restricted invariant nkt cells inkt cells type i nkt cells
báo cáo hóa học:" KSP inhibitor ARRY-520 as a substitute for Paclitaxel in Type I ovarian cancer cells" potx
... apoptosis in Type II EOC cells To determine whether the decrease in cell viability is due to the induction of apoptosis, we measured caspase activity in ARRY-520-treated Type II EOC cells Following ... reached in either compound in Type I EOC cells Figure II EOC cells ARRY-520 significantly decreases the number of viable Type ARRY-520 significantly decreases the number of viable Type II EOC cells ... would be beneficial for the treatment of ovarian cancer patients ARRY-520 is an inhibitor of the mitotic kinesin, KSP KSP inhibition prevents bipolar spindle formation leading to mitotic arrest and...
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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
... chondrogenic differentiation was confirmed using standard methods described below Osteogenic differentiation Osteogenic differentiation of stem cells using an osteogenesis kit (Chemicon International, ... (Allegiance, McGaw Park, IL) Immunohistochemical localisation of CD markers, types I and II collagen, chondroitin sulphate, decorin and keratin sulphate utilised antibodies used techniques described ... (AAPER, Shelbyville, KY) until processed for paraffin embedding The CD antibodies work to identify cells by immunohistochemical visualisation CD surface marker identification, along with Page of...
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Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf
... trigger similar host IFN-related antiviral responses Type III IFNs, were more prominently induced by both viruses when compared with type I IFNs This highlights the significance of type III IFN ... up-regulation of the type III IFNs, IL28A, IL28B and IL29 following seasonal H1N1 or pdmH1N1 infection Although type I and type III IFNs bind to distinct receptors, they elicit similar intracellular signals ... experiments Virus infection of type I- like alveolar epithelial cells Type I- like alveolar epithelial cells were infected with pdmH1N1 and seasonal H1N1 at a multiplicity of infection (MOI) of two Minimum...
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Báo cáo y học: " Inhibitor of IκB kinase activity, BAY 11-7082, interferes with interferon regulatory factor 7 nuclear translocation and type I interferon production by plasmacytoid dendritic cells" ppsx
... secretion is not impaired in mice deficient in TBK1 or IKKτ [7], indicating that these two IKKs are dispensable for TLR-mediated induction of IFN-α in pDCs Similar to IKKα deficiency, IRAK1 deficiency ... production under in vitro experiments BAY11 inhibits inducible IFN-α production in vivo Finally, to weigh up the possibility of inhibiting type I IFN production through BAY11 therapy in SLE, ... a more plausible explanation for its inhibitory activities in regards to both IFNα and TNF-α in pDCs is that BAY11 targets IKKα in the inhibition of IFN-α and IKKβ in the inhibition of TNF-α...
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Báo cáo y học: "CD4+ T cells spontaneously producing human immunodeficiency virus type I in breast milk from women with or without antiretroviral drugs" potx
... HIV-1 RNA levels were similar between aviremic and viremic individuals in breast milk-derived cells but were lower in blood-derived cells from aviremic individuals by comparison with viremic individuals ... of infective virus from the HIV-1 cell reservoir in women receiving protease inhibitor-containing regimens By contrast, while most reverse transcriptase inhibitors have a good bioavailability in ... HIV-1 RNA in the blood of aviremic individuals suggests a residual virus replication despite undetectable HIV-1 plasma viral load This observation is in agreement with data clearly indicating that...
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Báo cáo y học: "Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules" ppt
... infection might have induced apoptosis of infected cells, hence, elimination of the infected cells [19] In this study, HTLV -I Tax was detected in lung epithelial cells from patients with HTLV -I- related ... higher in A549 cells cocultured with MT-2 cells Thus, consistent with the ability of HTLV -I to induce transcription of several cellular genes, infection of lung epithelial cells with HTLV -I increased ... lymphotropic virus type I (HTLV -I) proviral DNA load in HTLV -I carriers Thorax 2005, 60:138-143 Crandall ED, Kim KJ: Alveolar epithelial barrier properties In The Lung: Scientific Foundation Edited...
