... (C and D) and detected by luminescent analysis with anti-FLAG antibody (A and C) and immunoblot analysis (B and D) His-Ub or Mix-Ub indicate His-tagged ubiquitin or the mixture of FLAG-tagged and ... for screening, and the simple polyubiquitination assay was carried out with FLAG-tagged and biotinylated ubiquitins, and the crude recombinant RING-type E3s without addition of exogenous E1 and ... mixture of His-tagged and biotinylated ubiquitin E and F, Mobility shift of UPLs (E) and formation of polyubiquitin chains (F) were detected by immunoblot using anti-FLAG antibody and Alexa488-conjugated
Ngày tải lên: 12/08/2014, 03:20
... differ? Past Simple Past Simple & Present Perfect PAST SIMPLE The game finished yesterday (Time?) PRESENT PERFECT The game has finished (Time?) Past Simple & Present Perfect PAST SIMPLE PRESENT PERFECT ... Simple Past Simple & Present Perfect PAST SIMPLE The game finished yesterday (Time?) PRESENT PERFECT The game has finished (Time?) Past Simple & Present Perfect PAST SIMPLE PRESENT ... shining and the birds were singing Past Continuous Time markers - at … - this time yesterday/ last week How they relate? Past Simple Past Simple & Past Continuous When Past Simple & Past Continuous
Ngày tải lên: 06/04/2016, 14:02
Canvas Painting for Beginners pdf
... are for information purposes only and are not warranted for content, accuracy or any other implied or explicit purpose. Table of Contents Chapter 1 – Painting 101 4 Chapter 2 – Understanding ... boards, and canvas mats. Each one can be used for different styles, artwork, and even paints. Which one you use will be determined by which one you feel more comfortable painting upon. The information ... board where colors can be mixed and and different hues can be created. A before and after shot. Perspective – Being able to reproduce the same height, depth, and distance perception in a
Ngày tải lên: 31/03/2014, 14:20
DATA MINING IN BANKING AND FINANCE: A NOTE FOR BANKERS pdf
... since it helps discover information and hidden patterns from large data sets and data sources in different formats. NN and CBR techniques can be applied extensively for predicting these financial ... developments in information technology have led to huge demand for continuous analysis of resulting data. Data mining can contribute to solving business problems in banking and finance by ... the banking and insurance world is therefore the implementation of risk management systems in order to identify, measure, and control business exposure. Here credit and market risk present the
Ngày tải lên: 20/06/2014, 14:20
Báo cáo nghiên cứu nông nghiệp " Developing an Agricultural Research and Development Priority Framework for Vietnam " pdf
... evaluate policy and market impacts and other fluctuations Rural development forum helps present ideas, provide information and bring skills and knowledge to all stakeholders in agriculture and rural ... for information exchange, and help people understand policies and give feedback for policy formulation b In long run Support is provided to build community-based models of rural development and ... select and modify policy Policy website and forum transparently provide policy initiatives and solutions for comments and recommendations by stakeholders b In long run Policy analysis and economic
Ngày tải lên: 22/06/2014, 13:20
Simple present and present continuous
... Present Simple and Present Continuous By Nguyen Trong Long tronglongnguyen@yahoo.co m 09/16/13 Nguyễn Trọng Long_Vạn Ninh_ Gia Bình_ Bắc Ninh Sports and Daily activities ... N ride bikes collect things lift weights 09/16/13 go for a walk Nguyễn Trọng Long_Vạn Ninh_ Gia Bình_ Bắc Ninh We use the present simple for things that happens sometimes or all the time In this ... 9:00 a.m And close at 7:00 p.m • He works very hard He cooks in a restaurant • The Earth goes around the sun • She always reads interesting books • My mother watches horror movies To form the
