schmidt s synthesis of lta type i from s aureus
Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf
... was excised from expression plasmid pBSIIKS-hIdu obtained from E F Neufeld, UCLA School of Medicine, Los Angeles, CA, USA The 2.2-Kb human IDUA cDNA was excised from this plasmid by digestion ... provides high levels of IDUA expression in recipient cells in vitro and may also provide sufficient expression in deficient cells and tissues in vivo to similarly reduce or prevent storage accumulation ... conserves only three of the nine HIV-1 genes and relies on four separate transcriptional units for the production of transducing particles This system offers significant advantages for its biosafety...
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routledge history of philosophy volume i from the beginning to plato
... Battle of Leuctra 370–69 Liberation of Messenia 367 Death of Dionysius I of Syracuse: succession of Dionysius II 360 Accession of Philip II of Macedon: beginning of rise of Macedon to hegemony in ... local competitors) as the individual himself In its festival life a city displayed itself and its divisions and citizens observed their own social as well as political place in it It is not by chance ... others, as he spins false tales of his identity to all he meets, not only raises moral issues, it also raises issues about language and representation Odysseus’ briefest deceptive tale is also...
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Báo cáo vật lý: "Synthesis of Hydroxyl Radical Scavengers from Benzalacetone and its Derivatives" pot
... synthesis from benzaldehyde and its derivative Indonesian Journal of Chemistry, 5(2), 22–27 Arty, I .S & Handayani, S (2007) Synthesis and activity test of benzalacetone and its derivatives compounds ... (ppm) Figure 3: Antioxidant activity of dibenzalacetone and its derivatives From the graphs the IC50 values of the samples by using regression linear equation are shown in Table The result of the ... Halliwell, B & Guttridge, J.M.C (1989) Free radicals in biology and medicine Tokyo: Japan Scientific Societies Press Walia, H., Kumar, S & Arora, S (2007) Analysis of antioxidant activity of methanol...
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Báo cáo y học: " Species-independent bioassay for sensitive quantification of antiviral type I interferons" pdf
... advantages of the classical antiviral assay with the convenience of luciferase reporter assays Results and discussion Establishment of a luciferase-based antiviral IFN assay Using our reverse genetics ... (Fig 4B) This strongly indicates that it is type I IFNs which are causing the antiviral effect against RVFV-Ren Similar to the conventional virus inhibition or GFPvirus-based IFN bioassays, it ... might as well be adapted for IFN-sensitive nonBSL3 viruses with a broad host range such as Vesicular stomatitis virus or Newcastle disease virus Figure RFVFV-Ren antiviral assay on cells lacking...
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Báo cáo khoa học: Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli pptx
... denaturation–renaturation step was limited to 20% In addition to E coli expression, the expression of a GluV8 family protease from Bacillus licheniformis was achieved using Bacillus subtilis as a host [9], and from Streptomyces ... N-terminus of the six serine proteases Spl from S aureus is Glu [28] Although a glutamyl endopeptidase from Bacillus licheniformis possesses the sequence Lys94-Ser-Val-Ile-Gly98 around the processing ... be investigated by in vitro mutagenesis As an initial approach, two key amino acids were chosen: Ser237 and Val69 GluV8 is a serine protease, the active site of which consists of the His119, Asp161...
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Báo cáo khoa học: Promoters of type I interferon genes from Atlantic salmon contain two main regulatory regions docx
... protein [54] The chicken birnavirus, infectious bursal disease virus, has also been shown to inhibit transcription of IFN genes [55], which suggests a common immunosuppressive mechanism of this ... PR -I is most activated upon poly (I: C) induction, at least in nonimmune cells, producing high amounts of short IFN transcripts, while the distal PR-II is poorly induced in TO cells Discussion In ... two insertions ⁄ deletions (Fig 1) This was surprising because SasaIFN-a1 was originally identified as a short transcript (829 nucleotides) and SasaIFN-a2 as a long transcript (1290 nucleotides)...
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báo cáo hóa học: " Clinical usefulness of the screen for cognitive impairment in psychiatry (SCIP-S) scale in patients with type I bipolar disorder" doc
... patients suffering from schizophrenia [24] The present investigation was designed to assess the sensitivity, reliability and validity of the SCIP for detection and quantification of cognitive impairment ... manic episodes, Feasibility The relatively brief training session for the SCIP administration resulted in a mean kappa index of agreement in The SPSS version 12.0 statistical software was used ... analysis of variance of baseline subtest and total scores, respectively Testretest reliability was assessed with intra-class correlation coefficients (ICC) between the first and second administrations...
