transcription factors involved in environmental stress responses in plants
Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... domains, including an N-terminal STAT protein interaction domain, which strengthens interactions between STAT dimers on adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain, ... STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and...
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báo cáo khoa học: " WRKY Transcription Factors Involved in Activation of SA Biosynthesis Genes" doc
... mutations as indicated in panel B Plus signs above the lanes indicate binding mixtures containing 0.5 μg recombinant GST/WRKY28 Minus signs above the lanes indicate binding mixtures without recombinant ... the PBS3 gene It is rapidly induced in plants recognizing pathogens carrying virulence factors, like in the case of Pseudomonas syringae containing AVR4 [27] A function in SA metabolism has been ... contains the two WRKY28 binding sites at -445 and -460 as determined by EMSA (Figure 4A) A similar result was obtained with a primer pair covering a smaller region containing the two binding sites...
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báo cáo khoa học: " DNA polymorphisms and haplotype patterns of transcription factors involved in barley endosperm development are associated with key agronomic traits" pot
... conserved cis-acting element, which contains two distinct protein binding sites: the prolamin-box (PB) and the GCN4-like motif (GLM) Four transcription factors (TFs) are the gibberellin-regulated ... (GAMYB), the barley leucine zippers and (BLZ1, BLZ2), and the barley prolamin box binding factor Page of 11 (BPBF) that were shown to be involved in the transcription of B-hordeins encoded by the ... barley breeding program It is known that transcription factors play an important role in controlling expression during seed development Genetic differences in the synthesis of storage proteins can...
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báo cáo khoa học: " Dissection of genetic and environmental factors involved in tomato organoleptic quality" doc
... expression of genes involved in sugar and acid biosynthesis should be designed In the second case, candidate genes should be identified and transferred into breeding lines The emerging information on ... intermediate sourness In general, all the ecotypes grown in Ercolano were given the lowest attribute intensity, those grown in Sorrento intermediate intensity and those in Sarno the highest intensity, for ... traits, respectively Abbreviations: His, Histidine; Lys, Lysine; Arg, Arginine; Gln, Glutamine; Asn, Asparagine; Ser, Serine; Glu, Glutamic acid; Thr, Threonine; pH, pH, SS., Soluble solid; Ash, Ash;...
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Molecular biology and pathogenesis of coronavirus virus and host factors involved in viral RNA transcription
... certain cisacting sequences in the 5’-UTR in negative-strand transcription The two ends of the genome, including some internal sequences in certain coronaviruses, contain multiple cis-acting sequences ... attachment protein, S, the receptor binding protein S protein has two functional domains, the receptor binding domain, which defines the tropism of the virus as well as a membrane fusion domain, which ... synthesis Zinc finger CCHC-type and RNA binding motif (ZCRB1 alias MADP1) has been identified as a possible host protein involved in RNA synthesis of coronaviruses The protein has found to interact...
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Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
... proteins, which are involved in Fe–S cluster biogenesis [20] A similar protein, ycbX, also exists in E coli [16], suggesting that this bacterium has the machinery to sulfurate the MPT in a recombinant ... difficulty in the successful expression of molybdoenzymes in the xanthine oxidoreductase family The most important factor involved in producing a functional MPT center in recombinant XDH, using the ... recombinant protein Raising the cellular redox potential by increasing aeration levels is expected to reduce the level of cysteine and increase the level of cystine in the cell, thus reducing the...
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Báo cáo khoa học: "Factors involved in Pinus radiata D. Don. micrografting" pot
... micrografting conditions, we studied effect of true age on grafting success (figure 7) In vitro establishment ability using micrografting depends on the tree age since outgrowth decreases during ageing ... to thank the Environmental Research Institute Neiker and specially Dr E Ritter and Dr S Espinel in Vitoria (Spain) for supplying the plant material used in this work Critical reading is gratefully ... primaires caulinaires de pins maritimes (Pinus pinaster Ait.) âgés sur jeunes semis cultivés in vitro, C.R Acad Sci (1989) 723–728 [5] Ewald D., Kretzschmar U., The influence of micrografting in vitro...
