Ảnh hưởng của môi trường và điều kiện nuôi cấy đến khả năng tổng hợp iaa của một số chủng vi khuẩn nội sinh phân lập từ rễ cây sú (a egiceras corniculatum )

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Ảnh hưởng của môi trường và điều kiện nuôi cấy đến khả năng tổng hợp iaa của một số chủng vi khuẩn nội sinh phân lập từ rễ cây sú (a egiceras corniculatum )

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ANH HUONG CUA MOI TRU IN G VA DIEU KIEN NUOI CAY DEN KHA NANG TONG HOP LAA CUA MOT SO CHUNG VIKHUAN NOl SINH PHAN LAP TtX RE CAY StJ (Aegiceras corniculatum) Nguyen Van Giang1, Vu Thi Tiidi1, Vu Thi L[.]

ANH HUONG CUA MOI T R U IN G VA DIEU KIEN NUOI CAY DEN KHA NANG TONG HOP LAA CUA MOT SO CHUNG VIKHUAN NOl SINH PHAN LAP TtX RE CAY StJ (A egiceras corniculatum ) Nguyen Van Giang1, Vu Thi Tiidi1, Vu Thi Linh1, Pham Hong Hien12 TOM TAT Cac chung vi khuan hQu ich, bao gom cac chung noi sinh la nhQng chung vi khuan dupe bon vao dat se tang ciidng sinh triibng cua cay thong qua cung cap hop chat IAA, chat vat chuyen sat-siderophore, cung cap P bang each hoa tan cac hop chat phosphate kho tan dat va tic che cac mam benh Nghien cQu nham danh gia anh hiibng cua moi trifbng nuoi cay va cac nguon carbon, nitd tori kha nang tong hop IAA cua nam chung vi khuan noi sinh (RS5, RS6, RS7, RS8, RS9) mbi dQpc phan lap tii re cay su (Aegiceras corniculatum (L.) Blanco) Re cay su dope thu thap tai Lu, huyen Giao Thuy va Mb, xa Nam Dien, huyen Nghia Hiing, tinh Nam Binh Ket qua cho thay, cac chung vi khuan RS5, RS6, RS7, RS9 tong hop IAA nhieu nhat nuoi cay moi trOPng co tinh bot va NH4N 3, pH = 7, tai 30°C, sau nuoi cay Chung RS8 tong hop IAA nhieu nhat sau nuoi cay moi triiPng co bo sung NH4C1 va tinh bot tai 35°C, pH = Te bao cua hai chung RS5 va RS7 khong co kha nang di dong Te bao cua chung RS7 va RS9 thuoc gram am TO khoa: Vi khuan noi sinh, IAA, moi triiPng, dieu kien nuoi cay, cay su (Aegiceras corniculatum (L.) I DAT VAN DE Cac chung vi khuan hQu ich, bao gom cac chung noi sinh la nhdng chung vi khuan dtipc bon vao dat se tang ciibng sinh trifcfng cua cay thong qua cung cap hop chat IAA, chat vat chuyen sat-siderophore, cung cap P bang each hoa tan cac hop chat phosphate kho tan dat, he che cac mam benh (Oteino et a l, 2015) IAA giup keo dai re, tang so liipng re ben, long hut do tang cifdng kha nang hap thu dinh diidng tii moi trOdng xung quanh Hien nay, san xuat nong nghiep, ky thuat canh tac hQu co, sQ dung cac che pham bao ve thiic vat nguon goc sinh hoc va phan bon sinh hpc dang dQpc quan tarn Khai thac va Qng dung cac chung vi sinh vat hQu ich san xuat nong nghiep la lQa chon dung, thay the dan phan bon va thuoc bao ve thQc vat nguon goc hoa hpc B e san xuat che pham sinh hpc tQ cac chung vi sinh vat hQu ich can co lQpng sinh khoi lbn dQpc san xuat thong qua len men Moi chung vi sinh vat yeu cau dieu kien len men va moi trQdng thich hop Do nghien cQu dQpc trien khai nham tim dQpc dieu kien nuoi cay va nguon carbon, nito thich hop de tang cQbng kha nang tong hop IAA cua cac chung vi khuan noi sinh tQ re cay su II VAT LIEU VA PHUONG PHAP NGHIEN CUtJ 2.1 Vat lieu nghien cQu Cac chung vi khuan noi sinh da dQpc phan lap tQ re cay su thu thap tai Lu, huyen Giao Thuy va Mcr, xa Nam Dien, huyen Nghia