Ngày tải lên: 16/09/2013, 07:10
Present perfect simple and continuous potx
... Present perfect simple and continuous They have their exits and their entrances; And one man in his time plays many parts, His acts being seven ages. At first the infant, Mewling and ... severe, and beard of formal cut, Full of wise saws and modern instances; And so he plays his part. The sixth age shifts Into the lean and slipper`d pantaloon With spectacles on nose and pouch ... soldier, Full of strange oaths, and bearded like the pard, Jealous in honour, sudden and quick in quarrel, Seeking the bubble reputation Even in the cannon`s mouth. And then the justice, In fair
Ngày tải lên: 12/07/2014, 17:20
PRESENT SIMPLE AND PRESENT CONTINUOUS - 2 ppsx
... We use We use who – who – for people for people which – which – for things for things The book is about a girl The book is about a girl who who ... Whom Whom Whom is generally used in formal contexts is generally used in formal contexts Whom Whom is used for direct objects (people) is used for direct objects (people) To To ... This is the store which which sells vegetables and fruits. sells vegetables and fruits. When When We use when for time We use when for time That was the day That was the day when
Ngày tải lên: 01/08/2014, 20:20
Ôn tập môn Tiếng Anh khối 11 - The simple present tense and the present continuous
... (1)THE SIMPLE PRESENT TENSE AND THE PRESENT CONTINUOUS 17 The simple present tense and the present continuous Put the verbs in brackets into the correct present tense Ann sees ... in your present job? I (be) there for six months And what you (do) before that? Before that I (work) for Jones and Company 18 How long you (work) for Jones and Company? I (work) them for two ... (learn) off and on for about five and a half years (Use the continuous tense) 20 I (begin) English at secondary school and (do) it for three years Then I (drop) it for a year and (forget) most
Ngày tải lên: 01/04/2021, 23:34
Bài giảng power point về thì hiện tại (present simple and present continuous)
... GOODBYE AND SEE YOU LATER [...].. .Present Continuous 1 Form (+) S am is are V-ing (-) S ‘m not isn’t aren’t V-ing (?) Wh am is are S V-ing? Present Continuous Note: Rules of V-ing Present Continuous ... a year - now - at the moment - at present - look! - listen!, - watch out! Comparison - Usages Present Simple Present Continuous Note Stative verbs DON’T form Continuous tenses Stative verbs Senses: ... TENSES Present Simple Form (+) S V(s, es) (-) S don’t doesn’t V (?) Wh does S V? Present Simple Usages - something that happens often - habits He often smokes cigars a day Present Simple Usages
Ngày tải lên: 05/04/2016, 16:15
Present perfect and present continuous perfect tense
... of continuous waiting. b. Formation The Present Perfect Continuous tense of any English verb is formed from the Present Perfect of to be, followed by the present participle of the verb. For ... that the contractions for he has, she has and it has are the same as the contractions for he is, she is and it is. See Exercise 2. 3. Spelling rules for adding ed to form the past participle ... CHAPTER 4. THE PRESENT PERFECT AND THE PRESENT PERFECT CONTINUOUS 1. Use of the present perfect The English Present Perfect tense is used to express actions
Ngày tải lên: 02/07/2014, 21:00
present perfect simple and exercise
... have sweeping has swept have swept I the floor has sweeping have landed has landed has landing They on the Moon have landing have walked has walking have walking has walked They to school ... PRESENT PERFECT GAME GO have joining have joined has joined John the army has had has having
Ngày tải lên: 30/11/2016, 14:35
the weather vocabulary and past simple and present perfect simple
