transcription polymerase chain reaction based assessment of the candidate biomarkers for tongue cancer me
Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
... yratnemelpmoc sti ot sezidirbyh eborp eht ,RCP gniruD )ynamreG ,ehcoR( metsys CL eht rednu demrofrep erew noitceted tcudorp dna noitacifilpmA elcyc hcae rof ces/C 02 fo pmar eht htiw nim rof C ... elbaliava gnisu dengised erew stegrat lla rof seborp dna sremirp RCP-TR* emaN ecneuqeS eborP esreveR drawroF eborP esreveR drawroF eborP esreveR drawroF '3-pxGCCAATTTCAGCCCAGGCACAAAm-'5 '3-TYCGGTTYGGGACCATGTT-'5 ... htiw nim rof C 06 ta pets noisnetxe/gnilaenna dna ces rof C 49 ta pets noitarutaned fo selcyc 55 yb dewollof ,nim 51 rof C 49 dna nim 02 rof C 05 ta noitabucni na fo elcyc eno fo gnitsisnoc erudecorp...
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Báo cáo khoa học: "TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus" pot
... and 5' NTR didn’t Therefore, primers and probe of 3' NTR for the diagnostic purpose of JEV were selected The concentration of primers and probe giving the highest fluorescent and the lowest threshold ... respectively In the sensitivity of detection, real-time RT-PCR assay with single tube was 10-fold more sensitive than RT-PCR assay When the same person performed the real-time RT-PCR three times at the different ... in a micro reaction tube (Corbett Research, Australia) The reaction mixture for each one tube TaqMan reaction mix consisted of µl of Universal 5X buffer, µl of 10 mM dNTP, µl of enzyme mix (Qiagen,...
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Báo cáo y học: "Bench-to-bedside review: The promise of rapid infection diagnosis during sepsis using polymerase chain reaction-based pathogen detection" pptx
... to improving time-critical diagnostics, are being investigated Polymerase chain reaction approaches to the diagnosis of pathogenaemia Molecular methods based on polymerase chain reaction (PCR) ... studies evaluating the use of SepsiTest™ in a clinical setting Key issues in the application of polymerase chain reaction to the diagnosis and management of sepsis The application of PCR techniques ... oligonucleotide primers, short pieces of synthesised DNA that bind to either end of the sequence and form the starting point for DNA replication by DNA polymerase For bacterial pathogens, two basic...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... purified, the free ends of the plasmid were then shortened using Bal31 nuclease The ends were filled using the Klenow fragment of DNA Polymerase, and then religated The competitor template for glyceraldehyde ... 0.25 µg of each primer, mM MgC12 in each CD4 reaction and mM MgC12 for CD8 reactions, mM total dNTP's, and 0.5 U of Taq Polymerase For CD4 and CD8 reaction conditions were : 94° for mins, then 33 ... DNA on the y-axis Solving for the antilog of x when y = will give the concentration of unknown amount of DNA – that of CD4, CD8 or GAPDH The success of the RNA extraction and reverse transcription...
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Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm a h5n1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
... (2009), Interim guidance on the planning for the use of surgical masks and respirators in health care settings during an influenza pandemic 13 Chan PK (2002), “Outbreak of avian influenza A (H5N1) ... (2008), The development and genetic diversity of H5N1 influenza virus in China, 1996-2006”, Virology, 380, pp 243-54 23 Gambaryan A, Tuzikov A, Pazynina G, et al (2006), “Evolution of the receptor ... “Evolution of the receptor binding phenotype of influenza A (H5) viruses”, Virology; 344:432-438 24 Greninger A (2004), The Definition and Measurement of Dangerous Research 25 Guan Y, et al (2004),...
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Báo cáo lâm nghiệp: "Growing stock-based assessment of the carbon stock in the Belgian forest biomass" ppsx
... contribute to the improvement of the forest carbon stock assessment for Belgium, as also mentioned by Van Camp et al [51] rious and time-consuming activity Therefore, the establishment of new BEF ... using both formula (1) (with BEF1 and BEF2) and formula (2) (with BEF3), and this for the minimum, median and maximum BEFs s.l The ratio of the result of formula (1) to the result of formula (2), ... stocks gave the total carbon stock in the productive forests of Belgium for the year 2000, also presented in Table V Based on the use of median expansion factors for all species, the total carbon...