HQng, tinh Nam Dinh 1Khoa Cong nghe sinh hoc, Hpc vien Nong nghiep Viet Nam 2Vien Khoa hpc Nong nghiep Viet Nam (VAAS) 49 Tap chiKhoa hoc va Cong nghe Nong nghiep Viet Nam - So 06(127)/2021 2.2 Phiiofng phap nghien ciiu 2.2.1 Anh htidngcua nhiet do, p H moi tritdng, thdi giati, nguon carbon vd nitd den kha nangsinh tong hdp 1AA cua cdc chung vi khudn dddc tuyen chon Viec xac dinh mot moi triidng thich hop cho cac chung vi khuan co kha nang sinh IAA diiqc thqc hien tuan to cac yeu to mot Cac yeu to diiqc khao sat bao gom thdi gian nuoi cay (2, 3, 4, ngay), pH cua moi tnfdng nuoi cay (4, 5, 6, 7, 8, 9), nhiet nuoi cay (25°C, 30°C, 35°C va 40°C), nguon carbon (tinh bot, sucrose, lactose, sobitol, glucose) va nguon nitrogen (NHtNOj, (NTL,),SO«, NH4CI, KNO 3) Nong IAA dich nuoi vi khuan diioc dinh liiqng bang thuoc thfl Salkowski (Ghckmann and Dessaux, 1995) Hut can than mL phan dich sau ly tarn dich nuoi vi khuan cho vao cac ong nghiem va bo sung mL thuoc thd Salkowski (300 mL H2S 98%, 15 mL FeCl3 0,5M) U hon hop tren tdi 30 phut de phan dng xay hoan toan, OD d blidc song \ = 530 nra Ket qua OD cua cac chung phan lap duqc thay vao phiicfng trinh thi diidng chuan y = 0,0292x + 0,0372, R2 = 0,9976, ttf suy diiqc nong IAA cua cac chung vi khuan 2.2.2 D dnh gid k h a nang chiu m an cu a cdc chung vi khudn dtidc tuyen chon Cac chung vi khuan diiqc cay ria tren moi triidng LB, co bo sung NaCl vdi cac nong lan loot la 0%, 5%, 10%, 15%, 20% Sau nuoi cay, quan sat sii sinh triidng cua cac chung vi khuan da tuyen chon 2.2.3 K hdo sa t m ot so d q c diem h o a sinh cu a cdc chung vi khudn Kha nang di dong, sinh catalase, sd dung citrate, cac phan ling Methyl-Red, Voges-Proskauer (Nguyen Lan Dung va Dinh Thi Thuy Hang, 2006) 2.3 Thdi gian va dia diem nghien ciiu Nghien ciiu diiqc thqc hien tai Phong thi nghiem Cong nghe vi sinh, Khoa Cong nghe sinh hoc, Hoc vien Nong nghiep Viet Nam va Phong thi nghiem cua Vien Bao ve Thqc vat, Vien Khoa hoc Nong nghiep Viet Nam td thang 6/2020 den thang 5/2021 Nhiet va pH la cac yeu to vat ly tac dong manh den kha nang sinh triidng va phat trien cua sinh vat, cac chung vi khuan deu co kha nang sinh IAA dai nhiet 20°C - 40°C Cac chung RS5, RS6, RS7, RS9 tong hqp IAA nhieu nhat tai 30°C (Hinh 1A), rieng chung RS8, nong IAA cao nhat (20.1 pg/mL) d 35°C Trong nghien cdu cua Nguyen Van Giang va cong tac vien (2018), chung vi khuan Bacillus sonorensis LD18 tong hqp IAA nhieu nhat tai 30°C Panigrahia va cong tac vien (2019) da chi ro 37°C la nhiet toi liu de chung vi khuan noi sinh Enterobacter cloacae MG00145 tong hqp IAA nhieu nhat (17,934 pg/mL) Tran Bao Tram va cong tac vien (2017) da tim nhiet toi liu de chung K cryocrescens tong hqp IAA cao nhat la d nhiet 30°C, vdi liiqng IAA tao 97,71 pg/mL Mohite (2013) cung ket luan nhiet toi liu de tong hqp IAA cua cac chung vi khuan thi nghiem la 30°C Moi chung vi sinh vat se co ngvfdng nhiet toi du tai chung bieu hien cac hoat dong manh nhat Vitqt qua ngiidng nhiet toi ifu, cac phan dng trao doi chat duqc xuc tac bdi cac enzyme co the bi anh hifdng nhiet qua cao hoac qua thap deu lam chdc nang cua enzyme bi suy giam, tham chi dhng han Cac chung vi khuan thi nghiem co kha nang tong hqp IAA d cac mdc pH khao sat (Hinh IB) Da so cac chung phat