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TIEM NANG tfC CHE TH l/C VAT CUA CAO PHAN DOAN T tf CAY SAI DAT BA THtJY ( Wedelia trilobata) Nguyen Quoc Ciictng1, Do Tan Khang1, Nguyen Van Ay1 2, Tran Thanh Men3, Pham Van Trong Tmh4, Tran Ngoc Quy[.]
TIEM NANG tfC CHE T H l/C VAT CUA CAO PHAN DOAN T tf CAY SAI DAT BA TH tJY ( Wedelia trilobata) Nguyen Quoc Ciictng1, Do Tan Khang1, Nguyen Van Ay12, Tran Thanh Men3, Pham Van Trong Tmh4, Tran Ngoc Quy4 TOM TAT Cay sai dat ba thuy (Wedelia trilobata (L.) Hitchc) diipc nhieu nghien cbu cho thay co kha nang gay be che sinh hoc tren mot so loai thUc vat cung he sinh thai Trong nghien ebu nay, tac dong be che cua cac phan doan hexane, ethyl acetate, nilbc va methanol dilcfc thb nghiem tren sb mam, sinh triidng va phat trien cua cay xa lach (Lactuca sativa L.) Ket qua cho thay cao ethyl acetate co nong tb 2,5 - 5,0 mg/mL lam giam dang ke ty le mam va cac chi tieu sinh trbdng phat trien cua hat xa lach Ket qua dinh lbpng diep luc to dbcfc xb ly bdi phan doan ethyl acetate lam giam ham liipng diep luc to a, b va caratenoid tai nong mg/mL lan lbpt la 68,89%; 57,58% va 52,63% so vbi doi chbng Ham liipng polyphenol va flavonoid cao nhat co phan doan 1Vien Nghien ebu Phat trien Cong nghe Sinh hoc, Truong Dai hoc Can Tho 2Khoa Nong nghiep, Truong Dai hoc Can Tho Khoa Khoa hoc Tu nhien, Truong Dai hoc Can Tho Khoa Phat trien Nong thon, Truong Dai hoc Can Tho 95 Tap chi Khoa hoc va Cong nghe Ndng nghiep Viet Nam - So 06(127)/2027 ethyl acetate lan ltipt la 50,62 va 55,81 mg/g Ket qua da cung cap them cd sti khoa hoc ve hien ttipng doi khang “allelopathy” gay tic che sinh trtiting phat trien thUc vat va cho thay tiem nang ting dung loai thtic vat vao san xuat che pham nong dtiOc sinh hoc dung nong nghiep Tti khoa: Cay sai dat ba thuy (Wedelia trilobata (L.) Hitchc), cao chiet, tic che sinh hoc I DAT VAN DE “Allelopathy” la mot hien ttipng doi khang sinh hoc ctia thtic vat thong qua viec cac hop chat hoa hoc (allelochemicals) co the dtipc giai phong vao dat, khong theo nhieu CO che bao gom phan htiy cac chat tti cac bo phan cua cay, tiet cac chat tti r i hoac bay hoi Weston (2005) de han che sinh trtidng, phat trien hay tham chi tieu diet loai khac song cung he sinh thai vtii no Hien ttipng thtiting ph6 bien ti nhting loai thtic vat hoang dai xam lan (Ho Le Thi va ctv., 2015) Sai dat ba thuy ( W trilobata (L.) Hitchc), thuoc ho Ctic, la mot loai thtic vat xam lan dtiOc sti dung lam cay canh va ltip phti tren mat dat (Huang et a l, 2006; Si et a l, 2013) Loai cay dtiOc tim thay bien ti nhieu vting nhiet dtii, can nhiet dtii va dac biet la vting dong bang song Ctiu Long Hien chting dtipc coi la mot van de nghiem vi tinh phan tan cao, toe phat trien nhanh va tiem nang ve hien ttipng “allelopathy” Qiang va cong tac vien (2011) cho rang loai thtic vat chtia mot ltipng Ion cac chat co hoat tinh sinh hoc nhti ent-kaurane diterpenes, sesquiterpene lactones, phenolics, flavonoids, akaloids va triterpenes co the kim ham sti phat trien cua nhieu loai