... Enhancer-Binding Proteins DNA Looping in Regulating Promoter Activities Steps of the Initiation Process Measurement of Binding and Initiation Rates Relating Abortive Initiations to Binding and Initiating ... Synthesis Binding Sites of the ara Regulatory Proteins DNA Looping and Repression of araBAD In vivo Footprinting Demonstration of Looping How AraC Protein Loops and Unloops Why Looping is Biologically ... Assay of RNA Splicing Involvement of the U1 snRNP Particle in Splicing Splicing Reactions and Complexes The Discovery of Self-Splicing RNAs A Common Mechanism for Splicing Reactions Other RNA Processing...
... occurring in Actinidia polygama actinin a minor protein constituent of muscle, found to be concentrated in both the Z line and the I band Two components of actinin have been identified: a-actinin, ... a-actinin, F-actin cross-linking protein, a dimer of ∼200 kDa with an action similar to that of actinogelin.; and b-actinin, a dimer of ∼70 kDa, similar in action to gelsolin actinogelin a protein factor ... studies of complexes of actinomycin D and DNA suggest that the phenoxazine ring of actinomycin is intercalated between neighbouring base pairs in DNA Other conformational studies indicate that actinomycin...
... [9] Following to binding of growth factors to their corresponding receptors the tyrosine kinase activity is induced and phosphorylation of specific residue(s) in the intracellular domain of receptors ... effects by binding to specific receptor on the cell surface; which most often is associated with an intrinsic tyrosine kinase activity, or by forming a complex with an intracellular tyrosine kinase ... expression of cyclins which bind CDKs and inactivate Rb Activation of Ras also induces growth inhibitory effectors including p16 and p15 Similar to p53, p16 and p15 induce G1 arrest by inhibiting the...
... role in biological research This concept has been generalize to cloning individual genes In this case, a piece of DNA containing a gene of interest is inserted into the genome of a target cell line, ... particular kind of bacteriophage (a virus that infects bacteria) that is capable of accepting inserts of 30,000 or so basepairs The difficulties in generating and maintaining collections of clones ... in uencing the production of a single gene (either encouraging or inhibiting it), while others can in uence the production of large numbers of genes at once An important group of these proteins...
... renal excretion of argininosuccinic acid; due to a deficiency of the enzyme argininosuccinate lyase (argininosuccinase) Aka argininosuccinic acidemia argininosuccimiria ARGININOSCUCCINIC ACIDURIA ... energygenerating pathway in some microorganisms; involves the conversion of arginine to ornithine and carbamoyl phosphate and use of the latter for the synthesis of ATP argininemia HYPERARGININEMIA arginine-rich ... and H4 arginine vasopressin A vasopressin molecule in which the eighth amino acid residue has been replaced by an arginine residue Abbr AVP arginine vasotocin A vasotocin molecule in which the...
... effects of advanced glycation in experimental diabetes in vivo (6) RAGE is thought to exist in a complex with lactoferrin-like protein (LF-L) The binding site for RAGE in lactoferrin is in the ... lysozyme and lactoferrin Binding of AGE-BSA to lysozyme inhibited the enzymatic and bacteriocidal activity of lysozyme, and binding of AGE-BSA to lactoferrin inhibited the bacterial agglutination and ... carbohydrate-binding protein 35, IgEbinding protein, RL-29, HL-29, and L-34) is a 30-35 kDa β-galactoside-binding S-lectin It has an important regulatory role in the immune response It binds laminin and...
... cahnodulin-binding domains from sequence analyses The amino acid sequences of a number of cahnodulin-binding proteins have been determined, and in many cases the loeations of the binding domains ... Tyrosine protein kinase Src EGFR INS.R PDGFR 267 685 1020 565 iPLA2 ~ 420 Consensus FIr' Alignment of the sequence of iPLA2fl with the ATP-bindingdomain of some of serine/threonine protein kinases ... the ATP-binding domain of protein kinases revealed that the sequence from amino acid residue 431 to 457 of iPLA2fl exhibits similarity to the region of protein kinases involved in ATP binding (Fig...
... acitnidain Actinidin Synonym of Synonym of actinidain Actinidine A monoterpenoid alkaloid occurring in Actinidia polygama Actinin An actin-binding protein found in a Z line or Z disk of striated muscle ... anti-inflammatory agent Actin-Binding Domain A structure feature found in the actin-binding protein Actidil A trade name for triprolidine hydrochloride, an antihistaminic agent Actin-Binding Proteins ... cross-linking filaments ABP-280 Abbreviation for actin-binding protein280 from Dictyostelium with an actin-binding domain similar to that in ABP-120 ABPC Abbreviation for avidin-biotin-peroxidase complex...
... part of DNA Page of IBO2012 SINGAPORE CELL & MOLECULAR BIOLOGY Q1.3 PRACTICAL TEST (2 points × = 10 points) Which of the following principles is true of separating DNA by gel electrophoresis? Indicate ... Confirmation of insertion of human DNA in a cloning plasmid (80 points) Using this technique, you are now tasked to confirm that a fragment of human DNA “X” (approximate size: 760 base pairs) has been inserted ... samples after the 20 minutes’ incubation time During this 20 minute incubation duration, answer the following questions in the Answer Sheet: Q1.2 (2 points × = 10 points) Indicate true statement(s)...