trien tot nhat d pH = vdi ham liiqng IAA dao dong td 26,2 pg/mL den 39,4 pg/mL, nhien vdi chung RS8, pH thich hqp de tong hqp IAA cao nhat (23,3 pg/mL) d pH = Ket qua tiicfng ring vdi ket qua nghien cdu cua Panigrahia va cong tac vien (2019) nghien cdu dac tinh sinh tong hqp IAA cua chung vi khuan noi sinh Enterobacter cloacae MG00145; chung vi khuan B sonorensis LD18 nghien cdu cua Nguyen Van Giang va cong tac vien (2018) tong hqp IAA nhieu nhat pH moi triidng nuoi khoang Co the nhan thay, pH thap han che sq phat trien cua thqc vat vi tai pH thap, dat de bi chua, lam nong mot so ion kim loai dat den mdc doc hai dat va cho cay ('Mohite, 2013) ket qua anh hiidng tdi sinh triidng cua vi sinh vat Trong nghien cdu cua Mohite (2013), cac chung vi khuan tong hqp IAA cao nhat pH mdi triidng khoang - Co 3.1 Anh hiicfng cua nhiet va pH den kha nang the khang dinh rang pH cua dat co the tac dong tdi sinh IAA nhieu qua trinh sinh hoc dien vung re cay III KET QUA VA THAO LUAN 50 Tap chi Khoa hoc va Cong nghe Ndng nghiep Viet Nam - So 06(127)/2021 ■ RS5 ■ RS6 ■ RS7 ■ RS8 ■ RS9 20*C 25'C 30’C 35 ’C 40*C pH=4 pH=5 pH=6 pH=7 pH=8 pH=9 Cac giatripH khao sat Nhiet doth! nghiem B A Hinh Anh hiidng cua nhiet (A) va pH (B) den kha nang sinh IAA cua cac chung vi khuan thi nghiem 3.2 Anh hiidng cua thdi gian nuoi cay Thdi gian nuoi cay la mot nhflng yeu to quan anh hudng den toe phat trien cung nhii hoat tinh sinh hoc cua vi khuan Cac chung vi khuan diipc nuoi moi trUdng LB long tP den Sau nuoi cay, ca bon chung RS5, RS6, RS7, RS9 deu cho ham lupng IAA cao nhat bao gom RS5 la 39,7 pg/mL, RS6 la 37,8 pg/mL, RS7 la 29,7 pg/mL, RS9 la 24,2 pg/mL Vdi chung RS8, thdi gian thich hop de sinh IAA cao nhat la sau ■ RS5 agiy vdi ham ltipng dat 27,3 pg/mL (Hinh 2) Mot so nha khoa hoc da co cong bo ve anh htidng cua thdi gian nuoi den ham liiOng IAA dope cac chung vi sinh vat tong hpp Nguyen Thi Huynh Nhii va cong tac vien (2013) thong bao cac chung vi khuan noi sinh tii cay chuoi tong hpp IAA nhieu nhat sau nuoi cay Dieu dope dp doan la sau - ngay, cac chung vi khuan dope nuoi cay chuyen sang pha can bang, tai pha so liipng vi khuan tong hpp nhieu cac hpp chat trao doi thp cap, co IAA «RS6 «RS7 «RS8 ngiy «RS9 Thai gian Hinh Anh hiidng cua thdi gian nuoi cay den kha nang sinh IAA cua cac chung vi khuan thi nghiem 3.3 Anh hiidng cua nguon carbon va nito Nam chung vi khuan noi sinh dope danh gia kha nang sinh IAA moi triidng chPa nam nguon carbon (tinh bot, lactose, glucose, saccarose, sorbitol) de danh gia sp anh hiidng cua chung den kha nang sinh IAA Khi co tinh bot moi triidng, ham liipng IAA diipc cac chung vi khuan tong hpp cao hon so vdi cac nguon C khac (Hinh 3A) nhii glucose, saccarose va sorbitol Trong thi nghiem cua Nguyen Van Giang va cong tac vien (2018), glucose la nguon carbon thich hpp hon D-sorbitol, cac nguon diidng nhii glucose, fructose va lactose khong lam tang nong IAA moi triidng nuoi chung B.sonorensis LD18 Namita va cong tac vien (2018) da bao cao rang cac chung vi khuan noi sinh Bacillus spp phan lap Vigna radiata tong hpp IAA vdi ham liipng rat khac moi trUdng nuoi cay co cac nguon carbon la glucose, mannitol va sucrose Chung Bacillus MBN3 tong hpp IAA nhieu nhat nguon 51 Tap chi Khoa hoc va Cong nghe Nong nghiep