaacacggatcttgaggctctcatggagtggctaaagacaagaccaatcctgtcacctctgactaaagggatcttggg atttgtattcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccagaacgccctaaatggaaatggagatccaaata acatggatagggcagttaagctatataagaagctgaaaagagaaataacattccatggggctaaggaggtcgcactcagctactcaaccg gtgcacttgccagttgcatgggtctcatatacaacaggatgggaacggtgactacagaagtggcttttggcctagtgtgtgccacttgtgagc agattgcagattcacagcatcggtctcacagacagatggcaactatcaccaacccactaatcagacatgaaaacagaatggtgctggccag cactacagctaaggctatggagcagatggcgggatcaagtgagcaggcagcggaagccatggagatcgctaatcaggctaggcagatg gtgcaggcaatgaggacaattgggactcatcctaattctagtactggtctgagagataatcttcttgaaaatttgcaggcctaccagaaacga atgggagtgcagatgcagcgattcaagtgatcctattgttgttgccgcaaatatcattgggatcttgcacttgatattgtggattcttgatcgtctt ttcttcaaatgcatttatcgtcgccttaaatacgatttgaaaagaggccctgctacggcagggatacctgagtctatgagggaagagtaccgg caggaacagcagagtgctgtagatgttgacgatggtcattttgtcaacatagaattggagta >15A-TV-M [org=Influenza A virus] [strain=A/DK/VN/15A-TV/2008 (H5N1)] acgtacgttctctctatcatcccgtcaggccccctcaaagccgagatcgcgcagaaacttgaggatgtctttgcaggaaagaacacggatct tgaggctctcatggagtggctaaagacaagaccaatcctgtcacctctgactaaagggatcttgggatttgtattcacgctcaccgtgcccag tgagcgaggactgcagcgtagacgctttgtccagaacgccctaaatggaaatggagatccaaataacatggatagggcagttaagctatat Phòng SHPT-Viện Pasteur TP.HCM 88 Báo cáo tổng kết đề tài aagaagctgaaaagagaaataacattccatggggctaaggaggtcgcactcagctactcaaccggtgcacttgccagttgcatgggtctca tatacaacaggatgggaacggtgactacagaagtggcttttggcctagtgtgtgccacttgtgagcagattgcagattcacagcatcggtctc acagacagatggcaactatcaccaacccactaatcagacatgaaaacagaatggtgctggccagcactacagctaaggctatggagcag atggcgggatcaagtgagcaggcagcggaagccatggagatcgctaatcaggctaggcagatggtgcaggcaatgaggacaattggga ctcatcctaattctagtactggtctgagagataatcttcttgaaaatttgcaggcctaccagaaacgaatgggagtgcagatgcagcgattcaa gtgatcctattgttgttgccgcaaatatcattgggatcttgcacttgatattgtggattcttgatcgtcttttcttcaaatgcatttatcgtcgccttaa atacgatttgaaaagaggccctgctacggcagggatacctgagtctatgagggaagagtaccggcaggaacagcagagtgctgtagatgt tgacgatggtca >17A-TV-M [org=Influenza A virus] [strain=A/DK/VN/17A-TV/2008 (H5N1)] aagatgagtcttctaaccgaggtcgaaacgtacgttctctctatcatcccgtcaggccccctcaaagccgagatcgcgcagaaacttgagga tgtctttgcaggaaagaacacggatcttgaggctctcatggagtggctaaagacaagaccaatcctgtcacctctgactaaagggatcttgg gatttgtattcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccagaacgccctaaatggaaatggagatccaaat aacatggatagggcagttaagctatataagaagctgaaaagagaaataacattccatggggctaaggaggtcgcactcagctactcaaccg gtgcacttgccagttgcatgggtctcatatacaacaggatgggaacggtgactacagaagtggcttttggcctagtgtgtgccacttgtgagc agattgcagattcacagcatcggtctcacagacagatggcaactatcaccaacccactaatcagacatgaaaacagaatggtgctggccag cactacagctaaggctatggagcagatggcgggatcaagtgagcaggcagcggaagccatggagatcgctaatcaggctaggcagatg gtgcaggcaatgaggacaattgggactcatcctaattctagtactggtctgagagataatcttcttgaaaatttgcaggcctaccagaaacga