thtic vat xung quanh Muc tieu cua nghien ctiu la danh gia tiem nang tic che thtic vat cua sai dat ba thuy bang viec sti dung phan doan cao chiet khao sat len chi tieu mam, sinh trtidng phat trien va ham ltipng diep luc to ctia hat xa lach tai cac nong khac Tti do, lam cO sti nghien ctiu de ting dung loai thtic vat vao san xuat che pham sinh hoc dting nong nghiep II v At l i e u v A p h i / o n g p h Ap n g h i £ n c u t ; 2.1 Vat lieu nghien ctiu Mau thtic vat dting thi nghiem: Cay sai dat ba thtiy ti giai doan dang co hoa dtipc thu tai xung quanh khuon vien Trtiting Dai hoc Can Tho Mau sau thu dtipc dinh danh dtia vao hinh thai btii ThS Phting Thi Hang, Bo mon Sinh, Khoa Sti Pham, trtiting Dai hoc Can Tho Mau dtipc rtia sach va say kho ti 50°C bang tu say va xay bang may xay gia dung Hat giong xa lach co ty le mam 96 dat 97% dtipc mua tai ctia hang hat giong ti Can Tho 2.2 Phtidng phap nghien ctiu 2.2.1 Ly trtch va p h a n doan hdp chat Sai dat ba thtiy sau dtipc xay min, cho vao binh thuy tinh ngam dam vtii lit methanol tuan Dich trich dtipc loc qua giay loc va dtipc co quay duoi dung moi bang may co quay chan khong Cao chiet tho methanol dtipc pha vtii 200 mL ntitic cat va tiep tuc dtipc tach phan doan lan ltipt vtii cac dung moi theo thti tti gom hexane, ethyl acetate va ntitic Cac phan doan dvfpc co dac va pha loang methanol dat ti cac nong cho cac phtidng phap thi nghiem tiep theo 2.2.2 K h a o sa t h am h idn gpolyphen ol tong Ham ltipng polyphenol tong dtipc thtic hien theo mo ta cua Singleton va cong tac vien (1999) co hieu chinh Lan ltipt hoa tan cac phan doan cao chiet vtii nong 100 pg/mL methanol Rut 250 pL dung dich cao chiet cho vao ong tuyp, them vao 250 pL ntitic cat va 250 pL dung dich Folin-Ciocalteu 10%, lac manh va de phan ting xay vong phtit Sau do, them vao 250 pL Na2C 10% U toi ti 40°C 30 phtit, tien hanh ghi nhan hap thu tai btitic sting 765 nm Dtiting chuan gallic acid dtipc xac dinh vtii quy trinh ttidng tti vtii day nong 20; 40; 60; 80; 100; 120 pg/mL Thi nghiem dtipc thtic hien vtii lan lap lai Ket qua dtipc the hien bang mg gallic acid (GA)/g cao chiet cxV Ham Itipng polyphenol tong: C = m Trong do: C: ham hidng polyphenol tong (mg GA/g cao chiet); c: gia tri x tit dadng chuan gallic acid (pg/mL); V: the tich cao chiet (mL); m: khoi luOng cao chiet co the tich V (g) 2.2.3 K h d o sa t ham lU dngflavonoid tong Ham ltipng flavonoid tong thtic hien theo mo ta ctia Bag va cong tac vien (2015) co hieu chinh Lan ltipt hoa tan cac phan doan cao chiet vtii nong 500 pg/mL ethanol Rtit 200 pL dung dich cao chiet cho vao ong tuyp, them vao 200 pL ntitic cat va Tap chi Khoa hoc va Cong nghe Nong nghiep Viet Nam - So 06(127)/2021 40 pL N aN 02 5% Lac deu va u phut Them tiep 40 pL A1C13 10% de phan dng phut Cuoi cung hon hop dildc them 400 pL NaOH M va 120 pL nilbc cat roi lac deu Tien hanh do hap thu bode song 510 nm Dilbng chuan quercetin dUdc xac dinh vdi quy trinh tildng til vbi day nong 20; 40; 60; 80; 100; 120 pg/mL Thi nghiem dildc thife hien vbi lan lap lai