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Xây dựng qui trình phát hiện đồng thời yeallow head virus(YHV), taura syndrome virus(TSV) và gill associated virus(GAV) trên tôm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
... chép DNA polymerase Enzyme Ngày có nhiều polymerase chịu nhiệt khác đưa thị trường với nhiều chức chuyên biệt hay hoàn thiện Tùy mục đích phản ứng PCR mà sử dụng enzyme cho phù hợp Taq polymerase ... Dimethylsulfoxide dATP: Deoxy adenosine triphosphate dTTP: Thymedine triphosphate dCTP: Deoxycytidine triphosphate UTRs: Untranslated regions RT-PCR: Reverse transcription polymerase chain reaction ... đồng thời Yeallow head virus(YHV), Taura syndrome virus(TSV) Gill-associated virus(GAV) tôm kỹ thuật Multiplex Reverse transcription polymerase chain reaction thực nhằm mục đích 11 CHƢƠNG 1: TỔNG...
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Phát hiện và phân biệt chủng virus gây hội chứng rối loạn sinh sản và hô hấp ở lợn bằng phương pháp multiplex RT PCR (reverse transcription polymerase chain reaction)
... mẫu Polymerase Chain Reaction Phản ứng chuỗi trùng hợp Reverse Transcription Polymerase Chain Reaction Phản ứng chuỗi đồng phân hóa chép ngƣợc mRTPCR Multiplex Reverse Transcription Polymerase Chain ... CHỨNG RỐI LOẠN SINH SẢN VÀ HÔ HẤP Ở LỢN BẰNG PHƢƠNG PHÁP MULTIPLEX RT-PCR (REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION) Chuyên ngành : Động Vật Học Mã số : 60 42 01 03 LUẬN VĂN THẠC SĨ SINH ... gây hội chứng rối loạn sinh sản hô hấp lợn phƣơng pháp multiplex RT-PCR (Reverse Transcription Polymerase Chain Reaction) ” Mục tiêu: Giải trình tự toàn vùng gen ORF7 virus PRRS phân lập Việt Nam...
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Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm AH5N1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
... trình phát nhanh virus cúm gia cầm A/H5N1 mẫu bệnh phẩm kỹ thuật Multiplex Reverse Transcription Polymerase Chain Reaction Labo Trường Đại học Y Thái Bình với mục tiêu: Thiết kế lựa chọn mồi phù ... protein virus (tùy theo từng chủng virus mà có thể có hoặc không có protein PB1-F2) Bên hạt virus, phân đoạn liên kết với nucleoprotein (NP) tiểu đơn vị enzyme polymerase (bao gồm PB1, ... gắn men (EIA: Enzyme-linhked Immunosorbent Assay): Kỹ thuật sử dụng kháng thể đặc hiệu gắn enzyme để phát kháng nguyên virus Mẫu bệnh phẩm gắn lên thành giếng, sau ủ với kháng thể có gắn enzyme...
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Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm AH5N1 trong mẫu bệnh phẩm bằng kỹ thuật Multiplex Reverse Transcription Polymerase Chain Reaction (luận văn thạc sĩ)
... media M: Matrix protein NA (N): Neuraminidase NP: Nucleoprotein NS: Non-structural protein PA: Polymerase A protein PB: Polymerase B protein RT-PCR: Reverse Transcription - Polymerase Chain Reaction ... thuật RT-PCR (Reverse Transcription - Polymerase Chain Reaction) Đây k thuật sinh học phân tử, cho phép phát virus với độ xác cao, thời gian làm xét nghiệm khoảng 4-6 thực Realtime RT-PCR 12-24 thực ... trình phát nhanh virus cúm gia cầm A/H5N1 mẫu bệnh phẩm kỹ thuật Multiplex Reverse Transcription Polymerase Chain Reaction Labo Trường Đại học Y Thái Bình với mục tiêu: Thiết kế lựa chọn m i phù...
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Báo cáo khoa học: " An assessment of the RIFLE criteria for acute renal failure in critically ill HIV-infected patients" docx
... renal failure - definition, outcome measures, animal models, fluid therapy and information technology needs: the Second International Consensus Conference of the Acute Dialysis Quality Initiative ... D, Bates S, Ronco C: An assessment of the RIFLE criteria for acute renal failure in hospitalized patients Crit Care Med 2006, 34:1913-1917 Page of (page number not for citation purposes) ... approval of the version to be published Competing interests The authors declare that they have no competing interests References Bellomo R, Ronco C, Kellum JA, Mehta, RL, Palevsky P and the ADQI...