atgggagtgcagatgcagcgattcaagtgatcctattgttgttgccgcaaatatcattgggatcttgcacttgatattgtggattcttgatcgtctt ttcttcaaatgcatttatcgtcgccttaaatacgatttgaaaagaggccctgctacggcagggatacctgagtctatgagggaagagtaccgg caggaacagcagagtgctgtagatgttgacgatggtcatt >2B-TV-M [org=Influenza A virus] [strain=A/VN/2B-TV/2008 (H5N1)] acatgtagagagatgagtcttctaaccgaggtcgaaacgtacgttctctctatcatcccgtcaggccccctcaaagccgagatcgcgcagaa acttgaggatgtctttgcaggaaagaacacagatcttgaggctctcatggagtggctaaagacaagaccaatcctgtcacctctgactaaag ggatcttgggatttgtattcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccagaacgccctaaatggaaatgga gatccaaataacatggatagggcagttaagctatataagaagctgaaaagagaaataacattccatggggctaaggaggtcgcactcagct actcaaccggtgcacttgccagttgcatgggtctcatatacaacaggatgggaacggtgactacagaagtggcttttggcctagtgtgtgcc acttgtgagcagattgcagattcacagcatcggtctcacagacagatggcaactatcaccaacccactaatcagacatgaaaacagaatggt gctggccagcactacagctaaggctatggagcagatggcgggatcaagtgagcaggcagcggaagccatggagatcgctaatcaggct aggcagatggtgcaggcaatgaggacaattgggactcatcctaactctagtgctggtctgagagataatcttcttgaaaatttgcaggcctac cagaaacgaatgggagtgcagatgcagcgattcaagtgatcctattgttgttgccgcaaatatcattgggatcttgcacttgatattgtggattc ttgatcgtcttttcttcaaatgcatttatcgtcgccttaaatacgatttgaaaagaggccctgctacggcagggatacctgagtctatgagggaa gagtaccggcaggaacagcagagtgctgtagatgttgacgatggtcattttgtcaacatagaattggagtaaa >36ST-M [org=Influenza A virus] [strain=A/DK/VN/36ST/2008 (H5N1)] gatgagtcttctaaccgaggtcgaaacgtacgttctctctatcatcccgtcaggccccctcaaagccgagatcgcgcagaaacttgaggatg tctttgcaggaaagaacacagatcttgaggctctcatggagtggctaaagacaagaccaatcctgtcacctctgactaaagggatcttggga tttgtattcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccagaacgccctaaatggaaatggagatccaaataa catggatagggcagttaagctatataagaagctgaaaagagaaataacattccatggggctaaggaggtcgcactcagctactcaaccggt gcacttgccagttgcatgggtctcatatacaacaggatgggaacggtgactacagaagtggcttttggcctagtgtgtgccacttgtgagca gattgcagattcacagcatcggtctcacagacagatggcaactatcaccaacccactaatcagacatgaaaacagaatggtgctggccagc actacagctaaggctatggagcagatggcgggatcaagtgagcaggcagcggaagccatggagatcgctaatcaggctaggcagatgg tgcaggcaatgaggacaattgggactcatcctaactctagtgctggtctaagagataatctccttgaaaatttgcaggcctaccagaaacgaa tgggagtgcagatgcagcgattcaagtgatcctattgttgttgccgcaaatatcattgggatcttgcacttgatattgtggattcttgatcgtctttt cttcaaatgcatttatcgtcgccttaaatacgatttgaaaagaggccctgctacggcagggatacctgagtctatgagggaagagtaccggc aggaacagcagagtgctgtagatgttgacgatggtcattttgtcaacatagaattggagtaaaaacta >38ST-M [org=Influenza A virus] [strain=A/DK/VN/38ST/2008 (H5N1)] tgtagagagatgagtcttctaaccgaggtcgaaacgtacgttctctctatcatcccgtcaggccccctcaaagccgagatcgcgcagaaact tgaggatgtctttgcaggaaagaacacagatcttgaggctctcatggagtggctaaagacaagaccaatcctgtcacctctgactaaaggga tcttgggatttgtattcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccagaacgccctaaatggaaatggagat ccaaataacatggatagggcagttaagctatataagaagctgaaaagagaaataacattccatggggctaaggaggtcgcactcagctact