Ket qua dildc the hien bang mg quercetin (QE)/g cao chiet cxV Ham liidng flavonoid tong: F = m Trong do, F: ham Itidng flavonoid tong (mg QFJg cao chiet); c: gia tri x tii dtidng chuan vdi quercetin (pg/mL); V: the tich cao chiet (mL); m: khoi Itidng cao chiet co V (g) 2.2.4 Thii nghiem m am va sin k trtidng p h o t trien thtfc vat Kha nang dc che mam cua cac phan doan cao chiet cay sai dat ba thuy dieu kien phong thi nghiem dildc tien hanh theo phildng phap cua Ho Le Thi va cong tac vien (2015) co hieu chinh Hat xa lach dildc rba sach, ngam node cat 24 gib, vdt va u d dieu kien toi 48 gid de tao dieu kien thuan ldi cho mam Hat giong xa lach dddc khao sat tren dia Petri (50 mm) Phan doan cao chiet HEX; EA; NC va ME doqc pha loang vdi day nong 0,5; 1,0; 2,5 va 5,0 mg/mL cho vao cac d!a Petri co lot giay loc Cac hat xa lach (10 hat/dia) nOt nanh dOOc dat vao cac dia neu tren, bao kin lai bang giay paraffin va dat dieu kien 25°C Thi nghiem dOOc lap lai lan Theo doi cac chi tieu: ty le mam (bat dau ghi nhan sau thi nghiem), chieu dai re, chieu dai than, loqng tdcfi va lOdng kho cua cay xa lach (dope ghi nhan sau thi nghiem) Ham lOdng diep luc to a (C J = 12,21 x A663 2>81 x A646' Ham lOdng diep luc to b (Cb) = 20,13 x A646 5,03 x A663 Ham lOdng caratenoid tong cong CCar = (1000 x A470 - 3,27 x C - 104 x Cb)l 198 Trong do: A663, A646, A470la cac chi so OD dtidc bang may quang 20 pL dich trich chtia cac sac to quang hdp 2.2.6 Xti It so lieu So lieu thi nghiem dOdc tinh trung binh bang phan mem Excel phien ban 2013 Phan tich phOdng sai mot chieu (ANOVA) va kiem dinh Tukey d mdc y nghia 5% de so sanh cac chi tieu thu thap giOa cac nghiem thde bang chOdng trinh thong ke Minitab phien ban 16 2.3 Thdi gian va dia diem nghien cdu Nghien cdu dope thpc hien tO thang nam 2020 den thang nam 2021 tai Bo mon Sinh hoc va Bo mon Hoa hoc, Khoa Khoa hoc Til nhien, Triidng Dai hoc Can Thd III KET QUA VA THAO LUAN 3.1 Ket qua phan tach cac phan doan cao chiet Cao tho methanol cay sai dat ba thuy diidc chiet long-long vdi cac dung moi co phan ede tang dan hexane, ethyl acetate va midc thu dildc khoi liidng cao chiet d moi phan doan dildc trinh bay bang Bang Hieu suat phan tach cac phan doan cao chiet til cay sai dat ba thuy Khoi lUUng cao chiet (gram) Hieu suat ly trich (%) Hexane (HEX) 13,48 ± 2,23 11,49 Ethyl Acetate (EA) 14,13 + 2,47 12,04 Nildc (NC) 46,97 ± 6,88 40,03 Phan doan % 0c che I - (L1 ' L2) LI x 100 Trong do: I la ty le % tic che, L( la chieu dai, Itidng trung binh cua ri hoac than mam cua cay doi chting va L2Id chieu dai, Itidng trung binh cua re hoac than mam cua cay dtidc xti ly 2.2.5 X ac dinh ham liidng sac to q u a n g h d p mdu thtic vat thii nghiem SO dung 0,5 g mau la tOdi dope ly trich acetone (80%); dich trich dope mat quang cac bode song 470,663 va 646 nm Ham lOdng diep luc to a, b va caratenoid tong (mg/g lOdng tOdi) dOdc tinh thed cac cong thOc Wellburn (1994) Phan doan ntldc thu dildc 46,97 g co hieu suat cao nhat (40,03%) va phan doan hexane thu dildc 13,47 g cb hieu suat