... when the values of all the parameters ofthe set P are strictly equal to their counterparts in the set p andthe values of all the parameters ofthe set N are strictly equal to their counterparts ... physical/geometrical and numerical parameters to which the model appeals The physical/geometrical parameters ofthe SM form none other than the set p, whereas the numerical parameters ofthe SM can ... 1992), has a corollary (Wirgin, 2004): when the values of all the parameters, except PK ofthe set P are strictly equal to their counterparts in the set p andthe values of all the parameters of the...
... appropriateness of graphics and icons, clarity and quality of information, suitability of external links, and clarity and perceived motivating and discussion promoting characteristics ofthe learning ... interactivity; and, availability of Australian and international sources Annotations were provided for each link to inform the student about the topics and contents ofthe site The Activity Centre was designed ... Web learning environment These icons were replicated at the top left corner ofthe corresponding page and again in a navigational menu bar that appears at the bottom of each page allowing the student...
... immunoassays (ELISA, Dot Blot, Neurotoxicity assay) He participated in analyses and interpretation of data He drafted the manuscript AG has been involved in analyses and interpretation of data and ... mentored primary authors, helped to analyze the data and make conclusions, prepared final version of manuscript All authors read and approved the final manuscript Page 13 of 15 Declaration of competing ... laboratories Another important aspect ofa dual vaccine is related to the safety issues Since the majority of people including children and elderly are vaccinated with influenza vaccine yearly and...
... Ivan Sutherland’s PhD thesis in 1963, parametric change andthe representation which could adapt to the change is one ofthe core functions (Sutherland, 1980) Nowadays, a parametric model can ... opening (b) Backward ray tracing of opening (c) Forward ray tracing of table (d) Backward ray tracing of table 118 xi List of Tables Table 4.1: Data types required for the ports ofthe RayTracer ... reasonably fast and thousands of generations are exploded and evaluated by evolution algorithm The details ofthe process including the modeling details, the evaluation method and evolution algorithm...
... observation, as well by the peasant as by the man of erudition The fact, that man, "made of one blood, can dwell" in all the varieties of climate, "on the face ofthe whole earth," and can sustain himself, ... gratification of appetites, regardless of others, is the strongest feature of barbarism We see then, even as a dictate of refinement, that the use of tobacco should be abandoned; and it has been abandoned ... charge ofthe patient She was much emaciated The discharge from the bowels continued unabated, and was often attended with severe pain and great prostration of strength The salivation was accompanied...
... hirsutellin AA Viegas et al involvement ofa catalytic pair constituted by an acid anda base on each side ofthe hydrolyzed bond [16] E96 and H137 in a- sarcin and E58 and H92 in RNase T1 act as the base ... Pozo A & Gavilanes JG (2001) RNase U2 and alphasarcin: a study of relationships Meth Enzymol 341, 335–351 ´ Lacadena J, Alvarez-Garcı´ a E, Carreras-Sangra N, ´ Herrero-Galan E, Alegre-Cebollada ... accessible surface area ofthe corresponding side chains very low Desolvation ofthe charged groups should affect their pKa values, increasing the pKa of carboxylates and decreasing the pKa of...
... more antibacterial than dermaseptin (34 amino acids) [19], peptides consisting of amino acids 7–22 of parabutoporin (an a- helical part having the four amino acids LAKK identical to mastoparan) and ... explain the higher antibacterial activity on Gram-negative bacteria for parabutoporin compared to opistoporin Also with magainin analogs, an increase in antibacterial activity against Gram-negative ... synthesized chemically and preliminary studies on antibacterial activity showed that the quality and biological activity of native and synthesized toxins were identical Due to a shortage of native...
... Singapore The Straits of Malacca The Straits of Singapore The Straits of Singapore The Straits of Malacca The South China Sea The South China Sea The Straits of Singapore The Straits of Malacca The ... Sarawak in the north of Kalimantan Kuala Lumpur, the national capital, Labuan UNEP/SCS – National Report Malaysia and Putra Jaya form the Federal territories The multiracial population of Malaysia ... meters and with a tidal stream of over knots The West Coast of Peninsular Malaysia has an equatorial climate, with an average annual rainfall of more than 2500mm anda daily temperature that ranges...
... cycles of 94 °C for 20 s, 52 °C for 20 s and 70 °C for min; then a final extension at 72 °C for 10 The primers for lac1 PLAC1F (5¢-AGCTTT CATTCCCAGTGATTG-3¢) and PLAC1R (5¢-AACGAG CTCAAGTACAAATGACT-3¢) ... and sequenced as above The fulllength cDNA of lac1 was then generated by 3¢-RACE using the primer 5¢-TCTCAACCGTCGACAGCAG TGTTCGTG-3¢ designed from thesequenceofthe extreme 5¢ end of lac1 The ... developmental cycle: early, middle and late substrate colonization stages (4, and 12 days); pinhead stage (day 14), button stage (day 18), egg stage (day 21), elongation stage (day 22) and mature stage...
... (Forward) TAAGCTACTACGTAAAGAATATATC (Reverse) GATAAGGTACCTGCACTGACACGGATGAAAGC (Forward) CATATATTCTTTACGTAGTAGCTTA (Reverse) FEBS Journal 272 (2005) 4091–4102 ª 2005 FEBS 4099 Molecular characterization ... PP2500 and ANKHD1 variant In this study we focus on the biochemical and functional characterization ofthe novel VBARP-L and VBARP-S transcripts Bioinformatics analyses show that VBARP-L and VBARP-S ... AAAGC-3¢ and reverse 5¢-CTAGACTCGAGCCTAAT TTATATTTGCTCCTTGTGC-3¢ b-Actin primers were designed as follows: forward 5¢-CTACAATGAGCTGCG TGT-3¢ and reverse 5¢-AAGGAAGGCTGGAAGAGT-3¢ Cell survival and apoptosis...