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Báo cáo khoa hoc:" Absence of autoantibodies connected to autoimmune polyendocrine syndrome type I and II and Addison''''s disease in girls and women with Turner syndrome" docx
... 21-Hydroxylase (21-OH) Steroid hormone synthesis Description of important autoantigens in Addison 's disease, Addison 's disease with premature ovarian failure (POF), APS I and APS II Patients with Addison 's ... Steroid hormone synthesis Monoaminergic and serotonergic biosynthetic pathways Rate-limiting enzyme in catecholamine biosynthesis Rate limiting enzyme in the synthesis of serotonin APS II 21-Hydroxylase ... disease, insulin dependent diabetes mellitus (IDDM), Addison 's disease, rheumatoid arthritis, myasthenia gravis, vitiligo, alopecia, pernicious anaemia and hypoparathyroidism, but the association...
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Nghiên cứu chế tạo canxi hydroxyapatit từ vỏ sò thừa thiên huế synthesis of hydroxyapatite from thua thien hue’s cockle shells
... Reinforcement as Bone Graft Substitute, Academic dissertation, University of Helsinki 68 38 Pierre Layrolle, Atsuo Ito, Tetsuya Tateishi (1998), “Sol-Gel Synthesis of Amorphous Calcium Phosphate ... Biomatarials, p.96-109 41 Santhosh S. , Balasivanandha Prabu S (2012), Synthesis and characterisation of nanocrystalline hydroxyapatite from sea shells, Vol.2, Issue 3-4 42 Satou K., Guicciardi S. , ... Amin Shavandi, Alaa El-Din A Bekhit, Azam Ali, Zhifa Sun (2015), Synthesis of nano-hydroxyapatite (nHA) from waste mussel shellsusing a rapid microwave method”,Materials Chemistry and Physics,Vol.149150,...
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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc
... concentration of CoA (Fig 2B) Although CoA was an essential substrate of this assay, pyruvate synthesis by POR was inhibited in the presence of excess CoA, which caused the reverse reaction (oxidative ... Ms Ayako Nishiyama for the preparation of the recombinant LDH; and Dr Ki-Seok Yoon (Ibaraki University) for helpful discussions This research was supported in part by Grants-in-Aid for Scientific ... to increase sensitivity because the amount of the radical intermediate was much less than for (A) Pyruvate synthesis by pyruvate oxidoreductase dized [3Fe- 4S] 1+ signal in Fig 3A disappeared in...
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Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... carcinogenesis In normal conditions, the expression of KLF4 mRNA is most abundant in the colon and skin in mice, whereas expression of KLF4 is decreased in intestinal adenomas of multiple intestinal ... cDNA using the First Strand Synthesis Kit (Invitrogen) The cDNA from this synthesis was then used in quantitative real-time PCR analysis with the TaqMan system (ABI-Prism 7700 Sequence Detection ... chosen as the stimulus in the subsequent experiments The expression of SR-BI was also investigated in PBMC and THP-1 cells As shown in Fig 1C,D, oxLDL decreased the expression levels of SR-BI, and...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... P IV) In vivo toxicity Protein synthesis (% of control) The addition of pulchellin isoforms to cultures of HeLa cells resulted in high inhibition of protein synthesis (Fig 4) The IC50 values showed ... chains of ricin, a toxic protein inhibiting protein synthesis Biochemistry 12, 3121–3126 21 Olsnes S & Pihl A (1973) Isolation and properties of abrin: a toxic protein inhibiting protein synthesis ... (P II), and domain comprises residues 388–514 (P I) , 389–515 (P II) or 388–513 (P III and P IV) B-chain A-chain P P P P P P I · P II III · P IV I · P III I · P IV II · P III II · P IV Domain...
