rnai screening for host factors involved in viral infection using drosophila cells
Molecular biology and pathogenesis of coronavirus virus and host factors involved in viral RNA transcription
... Amino acid residues critical for RNA-binding in the N-terminal domain of the nucleocapsid protein are essential determinants for the infectivity of coronavirus in cultured cells Nucleic Acids Res, ... the virus particle onto host cells via their attachment protein, S, the receptor binding protein S protein has two functional domains, the receptor binding domain, which defines the tropism of the ... synthesis Zinc finger CCHC-type and RNA binding motif (ZCRB1 alias MADP1) has been identified as a possible host protein involved in RNA synthesis of coronaviruses The protein has found to interact...
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SCREENING FOR DIFFERENTIALLY EXPRESSED GENES IN DENGUE INFECTION UNDER ANTIBODY DEPENDENT ENHANCEMENT CONDITIONS
... Mitochondria antiviral signaling protein MDA5 Interferon-induced helicase C domain-containing protein miRISC RNA-Induced Silencing Complex loaded with miRNA miRNA Micro RNA MOI Multiplicity of infection ... capable of inducing ADE even in FcγR-/- host cells [Mady 1992] (refer Figure 1.9) Using anti-prM IgG and in the absence of complement, Huang et al manage to induce ADE in FcγR-/- host cells, and ... as it influences the baseline infection rate With a higher baseline infection rate there will be less room for enhancement Therefore, it was generally observed that high multiplicity of infections...
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Báo cáo y học: "A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells" doc
... essential for the interaction between retroviruses and ES cells In previous studies, several host genes required for viral infection were identified by screening a gene trap library constructed in somatic ... screens in vitro by identifying mCat-1 as a critical gene in ES cells that is required for retroviral infection This screen was conducted by superinfection with a retroviral vector carrying the ... so that every single infectable cell in the culture has at least one infection event In practice, the need to infect every cell in a culture of 109 cells requires superinfection, in which every...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... domains, including an N-terminal STAT protein interaction domain, which strengthens interactions between STAT dimers on adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain,...
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Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
... difficulty in the successful expression of molybdoenzymes in the xanthine oxidoreductase family The most important factor involved in producing a functional MPT center in recombinant XDH, using the ... performed using the dithiol method [30], while iron analysis was performed by using the modified ferrozine complex method [31] Metal analysis of the recombinant protein samples were also performed ... proteins, which are involved in Fe–S cluster biogenesis [20] A similar protein, ycbX, also exists in E coli [16], suggesting that this bacterium has the machinery to sulfurate the MPT in a recombinant...
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Báo cáo lâm nghiệp: "Screening for efficient cold hardening in a breeding population of Salix using near infrared reflectance spectroscopy" pps
... Douglas fir [1] In conclusion, the spectral method for determining cold hardiness is best applied to intact tissues undergoing initial cold hardening, and by utilising information in both the visible ... capacity for cold hardening in relation to prevailing winter temperature minima [9] Similarly, a greater genetic variation in cold hardiness in the autumn than in the winter has been observed in sessile ... hardening-related information: in the near infrared range, they revealed the decreasing relative water content with advancing cold hardening, and, in the visible range, they revealed the decreasing...
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Báo cáo khoa học: "Factors involved in Pinus radiata D. Don. micrografting" pot
... text for definition of plant code) Different letters for the same variable indicate significant differences (χ2 test with P < 0.05) In Pinus radiata high concentrations of auxins and cytokinins ... micrografting conditions, we studied effect of true age on grafting success (figure 7) In vitro establishment ability using micrografting depends on the tree age since outgrowth decreases during ageing ... cytokinins were required for early development of the micrograft in vitro This differed from Sequoia, in which exogenous gibberelin and cytokinins not influence the reinvigoration effect of the...
