... scared? AND Ma'am COUN Well Ma'am what is the matter? AND It is that COUN What? AND I have no wax candles, but only dips COUN The simpleton! And where are the wax candles I bought a few days ... rascal of a page AND I say, Criquet! COUN Cease that "Criquet" of yours, stupid, and call out "Page." AND Page then, and not Criquet, come and speak to missis I think he must be deaf Criq Page! ... ANDRÉE) Get along with you, you hussy I drink with a salver I tell you that you must go and fetch me a salver AND Criquet, what's a salver? CRI A salver? AND Yes CRI I don't know COUN (to ANDRÉE)...
... of England and VIII of Scotland had moped away his bitter old age; here, years and years ago, Charles Edward's mother, the beautiful and brilliant grand-daughter of John Sobieski, had pined away, ... morose and besotten old Stuart Be this as it may, Charles Edward, however degraded, was able to command himself when he chose, and, for one reason or another, he did choose to command himself and ... Charles Edward's nature when he was young and, for all his reverses, still hopeful; as he grew older, as deferred and disappointed hopes, and endured ignominy, made him a middle-aged man before...
... San Diego, CA, USA) This first protocol included a pseudo-Brownian rigid-body docking, an extended force field, and a soft interaction energy function precalculated on a grid To validate the docking ... by APC were obtained from time courses of inactivation for FVaLeiden as described in Materials and methods ND, Not determined; k506 and k306 could not be determined in FVaLeiden because of the ... below) and a model structure for the three A domains of FVa (with some modifications at the Arg506 and Arg306 sites, see below) were used in two different automated docking procedures Rigid-body-docking...
... ostream::osfx to flush stdout and stderr after each insertion More on Loops: Suppose you already have a program and you want to add a loop around it to make it run many times Let us modify the program ... and setprecision are added This seems to have fixed the problem Here the ios::fixed causes the real number to be output in fixed point format instead of exponential format Program 6-2 #include ... for three scores, found the average and letter grade and displayed these values We could choose the first score as the loop control variable If a negative number was entered for the first score,...
... LPS/IFN-induced NO production We compared the effects of a-T and its derivatives TA and TN with that of TS on LPS/IFN-induced NO production The additions of a-T, TA and TN did not induce NO production ... that TA and TN also decreased the enhancing effect of TS on LPS/IFNinduced NO production Fig Effects of a-T and its derivatives (TA and TN) on LPS/IFNinduced NO production in VSMC treated without ... pathways and TS effects In this study, we found for the first time that TS enhanced LPS/IFN-induced NO production in VSMC, and that the TS effect would be induced through upregulation of PKCa In addition,...
... the data, the second one to find the difference of each score from the mean and the third one to find store the square of deviation of each score The mean is calculated by adding all valid scores ... /************************************************* Read scores from a file into an array Keep track of number of scores entered Find difference of each score from the Mean Square the differences and add the squares find stadard deviation ... "CA","C2","C3","C4","C5","C6","C7","C8","C9","C10","CJ","CQ","CK", "DA", "D2 ", "D3 ", "D4 ", "D5 ", "D6 ", "D7 ", "D8 ", "D9 ", "D1 0","DJ","DQ","DK", "HA","H2","H3","H4","H5","H6","H7","H8","H9","H10","HJ","HQ","HK",...
... were done as described by [8] Determination and analyses Extraction and determination of ginsenoside production were determined as reported previously [9] Experimental design and data analysis ... concentrations investigated, 30 g/L sucrose enhanced biomass yield (180 g/L FW, and 10.8 g/L DW), and ginsenoside production (total ginsenoside production upto 6.56 mg/g DW) Further increase of ... ginsenoside yield of ginseng cell culture Materials and methods Stock cell culture and culture condition Stock suspension cells of P ginseng were maintained in MS medium The cultural conditions were done...
... mg of peroxidase was added and the reaction mixture was stirred for additional 12 h The resultant precipitate was collected and washed three times with 10 mL water and centrifuged at 4000 g for ... dimethylsulfoxide -d6 ) (Fig 2) For the Ca position reduction of GOU aO in DHP-GOU (Ca carbonyl type), g each of DHP-GOU and sodium borohydride were dissolved in dioxane/methanol (4 : 1) and stirred for ... not detected until cultures were 6-days-old In 7-day-old cultures, we detected weak activity The activity increased for more days and then decreased (data not shown) This result suggested that...
... were then pooled and reacted with radiolabeled DFP SDS/PAGE followed by fluorography yielded bands only in the 35–40 kDa range (data not shown) The same multiple bands were detected with the anti-(178/191 ... products obtained from bovine genomic DNA, digested and modified as described above, were analyzed on agarose gel Two distinct, prominent bands were obtained in the case of genomic DNA digested with HincII ... tryptase cDNAs Nucleotide numbering begins at the first nucleotide of the preprosequence Stop codon (r) and polyadenylation signal (underlined) are indicated (A) BLT cDNA and deduced amino acid sequence...
