stress cell damage and its possible prevention by alpha tocopherol cardiovascular and other cells
Báo cáo y học: "Mast cell tumours and other skin neoplasia in Danish dogs - data from the Danish Veterinary Cancer Registry" potx
... Acta Veterinaria Scandinavica 2010, 52:6 http://www.actavetscand.com/content/52/1/6 Page of Fine needle aspirations of MCT are usually rich in cells as these exfoliate easily and cytology is usually ... Veterinaria Scandinavica 2010, 52:6 http://www.actavetscand.com/content/52/1/6 Page of in the skin, subcutis or adnexa and had a diagnosis established by cytology or histopathology Another 21 cases ... al.: Mast cell tumours and other skin neoplasia in Danish dogs - data from the Danish Veterinary Cancer Registry Acta Veterinaria Scandinavica 2010 52:6 Publish with Bio Med Central and every...
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Clinical significance of cancer stem cell markers and other related proteins in colorectal carcinomas biological and methodological considerations
... initiated by a single mutation that disrupts the regulated asymmetric division in stem cells The mutated “cancer” stem cells then differentiate into other committed “cancer” daughter cells This ... differentiate into committed daughter cells (C) The daughter cells eventually form a fully transformed cell by accruing other mutations (D) 15 1.5 The Prognostic and Predictive Markers Examined in ... normal epithelial cell including stem cells and their niche (A) A tumour is initiated upon a mutation (B) These mutated cancer stem cells disrupt normal asymmetric division and differentiate...
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Báo cáo y học: " Cellular and molecular mechanisms of cigarette smoke-induced lung damage and prevention by vitamin c" ppsx
... protein damage is followed by apoptosis, which is accompanied by lung damage Apoptosis is a tightly regulated mechanism of cell death, a process by which the cells eliminate unwanted damaged cells ... apoptosis in A549 cells Figs 7A and 7B show that compared to PBStreated cells, the per cent of cells with DNA damage is significantly high (p < 0.001, n = 3) following treatment of A549 cells with 50 ... B P-BSQ+vit C Figure and lung damage caused by intratracheal instillation of p-BSQ in guinea pigs and prevention by vitamin C Apoptosis Apoptosis and lung damage caused by intratracheal instillation...
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Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx
... invasive cells was determined by counting stained cells and then normalizing to the number in the case of control shRNA-transfected cells All numerical data represent the means and standard deviations ... the means and standard deviations of three independent experiments Cont, pCMV ⁄ HA-transfected cells; WT, OccWT-overexpressing cells; DE9, OccDE9-overexpressing cells; +, 2-APB-treated cells; *P ... Cont, pCMV ⁄ HA-transfected cells; WT, OccWT-overexpressing cells; DE9, OccDE9-overexpressing cells; *P < 0.05 the OccWT-overexpressing and OccDE9-overexpressing cells (Fig 7C) In addition, 2-APB...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... HEK293 T cells expressing Vpr and VBARP by cotransfecting Vpr and VBARP-His using calcium phosphate transfection Forty-eight hours post transfection, the cells were lysed and the whole cell proteins ... tested cell lines and this siRNA was used in subsequent assays Following transfection of VBARP siRNA into HeLa and NT2 cells, physical observation revealed that cell death occurred in a dose- and cell- dependent ... affect cell viability However, at a concentration of 100 nm the percentage of cell viability in VBARP and control siRNA-treated cells was 20 and 75, respectively The number of viable cells in...
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Primary Prevention by Nutrition Intervention in Infancy and Childhood ppt
... Corthesy/Switzerland Dr Bianca-Maria Exl-Preysch/ Switzerland Dr Marie-Claire Fichot/Switzerland Prof Ferdinand Haschke/Switzerland XIV Mr Charly Huber-Haag/Switzerland Dr Martin Kussman/Switzerland Mr ... thermogenesis, for example, the energy used to digest food and process and store nutrients, and to thermogenesis induced by drugs or other substances and by exposure to cold (adaptive thermogenesis) Energydissipating ... Primary Prevention by Nutrition Intervention in Infancy and Childhood Nestlé Nutrition Workshop Series Pediatric Program, Vol 57 Primary Prevention by Nutrition Intervention in Infancy and Childhood...
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Báo cáo khoa học: Inhibition of urokinase receptor gene expression and cell invasion by anti-uPAR DNAzymes in osteosarcoma cells pot
... controls (P < 0.01) (Fig 6) Saos-2 cells treated with the active Dz720 had 42% and 17% fewer cells on the lower side of the filter compared with untreated control cells and cells treated with Dz720 mutant, ... blue and visualized by microscopy (A) Untreated cells (B) Cells transfected with Dz720 mutant (C) Cells transfected with Dz720 active (D) Graphical representation of toluidine blue-stained cells ... control and mutant DNAzyme-treated cells (Fig 5) Inhibition of Saos-2 cell invasion by Dz720 The plasminogen activator system can promote the breakdown of ECM and facilitate cell invasion and metastasis...