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báo cáo khoa học: " WRKY Transcription Factors Involved in Activation of SA Biosynthesis Genes" doc
... mutations as indicated in panel B Plus signs above the lanes indicate binding mixtures containing 0.5 μg recombinant GST/WRKY28 Minus signs above the lanes indicate binding mixtures without recombinant ... WRKY28 binding sites indicated against a grey background A schematic representation of the fragments tested in EMSAs for binding WRKY28 is given in Figure 5B Figure 5C shows the consensus binding ... important for binding Changing CT to TC resulted in a binding of WRKY28 that was as strong as to the wild type sequence (m2.5, Figure 4A, Lane 18) Changing CT to TT significantly lowered binding (m2.6,...
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báo cáo khoa học: " Dissection of genetic and environmental factors involved in tomato organoleptic quality" doc
... should be identified and transferred into breeding lines The emerging information on gene expression profiling during fruit ripening provides a basis for connecting genes and regulators with biochemical ... sourness In general, all the ecotypes grown in Ercolano were given the lowest attribute intensity, those grown in Sorrento intermediate intensity and those in Sarno the highest intensity, for all ... of information, including phenotypic, biochemical and molecular data, on traditional tomato ecotypes that could constitute the basis to elucidate which biochemical factors are mainly involved in...
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báo cáo khoa học: " DNA polymorphisms and haplotype patterns of transcription factors involved in barley endosperm development are associated with key agronomic traits" pot
... conserved cis-acting element, which contains two distinct protein binding sites: the prolamin-box (PB) and the GCN4-like motif (GLM) Four transcription factors (TFs) are the gibberellin-regulated ... grown in the greenhouse and leaves from 2-week-old seedlings were harvested and bulked for genomic DNA extraction using the method described in Stein et al [20] PCR-primers were designed using ... are given in additional file PCR for single nucleotide polymorphism (SNP) analysis by DNA sequencing was performed as described in full detail by [22] In preparation for DNA sequencing, we purified...
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Báo cáo khoa học: "Screening for several potential pathogens in feral pigeons (Columba livia) in Madrid" doc
... 2007: 62 individuals were captured in November 2006, 27 in May 2007 and 29 in November 2007 All pigeons were ringed for identification in case they were captured again in later samplings Pigeons ... Campylobacter coli for all three sampling times This echoes previous findings in the literature, such as the study carried out in Barcelona [23], in which no trace of C coli was found Analysing Chlamydophila ... parameters due to the stress induced by manipulation of the pigeon Blood (0.5 ml) was extracted into tubes containing EDTA as anticoagulant Cloacal samples were obtained by introducing 0.5 ml of sterile...
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Báo cáo y học: " Patterns of evolution of host proteins involved in retroviral pathogenesis" potx
... isomerase), is incorporated into HIV-1 particles through an interaction with the viral capsid [10] Cyclophilin A is incorporated only into viral particles of viruses of the HIV-1M/SIVCPZ lineage, where ... selection in the TRIM19 (PML) gene suggests that its encoded protein does not have antiviral activity, or that the protein acts as an intermediary, lacking a physical protein-protein interaction ... protein has no significant effect Various roles have been proposed for TRIM19 (PML) in retroviral replication [8,25], although these findings remain controversial [26] Many other viruses, including...