caaccggtgcacttgccagttgcatgggtctcatatacaacaggatgggaacggtgactacagaagtggcttttggcctagtgtgtgccactt gtgagcagattgcagattcacagcatcggtctcacagacagatggcaactatcaccaacccactaatcagacatgaaaacagaatggtgct Phòng SHPT-Viện Pasteur TP.HCM 89 Báo cáo tổng kết đề taøi ggccagcactacagctaaggctatggagcagatggcgggatcaagtgagcaggcagcggaagccatggagatcgctaatcaggctagg cagatggtgcaggcaatgaggacaattgggactcatcctaactctagtgctggtctaagagataatctccttgaaaatttgcaggcctaccag aaacgaatgggagtgcagatgcagcgattcaagtgatcctattgttgttgccgcaaatatcattgggatcttgcacttgatattgtggattcttga tcgtcttttcttcaaatgcatttatcgtcgccttaaatacgatttgaaaagaggccctgctacggcagggatacctgagtctatgagggaagagt accggcaggaacagcagagtgctgtagatgttgacgatggtcattttgtctacatagaa >41ST-M [org=Influenza A virus] [strain=A/DK/VN/41ST/2008 (H5N1)] Agcaaaagcaggtagatattgaaagatgagtcttctaaccgaggtcgaaacgtacgttctctctatcatcccgtcaggccccctcaaagcc gagatcgcgcagaaacttgaggatgtctttgcaggaaagaacacagatcttgaggctctcatggagtggctaaagacaagaccaatcctgt cacctctgactaaagggatcttgggatttgtattcacgctcaccgtgcccagtgagcgaggactgcagcgtagacgctttgtccagaacgcc ctaaatggaaatggagatccaaataacatggatagggcagttaagctatataagaagctgaaaagagaaataacattccatggggctaagg aggtcgcactcagctactcaaccggtgcacttgccagttgcatgggtctcatatacaacaggatgggaacggtgactacagaagtggctttt ggcctagtgtgtgccacttgtgagcagattgcagattcacagcatcggtctcacagacagatggcaactatcaccaacccactaatcagaca tgaaaacagaatggtgctggccagcactacagctaaggctatggagcagatggcgggatcaagtgagcaggcagcggaagccatggag atcgctaatcaggctaggcagatggtgcaggcaatgaggacaattgggactcatcctaactctagtgctggtctaagagataatctccttgaa aatttgcaggcctaccagaaacgaatgggagtgcagatgcagcgattcaagtgatcctattgttgttgccgcaaatatcattgggatcttgca cttgatattgtggattcttgatcgtcttttcttcaaatgcatttatcgtcgccttaaatacgatttgaaaagaggccctgctacggcagggatacct gagtctatgagggaagagtaccggcaggaacagcagagtgctgtagatgttgacgatggtcattttgtcaacatagaattggagtaaaaaa ctaccttgtttctact GEN NS >1CM-NS [org=Influenza A virus] [strain=A/DK/Viet Nam/CM-1/2006 (H5N1)] atggattccaacactgtgtcaagctttcaggtagactgctttctttggcatgtccgcaaacgatttgcagaccaagaactgggtgatgccccat tccttgaccggcttcgccgagatcagaagtccctaagaggaagaggcaacactcttggtctggacatcraaacagctactcgcgcaggaa agcagatagtggagcggattctggaggaggagtctgataaggcacttaaaatgccggtttcacgctacctaactgacatgactctcgagga aatgtcaagggactggttcatgctcatgcccaagcagaaagtggcaggttccctttgcatcaaaatggaccaggcaataatggataaaacc atcatattgaaagcaaacttcagtgtgacttttgaccggttggaaaacctaatactacttagagctttcacagaagggggagcaatcgtggga gaaatctcaccattaccttctcttccaggacatactggtgaggatgtcaaaaatgcaattggcatcctcatcggaggacttgaatggaatgata acacagttcaagtcactgaaactatacagagattcgcttggggaagcagtgatgaggttgggagacttccactccctccaaatcagaaccg gaaaatggcgagaacaattgagtcagaagtttgaagaaataaggtggctgattgaagaagtaagacatagattgaaaattacagaaaacag cttcgaacagataacgtttatgcaagccttacaactactgcttgaagtggagcaagagataagaaccttctcgtttcagcttatttaa >2CM-NS [org=Influenza A virus] [strain=A/DK/Viet Nam/CM-2/2006 (H5N1)] atggattccaacactgtgtcaagctttcaggtagactgctttctttggcatgtccgcaaacgatttgcagaccaagaactgggtgatgccccat tccttgaccggcttcgccgagatcagaagtccctaagaggaagaggcaacactcttggtctggacatcaaaacagctactcgcgcaggaa agcagatagtggagcggattctggaagaggagtctgataaggcacttaaaatgccggtttcacgctacctaactgacatgactctcgagga aatgtcaagggactggttcatgctcatgcccaagcagaaagtggcaggttccctttgcatcaaaatggaccaggcaataatggataaaacc atcatattgaaagcaaacttcagtgtgacttttgaccggttggaaaacctaatactacttagagctttcacagaagggggagcaatcgtggga gaaatctcaccattaccttctcttccaggacatactggtgaggatgtcaaaaatgcaattggcatcctcatcggaggacttgaatggaatgata acacagttcaagtcactgaaactatacagagattcgcttggggaagcagtgatgaggttgggagacttccactccctccaaatcagaaccg gaaaatggcgagaacaattgagtcagaagtttgaagaaataaggtggctgattgaagaagtaagacatagattgaaa >MBP5NS [org=Influenza A virus] [strain=A/teal/Viet Nam/MBP5/2006 (H5N1)] atggattccaacacgataacctcgtttcaggtagattgctatctatggcacataaggaaactgctcagcatgagagacatgtgtgatgctccct ttgatgacaggctcagaagagatcaaaaagcattaaagggaagaggcagcacacttggactcgacctgagagtggctacaatggaaggg aaaaagatcgttgaggatattctgaaaagtgaaactgatgaaaatcttaaaatagccattgcctctagtcctgctccccggtatatcactgatat gagcatagaggagataagccgggaatggtatatgctgatgcctaggcagaaaataactggaggcctgatggtgagaatggatcaagccat aatggataagagaatcatcctcaaagcaaacttctcagttttattcgaccaactagaaacgttggtctctctgagggcattcacagaagatggt gctattgtggctgaaatttctcccattccctccatgccaggacattctacagaggatgtcaaaaatgcaattggaatcctcatcggtggacttga atggaatgataactcaattcgagcgtctgaaaatatacagagatttgcttggggaatccgtgatgaggrtgggrgacctccactccctccaaa acagaaatgctacatggcgagaagagttgagtcagaagtttgaagagatcagatggctcattgctgaatgcagaaatatactggcaaagac tgagaacagttttgaacaaataacgttcttacaggcattgcaactcttacttgaagttgaaagtgagataaggactttttcttttcagcttatttaa Phòng SHPT-Viện Pasteur TP.HCM 90 Báo cáo tổng kết đề tài >9DT-NS [org=Influenza A virus] [strain=A/DK/Viet Nam/DT-9/2006 (H5N1)] atggattccaacactgtgtcaagctttcaggtagactgctttctttggcatgtccgcaaacgatttgcagaccaagaactgggtgatgccccat tccttgaccggcttcgccgagatcagaagtccctaagaggaagaggcaacactcttggtctggacatcgaaacagctactcgcgccggaa agcagatagtggagcggattctggaggaggagtctgataaggcacttaaaatgccggtttcacgctacctaactgacatgactctcgagga aatgtctagggactggttcatgctcatgcccaagcagaaagtggcaggttccctttgcatcaaaatggaccaggcaataatggataaaactat catattgaaagcaaacttcagtgtgacttttgaccggttggaaaacctaatactacttagagctttcacagaagggggagcaatcgtgggaga aatctcaccattaccttctcttccaggacatactggtgaggatgtcaaaaatgcaattggcatcctcatcggaggacttgaatggaatgataac acagttcaagtcactgaaactatacagagattcgcttggggaagcagtgatgagaatgggagacttccactccctccaaaccagaaccgga aaatggcgagaacaattgagtcagaagtttgaagaaataaggtggctgattgaagaagtaagacatagattgaaaattacagaaaacagctt cgaacagataacgtttatgcaagccttacaactactgcttgaagtggagcaagagataagaaccttctcgtttcagcttatttaa >12A-LA-NS [org=Influenza A virus] [strain=A/DK/Viet Nam/12A-LA/2008 (H5N1)] atggattccaacactgtgtcaagctttcaggtagactgctttctttggcatgtccgcaaacgatttgcagaccaagaactgggtgatgccccat tccttgaccggcttcgccgagatcagaagtccctaagaggaagaggcaacactcttggtctggacatcgaaacagctactcgcgcaagaa