... points × = 10 points) a Q1.4 b b (20 points) Page of IBO2012 SINGAPORE PRACTICAL TEST ANSWER SHEET CELL & MOLECULAR BIOLOGY Q1.5 (10 points) Attach photograph of gel here Q1.6 (1 point × = points) ... DNA in a cloning plasmid (80 points) Q1.1 (2 points × + point × = 20 points) Tube Tube Tube 10 DNA ‘T’ (with buffer) Tube 10 10 10 RE1 (Ndel) RE2 (EcoRI) water Total Q1.2 20 (2 points × = 10 points) ... (2 points) a b c Page of IBO2012 SINGAPORE CELL & MOLECULAR BIOLOGY PRACTICAL TEST ANSWER SHEET Part B Determination of orientation by which fragment was inserted (20 points) Q1.11 (20 points)...
... [9] Following to binding of growth factors to their corresponding receptors the tyrosine kinase activity is induced and phosphorylation of specific residue(s) in the intracellular domain of receptors ... effects by binding to specific receptor on the cell surface; which most often is associated with an intrinsic tyrosine kinase activity, or by forming a complex with an intracellular tyrosine kinase ... expression of cyclins which bind CDKs and inactivate Rb Activation of Ras also induces growth inhibitory effectors including p16 and p15 Similar to p53, p16 and p15 induce G1 arrest by inhibiting the...
... Institute of Technology in Terre Haute, IN Dr Waite is President of the Rocky Mountain Bioengineering Symposium, which is the longest continually operating biomedical engineering conference in ... bond between alanine and tyrosine The acid function of the amino acid alanine condenses with the a-amine of the amino acid tyrosine to split out a molecule of water resulting in amide, or peptide ... environment The insertion of certain amino acids, such as proline, known as a–helix breakers, destroy the continuity of the a–helix because of steric inability of those amino acids to take part in helix...
... C-terminus, VelC a 434 amino acid protein of unknown function, and VosA, a 449 amino acid protein with conserved domains suggestive of its being a DNA-binding or chromatin-binding protein A conserved ... either aiding in the chromatin remodeling preceding AflR binding to the promoters of cluster genes or by directly interacting with AflR or other pathway-specific DNA-binding proteins involved in secondary ... a 574 amino acid protein with a nuclear localization domain and a possible transmembrane domain, VelB, a 361 amino acid protein lacking both of these domains, but containing a cluster of Asp residues...
... cause of cancer demonstrate lack of understanding in differentiating the role of acute and chronic inflammatory responses in preventing or inducing cancer Consequently, despite heavy public investment ... The following is a list of major interrelated immunobiological features in carcinogenesis: a Destruction of immune surveillance (loss of balance in Yin and Yang of acute inflammation) in the microenvironment ... tissues, and in insulin-dependent or insulin-independent tissues for glucose transport Tissues susceptibility in immune-responsive, immune-privileged, insulin-dependent or insulin-independent...
... occurring in Actinidia polygama actinin a minor protein constituent of muscle, found to be concentrated in both the Z line and the I band Two components of actinin have been identified: a-actinin, ... a-actinin, F-actin cross-linking protein, a dimer of ∼200 kDa with an action similar to that of actinogelin.; and b-actinin, a dimer of ∼70 kDa, similar in action to gelsolin actinogelin a protein factor ... studies of complexes of actinomycin D and DNA suggest that the phenoxazine ring of actinomycin is intercalated between neighbouring base pairs in DNA Other conformational studies indicate that actinomycin...
... the terminal, non-reducing α-L-arabinofuranosyl groups of arabinans, arabinoxylans, and arabinogalactans A number of microorganisms including fungi, actinomycetes and other bacteria have been reported ... there are increasing number of reports on xylan binding domains (XBDs) in family 11 (family G) xylanases Xylan binding domain has been reported in endo-xylanase of Bacillus sp Strain K-1.77 The ... due to the presence of residual lignin and lignin derivatives The intensity of pulp colour is a function of the amount and chemical state of the remaining lignin To obtain pulp of very high brightness...
... β1-integrins leads also to decreased motility, whereas inhibition of αv -in tegrin causes increased motility The integrin αvβ3 plays a central role in glioma invasion Increased expression of integrin ... mainly depend on the ECMcompartment and on the cell type Different integrins are known to be involved in that proc‐ ess Integrin α5β1 binds to fibronectin, α6β1 or β4 binds to laminin, αvβ3 binds ... upregulation in gliomas and it is also in duced during periods of increased glial cell motility in development and following brain injury Gliomas express unique brevican isoforms and the processing of...
... formation In particular, increasing wood quality or modifying lignin and/or cellulose content are investigated in detail (Part II) A range of additional targets are of interest for genetic engineering ... pivotal role in increasing the understanding of the regulation of a complex biological process One common feature of these papers is their use of transgenic lines and/or mutants in combination with ... has been broad from its inception, both in terms of the sources of the libraries for sequencing and in the depth of sequencing (numbers of ESTs sequenced from a given library) As can be seen in...