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Báo cáo y học: "A novel scheme to assess factors involved in the reproducibility of DNA-microarray data" pdf
... light of the dyes increasing the chance of oxidation and / or bleaching The main contributing factors identified in this study are in agreement with a number of studies involving cDNA derived ... accommodated in the VG interaction, but rather in the residual error The remaining 14.9 % variance in the VSG interaction still contains the Gene × Array interactions for S1 (7.8 %) and sampling differences ... variance in the VG interaction remaining virtually unchanged By combining S2 and S3, the Gene × Array interactions occurring specifically on S2 and S3 are pooled They are, thus, not accommodated in...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... Supplementing Lefty2 RNAi cultures with recombinant Lefty1 protein was able to block reduction in level of alkaline phosphatase staining induced by Lefty2 RNAi Alkalline phosphatase staining was ... undifferentiated cell state (Figure 2) One interesting factor identified from the screen is Lefty2, a secreted protein well known for its role in regulating Nodal signaling during embryo development Data that ... reporter Position of OCT4-SOX2 binding site within Nanog promoter is indicated Nanog transcription start site is defined by the position of the furthest 5' EST in public database (NCBI) (ATG-...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
... elicit effects in embryos in a dose dependent manner In this thesis, the possibility that Nodal signaling might, as in the in vivo scenario, likewise influence ES cell fate decision in a dose dependent ... manner in vitro is addressed Interestingly, it was found that Nodal signaling is able to arbitrate at least three distinct fate decisions in ES cells Perturbation of endogenous signaling in ES ... the individual contribution of SMAD2 and SMAD3 towards the various cell fate arbitrated by Nodal signaling Here, data showing that inhibition of SMAD3 activity blocks Nodal/Activin signaling induced...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... dishes in E14 proliferative medium containing DMEM/15% ES cell tested FBS (Invitrogen), 0.1 mM MEM nonessential amino acids (Invitrogen), 2mM Lglutamine (Invitrogen), 0.1 mM β-mercaptoethanol (Invitrogen), ... dishes in ES cell medium containing DMEM/20% ES FBS (Invitrogen, Carlsbad, CA), 0.1 mM MEM nonessential amino acids (Invitrogen), 2mM L-glutamine (Invitrogen), 0.1 mM β-mercaptoethanol (Invitrogen), ... dishes in E14 proliferative medium containing DMEM/20% ES FBS (Invitrogen,Carlsbad, CA), 0.1 mM MEM nonessential amino acids (Invitrogen), 2mM L-glutamine (Invitrogen), 0.1 mM β-mercaptoethanol (Invitrogen),...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
... defects and results in perinatal death Development 121, 1283-1299 Goldin, S N., and Papaioannou, V E (2003) Paracrine action of FGF4 during periimplantation development maintains trophectoderm ... (2007) Activin-Nodal signaling is involved in propagation of mouse embryonic stem cells J Cell Sci 120, 55-65 Oh, S P., and Li, E (1997) The signaling pathway mediated by the type IIB activin receptor ... profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state Stem Cells 23, 166-185 Wieduwilt Matthew J., (2003) SMAD3 in embryonic patterning, mesoderm induction...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... which the Lefty proteins can inhibit Nodal signaling using in vitro assays They showed that Lefty proteins can interact with Nodal proteins in solution and block Nodal from binding its receptors ... findings by culturing human ES cells with a combination of human recombinant ActRIB, ActRIIB and Cripto, thereby strengthening the idea that intact Nodal/Activin signaling is important in maintaining ... been shown that 50ng/ml recombinant Activin A, in combination with 50ng/ml FGF7 and 10nM nicotinamide can maintain human ES cells cultured on laminin substrate in the absence of feeder layers...
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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... Link to canonical Wnt pathway • • • • • • Secreted protein Member of TGFsuperfamily Involved in Nodal signaling Nodal antagonist • • • • • Rex2 • Zinc finger protein • • • Zfx • • • a-catenin ... protein complex Intracellular mediator of canonical Wnt signaling Non-SET domain protein Histone H3 methyltransferase Transcription factor Intracellular mediator of canonical Wnt signaling Transcription ... experiments involving elevation of Nodal signaling Gene Fgf5 Nestin Pax6 Ngn1 Ngn2 Gfap NeuroD1 Sox1 Msi1 Otx2 Nkx2.2 Claudin6 Mixl1 Gooscoid Nodal Fgf8 E-cadherin Cerberus1 Eomesodermin Cadherin11...
