wnt b catenin signaling and tumorigenesis
Tài liệu Báo cáo khoa học: a-Defensins increase lung fibroblast proliferation and collagen synthesis via the b-catenin signaling pathway doc
... GSK 3b, total GSK 3b, active ⁄ dephosphorylated b- catenin, total b- catenin and collagen-I, and cell proliferation were determined As shown in Figs 7A ,B and 8A ,B, the incubation of lung fibroblasts ... Thr390-phosphorylated GSK 3b (GSK 3b- P-T390), total GSK 3b, active ⁄ dephosphorylated b- catenin, total b- catenin and collagen-I and cell proliferation in lung fibroblasts Lung fibroblasts HFL1 were incubated with ... dephosphorylated b- catenin, total b- catenin and collagen-I in lung fibroblasts Lung fibroblasts HFL1 were incubated with or without a-defensin-1 and a-defensin-2 (2.5 lM) in the presence and absence of...
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Báo cáo khoa học: Site-specific casein kinase 1e-dependent phosphorylation of Dishevelled modulates b-catenin signaling ppt
... regulator of the Wnt < /b> signaling pathway downstream of Wnt < /b> and upstream of Dvl, axin and b- catenin CKIe and Dvl physically interact in vivo, and CKIe phosphorylates Dvl in response to Wnt < /b> signaling [9–12] ... the Wnt< /b> b- catenin signaling pathway and Dvl is an important CKIe substrate Here, we identify specific sites that are phosphorylated on Dvl-1 by CKIe HPLC ESI and tandem MS (LC-MS ⁄ MS) and immobilized ... analyzed by SDS ⁄ PAGE Sample preparation and MS analysis SDS ⁄ PAGE gel bands of phosphorylated MBP–mDvl-1 were sliced into mm cubes and washed with 100 lL of 0.1 m ammonium bicarbonate buffer...
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báo cáo khoa học: "Compound Kushen Injection suppresses human breast cancer stem-like cells by down-regulating the canonical Wnt/b-catenin pathway" potx
... membrane Primary antibodies including anti-bcatenin (BD Bioscience, USA), anti -wnt1< /b> (ab15251, Abcam, UK), anti-CyclinD1 (ab6125, Abcam, UK), anti-cMyc (ab32, Abcam, UK) were applied, followed by ... The abnormal activation of the Wnt/< /b> b- catenin signaling pathway and subsequent upregulation of b- catenin driven downstream targets – c-Myc and CyclinD1 is associated with the development of breast ... of CKI, oxymatrine, can decrease both MCF-7 cell viability and the size of the SP (by approximately 90%) by inhibiting b- catenin, the main component of the Wnt < /b> signaling pathway, in a dose-dependent...
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Báo cáo y học: " Examination of effects of GSK3β phosphorylation, β-catenin phosphorylation, and β-catenin degradation on kinetics of Wnt signaling pathway using computational method" pdf
... Phosphorylation of beta -catenin by cyclic AMP-dependent protein kinase J Biol Chem 2006, 281:9971-9976 Huang H, He X: Wnt/< /b> [beta] -catenin signaling: new (and old) players and new insights Curr Opin Cell Biol ... http://www.tbiomed.com/content/6/1/13 Stabilizes {beta} -Catenin through Inhibition of Its Ubiquitination Mol Cell Biol 2005, 25:9063-9072 Mazieres J, He B, You L, Xu Z, Jablons DM: Wnt < /b> signaling ... PTH/cAMP/PKA Signaling Facilitates Canonical Wnt < /b> Signaling Via Inactivation of Glycogen Synthase Kinase-3 beta in Osteoblastic Saos-2 Cells J Cell Biochem 2008, 104:304-317 Taurin S, Sandbo N, Qin YM, Browning...
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Báo cáo khoa học: Glycogen synthase kinase 3b and b-catenin pathway is involved in toll-like receptor 4-mediated NADPH oxidase 1 expression in macrophages ppt
... fetal bovine serum, were obtained from Life Technologies (Grand Island, NY, USA) GSK 3b, b- catenin and b- actin antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and phospho-GSK 3b, ... the Nox1 gene being regulated by GSK 3b ⁄ b- catenin are original and lead to the possibility that GSK 3b ⁄ b- catenin may contribute to the development of atherosclerosis Materials and methods Reagents ... analyzed for b- catenin using western blotting (B) Cells were fixed and stained with anti- (b- catenin) serum and DAPI and observed using fluorescent microscopy (C) Nox1 mRNA was analyzed by RT-PCR (D–F)...
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Báo cáo sinh học: "Wnt signaling and the developmental fate of lung cells" ppsx
... developmental and pathological events is beginning to become a reality Journal of Biology 2004, References Okubo T, Hogan BLM: Hyperactive Wnt < /b> signaling changes the developmental potential of embryonic ... argue that because 9.4 Journal of Biology 2004, Volume 3, Issue 3, Article of local injury and inflammation there is local up-regulation of Wnt < /b> signaling and the activity of  -catenin, and that ... Journal of Biology 2004, Volume 3, Issue 3, Article Clayton 9.3 Behind the scenes Journal of Biology asked Brigid Hogan about the motivation for her work with Tadashi Okubo on Wnt < /b> signaling in...
