Bước đầu nghiên cứu xử lý rơm rạ bằng trichoderma SPP làm thức ăn nuôi trùn quế

ÀNH PH TR ÊN C B TRICHODERMA SPP LÀM TH ÙN QU Chuyên ngành: CÔNG NGH Mã s 42 80 LU TP , tháng CƠNG TRÌNH ÀN THÀNH T – -HCM Cán b ÀNG D Cán b PGS.TS NGUY Cán b Cán b Lu Thành ph H PGS.TS NGUY PGS.TS NGUY TS HU TS PHAN NG Xác nh ngành sau lu CH ÒA ên ã HCM – – -oOo - : Phái: Nam Ngày, tháng : Chuyên ngành: I MSHV: 11310633 :“ U NGHIÊN C U X LÝ R TRICHODERMA SPP LÀM TH II B NG ” : - Phân l p ch ng Trichoderma có kh nhân sinh kh i b i cellulose lignin cao bán r n - u ki n nuôi c n sinh t ng h p enzyme cellulase, lignin peroxidase manganese peroxidase - nh thành ph n ch y - nh y u t ng lignin x lý r b ng dung d ch ki m - nh y u t th trình phân h - m, t l ch ph m VSV n Th nghi m ph i tr III sau x lý v i phân bị t : ni trùn qu tháng IV : tháng V : TS G PGS.TS Tp TS , ngày 24 tháng 12 PGS.TS N cs ã cH ng chuyên ngành thông qua NG KHOA (H tên ch ký) L ên, thành kính ình ã ày hơm tình h truy hoàn thành lu c ãt àt ình th ình h ày Xin chân thành c - Ban giám hi t òng th ãt ên c - Các th ãd , M òng tri ân c Trân tr , s àm ch h nơng, làm th ùng s hàng hóa d , , Trichoderma , có (Perionyx excavatus) Trichoderma spp 30 65%, , 5% 11.4% 3.67% : 80% ( ), : 20% 154.55% 60g sau 30 ngày nuôi ABSTRACT In Vietnam, rice straw after each harvest is used for many purposes: using for livestock bedding, cultivating straw mushroom, using as fuel for farmer, feeding cattle , applying electric production, padding fragile goods , transporting fruit or burning directly on field However, the treatment of rice straw, in fact, doesn’t get much efficiency and is causing environmental pollution and affecting human health To overcome these, we used the molds were Trichoderma strains which were isolated from plant soil and degrading agricultural wastes with high capability of cellulose and lignin degradation to reduce the degradation time Then, we used degraded rice straw as a food source of earthworm (Perionyx excavatus) Our purposes were to improve environmental problems and contribute to get economic efficiency for farmers in agricultural production with closed cycle Through the criterial evaluation of cellulose and lignin contents showed Trichoderma spp were the most effective degradation after 30 days cultivation , with the initial strain rate of % and 65 % humidity, the correlatively contents of cellulose and lignin decreased 11.4 % and 3.67 % from initial materials Research results also showed that in the food ratio: 80 % cow dung : 20 % rice straw (through composting), earthworm biomass increased the highest rate with growth rate is 154.55 % and the earthworm weight increased 60g after 30 days cultivation B ligninase CMC lignin 45 B khu 46 B Trichoderma 50 B Trichoderma 51 B ch Trichoderma 51 4.6: Trichoderma 53 4.7: Trichoderma54 4.8: Trichoderma 54 4.9: Trichoderma 55 4.10: Trichoderma58 4.11: Trichoderma 57 4.12: ch Trichoderma 58 4.13: Trichoderma 59 B Trichoderma 60 B ành ph 62 B 63 B 63 B lignin 64 B lignin 66 B lignin 67 B B ành ph ày 68 ùn qu 69 Hình 2.1: Hình 2.2 : Quá trình phân gi Hình 2.3: C 10 Hình 2.4: Các 11 Hình 2.5: Liên k 12 Hình 2.6: Trichoderma sp sau ngày nuôi c PGA 17 Hình 2.7: Trùn qu Perionyx excavates) 20 Hình 4.1: Hình quan sát vi th Trichoderma T5 (Hypocrea koningii) 47 Hình 4.2: Hình quan sát vi th Trichoderma T10 (Trichoderma harzianum)48 Hình 4.3: Hình quan sát vi th Trichoderma T11(Hypocrea jecorina) 49 Hình 4.4 Bi c Trichoderma 50 Hình 4.5: Bi ch Trichoderma 51 Hình 4.6: Bi ba ch Trichoderma 52 Hình 4.7: Bi Trichoderma 53 Hình 4.8: Bi Trichoderma 54 Hình 4.9: Bi Trichoderma 55 Hình 4.10: Bi Trichoderma56 Hình 4.11: Bi Trichoderma 57 Hình 4.12: Bi Trichoderma 58 S 454/2013/DVVS K THÔNG TIN V TH TR M M n Yêu c Gi ình t ình t NA tra c S ên BLAST SEARCH K SAB K ình t 28S CCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGCTCAGTGAGGCGTCC GGACTGGCCCAGAGAGGTGGGCAACTACCACTCAGGGCCGGAAAGCTCTCCAAAC TCGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGG AGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACCATACCAAACTGTT GCCTCGGCGGGGTCACGCCCCGGGTGCGTCGCAGCCCCGGAACCAGGCGCCCGCC GGAGGGACCAACCAAACTCTTTACTGTAGTCCCCTCGCGGACGTTATTTCTTACAG CTCTGAGCAAAAATTCAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTG GCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGT GAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCC TGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATC GGGAACCCCTCAAACGGGATCCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAG CCTCTCCTGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCGCGTCCACGTC CGTAAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTG AACTTAAGCATATCAATAAGCGGAGGAAAAAAAACCAACAGGGATTGCCCCAGTAA CGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCTAGGGTCCGAGTT GTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAAACGGGA CGCCACAGAGGGTGAGAGCCCCGTCTGGCTGGCCACCGAGCCTCTGTAAAGCTCC TT K ên BLAST SEARCH Hypocrea koningii 18S rRNA gene, 5.8S rRNA gene, 28S rRNA gene (partial), internal transcribed spacer (ITS1) and internal transcribed spacer (ITS2), strain ATCC 64262 Sequence ID: emb|AJ301990.1|Length: 2920Number of Matches: Re la t e d I n for m a t ion Ra n ge : t o 5 Ge n Ba n k Gr aph icsN e x t M a t ch Pr e v iou s M a t ch Align m e n t st a t ist ics for m a t ch # Scor e Ex pe ct I de n t it ie s Ga ps St r a n d bit s( 9 ) 0 944/ 951(99% ) 2/ 951(0% ) Plu s/ Plus Query CCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGCTCAGTGAGGCGTCCGGAC 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1602 CCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGCTCAGTGAGGCGTCCGGAC 1661 Query 61 TGGCCCAGAGAGGTGGGCAACTACCACTCAGGGCCGGAAAGCTCTCCAAACTCGGTCATT 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1662 TGGCCCAGAGAGGTGGGCAACTACCACTCAGGGCCGGAAAGCTCTCCAAACTCGGTCATT 1721 Query 121 TAGAGGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACC 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1722 TAGAGGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACC 1781 Query 181 GAGTTTACAACTCCCAAACCCAATGTGAACCATACCAAACTGTTGCCTCGGCGGGGTCAC 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1782 GAGTTTACAACTCCCAAACCCAATGTGAACCATACCAAACTGTTGCCTCGGCGGGGTCAC 1841 Query 241 GCCCCGGGTGCGTCGCAGCCCCGGAACCAGGCGCCCGCCGGAGGGACCAACCAAACTCTT 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1842 GCCCCGGGTGCGTCGCAGCCCCGGAACCAGGCGCCCGCCGGAGGGACCAACCAAACTCTT 1901 Query 301 TACTGTAGTCCCCTCGCGGACGTTATTTCTTACAGCTCTGAGCAAAAATTCAAAATGAAT 360 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1902 T-CTGTAGTCCCCTCGCGGACGTTATTTCTTACAGCTCTGAGCAAAAATTCAAAATGAAT 1960 Query 361 CAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCG 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1961 CAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCG 2020 Query 421 ATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCC 480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2021 ATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCC 2080 Query 481 CGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGG 540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2081 CGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGG 2140 Query 541 GGGGTCGGCGTTGGGGATCGGGAACCCCTCAAACGGGATCCCGGCCCCGAAATACAGTGG 600 ||||||||||||||||||||||||||||| | |||||||||||||||||||||||||||| Sbjct 2141 GGGGTCGGCGTTGGGGATCGGGAACCCCTAAGACGGGATCCCGGCCCCGAAATACAGTGG 2200 Query 601 CGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCG 660 |||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||| Sbjct 2201 CGGTCTCGCCGCAGCCTCTCATGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCG 2260 Query 661 CGTCCACGTCCGTAAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATAC 720 