thap nhat (11,49%) Hieu suat thu hoi cao chiet tai cac phan doan phu thuoc vao phan ctlc cua cac hdp chat sinh hoc hien dien hon hdp cao chiet va phan ctlc cua dung moi dung de tach chiet Cac dung moi co phan cilc cao va trung binh nhtl methanol va nildc cho hieu suat thu hoi cao cac hdp chat co phan a le 97 Tap chi Khoa hoc va Cong nghe Nong nghiep Viet Nam -So 06(127)/2021 cao, ngddc lai cho hieu suat thu hoi thap vdi dung moi co phan cdc kem nhd hexane va ethyl acetate (Nguyen Kim Phi Phung, 2007) 3.2 Ket qua dinh lddng polyphenol va flavonoid tong cao phan doan GaofM H em Ey! acetate Nufc Phan doan 00 Dda vao phiiOng trinh dddng chuan gallic acid y = 0,08lx - 0,0662 (R2 = 0,9901) de xac dinh ham lddng polyphenol tong cac phan doan Tat ca cac phan doan cao chiet deu chda cac hop chat polyphenol diioc trinh bay d hinh CmU H om e Etylacetate Nude Phan doan (b) Hinh Ham ldcfng polyphenol va flavonoid tong co cao tho va cac cao phan doan sai dat ba thuy Ghi chu: Ham iMng polyphenol (a) va flavonoid (b) tong la gia tri trung binh cua ba Ian lap lai Gitta cac phan doan, cdcgia tri co chQ cai theo sau giong thi khac biet khong co y nghia thong ke d miicy nghia 5% (*) qua kiem dinh Tukey Phan doan co ham ldong polyphenol tong cao nhat la ethyl acetate (282,48 mg QE/g cao chiet) Cao chiet methanol co ham lilqng polyphenol tong thap nhat (71,75 mg QE/g cao chiet) va khac biet khong co y nghia thong ke vdi phan doan cao chiet hexane (202,91 mg QE/g cao chiet) (Hinh la) Phan doan diioc ly trich dung moi ethyl acetate la dung moi co phan ciic trung binh Nhflng hop chat polyphenol to nhien thich hop hoa tan cac dung moi co phan ede trung binh hon dung moi co phan cUc manh (Niicfc), va phan ede thap (Hexane) (Galanakis et a l, 2013) Ngoai ra, d cac phan doan khac van chda ham ldong polyphenol nhat dinh mot so phan td polyphenol co nhom chdc phan ede cao hoac it phan ede co the hoa tan ddoc dung moi phan ede cao nhd methanol (Nguyen Kim Phi Phung, 2007) 3.3 Ket qua dc che mam va sinh triidng thde vat cua cac phan doan cao chiet Dda vao phdcfng trinh dddng chuan quercetin (y = 0,005x - 0,0276; R2 = 0,9901) de xac dinh ham ldong flavonoid tong cac phan doan cao chiet Ket qua cho thay, tat ca cac phan doan cao chiet deu chda cac hop chat flavonoid khac biet co y nghia thong ke so vdi cao tho ddOc the hien Hinh 1(b) Phan doan co ham ldong flavonoid tong cao Hieu qua dc che hat mam cua phan doan cao chiet sai dat ba thuy ddoc xac dinh dda tren so hat mam tren tong so hat ddoc khao sat la 10 hat d cac nong khac Ket qua khao sat kha nang dc che mam, chieu dai re, chieu dai than, ldong tddi va ldong kho cua hat xa lach bang 98 nhat la ethyl acetate (823,76 mg QE/g cao chiet) va nddc co ham ldong flavonoid tong thap nhat (57,40 mg QE/g cao chiet) Co the sai dat ba thuy cung chda nhieu flavonoid it phan ede hoa tan tot ethyl acetate, nen phan doan tren cho ket qua hsun ldong flavonoid tong cao nhat so vdi cac phan doan lai Trong nhom flavonoid, ngoai cac hop chat it phan ede cung chda nhom hop chat phan