... in thesequenceofthe C-terminal heptapeptide of NKA, another peptide ofthe tachykinin family that binds the NK-1 and NK-2 receptors [HGly8] NKA(4–10) is as potent as NKA and [Ala8]NKA on rabbit ... between [Ala9]SP and [Gly9]SP on the one hand, and [b2-HAla9]SP and [HGly9]SP on the other hand Indeed, the higher pharmacological potency of [b2-HAla9]SP compared to [HGly9]SP suggests that the methyl ... similar results All assays were done in parallel experiments with control at t ¼ for each peptide The percentage of degradation was calculated by comparing the area ofthe peaks ofthe intact...
... induced against the linear isoform ofthe HNE peptide Although the binding pattern may be somewhat blurred by these antibodies and by the polyclonal nature ofthe sera, the importance of residues ... bonds stabilizes a conformational epitope ofthe apical membrane antigen-1 of Plasmodium falciparum [27] Although the sequential epitope of VP1 of foot and mouth disease does not contain intramolecular ... microtiter plates increased at high pH (Fig 3A) Under the basic conditions optimal for coating, the HNE peptide was at least partially oxidized andthe signal ofthe reduced species increased as a result...
... attracting them, like I said that’s for another book There are certain do’s and don’ts that can make or break a relationship that many people just don’t realise The repercussions of actions and ... head all ofthe time is come clean ASAP You’re going to have to come clean as soon as you can and accept that whatever happens is always going to be your fault Your partner may leave you, and ... and starting again Arguments happen and you need to deal with that What makes the relationship work is how these arguments actually affect you both If you can accept them as arguments and get...
... PIVceeding ofthe ACM SIGMOD Conference on Management of Data, May 1994 [CS92] RakeshChandmand Arie Segev.ManagingTemporalFinancial Data in anExtensible Database.In Proceedings ofthe International Conference ... component) supports relational data as well as sequencedata, using a novel design paradigm of enhancedabstractdata types (BADTs ) The systemimplementation basedon this paradigmallows sequence relational ... up the dimensions) There are also severalopen researchissuesin the design of systemsbasedon the E-ADT paradigm, and in extensions ofthe paradigm to handle optimizations that spandata types Acknowledgements...
... indicated that the b-aryl ether cleavage enzyme accumulated and was stable in the extracellular fraction The extracellular fraction of 2BW-1 generated abundant GG and 4MU from GOU by cleaving the ... radiolabeled water was not observed with guaiacol It was clear that the b-aryl ether cleavage enzyme catalyzed the addition of two molecules of H2O (at Ca and Cb positions) and cleavage the b-aryl ... 12 Masai, E., Katayama, Y., Nishikawa, S., Yamasaki, M., Morohoshi, N & Haraguchi, T (1989) Detection and localization ofa new enzyme catalyzing the beta-aryl ether cleavage in the soil bacterium...
... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R, 5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GTcDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT CCAAGTGC-3¢ for GT-cDNA2 (3043 bp) RNA isolation and ... 5¢-RACE andthe two alternate 3¢-ends of exon 12 were deduced by 3¢-RACE, and are delineated by the full-length cDNA clones, GT-cDNA1 and GT-cDNA The two putative polyadenylation sites (ATTAAA) are ... genomic sequence revealed two putative polyadenylation (ATTAAA) signals: one is located 18 bp upstream from the poly (A) of GT-cDNA1 and another is located 11 bp upstream from the poly (A) of GT-cDNA2...
... enzymes, such as xylanase and mannanase P hilaris cellulase was proposed to belong to GHF andthe signature sequenceof GHF was found in the aminoacid sequence (Fig 3) GHF is the largest GHF and includes ... the deduced amino-acid sequenceThe ORF consisted ofa protein of 325 amino acids The molecular mass of P hilaris Fig Comparison of amino-acid sequences for potential catalytic proton donor and ... was conserved in the deduced amino-acid sequenceof P hilaris cellulase (Fig 3) The conserved potential proton donor and Analysis of N-terminal amino-acid sequence, cDNA sequenceand deduced amino-acid...
... at the beginning ofthe chromatogram (peaks 1*, 2*, 3*, and in Fig 5A) For peaks and 2, molecular masses of 2025 and 2041 Da were obtained as before (Table 1) The molecular mass of peak 3* was ... additional 162 Da) in the material of peak (Fig 3A, B) and both galactose and fucose (the additional 146 Da) residues in peak (the data for the Ru strain are shown in Fig 4) These results correlated closely ... for films ofthe intact and trypsin-treated Ru PVX andthe intact ST mutant preparations To compare the waterabsorbing capacity ofthe different PVX variants, we measured their FTIR spectra in dry...
... CCT were quantitated by the use ofa phosphorimager and expressed as a fraction ofthe maximum amount of bound labeled tubulin and plotted as a function ofthe ratio of labeled : unlabeled tubulin ... immediately after dilution or after h of incubation at 30 °C b-T1 behaviour was similar to that of b5 tubulin [33] At early times, the bulk ofthe radioactivity migrates as a broad band with a slower ... nondenaturing PAGE; the ratios of labeled to unlabeled tubulins are indicated on the top of each lane andthe arrow indicates the position ofthe b-tubulin/CCT complex (B) The amounts ofthe tubulin...