Viet Nam - So 06(127)/2021 carbon moi triidng nuoi la mannitol, vcfi chung Bacillus MJHN1 thi nguon carbon thich hop nhat la sucrose, chung Bacillus MJHN 10 Ua thich nguon carbon la diidng glucose Cac chung vi khuan diiqc nuoi moi triidng co chuta NH4NO 3, (NH4) 2S0 , NH4C1, K N 03 Sau nuoi 48h, nong IAA moi triiOng nuoi diiqc xac dinh Ket qua (Hinh 3B) cho thay nong IAA diiqc tong hop bdi cac chung RS5, RS6, RS7 va RS9 cao nhat (tiiOng hng 26,6; 24,8; 13,6 va 16,1 pg/mL) sii dung NH4N la nguon nitO Chung RS8 tong hop IAA nhieu nhat (13,6 pg/mL) diiqc nuoi moi triiOng co bo sung NH4C1 Mohite (2013) thong bao rang K N 03 la nguon nitO phu hop vcfi chung br2, br3 va mr2, NaN03 thich hop vOi chung wr2 Patil va cong tac vien (2011) thong bao chung vi khuan noi sinh Acetobacter diazotrophicus LI tong hop IAA nhieu nhat moi triidng nuoi co cao nam men va NH4C1 Nhii vay co the nhan thay cac vi khuan diiqc phan lap tii cac loai cay va cac vung khac yeu cau nguon carbon va nitO khac de tong hop IAA ■ RS5 BRS6 IR S »R S8 BRS9 ■ RS5 «R S6 BRS7 «R S8 «R S9 60 30 Tiih bot Glucose NB4CI Saccarose Cac nguon carbon thi nghiein KN03 NH4N03 Cac nguon nitorthi nghiem B Hinh Anh hxidng cua nguon cacbon (A) va nitO (B) den kha nang tong hop IAA cua cac chung vi khuan thi nghiem 3.4 Kha nang chiu man cua cac chung vi khuan Vi sinh vat noi sinh cii tru va sinh triidng, phat trien ben cay noi moi trUOng co nhieu ion khac va chiu diiqc cac nhan to sinh hoc va phi sinh hoc Nhieu tac gia da cong bo kha nang chiu diiqc nong muoi cao cua cac chung vi khuan noi sinh (Arun Karnwal, 2020; Kumar et a l, 2015) Hinh Kha nang chiu muoi cua cac chung vi khuan thi nghiem 52 Tap chi Khoa hoc va Cong nghe Nong nghiep Viet Nam - So 06(127)/2021 Vcfi dac thu sinh song chu yeu d cUa bien, ven bien cay su co kha nang sinh triidng d ca vung niidc Id va dieu kien man Vi the, he vi khuan noi sinh diidc phan lap tii cay su co kha nang thich nghi vcfi cac nong muoi khac Ket qua nghien cilu (Hinh 4) cho thay ca nam chung RS5, RS6, RS7, RS8, RS9 deu co kha nang sinh triidng vdi mUc man khoang - % NaCl Khi man tang tcfi 20% NaCl chung sinh triidng yeu hoac khong the phat trien Trong nghien cilu cua Rashid va cong tac vien (2012), chung vi khuan Pseudomonas sp chiu diidc 4% NaCl, chung Bacillus sp chi chiu diidc 2% NaCl Chung vi khuan noi sinh BoGl21 phan lap tii Bougainvillea glabra (cay hoa giay) chiu diidc nong NaCl tii - 8,5% (Arun Karnwal, 2020) Cac vi sinh vat chiu diidc nong muoi trung binh thi co the sinh triidng tai nong muoi tii - 20% (Larsen, 1986) Vi the co the xep nam chung vi khuan tuyen chon vao nhom vi sinh chiu diidc nong muoi trung binh 3.5 Khao sat mot so dac diem sinh hoc, hoa sinh cua cac chung vi khuan da tuyen chon Khao sat cac dac diem hinh thai khuan lac, cac chung diidc nhuom gram va quan sat diicfi kinh hien vi cac dac diem sinh hoa nhii kha nang di dong, hoat tinh catalase, kha nang sii dung citrate, phan ling VP va phan ling MR diidc the hien tai bang Bang Dac diem hinh thai khuan lac, te bao va mot so phan ling hoa sinh cua cac chung vi khuan diipc tuyen chon Dac diem hinh thai khuan lac, te bao Chung RS5 Dae diem Trang trong, tron, trdn, mep khuan lac nho cao Trpc khuan, gram diidng, te bao khong co kha nang di dong f ^ * i • • * Phan Ung MR, VP va citrate: + Phan Ung catalase: - j n A□ » gjgM M M M ttM M yM fe RS6 Trang sGa, mep khuan lac day Trpc khuan, gram diidng.Te bao co kha nang di dong RS7 Trang sfla, tron, vien kho Trpc khuan, gram am Te bao khong co kha nang di dong Phan Ung hoa sinh Nhuom Gram Khudn lac Phan Ung MR, VP, citrate va catalase: + Phan Ung MR, VP, citrate va catalase: + H1 RS8 RS9 Trang due, be mat trdn TrUc khuan, gram dUdng Te bao co kha nang di dong Trang trong, be mat trdn nhay, tron, khuan lac co kich thuPc ldn TrUc khuan, gram am Te bao co kha nang di dong Ghi chu: Ky hieu h ¥ A-iwy M v Phan Ung MR: -, phan Ung VP, citrate va catalase: + Phan Ung MR, VP, citrate: +; phan Ung catalase: - dUdng tinh, “ - ”: am tinh 53 Tap chi Khoa hoc va Cong nghe Nong nghiep Viet Nam - So 06(127)/2021 IV.KETLUAN Nam chung vi khuan noi sinh (RS5, RS6, RS7, RS8, RS9) diioc phan lap tif cay su deu co kha nang sinh IAA Dieu kien nuoi cay toi liu de cac chung vi khuan RS5, RS6, RS7, RS9 tong hop IAA manh nhat la moi triiOng vcfi nguon carbon, nito phu hop la tinh hot va NH4N 3, pH = 7, sau ngay, 30°C Chung RS8 tong hop IAA nhieu nhat sau nuoi cay moi triidng co bo sung NH4C1 va tinh hot tai 35°C, pH = Mau sac khuan lac cua nam chung thay doi td trang den trang stia, due Te bao cua hai chung RS5 va RS7 khong co kha nang di dong Te bao cua chung RS7 va RS9 thuoc gram am TAI LIEU THAM KHAO Nguyen Lan Dung, Dinh Ihiiy Hang, 2006 Vi sinh vat NXB Giao due Nguyen Van Giang, Tran Thi Dao, Tran Thi Thuy Ha, Nguyen Thu Trang, 2018 Anh hudng cua dieu kien nuoi cay tefi kha nang sinh tong hop Indole-3-acetic acid cua vi khuan Bacillus soronensis LD18 Tap chi Khoa hoc Cong nghe Ndng nghiep Viet Nam, 11(96): 90-95 Nguyen Thi Huynh Nhvi, Nguyen Hflu Hiep, Nguyen Minh DOi, Tran Nguyin Nhat Khoa, Thai Tran PhiiOng Minh, 2013 Phan lap cac dong vi khuan noi sinh co kha nang tong hop IAA va co dinh dam tren cay chuoi Tap chi Khoa hoc TrUdng Dai hoc Can Thd, 27:24-31 Tran Bao Tram, Nguyen Thi Hien, Pham HOOng Son, Nguyen Thi Thanh Mail, Vo Thu Giang, Pham The Hai, 2017 Phan lap va tuyen chon vi khuan sinh tong hop IAA (Indole Acetic Acid) td dat sam Viet Nam Quang Nam Tap chi Khoa hoc DHQGHN: Khoa hoc TU nhien va Cong nghe, 33(2S): 219-226 Arun Karnwal, 2020 Effect of salt stress-tolerant bacterial endophytes from Bougainvillea glabra on the growth of Triticum aestivum L var HD 2687 and Zea mays var PSCL-4642 BioTechnologia Journal of Biotechnology, Computational Biology and Bionanotechnology, 101 (2): 89-99 Glickmann E., And Y Dessaux, 1995 A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria Appl Environ Microbiol, 61(2): 793-796 Kumar V., Kumar A., Pandey K.D., Roy B.K., 2015 Isolation and characterization of bacterial endophytes from the roots of Cassia tora L Ann Microbiol, 65: 1391-1399 Larsen, H., 1986 Halophilic and halotolerant microorganism: an overview historical perspective FEMS Microbiol Biotechnol., 24:2235-2241 Mohite B, 2013 Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth Journal of Soil Science and Plant Nutrition, 13(3): so trang Namita, B., Maheshwari, R., Negi, M and Suneja, P, 2018 Optimization of IAA production by endophytic Bacillus spp from Vigna radiate for their potential use as plant growth promoters