... BEAUTIFULLY READ AND CIRCLE: PAST SIMPLE OR PRESENT PERFECT SIMPLE? SHE HAS LIVED/ LIVED IN LONDON FOR Y EARS SHE LIVES IN MADRID NOW SHE DIDN’T SEE/ HAVEN’T SEEN HER PARENTS FOR A LONG TIME SHE DIDN’T ... MUSIC PAINT PAINTED VAN GOGH…………… BEAUTIFULLY READ AND CIRCLE: PAST SIMPLE OR PRESENT PERFECT SIMPLE? 1 SHE HAS LIVED/ LIVED IN LONDON FOR 5 Y EARS SHE LIVES IN MADRID NOW 2 SHE... INVENT ... VASE HAS HE BROKEN A VASE?...SANDSTORM DROUGHT FLOOD TORNADO EARTHQUAKE TIDAL WAVE PAST SIMPLE READ AND WRITE ALEXANDER GRAHAM BELL GROW UP – EDIMBURGH, SCOTLAND MARRY- MABEL HUBBARD INVENT
Ngày tải lên: 06/12/2016, 15:52
Topic: THE PAST SIMPLE AND THE PRESENT PERFECT
... what simple and present perfect between past simple grammatically volunteer work and present perfect others have done signal words of past the ways to change simple and present perfect past simple ... the present perfect Language focus: Past simple tense (uses, forms, signal words) Present perfect tense (uses, forms, signal words) ed-ending pronunciation Skills: Use the past simple and present ... Interrogative) The present perfect: Signal words; Drills The differences between the past simple and the present perfect The sentence transformation between the past simple and the present perfect
Ngày tải lên: 20/08/2020, 16:49
Giáo án English 10 - The present simple tense, the present continuous tense
... past continuous tense - Ask Ss to give the form and usage of the present continuous - Ask Ss to write down the present continuous on BB - Give check and correction - Give form and usage of the present ... - Give check and correction - Give form and usage of the present continuous - Get correction C.Exercise 2: Supply the correct verb forms: The simple present tense or the present continuous tense ... The present continuous tense: - Work in pairs - Give the answers The present continuous tense: - Ask Ss to give the form and usage of the present continuous - Ask Ss to write down the present continuous
Ngày tải lên: 02/04/2021, 18:54
date of teaching lesson plan 7 date of teaching 1782009 period 1 revision a the aims and requests 1 aims help sts to review the present simple and present continous tense 2 requests by the end of t
... text and - Ss read the text and complete the list complete the list - Then ss revise the use and - Then ss revise the use and the form of the simple the form of the simple present progressive present ... again the use and the form of the - Ss say again the use and the form of the present progressive present progressive V HOMEWORK: V HOMEWORK: - Ss learn by heart the use and the form of the - ... Ss stand up and retell the Simple Present - Ss listen to T - Then answer the qs - Go to the bb and write the answer to describe the positions of the cat - Ss revise the use and the form of
Ngày tải lên: 11/04/2021, 19:19
practice test 1 practice test 1 present simple – present continuous choose the best answer 1 water at 100 degree centigrades a boil b boils c boiling d is boiling 2 george to the movies very often a
... (1)Practice test 1 (Present Simple – present Continuous) choose the best answer 1 Water at 100 degree Centigrades A boil B boils ... to Ha Long for their last summer vacation A go B are going C was going D went 3 She to brush her teeth yesterday (2)A clean and tidy B cleaned and tidied C cleaned and tidy What ... ………for a long time because it (be) ………almost ready 73.They (say ) ………they (translate)…………the text for three hours yesterday? 74 Every weekend, hundreds of Newyorkers (abandon) ………their city and
Ngày tải lên: 20/04/2021, 00:45
THE PRESENT SIMPLE AND FUTURE SIMPLE
... P1 : GRAMMAR THE PRESENT SIMPLE AND FUTURE SIMPLE (REVIEW) I THE PRESENT SIMPLE ( Thì đơn ) Cách sử dụng đơn : ✗ Diễn tả hành động , việc diễn ... nhà vào buổi tối ) II THE RUTURE SIMPLE TENSE ( Thì tương lai đơn ) CẤU TRÚC : ✗ Câu khẳng định : S + will + V(nguyên thể) VD : My mother will help me care for my babies tomorrow ✗ Câu phủ định ... tháng lần ) - Never : Không * CHÚ Ý : Từ “ba lần” trở lên sử dụng : Số đếm + times VD : She sees grandparents four times a month ( Cô đến thăm ông bà tháng lần.) * VỊ TRÍ CỦA TRẠNG TỪ CHỈ TẦN SUẤT
Ngày tải lên: 17/11/2022, 04:38