cdc Chang han cung la flavonoid nhdng co the co tinh phan cdc manh hoac trung binh vao so ldOng va tinh phan cdc cua cac nhom chdc (Nguyen Kim Phi Phung, 2007) Dieu giai thich tai cac phan doan co tinh phan cdc cao va trung binh van chda ham ldcfng flavonoid tong nhat dinh (Pham Ngoc Can va ctv., 2020) Tap chi Khoa hoc va Cong nghe Ndng nghiep Viet Nam - So 06(127)72021 Bang Phan tram tic che ty le mam, chieu dai than, chieu dai re, liipng tiidi va lifdng kho cua cac phan doan cao chiet sai dat ba thuy den xa lach Ddn vi: % Uc che Nghiem thiic Nay mam Chieu dai re Chieu dai than Trong liipng tuoi Trong liipng kho Doi chiing 0,00 ± 0,00 0,00 ± 0,00 0,00 ± 0,00 0,00 ± 0,00 0,00 ± 0,00 ME-0,5 10,20 ± 10,20s 16,17 ± 2,19bc 8,88 + 2,52bc 25,23 ± 1,68s 62,16 ± 4,20s HEX-0,5 20,41 ± 20,4 l a 16,21 ± 3,86b 37,09 ± 6,60s 15,30 ± 7,81s 33,99 ± 16,50b EA-0,5 13,61 ± 25,68a 8,71 ± l,18b 33,86 ± 10,82s 13,76 ± 32,82s 33,70 ± 13,70b Nt/DC-0,5 13,61 ± 11,7a 49,34+11,62a 19,08 ± 9,15* 26,06 ± 6,19s 42,06 ± 6,58* Doi chiing 0,00 ± 0,00 0,00 ± 0,00 0,00 + 0,00 0,00 + 0,00 0,00 ± 0,00 ME-1,0 17,01 ± U a 36,47 ± 6,66a 13,18 ± 5,58b 28,64 ± 18,81s 74,00 ± 6,49s HEX-1,0 27,21 ± 31,17s 28,93 + 1,85* 52,39 ± 7,90s 25,77 ± 22,97s 42,37 ± 24,08s EA-1,0 23,81 ± 15,59s 39,48 ± 2,00s 43,66 ± 7,97s 25,92 ± 7,69s 39,27 + 16,28s Nl/CJC-1,0 27,21 ± 5,89s 58,44 ± 24,56s 27,19 ± 20,40* 34,37 ± 11,32s 55,12 ± 6,43s Doi chiing 0,00 + 0,00 0,00 ± 0,00 0,00 ±0,00 0,00 ± 0,00 0,00 ± 0,00 ME-2,5 30,61 ± 17,67* ± 15,80s 30,16 ± 4,72bc 43,54 ±24,86* 89,59 ± 2,85s HEX-2,5 40,82 ± 20,41* 60,63 ± 2,95s 60,01 ± 1,20* 35,84 ± 6,95* 49,71 ± 16,04* EA-2,5 64,63 ± 15,59s 73,78 ± 21,12s 75,25 ± 18,56s 52,64 ± 27,55s 52,11 ± 34,04* NHDC-2,5 13,61 ± 15,59b 70,69 ± 12,56s 46,90 ± 18,53* 25,86 ± 4,94* 41,58 ± l,01bc Doi chiing 0,00 + 0,00 0,00 ± 0,00 0,00 + 0,00 0,00 ± 0,00 0,00 ± 0,00 ME-5,0 61,22 + 17,67ab 68,97 + 7,01s 50,46 + 12,92b 69,79 ± 11,74* 91,15 ± 3,39s HEX-5,0 47,62 ± 5,89bc 78,25 ± 6,58s 83,24 ± 9,26ab 41,24 ± 13,94bc 66,42 ± 5,14* EA-5,0 91,84 ± 10,20a 88,60 ± 21,37s 96,47 ± 20,71s 92,37 ± 13,22s 97,21 ± 4,84s NUOC-5,0 20,41 ± 20,4 l cd 79,98 ± 25,50s 54,38 ± 11,82b 25,56 ± 28,09c 39,69 ± 25,38b Ghi chu: Nghiem thtic doi chiing la nghiem thiic khong sit dung cao chiet Cac phan doan cao chiet cay sai dat ba thuy dUOc khdo sat d nong 0,5; 1; 2,5 va mg/mL Cac ky tu khac tren ciing mot cot bieu dien sU khac biet co y nghia 5% qua kiem dinh Tukey Ket qua cho thay stf hien dien cua cac hcfp chat he che ttfng phan doan cao chiet Trong thi nghiem nay, cac phan doan cao chiet vdi nong 2,5 - mg/mL ilc che dang ke sU mam cua hat giong xa lach so vdi doi chiing O cac nong thap hon (0,5 - mg/mL), cac phan doan cao chiet co anh hifdng lie che den sii mam cua hat giong nhiing khac biet khong co y nghia thong ke Do do, hieu qua lie che se tang vdi nong phan doan cang tang Dac biet, phan doan EA tai nong mg/mL cho thay hieu qua dc che mam cao nhat vdi 91,84% Cao tho ME va phan doan HEX d nong mg/mL cho hieu qua lie che mam thap hon