agcagatagtggagcggattctggaggaggagtctgataaggcacttaaaatgccggtttcacgctacctaactgacatgactctcgagga aatgtcaagggactggttcatgctcatgcccaagcagaaagtggcaggttccctttgcatcaaaatggaccaggcaataatggataaaacc atcatattgaaagcaaacttcagtgtgacttttgaccggttggaaaacctaatactacttagagctttcacagaagggggagcaatcgtggga gaaatctcaccattaccttctcttccaggacatactggtgaggatgtcaaaaatgcaattggcatcctcatcggaggacttgaatggaatgata acacagttcaagtcactgaaactatacagagattcgcttggggaagcagtgatgaggttgggagacttccactccctccaaatcagaaccg gaaaatggcgagaacaattgagtcagaagtttgaagaaataaggtggctgattgaagaagtaagacatagattgaaaattacagaaaacag cttcgaacagataacgtttatgcaagccttacaactactgcttgaagtggagcaagagataagaaccttctcgtttcagcttatttaa >15A-TV-NS [org=Influenza A virus] [strain=A/DK/Viet Nam/15A-TV/2008 (H5N1)] atggattccaacactgtgtcaagctttcaggtagactgctttctttggcatgtccgcaaacgatttgcagaccaagaactgggtgatgccccat tccttgaccggcttcgccgagatcagaagtccctaagaggaagaggcaacactcttggtctggacatcgaaacagctactcgcgcaggaa agcagatagtggagcggattctggaggaggagtctgataaggcacttaaaatgccggtttcacgctacctaactgacatgactctcgagga aatgtcaagggactggttcatgctcatgcccaagcagaaagtggcaggttccctttgcatcaaaatggaccaggcaataatggataaaacc atcatattgaaagcaaacttcagtgtgacttttgaccggttggaaaacctaatactacttagagctttcacagaagggggagcaatcgtggga gaaatctcaccattaccttctcttccaggacatactggtgaggatgtcaaaaatgcaattggcatcctcatcggaggacttgaatggaatgata acacagttcaagtcactgaaactatacagagattcgcttggggaagcagtgatgaggttgggagacttccactccctccaaatcagaaccg gaaaatggcgagaacaattgagtcagaagtttgaagaaataaggtggctgattgaagaagtaagacatagattgaaaattacagaaaacag cttcgaacagataacgtttatgcaagccttacaactactgcttgaagtggagcaagagataagaaccttctcg >17A-TV-NS [org=Influenza A virus] [strain=A/DK/Viet Nam/17A-TV/2008 (H5N1)] atggattccaacactgtgtcaagctttcaggtagactgctttctttggcatgtccgcaaacgatttgcagaccaagaactgggtgatgccccat tccttgaccggcttcgccgagatcagaagtccctaagaggaagaggcaacactcttggtctggacatcgaaacagctactcgcgcaggaa agcagatagtggagcggattctggaggaggagtctgataaggcacttaaaatgccggtttcacgctacctaactgacatgactctcgagga aatgtcaagggactggttcatgctcatgcccaagcagaaagtggcaggttccctttgcatcaaaatggaccaggcaataatggataaaacc atcatattgaaagcaaacttcagtgtgacttttgaccggttggaaaacctaatactacttagagctttcacagaagggggagcaatcgtggga gaaatctcaccattaccttctcttccaggacatactggtgaggatgtcaaaaatgcaattggcatcctcatcggaggacttgaatggaatgata acacagttcaagtcactgaaactatacagagattcgcttggggaagcagtgatgaggttgggagacttccactccctccaaatcagaaccg gaaaatggcgagaacaattgagtcagaagtttgaagaaataaggtggctgattgaagaagtaagacatagattgaaaattacagaaaacag cttcgaacagataacgtttatgcaagccttacaactactgcttgaagtggagcaagagataagaacctt >2B-TV-NS [org=Influenza A virus] [strain=A/DK/Viet Nam/2B-TV/2008 (H5N1)] tcacgctacctaactgacatgactctcgaggaaatgtcaagggactggttcatgctcatgcccaagcagaaagtgacaggttccctttgcatc aaaatggaccaggcaataatggataaaaccatcatattgaaagcaaacttcagtgtgacttttgaccggttggaaaacctaatactacttaga gctttcacagaagggggagcaatcgtgggagaaatctcaccattaccttctcttccaggacatactggtgaggatgtcaaaaatgcaattgg