Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides pptx
Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides pptx
... Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides Torben P. Frandsen1,*, Monica M. Palcic2 and Birte ... more similar for the yeast a-glucosidase and oligo-1,6-glucosidase of glycoside of hydrolase family 13 when compared to that of glucoamylase of glycosidehydrolase family 15 or of a type II a-glucosidase ... course of hydrolysis by these three members of the retaining family 13 was con®rmed by directly monitoring isomaltose hydrolysisusing1H NMR spectroscopy. Kinetic analysis of thehydrolysis of...
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Báo cáo khoa học: Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium pptx
... XLXG and XLLG are 1, 2, 3, 4, 5, 7, 8 and 9,respectively). (B) HPLC analysis of digestion products of TXG. (C)HPLC analysis of digestion products of XXXGXXXG. (D) MALDI-TOF MS analysis of digestion ... (5¢-TTTGCGGCCGCTCATAGACACAAATTGCCGGFig. 7. MALDI-TOF MS analysis of peaks A (A) and B (B) in Fig. 6.The peaks were fractionated and analyzed by MALDI-TOF MS.Peak A included oligosaccharides of DP 16–18, and peak B includedoligosaccharides ... of the enzyme. HPLC and MALDI-TOF MS analyses of the hydrolytic products of TXG clearly indi-cated that Xgh74B hydrolyzes the glycosidic bonds of unbranched glucoseresidues, like other GH family...
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Báo cáo khoa học: Substrate recognition and ®delity of strand joining by an archaeal DNA ligase docx
... Substrate recognition and ®delity of strand joining by an archaealDNA ligaseMasaru Nakatani1,2, Satoshi Ezaki1,2, Haruyuki Atomi1,2 and Tadayuki Imanaka1,21Department of Synthetic ... 20 lM, the substrates,L-DNA(40) and R-DNA(30), were consumed almostcompletely and ligated by 0.2 lM of LigTk. The concentra-tions of L-DNA(40) and R-DNA(30) were 5 lMeach and considerably ... N AD+ by theenzyme results in release of PPi or NMN from the cofactor and formation of enzyme±adenylate through t he covalentaddition of AMP to the conserved AMP-binding site lysine of the...
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Báo cáo khoa học: Substrate positioning by His92 is important in catalysis by purple acid phosphatase docx
... backbone of His92 is stabilized via hydrogen bonds between the amido nitrogen of Asn144 and the backbone carbonyl of Asn91, and between the carb-oxylate of Asp52 and the backbone nitrogen of His92. ... the number of substrate molecules hydrolyzed per enzyme molecule per second;pKes,1 and pKes,2are for deprotonation ⁄ protonation events of agroup of the enzyme substrate complex; and pKeis ... capable of interacting withthe metal site in PAP (and, presumably, in kPP and PP1) either directly or via solvent molecules, as sug-gested by the structures of uncomplexed forms of PP1 [16] and...
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Báo cáo khoa học: Hydroperoxide reduction by thioredoxin-specific glutathione peroxidase isoenzymes of Arabidopsis thaliana potx
... for AtGPX1, -2, -5 and -6 forTrx were 13. 4 · 10)2, 11.0 · 10)2, 13. 0 · 10)2 and 13. 7 · 10)2Æs)1 and Kcat⁄ Kmvalues were 3.35 · 104,5.0 · 104, 4.19 · 104 and 5.96 · 104m)1Æs)1, ... cerevisciae (yeast GPX2) and non-SeCys type GPX from human and mouse. The three con-served Cys of these GPX isoenzymes are shown by inverted triangles. The GPX conserved regions are shown by a horizontal ... reduction of hydroperoxide utilizing Trx(Fig. 2).Kmvalues for both Trx and H2O2 of AtGPX isoen-zymes were nearly similar to those of GPXs from sev-eral plants and yeast (Tables 2 and 3)....
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... Department of Biochemistry, Biosciences Institute, University College Cork, Ireland2 Department of Biochemistry, School of Pharmacy and ABCRF, University College Cork, Ireland3 Institute of Molecular ... described in the secretion, stability and oligomerization of proteins [38,39], the bioactivi-ties of enzymes [40] and the binding affinities of ligands and receptors [41]. In many instances, ... inthe majority of cells (Fig. 5A,B) and a significant num-ber of these cells simultaneously displaying reticularlocalization (55 ± 7% of NN110 /130 AA-expressingcells, 51 ± 4% of NN130/234AA-expressing...
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Báo cáo khoa học: Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism potx
... dataobtained by mixing stoichiometric amounts of CPR and NADPH showed three up-phases at 600 nm and were anal-ysed using a triple-exponential equation yielding the three rate constants k1, k2 and ... – effects of solvent and pH identify hiddencomplexity in mechanismSibylle Brenner, Sam Hay, Andrew W. Munro and Nigel S. ScruttonManchester Interdisciplinary Biocentre and Faculty of Life Sciences, ... re-analysed using global analysis. The assignment of E1 and E2to the FMN and of E3 and E4to the FAD cofactor,respectively, corresponds to the analysis of Munro et al. [19].pHFMN FAD K298 KaE1E2E3E4[FMNhqFADox]...
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Báo cáo khoa học: Differential recognition of heat shock elements by members of the heat shock transcription factor family ppt
... protein) of YYY49 and YYT42 con-tained approximately 1 and 2 ng of hHSF2, and those of YYT50 and YYT17 contained approximately 0.01 and 0.1 ng of hHSF4, asjudged by the intensity of each band. ... (HSP42,HSP78, and KAR2), 3P-type HSE (APA1, HSP10, and SSA2), gap-type HSE (CPR6, CUP1, and HSP82), step-type HSE (FSH1, SGT2, and SSA3), and atypical HSE consisting of directly repeatingnGAAn units and ... are occupied by only mHSF4, and showed that the HSF4 bindingconsensus sequence is more ambiguous than that of HSF1 and HSF2. Two hHSF4 isoforms, hHSF4a and hHSF4b, share the same DBD and HR-A ⁄...
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Báo cáo khoa học: Structural recognition of an optimized substrate for the ephrin family of receptor tyrosine kinases pot
... of cancers; tumorigenic growth,invasiveness and angiogenic pathways are clearly and directly impacted by ephrin and EPH expression levelsin tumor cells [3,6,7]. Of the 16 EPH RTKs encoded by ... phosphoty-rosine and phosphothreonine) and asparagine, and isalso tolerant of hydrophobic residues such as leucine and isoleucine (Fig. 1 and Table S1). The positionsfollowing the substrate tyrosine ... the substrate complex, coordinates the back-bone N and O of Lys–5 (2.81, 2.65 A˚) and also sup-ports orientation of Arg823. Finally, Asn830coordinates Oe1 of Glu+1 (2.65 A˚) (Fig. 2C) and...
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Tài liệu Báo cáo khoa học: Simplified yet highly accurate enzyme kinetics for cases of low substrate concentrations ppt
... polynucleotides (such asDNA gyrase and ribosomes), where the concentrations of enzymes and binding sites are often of the sameorder of magnitude. In all of these cases, the accuracy of Michaelis–Menten kinetics ... autokinaseactivity of growth factor receptors, the differencebetween substrate and enzyme is blurred. By conse-quence, the vast separation in the concentrations of enzymes and substrates disappears. ... review the QSSA and then motivate and presentthe ZDP. We apply both of these to our prototypicalexample and discuss the similarities and differencesbetween the results yielded by each of them. Finally,we...
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