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Proteomics based identification of potential protein biomarkers for epithelial ovarian cancer
... intracystic biomarkers in the clinical setting The search for protein biomarkers of ovarian cancer has for many years focused primarily on serum biomarkers for the early diagnosis of the cancer It ... serve as molecular signposts of the pathophysiologic state of a cancer cell, as well as for the diagnosis, prognostication and assessment of therapeutic response of the cancers 16 Figure 1.2 Molecular ... endometrioid and clear cell tumours The malignant tumours usually have the similar histologic architecture of the endocervix for mucinous cancer, the endometrium for endometrioid cancer and the...
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Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx
... while one (1.3%) of them was positive for the virus by pp65 antigenemia assay The exact source of HCMV detected in the three recipients of sub-group I could assumed to be the donor as they were negative ... prepared from ml of EDTA anticoagulated blood within six hours of receipt of the specimen Smears were fixed in methanol for 10 minutes Immunofluorescence staining was carried out on the smears using ... patients for the study and provided all the clinical data IM carried out PP65 assay YSM carried out the real time PCR assay JM participated in the design of the study and performed the statistical...
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A COMPARATIVE STUDY OF THE DIAGNOSIS OF PULMONARY TUBERCULOSIS USING CONVENTIONAL TOOLS AND POLYMERASE CHAIN REACTION* pot
... performed in a 50µl reaction mix containing 0.25nmoles of forward and reverse primers, 2mM final concentration of dNTP, 1.5 U of Taq polymerase in 1X buffer and proteinase K digested sample The ... attention The present study demonstrates the utility and limitations of PCR Among the total of 144 specimens studied, the sensitivity of smear, culture and PCR was 51%, 68% and 91.5% respectively Smear ... order to validate these results In this context, a recent study examined the cost-effectiveness of polymerase chain reaction versus Ziehl-Neelsen smear microscopy for diagnosis of tuberculosis...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
... When the target messages were detected in both the control samples and the OA or RA samples by RT-PCR, the ratio for the amount of the message expressed in control samples to the amount of the message ... levels of the target messages were calculated as follows: the ∆CT of each target message was obtained by subtracting the CT of β-actin message from the CT of each target message in the same RNA ... all the members of the Department of Orthopaedic Surgery, The Jikei University School of Medicine, for the collection of samples 23 24 References R519 Saari H, Kontinen YT: Determination of the...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... instructions cDNA synthesis reaction was performed by polymerase chain reaction as described elsewhere using primer Moloney murine leukemia virus reverse transcriptase (M-MLV RT)[6-10] The first-round ... first-round PCR was performed with primers P1 and P4 A nested PCR was performed in a total volume of 25 μL containing the firstround PCR products diluted tenfold as well as each of primers P2/P4, P3/P4, ... The amplified products were cloned and sequenced, and the sequences were aligned with the H genes of a number of CDV strains available in GenBank using the MegAlign function of the DNAStar software...
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Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
... rate due to the lack of proofreading ability of the RNA-dependent RNA polymerase, which is responsible for the replication of the viral genome Published sequence data indicate that the 5' untranslated ... we found that the sets of primers/ Figure Comparison of the duplex primer/probe and singleplex primer/probe assays The performances of the duplex primer/probe (C) and singleplex primer/probe (A, ... realtime polymerase chain reaction- based method Hepatology 2007, 46:22-31 Chevaliez S, Bouvier-Alias M, Castéra L, Pawlotsky JM: The Cobas AmpliPrep-Cobas TaqMan real-time polymerase chain reaction...
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Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx
... established the applicability of real-time PCR for the quantification of GPV because of its remarkable sensitivity and high-throughput potential, which is beyond the scope of other diagnostic methods The ... 1000-times more sensitive than the conventional PCR method; the former method can detect pVP3 copies down to dilutions of 2.8 × 101 copies /reaction and the latter one that can detect copies up to the ... fluorescence was measured at the end of the annealing/extension step The tests were performed using 0.2-mL PCR tubes (ABgene, UK) FQ-PCR reactions were optimized in triplicate based on the primer, probe,...
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Báo cáo khoa học: " An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction" pptx
... of dengue cases 2003–2007 Percentage of dengue cases 2003–2007 Discussion The lack of a vaccine or a cure for dengue fever makes the development of laboratory -based surveillance systems all the ... performed according to manufacturer's instructions Briefly, RNA was extracted from 280 μl of serum using the QIAamp Viral RNA minikit Reverse transcription and polymerase chain reaction was performed ... enzyme linked immunosorbent assays (ELISA) with a single serum sample does not provide information on the serotype of the virus However, single-step reverse transcriptase polymerase chain reaction...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25