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Báo cáo y học: "A novel scheme to assess factors involved in the reproducibility of DNA-microarray data" pdf
... accommodated in the VG interaction, but rather in the residual error The remaining 14.9 % variance in the VSG interaction still contains the Gene × Array interactions for S1 (7.8 %) and sampling differences ... variance in the VG interaction remaining virtually unchanged By combining S2 and S3, the Gene × Array interactions occurring specifically on S2 and S3 are pooled They are, thus, not accommodated in ... determined for in studies from Chen et al and Dombrowski et al [18,23] The latter study describes the use of a direct labeling kit In contrast, indirect labeling was used in our study, in which...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... Non-silencing Rnai Lef Rnai Lef Rnai Lef Rnai Dot 1l Rnai Dot 1l Rnai Catnb Rnai Catnb Rnai Catnb Rnai Catnb Rnai Catna1 Rnai Catna1 Rnai Catna1 Rnai Zfx Rnai Zfx Rnai Zfx Rnai Zfx Rnai lefty2 rnai ... Rnai lefty2 rnai lefty2 rnai lefty2 rnai Lefty1 RNAi Lefty1 RNAi Lefty1 RNAi Lefty1 RNAi Gata3 RNAi Gata3 RNAi Gata3 RNAi Gata3 RNAi Rex Rnai Rex Rnai Rex Rnai Rex Rnai Oct4 Rnai Luciferase activity ... 2004) 42 Using these criteria, 70 candidate genes were shortlisted An RNAi screen in mouse ES cells (E14) using OCT4 and Nanog promoter activity as readouts was then performed in joint effort with...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
... manner in vitro is addressed Interestingly, it was found that Nodal signaling is able to arbitrate at least three distinct fate decisions in ES cells Perturbation of endogenous signaling in ES cells ... elicit effects in embryos in a dose dependent manner In this thesis, the possibility that Nodal signaling might, as in the in vivo scenario, likewise influence ES cell fate decision in a dose dependent ... the individual contribution of SMAD2 and SMAD3 towards the various cell fate arbitrated by Nodal signaling Here, data showing that inhibition of SMAD3 activity blocks Nodal/Activin signaling induced...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... (See appendix for components) Briefly, the cells were washed three times with PBS prior to fixing in 4% formaldehyde for 1min Before the stain solution was added, the cells were again rinsed thrice ... Protein extraction and western blotting To obtain protein extracts, cells were trypsinized from culture dishes harvested in chilled PBS, centrifuged at 10,000g for at °C, washed again in PBS and incubated ... experiment The E14 cells were fed daily with medium containing fresh Activin A 4.14.2 Inhibition of Nodal/Activin signaling To abolish/diminish Nodal/Activin signaling, the cells were treated...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
... Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells Cell 113, 631642 Moffat, J., and Sabatini, D M (2006) Building mammalian signalling pathways with RNAi screens Nat ... (2007) Activin-Nodal signaling is involved in propagation of mouse embryonic stem cells J Cell Sci 120, 55-65 Oh, S P., and Li, E (1997) The signaling pathway mediated by the type IIB activin receptor ... patterning and lateral asymmetry in the mouse Genes Dev 11, 1812-1826 Pease, S., and Williams, R L (1990) Formation of germ-line chimeras from embryonic stem cells maintained with recombinant...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... which the Lefty proteins can inhibit Nodal signaling using in vitro assays They showed that Lefty proteins can interact with Nodal proteins in solution and block Nodal from binding its receptors ... of the HMG-domain DNA binding protein family It has been demonstrated that Sox2 has a role to play in maintaining epiblast pluripotency, as gene targeting used to inactivate Sox2 in the mouse causes ... been shown that 50ng/ml recombinant Activin A, in combination with 50ng/ml FGF7 and 10nM nicotinamide can maintain human ES cells cultured on laminin substrate in the absence of feeder layers...
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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... Member of TGFsuperfamily Involved in Nodal signaling Nodal antagonist • • • • • Rex2 • Zinc finger protein • • • Zfx • • • a-catenin • Zinc finger protein Transcription factor Link to canonical Wnt ... -catenin (Catnb) Dot1l Lef1 Gata3 • • • • • • • • complex Involved in Wnt signaling Subunit of Cadherin protein complex Intracellular mediator of canonical Wnt signaling Non-SET domain protein Histone ... Nature/Function/Pathway(s) involved Lefty1 • • • • Secreted protein Member of TGFsuperfamily Involved in Nodal signaling Nodal antagonist Criteria met • • • • High expression in ES cells and ICM Differential...
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báo cáo hóa học: " Nogo receptor is involved in the adhesion of dendritic cells to myelin" ppt
... Journal of Neuroinflammation 2011, 8:113 http://www.jneuroinflammation.com/content/8/1/113 repeat and Ig domain-containing, Nogo receptor-interacting protein) [12] However, due to the findings of NgR1 ... used per staining Each staining consisted of a fluorescein isothiocyanate (FITC)-labelled antibody and phycoerythrin (PE)-labelled antibody, occasionally in combination with a peridinin chlorophyll ... incubated for 20 minutes at room temperature in the dark Cells were washed and resuspended in 300 μl Cell Wash before being analysed by flow cytometry with a BD FACScan instrument using Cell Quest...
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