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2261 CGTCCACGTCCGTAAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATAC 2320 Query 721 CCGCTGAACTTAAGCATATCAATAAGCGGAGGaaaaaaaaCCAACAGGGATTGCCCCAGT 780 |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||| Sbjct 2321 CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGT 2380 Query 781 AACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCTAGGGTCCGAGTTGT 840 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2381 AACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCTAGGGTCCGAGTTGT 2440 Query 841 AATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAAACGGGACGCCAC 900 |||||||||||||||||||||||||||||||||||||||||||||| ||||||||||| | Sbjct 2441 AATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGG-AACGGGACGCCGC Query 901 AGAGGGTGAGAGCCCCGTCTGGCTGGCCACCGAGCCTCTGTAAAGCTCCTT 951 Sbjct 2500 ||||||||||||||||||||||||||||||||||||||||||||||||||| AGAGGGTGAGAGCCCCGTCTGGCTGGCCACCGAGCCTCTGTAAAGCTCCTT 2550 K Hypocrea koningii TP H ỊNG 2499 PHÒ NG XÉ T NGHIỆ M NK-BIOTEK 793/58 TRẦ N XUÂ N SỌAN, P TÂ N HƯNG, Q.7, TP HCM ĐT: (08) 37715818, (08) 37752252 Fax: (08) 37750583, (08) 37752250 Email: phhvan.nkbiotek@gmail.com, namkhoa.biotek@gmail.com TheoSđịnh hướ ng ISO 15189 GP số: 41G8005341 455/2013/DVV : S K THÔNG TIN V TR M M n Yêu c Gi ình t ình t NA tra c S ên BLAST SEARCH K SAB K ình t 28S GAGGTGGGCAACTACCACTCAGGGCCGGAAAGCTCTCCAAACTCGGTCATTTAGAG GAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCG AGTTTACAACTCCCAAACCCCATGTGAACGTTACCAAACTGTTGCCTCGGCGGGGA TCTCTGCCCCGGGTGTTGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAA CCAAAACTCTTATTGTATACCCCCTCGCGGGTTTTTTTATAATCTGAGCCTTCTCGG CGCCTCTCGTAGGCGTTTCGAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGT TCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATT CAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCA TGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGG GATCGGCCCTGCCTTGGCGGTGGCCGTCTCCGAAATACAGTGGCGGTCTCGCCGC AGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAGCGCGGCGCGTCCACA GCCGTTAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGC TGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGT AACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGTCCCTAGGGCCCGA GTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAACGG GACGCCACAGAGGGTGAGAGCCCCG K ên BLAST SEARCH Trichoderma harzianum strain CKP01 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence Sequence ID: gb|KC330218.1|Length: 1708Number of Matches: Re la t e d I n for m a t ion Ra n ge : t o Ge n Ba n k Gr a ph icsN e x t M a t ch Pr e v iou s M a t ch Align m e n t st a t ist ics for m a t ch # Scor e Ex pe ct I de n t it ie s Ga ps St r a n d bit s( ) 0 854/ 863(99% ) 4/ 863(0% ) Plu s/ Plus Query GAGGTGGGCAACTACCACTCAGGGCCGGAAAGCTCTCCAAACTCGGTCATTTAGAGGAAG 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct GAGGTGGGCAACTACCACTCAGGGCCGGAAAGCTCTCCAAACTCGGTCATTTAGAGGAAG 60 Query 61 TAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACA 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 61 TAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACA 120 Query 121 ACTCCCAAACCCCATGTGAACGTTACCAAACTGTTGCCTCGGCGGGGATCTCTGCCCCGG 180 |||||||||||| |||||||||||||||||||||||||||||| |||||||||||||||| Sbjct 121 ACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGC-GGGATCTCTGCCCCGG 179 Query 181 GTGTTGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCAAAACTCTTATTGTA 240 ||| ||||||||||||||||||||||||||||||||||||||||||||||||| ||||| Sbjct 180 GTG-CGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCAAAACTCTTTTTGTA 238 Query 241 TACCCCCTCGCGGGtttttttATAATCTGAGCCTTCTCGGCGCCTCTCGTAGGCGTTTCG 300 |||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||| Sbjct 239 TACCCCCTCGCGGG-TTTTTTATAATCTGAGCCTTCTCGGCGCCTCTCGTAGGCGTTTCG 297 Query 301 AAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAG 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 298 AAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAG 357 Query 361 CGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCA 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 358 CGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCA 417 Query 421 CATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAA 480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 418 CATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAA 477 Query 481 CCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTGCCTTGGC-GGTGGCCGTCTCCGAAA 539 |||||||||||||||||||||||||||||||||| |||| || ||||||||||||||||| Sbjct 478 CCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTCCCTTAGCGGGTGGCCGTCTCCGAAA 537 Query 540 TACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAG 599 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 538 TACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAG 597 Query 600 CGCGGCGCGTCCACAGCCGTTAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTA 659 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 598 CGCGGCGCGTCCACAGCCGTTAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTA 657 Query 660 GGAATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTG 719 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 658 GGAATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTG 717 Query 720 CCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGTCCCTAGGGCCC 779 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 718 CCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGTCCCTAGGGCCC 777 Query 780 GAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAACGGGA 839 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 778 GAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAACGGGA Query 840 CGCCACAGAGGGTGAGAGCCCCG 862 ||||||||||||||||||||||| Sbjct K 838 CGCCACAGAGGGTGAGAGCCCCG 860 Trichoderma harzianum TP H ỊNG 837 PHÒ NG XÉ T NGHIỆ M NK-BIOTEK 793/58 TRẦ N XUÂ N SỌAN, P TÂ N HƯNG, Q.7, TP HCM ÑT: (08) 37715818, (08) 37752252 Fax: (08) 37750583, (08) 37752250 Email: phhvan.nkbiotek@gmail.com, namkhoa.biotek@gmail.com GP soá: 41G8005341 Theo định hướ ng ISO 15189 S : K 456/2013/DVV S QU THÔNG TIN V TR M M n Yêu c Gi ình t ình t NA tra c S ên BLAST SEARCH K SAB K ình t 28S GCCCAGAGAGGTGGGCAACCACCACTCAGGGCCGGAAAGCTCTCCAAACTCGGTC ATTTAGAGGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGAT CATTACCGAGTTTACAACTCCCAAACCCCAATGTGAACGTTACCAATCTGTTGCCTC GGCGGGATTCTCTTGCCCCGGGCGCGTCGCAGCCCCGGATCCCCATGGCGCCCGC CGGAGGACCAACTCCAAACTCTTTTTTTCTCTCCGTCGCGGCTCCCGTCGCGGCTC TGTTTTATTTTTGCTCTGAGCCTTTCTCGGCGACCCTAGCGGGCGTCTCGAAAATGA ATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGA AATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCA CATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCT CGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCCTCACCGGGCCGCCCCCG AAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCA CCGGGAGCGCGGCGCGGCCACAGCCGTAAAACACCCCAAACTTCTGAAATGTTGA CCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAA AGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAAT TTGAAATCTGGCCCTTACGGGTCCGAGTTGTAATTTGTAGAGGATGCTTTTGGCAA GGCGCCGCCCGAGTTCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTCTG GCTGGCCGCCGAGCCTCTGTAAAAGCTCCTTCGACGAGTCGAGTTGTTT K ên BLAST SEARCH Hypocrea jecorina 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence Sequence ID: gb|AF510497.1|Length: 5556Number of Matches: Re la t e d I n for m a t ion Ra n ge : t o 5 Ge n Ba n k Gr aph icsN e x t M a t ch Pr e v iou s M a t ch Align m e n t st a t ist ics for m a t ch # Scor e Ex pe ct I de n t it ie s Ga ps St r a n d 6 bit s( ) 0 930/ 942(99% ) 6/ 942(0% ) Plu s/ Plus Query GCCCAGAGAGGTGGGCAACCACCACTCAGGGCCGGAAAGCTCTCCAAACTCGGTCATTTA 60 ||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||| Sbjct 