catcctcatcggaggacttgaatggaatgataacacagttcaagtcactgaaactatacagagattcgcttggggaagcagtgatgaggttg ggagacttccactccctccaaatcagaaccggaaaatggcgagaacaattgagtcagaagtttgaagaaataaggtggctgattgaagaa gtaagacatagattgaaaattacagaaaacagcttcgaacagataacgtttatgcaagccttacaactactgcttgaagtggagcaagagata agaaccttctcgtttc >36ST-NS [org=Influenza A virus] [strain=A/DK/Viet Nam/36ST/2008 (H5N1)] gtgacaagacataatggattccaacactgtgtcaagctttcaggtagactgctttctttggcatgtccgcaaacgatttgcagaccaagaactg ggtgatgccccattccttgaccggcttcgccgagatcagaagtccctaagaggaagaggcaacactcttggtctggacatcgaaacagcta Phòng SHPT-Viện Pasteur TP.HCM 91 Báo cáo tổng kết đề tài ctcgcgcaggaaagcagatagtggagcggattctggaggaggagtctgataaggcacttaaaatgccggtttcacgctacctaactgacat gactctcgaggaaatgtcaagggactggttcatgctcatgcccaagcagaaagtggcaggttccctttgcatcaaaatggaccaggcaata atggataaaaccatcatattgaaagcaaacttcagtgtgacttttgaccggttggaaaacctaatactacttagagctttcacagaaggggga gyagtcgtgggagaaatctcaccattaccttctcttccaggacatactggtgaggatgtcaaaaatgcaattggaatcctcatcggaggactt gaatggaatgataacacagttcaagtcactgaaactatacagagattcgcttggggaagcagtgatgaggttgggagacttccactccctcc aaatcagaaccggaaaatggcgagaacaattgagtcagaagtttgaagaaataaggtggctgattgaagaagtaagacatagattgaaaat tacagaaaacagcttcgaacagataacgtttatgcaagccttacaactactgcttgaagtggagcaagagataagaaccttctcgtttcagctt atttaatgataaaaaacacccttggtttctacta >41ST-NS [org=Influenza A virus] [strain=A/DK/Viet Nam/41ST/2008 (H5N1)] aaaaacataatggattccaacactgtgtcaagctttcaggtagactgctttctttggcatgtccgcaaacgatttgcagaccaagaactgggtg atgccccattccttgaccggcttcgccgagatcagaagtccctaagaggaagaggcaacactcttggtctggacatcgaaacagctactcg cgcaggaaagcagatagtggagcggattctggaggaggagtctgataaggcacttaaaatgccggtttcacgctacctaactgacatgact ctcgaggaaatgtcaagggactggttcatgctcatgcccaagcagaaagtggcaggttccctttgcatcaaaatggaccaggcaataatgg ataaaaccatcatattgaaagcaaacttcagtgtgacttttgaccggttggaaaacctaatactacttagagctttcacagaagggggagcagt cgtgggagaaatctcaccattaccttctcttccaggacatactggtgaggatgtcaaaaatgcaattggaatcctcatcggaggacttgaatg gaatgataacacagttcaagtcactgaaactatacagagattcgcttggggaagcagtgatgaggttgggagacttccactccctccaaatc agaaccggaaaatggcgagaacaattgagtcagaagtttgaagaaataaggtggctgattgaagaagtaagacatagattgaaaattacag aaaacagcttcgaacagataacgtttatgcaagccttacaactactgcttgaagtggagcaagagataagaaccttctcgtttcagcttatttaa tgataaaaaaacacccttgttttcta Phòng SHPT-Viện Pasteur TP.HCM 92 Báo cáo tổng kết đề tài Phụ lục Sự khác biệt chủng tạo với chủng vaccine Chủng A/Viet Nam/1194/2004 Chủng vaccine (NIBRG-14) Chủng RG LPAI CCT P CCT P CCT P CCT P CAA Q CAA Q CAA Q CAA Q AGA R CGA R AGA R AGA R Cleavage Site GAG AGA AGA AGA AAA AAG E R R R K K ACG GAG Trình tự bị loại bỏ E T GAG Trình tự bị loại bỏ E GAG - ACG E T AGA R CGA R AGA R AGA R GGA G GGA G GGA G GGA G TTA L TTA L TTA L TTA L Điểm cắt Phoøng SHPT-Vieän Pasteur TP.HCM 45
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