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Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx
... is not a major site of phosphorylation Discussion In this study, the effects of TDI-inducing missense mutations on receptor processing, endocytosis and ubiquitylation were investigated by using ... Interestingly, whereas TDII is exclusively accounted for by a single recurrent K650E missense mutation in the TK2 domain, TDI has been ascribed to a series of mutations creating cysteine residues ... treated with sulfo-NHS -S- S-biotin, a previously described procedure for analysis of endocytosis was used [36] Briefly, after biotinylation of cell surface proteins, excess biotin was quenched by incubating...
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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx
... ratio in VsEndA is found In addition, there is a slight decrease in D + E The difference in pI reflects this substitution of charged residues, by being one unit higher for VsEndA VsEndA also binds ... the amino acids of VsEndA and VcEndA Numbers indicate cysteines involved in disulfide bridges; stars indicate Mg2+-co-ordinating residues, triangles indicate the catalytically important His80 and ... ssDNA and plasmid were analyzed using linearized pBAD ⁄ gIII plasmid, linearized and denatured plasmid and intact plasmid The pBAD ⁄ gIII plasmid was linearized using SalI and denatured by incubation...
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Tài liệu The History Of England, Volume I, Part Viby From Charles Ii To James Ii (illustrated Edition) (dodo Press) By David Hume ppt
... expressed a desire of finding expedients still to retain them But his wise minister set before him the dangerous spirit by which these troops were actuated, their enthusiastic genius, their habits ... most guilty of his enemies, and left hopes of favor to his most violent opponents From the whole tenor of his actions and discourse, he seemed desirous of losing the memory of past animosities, ... high court of justice were disabled from bearing offices These were all the severities which followed such furious civil wars and convulsions The next business was the settlement of the king’s...
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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... despite the substantial sequence similarities as indicated by six distinct regions with conserved amino acids [26] For instance, LepB from Escherichia coli is 323 amino acids long and exists only ... also as a sodium and potassium adduct ion (Fig 3) Interestingly, after collision induced dissociation of these peptide ions, only y-fragments were observed The sodium and potassium ions, respectively, ... sequencing using matrixassisted laser desorption ⁄ ionization time of flight mass spectrometry (MALDI-TOF-MS) with chemical assisted fragmentation (CAF) [30] We modified this method in a way so that...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... properties of chitinase A from Vibrio carchariae length of chitooligomers implies that the binding site must be composed of an array of subsites, probably six GlcNAc subsites This corresponds to ... chitin and is active as a monomer of Mr 62 700 Analysis of chitin hydrolysis by using the viscosity assay and HPLC-ESI MS suggested that the newly isolated chitinase acts as an endochitinase [25] ... systematically investigated, by using ESI MS, the kinetic properties of chitinase A with pNPglycosides, chitooligosaccharides, and chitin The initial velocity of the enzyme for concentrations...
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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... paralogous Sip proteins is also indicated from phenetic distance analysis as illustrated in Fig As much as 23 Bacillus Sip proteins known from B subtilis, B amyloliquefaciens, B licheniformis, B stearothermophilus, ... subtilis SipV-, SipS,T,U- and B anthracis Sip3,5-like SPases, respectively This analysis showed close relationship between Sip proteins of B amyloliquefaciens and B subtilis as well as their relatedness...
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Báo cáo khoa học: A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efficient catalysis doc
... where it alleviates zinc inhibition This is entirely consistent with previous studies demonstrating that the antenna is necessary for GTP inhibition [33] and from naturally occurring mutations in ... abrogates Zn2+ inhibition when glutamate is used as substrate, but does not compete directly with its binding [25] Discussion Fig Binding environments of the Zn2+ atoms near the GTP binding site ... vicinity of this zinc binding site It was proposed that the drugs act by affecting the protein dynamics necessary for catalysis and it seems likely that zinc does the same [40,42] Fig Overview...
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Báo cáo khoa học: Heterologous synthesis of cytochrome c¢ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery ppt
... protein in terms of biogenesis, which is different from System I cytochrome c biogenesis The above EC b562 variant with the c -type heme-binding Cys–X–X–Cys–His motif was further modified so as to ... Fig Heterologous synthesis of cytochromes c by Escherichia coli strains Periplasmic extracts (equivalent to · 108 cells) of the E coli RI242 and RI89 strains, and the JCB387 strain without (indicated ... c552 protein This is possibly a result of the shortage of Ccm proteins, because the expression of ccm genes is repressed under aerobic growth conditions In the presence of the pEC86 plasmid, both...
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