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báo cáo khoa học: " Roles of arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress" doc
... growing evidence that plant WRKY transcription factors are involved in plant ABA signaling and abiotic stress responses In rice and barley, ABA induces expression of a number of WRKY genes in ... proteins indeed function in a complex pattern in plant responses to ABA and abiotic stresses The complex roles of the three WRKY transcription factors in plant biotic and abiotic stress responses ... sensitivity as indicated by significantly increase in inhibition of germination in the overexpression line relative to that of wild type (Figure 1C) Increased inhibition of germination in the WRKY60overexpressing...
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Báo cáo khoa học: Cotton GhMPK2 is involved in multiple signaling pathways and mediates defense responses to pathogen infection and oxidative stress doc
... protein kinase and woundinduced protein kinase, two MAPKs in group A, were activated by inoculation with the tobacco mosaic virus (TMV) [10] Their orthologs in other plant species, including MPK3 ... accumulation in wild-type and transgenic plants in response to abiotic and abiotic stresses (A) Virus infection-induced H2O2 accumulation detected by DAB staining (B) Abiotic stress- induced H2O2 ... involved in oxidative stress responses GhMPK2 may serve as a crosstalk point between biotic and abiotic stress responses Further investigation is required for a more comprehensive understanding...
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Báo cáo khoa học: The yeast stress response Role of the Yap family of b-ZIP transcription factors The PABMB Lecture delivered on 30 June 2004 at the 29th FEBS Congress in Warsaw potx
... characterized and induced under oxidative stress imposed by H2O2, diamide and t-BOOH Since then, several Yap1 targets involved in ROS detoxification have been identified, including those involved in the thioredoxin ... profiles involved in arsenic response has revealed the array of genes whose transcription is enriched, including those involved in methionine metabolism and sulfur assimilation, protein degradation ... of stress agents such as cadmium, cerulenin and 1,10-phenanthroline among others, suggesting a role in the response to drug stress Indeed, several target genes encoding a set of proteins involved...
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Báo cáo y học: "RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast" docx
... transcription is induced in stress (see, for example, altered SUT expression in stress in [36]) As genome-wide datasets that identified CUTs and SUTs in budding yeast were not derived under stress ... whole-genome mapping of PolII occupancy during several different stress time courses Our major findings are: 1, transcriptional changes in response to stress are only partly reflected in mRNA abundance; ... termination becomes inefficient, leading to more transcription of full-length Nrd1 and restoration of high levels of the protein Interestingly, other genes involved in transcriptional control also show...
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báo cáo khoa học: " Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses" ppt
... AP2/EREBP transcription factors were classified into five subfamilies, including DREB/CBF (dehydration-responsive element-binding protein/C-repeat binding factor), ERF (ethylene-responsive transcription ... This finding indicates that MtCBF2 and MtCBF3/DREB1C may play a critical role in inducing the COLD-ACCLIMATIONSPECIFIC (CAS) gene, which in turn increases freezing tolerance In contrast, transcription ... up-regulated in signal transduction and ligand-receptor interaction pathways Probe sets involved in protein folding, sorting, and degradation include heat shock proteins It is worth noting that the...
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báo cáo khoa học: "The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress" ppt
... germinated in individual pots each containing the same amount of pre-wetted soil Plants were regularly irrigated for 20 days Before watering was terminated, pots were covered with tin foil to minimize ... 15-day-old pVvMYB60:GUS seedling (E) Magnification of leaf primordia in (D), showing staining of differentiating stomata (F) Detail of a pVvMYB60:GUS mature leaf, showing staining of fully differentiated ... under water stress in field-grown grapevines Aust J Plant Physiol 1999, 26(5):421-433 Schroeder J, Kwak J, GJ GA: Guard cell abscisic acid signalling and engineering drought hardiness in plants Nature...
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