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Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy pdf
... peptide and secondary structure determination Figure summarizes sequential and medium range NOEs as well as slow-exchanging amide protons for K4L7W and its diastereomer in DPCho and SDS micelles Micellar ... mammalian cells by diastereomers of melittin: structure–function study Biochemistry 36, 1826–1835 15 Oren, Z & Shai, Y (2000) Cyclization of a cytolytic amphipathic alpha- helical peptide and its diastereomer: ... observed for K4L7W in the DPCho micelles (Fig 4A), and other helical-type structures, albeit less defined, are observed for K4L3l4W in the DPCho micelles (Fig 4B) and K4L7W in the SDS (Fig 4C) A...
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Targeting New Pathways and Cell Death in Breast Cancer Edited by Rebecca L. Aft docx
... vitro by incubation of the fusion proteins with the target cancer cells, coculture of the immunoapoptotin-secreting lymphoma Jurkat cells with target cells, or direct transfection of target cells ... compared to the parental MCF-7 cells, which not express Fas and treatment of the MCF7/LTED cells with estradiol resulted in a marked increase in Fas ligand (FasL) in these cells (Song et al 2001) It ... in neoplastic cells would be appropriate to targeting breast cancer cells (Couto and High, 2010; Wen et al, 2008) Again, the antibodies or ligands that recognize breast cancer cells are utilized...
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Báo cáo sinh học: "The impact of Metastasis Suppressor-1, MTSS1, on oesophageal squamous cell carcinoma and its clinical significance" doc
... (Amersham, UK), and photographed using a UVITech imager (UVITech, Inc.) In vitro cell growth assay Cells were plated into 96-well plated at 2,000 cells/ well after a period of incubation Cells were ... grade Material and methods Cell lines and human oesophageal specimens This study used three human oesophageal squamous cell carcinoma cell lines and an oesophageal adenocarcinoma cell line Moderate-differentiated ... rehydration, 30,000 cells were added to each well After 40 of incubation non-adherent cells were washed off using BSS buffer The remaining cells were fixed with 4% formalin and stained with 0.5%...
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báo cáo hóa học:" Unsaturated phosphatidylcholines lining on the surface of cartilage and its possible physiological roles" potx
... compare the three pairs, PLPC and DPPC, POPC and DPPC, and PLPC and POPC, separately There were significant differences between POPC and DPPC (p = 0.002), and PLPC and POPC (p = 0.003) There was ... pooled together and soaked in Folch reagent The chloroform phase containing both PC and non-PC species were obtained The PC and non-PC species were then separated from each other by using a 100 ... is produced by alveolar Type II cells in the form of lamellar bodies, which are secreted onto the alveolar surface [3] Studies indicate that SAPL is also synthesized and secreted in other parts...
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o cáo hóa học:" The impact of Metastasis Suppressor-1, MTSS1, on oesophageal squamous cell carcinoma and its clinical significance" docx
... (Amersham, UK), and photographed using a UVITech imager (UVITech, Inc.) In vitro cell growth assay Cells were plated into 96-well plated at 2,000 cells/ well after a period of incubation Cells were ... grade Material and methods Cell lines and human oesophageal specimens This study used three human oesophageal squamous cell carcinoma cell lines and an oesophageal adenocarcinoma cell line Moderate-differentiated ... rehydration, 30,000 cells were added to each well After 40 of incubation non-adherent cells were washed off using BSS buffer The remaining cells were fixed with 4% formalin and stained with 0.5%...
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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... Extra -and intracellular Muc16 expression by MOVCAR cells Extra -and intracellular Muc16 expression by MOVCAR cells (A) MOVCAR-10 cells were labeled with 618F (grey line) and analyzed for cell surface ... natural killer cells, the cell surface bound MUC16 is important for binding of the ovarian tumor cells to the mesothelial cells that line the peritoneal cavity Since the MOVCAR cells shed Muc16, ... blotIdentification of soluble Muc16 and MUC16 by Western blotting Purified MUC16 (25 μg total protein/lane) from MOVCAR-2 (lane 1) and OVCAR-3 cells (lane 2) was electrophoresed by SDS-PAGE and probed with a panel...