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Báo cáo y học: "Microarray study reveals that HIV-1 induces rapid type-I interferon-dependent p53 mRNA up-regulation in human primary CD4+ T cells" doc
... complete inhibition of the HIV-1-mediated increase in p53 gene expression in presence of a combination of blocking antibodies specific for type- I IFN (i. e IFN-α and IFN-β) This clearly establishes ... http://www.retrovirology.com/content/6/1/5 ing or bearing host-derived ICAM-1 were found to be comparable Figure with anti-IFN-α and IFN secretion Virus-induced type- I -β antibodies and blocking experiments Virus-induced type- I IFN ... providing new insights into the intricate interactions occurring during infection Nevertheless, there is still a paucity of data regarding the modifications in gene expression profiles induced...
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Báo cáo y học: "Two discrete events, human T-cell leukemia virus type I Tax oncoprotein expression and a separate stress stimulus, are required for induction of apoptosis in T-cells" pptx
... levels Y-axis is % apoptosis, and X-axis is hours after treatment B) and C) Cell viability was quantified using a modified MTT colorimetric assay Quantification of viability in HTLV -I transformed ... ell V iability R atio (treated / untreated) 1.2 Figure Quantitation of apoptosis and viability in Jurkat and JPX-9 cells treated with ZnCl2 or CdCl2 Quantitation of apoptosis and viability in Jurkat ... transforming protein, it seems unlikely that this oncoprotein's primary function is to induce apoptosis Here, we show that Tax-alone, consistent with its oncogenic role, is insufficient to induce...
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Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... endothelial activation in response to proinflammatory stimuli [2] Overexpression of KLF4 in J774a macrophages induced the macrophage activation marker inducible nitric oxide synthase and inhibited ... conditions, the expression of KLF4 mRNA is most abundant in the colon and skin in mice, whereas expression of KLF4 is decreased in intestinal adenomas of multiple intestinal neoplasia mice and in ... T Yang et al Fig DNA binding activity and transcription activity of KLF4 to the KLF binding element of hSR-BI promoter in PMA-differentiated THP-1 macrophages (A) Binding activity of KLF4 to...
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Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx
... immunoprecipitated with an anti-FGFR3 serum then immunoblotted with anti-ubiquitin and anti-FGFR3 sera (D) Disabling the c-Cbl ubiquitylating activity does not affect the ubiquitylation of the wild -type, ... with chloroquine as indicated Lysates were immunoprecipitated and processed for immunoblotting with anti-ubiquitin and anti-FGFR3 sera (C) Ubiquitylation of the wild -type receptor is increased by ... FGFR signals via different signalling pathways resulting in the regulation of many cellular processes including proliferation, differentiation, migration and survival [1–4] Dominant mutations in...
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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... substantial sequence similarities as indicated by six distinct regions with conserved amino acids [26] For instance, LepB from Escherichia coli is 323 amino acids long and exists only as a single ... analyzed by ESI-QTOF MS (Fig 3) in the positive ion mode, to get high quality fragmentation pattern for peptide sequencing Fifteen 2893 Signal peptidase I activity in M pneumoniae I Catrein et al ... protein, additional information about post-translational modifications, like 2895 Signal peptidase I activity in M pneumoniae Fig SIGNAL P predicted and experimentally verified cleavage site for P40...
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Tài liệu Báo cáo khoa học: Type I antifreeze proteins expressed in snailfish skin are identical to their plasma counterparts doc
... Expression of snailfish type I AFPs in skin tissue A B C D Fig Distribution of type I AFP mRNA in skin and liver tissues from Atlantic and dusky snailfish (A) Northern blot analysis of skin tissue ... the winter It is possible that different physiological or environmental cues initiate expression in each tissue separately Previous studies have shown that type I AFP expression in liver is seasonally ... previous northern blots At least nine individual fragments can be distinguished when Atlantic snailfish DNA is digested with HindIII (Fig 5; lane 3), whereas up to 10 are visible for dusky snailfish...
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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... cut with either PstI and EcoRI in case of sipV or EcoRI-SacI in case of sipW and ligated into corresponding sites of pUC18 DNA The ligation mixes were used for PCR with oligo nucleotides Uni1 or ... B subtilis, respectively (Fig 1,B3) This map position also indicated the B amyloliquefaciens sipW(ba) gene to be similar to sipW(bs) of B subtilis [12] Abundance of sipW-encoding DNA in diverse...