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Báo cáo sinh học: "Nuclear localization is required for Dishevelled function in Wnt/ -catenin signaling" pps
... Biol 1998, 14:59-88 Bienz M, Clevers H: Linking colorectal cancer to Wnt < /b> signaling Cell 2000, 103:311-320 Gumbiner BM: Carcinogenesis: a balance between  -catenin and APC Curr Biol 1997, 7:R443-R446 ...  -catenin and Dsh constructs were assessed in stage 10 embryo lysates with anti-Flag antibodies and anti-Xdsh antibodies; -tubulin serves as a loading control Dsh and DsSNLS, but not DsNLSm, are able ... antibodies were from BioGenex (San Ramon, USA), anti-Flag M2 antibody was from Sigma and anti-CK1 antibodies were from BD Biosciences (Palo Alto, USA) Anti-Myc and anti-HA monoclonal antibodies...
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Signaling pathway inhibitor library screening reveals b catenin TCF4 as a novel telomerase regulator in cancer cell lines
... the Wnt < /b> pathway either by Wnt < /b> ligand (Wnt-< /b> 3a) or LiCl (activates VII Wnt < /b> signaling by inhibiting GSK-3β) treatment as well as overexpression of a constitutively active form of β -catenin (Δ-N β -catenin) ... apoptosis induced by both 4625 (Bcl-2/Bcl-xL bispecific antisense oligonucleotide) and HA14-1 (Bcl-2 inhibitor) Overexpression of wild-type and telomerase inactive hTERT mutant blocked Bcl2-dependent ... remodeling ability and was shown to be able to loop, bend, and pair telomeric repeat arrays Its ability to modify DNA could be enhanced in the presence of TIN2, implying that both proteins have the ability...
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Regulation of chondrocyte differentiation potential involvement of wnt ß catenin signaling
... Abbott and Holtzer 1966; Von der Mark 1986), a loss of the differentiated phenotype Cultured chondrocytes tend to dedifferentiate into fibroblasts (Thirion and Berenbaum 2004) or become fibroblastic ... Antagonists inhibit extracellular signaling molecules by acting as binding proteins that block signaling proteins’ ability to bind surface receptors and thus inhibit the mediated response (Yoon and Fisher ... PBS, cells were permeabilised using 0.2% (v/v) Triton X-100 (Biorad, Hercules,CA) in PBS 2% bovine serum albumin (BSA) diluted in PBS was used as the diluent for subsequent reagents and antibodies...
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Báo cáo y học: "Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) Dual Infection"
... suggested that anti-HCV positive, anti-HBc-positive patients who lack both HBsAg and antiHBs might be a group of patients with a multiple HBV and HCV infection HBV DNA by PCR was detected in 40.8% of ... exists References 10 11 12 13 14 Table Coinfection with HBV and HCV and risk of HCC Variables Case number HCC case No HBV(-)HCV(-) HBV(+)HCV(-) HBV(-)HCV(+) HBV(+)HCV(+) 118 184 21 11.2 42.9 42.8 ... M, Wu D Study of superinfection of HBV and HCV Zhonghua Liu Xing Bing Xue Za Zhi 1999 ;20:141-143 Zarski JP, Bohn B, Bastie A, Pawlotsky JM, Baud M, Bost-Bezeaux F, Tran van Nhieu J, et al Characteristics...
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Lab B: Creating Referrals and Replication Agreements
... completed Exercise before proceeding Right-click nwtraders, and then click Insert b In the Insert Object Under dialog box, in the Relative Name box, type partner_dom Under Type of Object To Create, ... Insert Object Under dialog box a In the directory pane, expand nwtraders, and then click partner_dom b In the control pane, click Administration c In the Entry Administration dialog box, on the ... system tray b If the MMS Server icon is not present in the system tray, then perform steps b, c and d c At a command prompt, type net stop "MMS Server" and then press ENTER d At a command prompt,...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx
... includes a DNA-binding domain, a ligand-binding domain and a transactivation domain The DNA-binding domain is responsible for DNA binding specificity and dimerization, and the ligand-binding domain ... (2001) MLL and CREB bind cooperatively to the nuclear coactivator CREB-binding protein Mol Cell Biol 21, 2249–2258 Rozenblatt-Rosen O, Rozovskaia T, Burakov D, Sedkov Y, Tillib S, Blechman J, ... gene regulation and mixed lineage leukemia Acknowledgements We thank Imran Hussain, Sahba Kasiri, Bishakha Shrestha, Saoni Mandal and other Mandal lab members for useful discussion and critical...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf
... signal transduction pathways activated by stress and inflammation Physiol Rev 81, 807–869 Pearson G, Robinson F, Beers GibsonT, Xu BE, Karandikar M, Berman K & Cobb MH (2001) Mitogen-activated protein ... damaged cells and to remove cells during normal growth and development [27,40] Cells that undergo apoptosis initially become rounded, which is accompanied or followed by membrane blebbing, resulting ... reversible binding and localization to membranes, stabilize the conformation of proteins and regulate protein FEBS Journal 277 (2010) 2463–2473 ª 2010 The Authors Journal compilation ª 2010 FEBS...