lan loot la 61,22% va 47,62% Ket qua lie che phu hop vdi nghien ciiu cua tac gia Macias va cong tac vien (2004), cac chat lie che co the la cac allelochemical da tac dong vao sinh tong hop protein va axit nucleic qua trinh nguyen phan, dan den sil lie che hat giong mam Thi nghiem cho thay tac dong lie che manh me cua cay sai dat ba thuy doi vdi svi phat trien cua cay xa lach Hieu qua lie che chieu dai than, chieu dai re, liiOng tiioi va liipng kho diiOc the hien cao nhat cung d nong mg/mL vdi phan doan cao chiet ethyl acetate Phan doan EA cho hieu qua lie che chieu dai re va than lan lupt la 88,60% va 96,47% Trong do, lupng tUOi va liipng kho cung cho hieu qua lie che lan loot la 92,37% va 97,21% Theo nghien ciiu cua Nie va cong tac vien (2004) cung da phat hien tac dung dc che cua cac chat chiet xuat tii la cua W trilobata co tac dung lie che sp mam va phat trien cay cua 99 Tap chi Khoa hoc va Cong nghe Nong nghiep Viet N am -So 06(127)/2021 hoa mau, co dai va mot so thpc vat khac Hieu qua dc che cung co the dvtqc giai thich theo nghien cPu cua Swati va cong tac vien (2012) cho rang co ccf che de cac chat he che sinh triiPng va phat trien thpc vat: (1) ngan chan sp hap thu nPdc qua mang te bao va (2) giai phong cac hormon he che de tri hoan sit sinh trPcfng va phat trien thvic vat hop chat allelochemical co the lam giam sU tich tu cac phan tvi theo ba each: vie che sinh tong hop diep luc, kich thich svi phan huy diep luc to, hoac ca hai qua trinh (Chang et a l, 2002; Gomes et a l, 2017) Nghien eviu chi rang qua trinh sinh tong hop diep luc to cua cay xa lach co the bi he che bdi cac hop chat phenolic hoac flavonoid dope xem 3.4 Anh hPdng cua phan doan ethyl acetate den la cac allelochemical co phan doan cao chiet sai dat ba thuy tvi lam cham qua trinh quang hop ham lppng diep luc to cua la cay xa lach va sp phat trien cua cay xa lach Theo Bui Trang Viet (2016), cac phan diep luc to va carotenoid nam tren phPc hpp anten cua he IV KET TUAN thong quang hop co vai tro thu nhan nang ltfpng Cay sai dat ba thuy la loai thpc vat hoang dai co anh sang va chuyen cho trung tarn phan ring, tai kha nang Pc che manh sp phat trien cua cay xa lach day quang nang dviqc chuyen hoa nang thong qua chuoi chuyen dien tvi quang hop Thong Trong cac cao chiet, phan doan ethyl acetate co kha thvfdng, dPdi cac tac nhan he che, sp mat cac phan nang Pc che manh nhat den ty le mam cua hat tvi diep luc to la nguyen nhan dan den sp cham phat (91,84%) cung nhP chieu dai re (80,6%), chieu dai than (96,47%), lPpng tPOi (92,37%), trien cua thvic vat Ket qua thi nghiem cho thay ham lppng kho (97,21%) va ham lPpng cac chat diep lvipng diep luc to la cua cay xa lach giam dan tang nong phan doan cao chiet ethyl acetate luc cua cay xa lach (> 50%) Qua ket qua phan tich ham lppng polyphenol va flavonoid thi phan doan (Hinh 3) cho ket qua cao nhat Nhd vay, phan doan ethyl acetate co chPa nhieu hop chat co hoat tinh Pc che ~Diep luc to a Diep lye to b —o — Carotenoid thpc vat co the tiep tuc nghien cPu Png dung 0.4S san