... that SerRSDC13 was stable and correctly folded in pull-down experiments, we determined kinetic parameters for full-length Saccharomyces cerevisiae (Sc)SerRS and truncated enzyme in standard aminoacylation ... prior to addition of 32P-labeled tRNAs Reaction mixtures were subjected to electrophoresis on a nondenaturing polyacrylamide gel Free and proteinbound tRNA was visualized by radiography, and the ... of Pex21p and SerRS were preincubated for 10 prior to addition of 3¢-radiolabeled tRNASer in binding buffer containing 150 mM sodium chloride and 2.5 mM MgCl2 The complexes were analyzed by 5%...
... antibodies directed against SACCP on the same nitrocellulose membrane showed that the peroxidase-activity-stained band and the SACCP immunogenic band colocalized (Fig 8) Using MS, The two additional ... in Tris ⁄ EDTA buffer (0.1 mm Tris ⁄ HCl, 0.1 mm EDTA, pH 8.0) and submerged directly into liquid nitrogen and stored at )80 °C until used RNA isolation and DNase treatment were carried out using ... separated by SDS ⁄ PAGE and stained with CBB R-250 The polypeptide migrating with a corresponding molecular mass to the anti-MCA2590 immunogenic band is indicated A B Fig Dot-blot and biotinylation...
... In the present study, we mutated aromatic and charged residues, suppressed the C-terminal cysteine or introduced cysteines at other positions, and deleted more or less extended C-terminal segments ... extracts and in the medium; the standard errors were obtained from five independent experiments For other individual mutations (K11A, E13A, R16A, K25A and D2 9A), the cellular activities ranged from ... (top) and secreted (bottom) molecular forms obtained with and without QN, in the presence of Triton X-100 and sodium deoxycholate Note that mutants I3C/C37S, I3C/KDEL and I3C/stop37 produced homomeric...
... and structural studies and for the study of MG Results Rationale for the construction and testing of AChR ECD variants N-Terminal addition of the FLAG peptide (DYKDDDDK) or addition of the first ... structures and that the secondary structures of a non-a ECD (b) and the a ECD resemble one another, being largely composed of b-structure Thirdly, conformation-dependent anti-b and anti-c (mAbs 73 and ... results showed that the addition of a Pro residue and the presence of common epitopes ⁄ tags used for purification influenced the expression yield When the widely used 6-HIS tail was added to the C-terminus...
... and four glutamic acids) and four basic residues (one lysine, two arginines and one histidine), which might form internal salt bridges between residues D4 or E5 and R8, between E7 and K11, and ... coupling of the synthetic peptides to exposed lysine residues occurred randomly and did not affect enzymic activity We deduced the mean number of peptides added per tetramer from the apparent ... wildtype), and the CC-QN construct The distribution of unlinked subunits and disulfide-linked dimers is consistent with the distribution of active monomers and dimers, as indicated by nondenaturing...
... studies, the NF-jB and c-Jun binding sites were identified in the Fig LPS-induced and CpG-ODN 1826-induced CMV IE enhancer/ promoter activity in RAW 264.7 cells is inhibited by IjBaSR and the dominant ... factors and MyD88 Our results indicate that LPS-induced and CpGODN-induced signals use TLR/IL-1R signaling molecules for CMV IE enhancer/promoter activation Materials and methods Cell culture and reagents ... experiments) or CpG-ODNs (3 lM or as indicated in the individual experiments) treatment for h or as indicated in the individual experiments The cells were harvested, washed and lysed by freeze-thawing...
... washed and reincubated with fresh medium for up to 120 at 37 °C The overlying medium and cells were separated at the reincubation times indicated and examined for radioactivity (see Materials and ... then washed and reincubated with fresh medium for up to 120 at 37 °C The overlying medium (efflux medium) and cells were separated at the reincubation times indicated and examined for radioactivity ... performed and are expressed as the mean ± SD of three determinations In contrast with by cells 125 I-Tf, 125 I-sMTf is markedly degraded Considering the results described above showing that sMTf may...
... Hilbert, o and Courant was carried forward in the U.S by Fritz John, James Stoker, and Kurt Friedrichs, and they and their students developed many important ideas that reached beyond rigorous differential ... instabilities described by Lorenz and Keller [104] f Irregular ecological dynamics studied by Ricker andMay [48] g Random processes, including the Law of Large Numbers and ergodic and other limit ... period of A We say that Φ(t) is a fundamental matrix for this system if d = A(t)Φ dt and Φ(0) is nonsingular Note that if d( t) denotes the determinant of Φ(t), then dd = tr[A(t) ]d and d( 0) = dt...
... structure and on its compliance with laws and regulations as they relate to the Fund We conducted our audits in accordance with generally accepted government auditing standards Those standards require ... Federal Deposit Insurance Corporation (FDIC) Our audit disclosed that the Fund’ statements of financial position as of s December 31, 1991 and 1990, and its related statements of income and fund ... the difference between amounts advanced and the expected repayment, net of all estimated liquidation costs As of December 1,199l and 1990, the allowance for losses equaled $22.4 billion and $16.6...