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Báo cáo hóa học: " Cell Creeping and Controlled Migration by Magnetic Carbon Nanotubes" ppt
... application of the magnet a Cells treated with the CNTs and exposed to the magnetic field b control cells not exposed to the magnetic field but treated with the CNTs and c control cells without CNTs but ... had an inter-circle line distance of 500 lm The cells were seeded at a concentration of 105 cells/ well and incubated for h to allow cell attachment Cell culture medium was then replaced with CNT-modified ... cells were identified in the well and their exact position determined at 0, 24, 48 and 72 h after placement of the magnet Figure compares the displacements of the cells treated with the CNTs and...
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Cell Metabolism Cell Homeostasis and Stress Response Part 15 doc
... normal and tumor cells in culture Prog Biochem Pharmacol 10: 135–66 Hu, C.-D., Chinenov Y & Kerppola T.K 2002 Visualization of interactions among bZIP and Rel family proteins in living cells using ... therapy: oncogene and nononcogene addiction Cell 136: 823-837 Manders, E.M.M., Kimura H & Cook P.R 1999 Direct imaging of DNA in living cells reveals the dynamics of chromosome formation J Cell Biol ... replication in primary mammalian cells Genes Dev 14:2855-2868 Kimura, H., Sugaya K & Cook P.R 2002 The transcription cycle of RNA polymerase II in living cells J Cell Biol 159: 777-782 Kolthammer,...
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Cell Metabolism Cell Homeostasis and Stress Response Part 1 pdf
... out by oligosaccharides, and particularly, by oligoglucans 2 Cell Metabolism – Cell Homeostasis and Stress Response Oxidative stress and reactive oxygen species Oxidative stress is defined as the ... high temperatures and mechanical damage (Tsugane et al., 1999) The presence of heavy metals in the field, like pollution by lead (Pb) induces oxidative stress that damages cells and their components ... (•O2H) and hydrogen peroxide (H2O2), are generated in all aerobic cells as byproducts of normal metabolic processes In general, under various conditions of environmental stress, plant cells show...
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Cell Metabolism Cell Homeostasis and Stress Response Part 2 doc
... the generation and removal of ROS within cells In plant cells, ROS are generated in large quantities by both constitutive and inducible pathways, but in normal situations, the cellular redox ... aerenchyma in stems and roots of aquatic species tolerant to flooding, which usually occurs by cell separation during development (esquizogeny) or by lysis of cortical cells and cell death (lysogeny) ... accumulate in flooded soils can be produced 18 Cell Metabolism – Cell Homeostasis and Stress Response both by plant roots (ethanol and acetaldehyde) and the metabolism of anaerobic microorganisms...
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Cell Metabolism Cell Homeostasis and Stress Response Part 4 ppt
... temperatures Plant Cell Environmental, Vol.23, pp.893-902 38 Cell Metabolism – Cell Homeostasis and Stress Response Zhu, J-K (2001a) Cell signaling under salt, water and cold stresses Current ... peroxide (H2O2), and hydroxyl radical (OH•) ROS also 40 Cell Metabolism – Cell Homeostasis and Stress Response include the singlet oxygen (1O2), which is not generated by O2 reduction but by energy ... ОН• (2) 42 Cell Metabolism – Cell Homeostasis and Stress Response This reaction can also be catalyzed by univalent cuprous ion, which is oxidized to divalent ion Both the oxidized iron and cuprum...
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Cell Metabolism Cell Homeostasis and Stress Response Part 6 docx
... upon a severe cell cue the elicited stress responses may be insufficient to guarantee cell survival and cell death may occur The simplicity of yeast and its amenability to manipulation and genetic ... and the prevention of food and beverage spoilage by resistant species In most strains of S cerevisiae, acetic acid is not metabolized by glucose-repressed yeast cells and enters the cell in the ... stress conditions, such as those imposed by low water activity and by the presence of cytotoxic compounds Yeast cells react to adverse conditions by triggering a stress response, enabling them to adapt...
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Cell Metabolism Cell Homeostasis and Stress Response Part 8 pptx
... or to band inhibits activity (Real-Hohn et al., 2010) Actin is involved in a variety of cell functions that include contractility, cytokinesis, maintenance of cell shape, cell locomotion and organelle ... yeast: the role of intracellular polyols J Gen Microbiol 1972; 72:589-591 110 Cell Metabolism Cell Homeostasis and Stress Response Campanella ME, Chu H & Low PS Assembly and regulation of a glycolytic ... programmed cell death in the pathogenic fungus Candida albicans Proc Natl Acad Sci USA, Vol 103, pp 726731 Phillips, A.J., Sudbery, I & Ramsdale, M (2003) Apoptosis induced by environmental stresses and...
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