xuat che pham diet co sinh hoc ~ •••• ■•• TAI LIEU THAM KHAO Pham Ngoc Can, Tran Gia Han, Le Ngoc Huynh Nhii va Nguyen Diic Do, 2020 Khao sat hoat tinh khang oxy hoa va khang khuan cua cao phan doan sac ky cot silica gel tif cao chiet la ca na (Elaeocarpus hygrophilus Kurz.) Tap chi Khoa hoc trUdng Dai hoc Can Thd, 56 (3B): 77-85 Hinh Anh hiidng cua phan doan cao chiet EA tif sai dat ba thuy den ham liiong diep luc to cua la cay xa lach sau thvf nghiem Nguyin Kim Phi Phung, 2007 Phtidng phap co lap cac hdp chat hvtu cd NXB Dai hoc Qu6c gia Thanh HoChiMinh: 20-25 Ho Le Thi, Lin, C.H., Smeda, R.J., Leigh, N.D., Wycoff, W.G va Fritschi, F.B., 2015 Ket qua chiet O nong mg/mL, ham liipng diep luc to a xuat va dinh danh chat doi khang co dai Ntransva b la 0,14 mg/g, thap hon nhieu so vdi nghiem cinnamoyltyraminetP giong lua OM5930 Trong Ky thhc doi chhng (0,45 va 0,33 mg/g) Thong tvi ham yeu Hoi thao Quoc gia ve Khoa hoc Cay Ian tM lvipng carotenoid of nong mg/mL (0,09 mg/g) Vien Khoa hoc Nong nghiep Viet Nam, Ha Noi: 1151-1156 thap hon 52,63% so vdi doi chhng (0,19 mg/g) Cac phan tvi diep luc to la phan cot loi cua phde hop protein - sac to nam mang quang hop va dong mot vai tro quan trong qua trinh quang hop Khi giam cac phan tvi se lam giam hieu suat quang hop Trong hien tvipng allelopathy, cac 100 Bui Trang Viet, 2016 Sinh ly thiic vat dai ciidng NXB Dai hoc Quoc gia TP.HCM: 60-70 Bag, G., Devi, P.G and Bhaigyabati, T., 2015 Assessment of total flavonoid content and antioxidant activity of methanolic rhizome extract of three Hedychium Tap chi Khoa hoc va Cong nghe Nong nghiep Viet Nam - So 06(127)/2021 species of Manipur valley International Journal of Pharmaceutical Sciences Review and Research, 30 (1): 154-159 Chang, C.C., Yang, M.H., Wen, H.M and Chern, J.C., 2002 Estimation of total flavonoid content in propolis by two complementary colorimetric methods Journal of Food and Drug Analysis, 10 (3): 178-182 growth and physiological parameters of rice In Proceedings of the 4th World Congress on Allelopathy Wagga Wagga, New South Wales, Australia, 21-26 August 2005,469-472 Qiang, Y., Du, D.-L., Chen, Y.-J and Gao, K., 2011 entKaurane Diterpenes and Further Constituents from Wedelia trilobata Helvetica, 94: 817-823 Galanakis, C.M., Goulas, V., Tsakona, S., Manganaris, Si, C., Liu, X., Wang, C., Wang, L., Dai, Z., Qi, S and Du D., 2013 Different degrees of plant invasion G A and Gekas, V., 2013 A knowledge base for the significantly affect the richness of the soil fungal recovery of natural phenols with different solvents community PLoS One, (12): e85490 International Journal o f Food Properties, 16 (2): 382-396 Singleton, V.L., Orthofer, R and Lamuela-Raventos, R.M., 1999 Analysis of total phenol and other Gomes, M.P., Garcia, Q.S., Barreto, L.C., Pimenta, oxidation substrates and antioxidants by means of L P.S., Matheus, M.T and Figueredo, C.C., 2017 Folin-Ciocalteu reagent Methods in Enzymology, 299: Allelopathy: An overview from micro-to macroscopic 152-178 organisms, from cells to