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Báo cáo khoa học: Enzymatic investigation of the Staphylococcus aureus type I signal peptidase SpsB – implications for the search for novel antibiotics ppt
... inhibitor, v0 is the initial velocity in the absence of inhibitor but with (2%) dimethylsulfoxide The IC50 value was determined by tting the percent inhibition versus inhibitor concentration using ... concentration) Percent inhibition was calculated using the equation [(1 (vi v0)] ã 100, where vi is the initial ab ỵ cxd b ỵ xd 2ị Activity at varying pH For determination of optimum pH, the in vitro ... mechanism of bacterial type I signal peptidases a novel antibiotic target Pharmacol Ther 87, 2749 Sung M & Dalbey RE (1992) Identication of potential active-site residues in the Escherichia coli...
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Báo cáo khoa học: Involvement of lysine 1047 in type I collagen-mediated activation of polymorphonuclear neutrophils doc
... (contained within the cyclic peptide CTRKKHDNAQC derived from jararhagin disintegrin) is essential for binding to the I domain of a2 integrins [24] Similarly, members of the ‘lysine–threonine–serine ... competitive agent of the interaction between collagen and PMNs because it induced a dose-dependent inhibition of ROS production, indicating the impairment of the interaction This inhibitory effect ... Biochim Biophys Acta 1764, 1730–1734 Wang H, Yu A, Wiman B & Pap S (2003) Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with...
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Báo cáo khoa học: Promoters of type I interferon genes from Atlantic salmon contain two main regulatory regions docx
... This indicates that PR -I is most important for poly (I: C) induction in these cells In TO cells, all constructs showed similar levels of relative luciferase activity after stimulation with poly (I: C) ... proximal PR -I is most activated upon poly (I: C) induction, at least in nonimmune cells, producing high amounts of short IFN transcripts, while the distal PR-II is poorly induced in TO cells Discussion ... shown (Fig 7) PDTC is a metal chelator with antioxidant properties which specifically inhibits NFjB-induced pathways [39] Secondly, partial inhibition of promoter activity by 2-AP (Fig 7), indicates...
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Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx
... Interaction with wild -type decorin core protein (obtained by chondroitin ABC lyase digestion) in HBS Decorin core protein concentrations were as indicated (B) Interaction with wild -type decorin in ... chips and with 0.3 M NaCl in the case of immobilized type VI collagen To form a complex consisting of decorin, type I and type VI collagen, type VI collagen was first immobilized After binding ... decorin significantly increases the binding affinity between the two collagens (Table 4) Discussion In this study we investigated the interaction of wild -type decorin and decorin mutants with type I...
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... in arginase I [9,23,24] On this basis, direct repulsion of the negatively charged Asp130 would explain the increased Km value for arginine inhibition and Ki value for ornithine inhibition of ... respectively The Table Kinetic properties of the wild -type and N130D variants of human arginase I The inhibitors used were ornithine (orn) and guanidinium chloride (Gdn) Substrate Arginine Agmatine ... arginase I exhibited no preferential specificity for arginine over agmatine In addition to supporting a critical role for Asn130 in arginase I, our present results further substantiate the existence...
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Báo cáo khoa học: Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry doc
... pretreated with NPDS, and after digestion incubated with propionyl-CoA The identity of domains present in each peak is indicated, together with their inferred acylation status Separation conditions ... providing evidence that domains of type I PKS retain their intrinsic activity after cleavage of their linkers It is significant that KS1 was still observed fully acylated after acylation of digested ... investigation of intermediates in the chain extension process on type I modular PKS proteins The information provided by such studies should be particularly useful in optimizing the efficiency...
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Báo cáo Y học: Importin a binds to an unusual bipartite nuclear localization signal in the heterogeneous ribonucleoprotein type I pptx
... accumulation and reduces importin a binding This conclusion fits with the in vitro binding studies, which showed that the NLD -I domain binds strongly to importin a and the binding is diminished or eliminated ... displays significant binding to importin a, which is diminished or eliminated by mutations in both basic stretches The present data support the model for the recognition of bipartite NLS derived ... proteins that contain the NLD -I type NLS, is an energydependent process mediated by importin a Deletions in the basic regions within the N-terminal domain of hnRNP I and nPTB inhibit nuclear import...
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