Israel Journal of Plant http://booksandjournals.brillonline.com/ Sciences, content/journals/10.1163/22238980-00001025 Oteino N., Lally R.D., Kiwanuka S., Lloyd A., Ryan D., Germaine K.J and Dowling D.N., 2015 Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates Front Microbiol 6:745 doi: 10.3389/fmicb.2015.00745 Panigrahia S., Mohantyb S & Rathe C.C, 2019 Characterization of endophytic bacteria Enterobacter cloacaeMG00145 isolated from Ocimum sanctum with Indole Acetic Acid (IAA) production and plant growth promoting capabilities against selected crops South African Journal of Botany, 7: 25 Patil, N.B., Gajbhiye, M., Sangita, S A., Aparna, B., Balasaheb, P., 2011 Optimization of Indole 3-acetic acid (IAA) production by Acetobacter diazotrophicus LI isolated from Sugarcane Int J of Env Sci., (1): 307-314 Rashid S, Charles T.C, Glick B.R, 2012 Isolation and characterization of new plant growth promoting bacterial endophyte Appl Soil Ecol, 61: 217-224 Effects of culture media and condition on IAA producing ability of endophytic bacteria isolated from roots of river mangrove (A egiceras corniculatum ) Nguyen Van Giang, Vu Thi Tuoi, Vu Thi Linh, Pham Hong Hien Abstract Useful bacteria, including endophytic bacterial strains when fertilized into the soil, will enhance crop growth by supplying IAA, iron carrier-siderophore, supplying P by dissolving insoluble phosphate compounds in the soil, inhibiting pathogens This study was carried out to investigate the effects of culture media and carbon and nitrogen sources on the IAA synthesis of five new endophytic bacterial strains (RS5, RS6, RS7, RS8, RS9) isolated from roots 54 Tap chi Khoa hoc va Cong nghe Nong nghiep Viet Nam - So 06(127)/2021 of river mangrove (Aegiceras corniculatum (L.) Blanco) The river mangrove roots were collected at Lu dune, Nam Dien commune, Nghia Hung district, Nam Dinh province The results showed that strains RS5, RS6, RS7, and RS9 synthesized maximum IAA quality when they were cultured in medium with pH = and the starch and ammonium nitrate as carbon and nitrogen sources, at 30°C, after three days of incubation The highest IAA amount was produced by the strain R8 after four days of culture in medium supplemented with NH4C1 and starch at 35°C and pH = Cells of the two strains RS5 and RS7 are not mobile Cells of the strains RS7 and RS9 belong to gram-negative Keywords: Endophytic bacteria, IAA, carbon and nitrogen sources, river mangrove (Aegiceras corniculatum L.) Ngaynhanbai: 15/5/2021 Ngay phan bien: 10/6/2021 Ngiidi phan bien: PGS TS Le Nhii Kieu Ngay duyet dang: 29/6/2021 ... hxidng cua nguon cacbon (A) va nitO (B) den kha nang tong hop IAA cua cac chung vi khuan thi nghiem 3.4 Kha nang chiu man cua cac chung vi khuan Vi sinh vat noi sinh cii tru va sinh triidng, phat... Chung vi khuan noi sinh BoGl21 phan lap tii Bougainvillea glabra (cay hoa giay) chiu diidc nong NaCl tii - 8,5% (Arun Karnwal, 202 0) Cac vi sinh vat chiu diidc nong muoi trung binh thi co the sinh. .. cong tac vien (201 8), chung vi khuan Bacillus sonorensis LD18 tong hqp IAA nhieu nhat tai 30°C Panigrahia va cong tac vien (201 9) da chi ro 37°C la nhiet toi liu de chung vi khuan noi sinh Enterobacter

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