environments, and the perspectives in a climate-Changing world Biologia, Swati, J., Payal, G., Navin, K., Nishant, R., Pankaj, G 72: 113-129 and Ashish, T., 2012 A comprehensive report on Huang, X., Ou, S., Tang, S., Fu, L and Wu, J., 2006 Simultaneous determination of trilobolide-6-Oisobutyrates A and B in Wedelia trilobata by gas chromatography Chinese Journal of Chromatography, 24 (5): 499-502 therapeutic potential of Elaeocarpus ganitrus Roxb (Rudraksha) Environment Conservation Journal, 13 (3): 147-150 Wellbum, A.R., 1994 The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different Macias, F.A., Galindo, J.C.G., Molinillo, J.M.G., Cutler, resolution Journal of Plant Physiology, 144 (3): H G., 2004 Allelopathy: Chemistry and mode of 307-313 action of allelochemicals CRC, 217-227 Nie, C.R., Zeng, R.S., Luo, S.M., Li, H.S., Hong, Weston, L.A., 2005 History and current trends in the use of allelopathy for weed Management HortTechnology, M Q., and Cheng, L.Q., 2004 Allelopathic potential 15 (3): 529-534 of Wedelia trilobata L.: effects on germination, Plant inhibitory potential of fractioned extracts from Wedelia trilobata Nguyen Quoc Cuong, Do Tan Khang, Nguyen Van Ay, Tran Thanh Men, Pham Van Trong Tinh, Tran Ngoc Quy Abstract Wedelia trilobata [(L.) Hitchc] has been reported by many studies to have allelopathic potential in the ecosystem In this study, the inhibitory activity of fractions including hexane (HEX), ethyl acetate (EA), aqueous (NC) and methanol (ME) was tested on the germination and growth of lettuce (Lactuca sativa L.) Among them, the ethyl acetate fraction at 2,5 - 5,0 mg/mL concentrations has significantly suppressed lettuces germination and seedling development The EA fraction had the highest herbicidal activity The chlorophyll a, chlorophyll b and carotenoid contents decreased by 68.89%, 57.58% and 52.63%, respectively at mg/mL concentration The polyphenol and flavonoid contents presented in all fractions and were found abundant in the EA fraction with 50.62 mg GEA/g and 55.81 mg RE/g extract, respectively The findings provide scientific evidence related to inhibitory effects of W trilobata that should be further studied for bio-herbicide production Keywords: Wedelia trilobata [(L.) Hitchc)], ffactioned extract, inhibitory activity Ngay nhan bai: 19/5/2021 Ngay phan bien: 06/6/2021 Ngtidi phan bien: PGS.TS Nguyen Quang Huy Ngay duyet dang: 29/6/2021 101 ... trinh bay bang Bang Hieu suat phan tach cac phan doan cao chiet til cay sai dat ba thuy Khoi lUUng cao chiet (gram) Hieu suat ly trich (% ) Hexane (HEX) 13,48 ± 2,23 11,49 Ethyl Acetate (EA) 14,13... dat ba thuy (Wedelia trilobata (L.) Hitchc), cao chiet, tic che sinh hoc I DAT VAN DE “Allelopathy” la mot hien ttipng doi khang sinh hoc ctia thtic vat thong qua viec cac hop chat hoa hoc (allelochemicals)... (* ) qua kiem dinh Tukey Phan doan co ham ldong polyphenol tong cao nhat la ethyl acetate (2 82,48 mg QE/g cao chiet) Cao chiet methanol co ham lilqng polyphenol tong thap nhat (7 1,75 mg QE/g cao