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Tài liệu Báo cáo khoa học: What MAN1 does to the Smads TGFb/BMP signaling and the nuclear envelope Luiza Bengtsson pdf
... beneficial, but not necessary for MAN1 functions in TGFb ⁄ BMP signaling [14] Taken together the data suggests a role for MAN1 similar to that of the inhibitory Smad Smad inhibits TGFb ⁄ BMP signaling ... TGFb ⁄ BMP signaling by binding R-Smads? As an INM protein and not a part FEBS Journal 274 (2007) 1374–1382 ª 2007 The Author Journal compilation ª 2007 FEBS 1377 What MAN1 does to the Smads L Bengtsson ... Smads can be either activated or inhibited by phosphorylation, sumoylation and ubiquitination (reviewed in [48–54]) MAN1 antagonizes TGFb/BMP signaling by binding R-Smads Xenopus MAN1 was identified...
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Báo cáo khoa học: Docosahexaenoic acid stabilizes soluble amyloid-b protofibrils and sustains amyloid-b-induced neurotoxicity in vitro ppt
... of Ab42wt monomer oligomerization (Fig 4A) and increase in protofibril 992 Fig PUFAs not stabilize Ab42Arc protofibrils, and DHA actually accelerates Ab42Arc protofibril fibrillization into fibrils ... ª 2007 FEBS A.-S Johansson et al Docosahexaenoic acid stabilizes Ab protofibrils Fig SDS ⁄ PAGE analysis confirms DHA stabilization of soluble Ab42wt Ab42wt and Ab42Arc (50 lM) were incubated overnight ... reduce amyloid burden but not soluble Ab in Tg2576 mice [9], indicating a shift from insoluble Ab to soluble species The present study demonstrates that DHA and AA stabilize soluble protofibrils, thus...
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Báo cáo khoa học: Proteomic identification of all plastid-specific ribosomal proteins in higher plant chloroplast 30S ribosomal subunit PSRP-2 (U1A-type domains), PSRP-3a/b (ycf65 homologue) and PSRP-4 (Thx homologue) doc
... by about 30 amino acids [39] The RBD folds into a compact structure of four antiparallel b- sheets and two a-helices (b1 -a1 -b2 b3-a2 -b4 ) with the conserved RNP1 and RNP2 located in the b1 and b3 ... Isoelectric pointa 6.2 26 80 5b Ref.c [21] New New 21 > 13 13 66 5b 794a (a) 794a (b) 17 4b 5.0 < 4.9 (a) 4.9 (b) 11.8 7 25 5b (a) 06 6b (b) 63 8b (c) 387a 11.5 (a) 12.2 (b) 12.2 (c) 10.6 Calculated ... was labeled with 32P as described by the Ó FEBS 2003 supplier of Random Primed DNA Labeling Kit (Boehringer Mannheim) The lambda library (150 000 pfu) was plated on four 132-mm plates, and the...
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Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution by Spaska Angelova Stanilova pot
... Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution http://dx.doi.org/10.5772/45733 Edited by Spaska Angelova Stanilova Contributors Hiroshi Tanaka, Ekua Weba Brenu, ... Autoimmunity - Intracellular Signaling and Microbiome Contribution, Edited by Spaska Angelova Stanilova p cm ISBN 978-953-51-1028-6 free online editions of InTech Books and Journals can be found at www.intechopen.com ... estrogen receptor beta (ESR2) and a promoter variant of a gene encoding nuclear factor-kappaB (NF- B) [19, 59] To 10 Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution examine...
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Searchlights on Health by B. G. Jefferis and J. L. Nichols pptx
... Often Fatal.—Begin well and the habit of doing well will become quite as easy as the habit of doing badly "Well begun is half ended," says the proverb: "and a good beginning is half the battle." ... great crying demand of the age 2 Criticism.—This work, though plain and to some extent startling, is chaste, practical and to the point, and will be a boon and a blessing to thousands who consult ... important subjects A Consistent Consideration.—Let the reader of this work study its pages carefully and be able to give safe counsel and advice to others, and remember that purity of purpose and purity...
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Searchlights on Health: Light on Dark Corners, by B.G. Jefferis and J. L. Nichols doc
... Often Fatal.—Begin well, and the habit of doing well will become quite as easy as the habit of doing badly "Well begun is half ended," says the proverb; "and a good beginning is half the battle." ... purity of heart and mind become habitual, and the character is built up in chastity, virtue, and temperance The Best Support.—The best support of character will always be found in habit, which, according ... chaste, practical and to the point, and will be a boon and a blessing to thousands who consult its pages The world is full of ignorance, and the ignorant will always criticise, because they live...
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