1624 GCCCAGAGAGGTGGGCAACTACCACTCAGGGCCGGAAAGCTCTCCAAACTCGGTCATTTA 1683 Query 61 GAGGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGA 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1684 GAGGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGA 1743 Query 121 GTTTACAACTCCCAAACCCCAATGTGAACGTTACCAATCTGTTGCCTCGGCGGGATTCTC 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1744 GTTTACAACTCCCAAACCCCAATGTGAACGTTACCAATCTGTTGCCTCGGCGGGATTCTC 1803 Query 181 TTGCCCCGGGCGCGTCGCAGCCCCGGATCCCCATGGCGCCCGCCGGAGGACCAACTCCAA 240 ||||||||||||||||||||||||||| |||||||||||||||||||||||||||| || Sbjct 1804 -TGCCCCGGGCGCGTCGCAGCCCCGGAT-CCCATGGCGCCCGCCGGAGGACCAACTC-AA 1860 Query 241 ACTCtttttttCTCTCCGTCGCGGCTCCCGTCGCGGCTCTGTTTTATTTTTGCTCTGAGC 300 |||| ||||||||||||||||||||| ||||||||||||||||||| |||||||||||| Sbjct 1861 ACTC-TTTTTTCTCTCCGTCGCGGCTTCCGTCGCGGCTCTGTTTTACCTTTGCTCTGAGC 1919 Query 301 CTTTCTCGGCGACCCTAGCGGGCGTCTCGAAAATGAATCAAAACTTTCAACAACGGATCT 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1920 CTTTCTCGGCGACCCTAGCGGGCGTCTCGAAAATGAATCAAAACTTTCAACAACGGATCT 1979 Query 361 CTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAA 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1980 CTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAA 2039 Query 421 TTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATG 480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2040 TTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATG 2099 Query 481 CCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGC 540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2100 CCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGC 2159 Query 541 CCCTCACCGGGCCGCCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGT 600 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2160 CCCTCACCGGGCCGCCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGT 2219 Query 601 AGTTTGCACACTCGCACCGGGAGCGCGGCGCGGCCACAGCCGTAAAACACCCCAAACTTC 660 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| || Sbjct 2220 AGTTTGCACACTCGCACCGGGAGCGCGGCGCGGCCACAGCCGTAAAACACCCCAAAC-TC 2278 Query 661 TGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGCG 720 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2279 TGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGCG 2338 Query 721 GAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAA 780 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2339 GAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAA 2398 Query 781 ATTTGAAATCTGGCCCTTACGGGTCCGAGTTGTAATTTGTAGAGGATGCTTTTGGCAAGG 840 |||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||| Sbjct 2399 ATTTGAAATCTGGCCCTTTCGGGTCCGAGTTGTAATTTGTAGAGGATGCTTTTGGCAAGG 2458 Query 841 CGCCGCCCGAGTTCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTCTGGCTGGCC 900 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2459 CGCCGCCCGAGTTCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTCTGGCTGGCC Query 901 GCCGAGCCTCTGTAAAAGCTCCTTCGACGAGTCGAGTTGTTT 942 ||||||||||||| ||||||||||||||||||||||| |||| Sbjct K 2519 GCCGAGCCTCTGT-AAAGCTCCTTCGACGAGTCGAGTAGTTT 2559 Hypocrea jecorina TP H ÒNG 2518 LÝ L H tên h ên: Gi : : 86/27 Ơng Ích Khiêm Q TRÌNH 2004 - 2008: , Khoa Cơng ngh sinh h 2011 - 2013: H thu , Khoa K ên ngành Cơng ngh Q TRÌNH CƠNG TÁC 2008 – : Cơng tác t - Miliket ... ngành: I MSHV: 11310633 :“ U NGHIÊN C U X LÝ R TRICHODERMA SPP LÀM TH II B NG ” : - Phân l p ch ng Trichoderma có kh nhân sinh kh i b i cellulose lignin cao bán r n - u ki n nuôi c n sinh t ng h p... 46 B Trichoderma 50 B Trichoderma 51 B ch Trichoderma 51 4.6: Trichoderma 53 4.7: Trichoderma5 4 4.8: Trichoderma 54 4.9: Trichoderma. .. Hypocreaceae Gi Trichoderma Theo Agrios (1997), Harman (2002) h s Hypocreaceae.[ 22] Trichoderma ày Trichoderma Deuteromycetes, b s Trichoderma àn Trichoderma spp c l Trang 17 2.3 m hình thái Trichoderma