o glycomics profiling and structural analysis of mucin type o linked glycans
báo cáo khoa học: " Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L" pdf
... symbol (Def) for the locus of development funiculus [37-39] The locus has been found to be located on the bottom end of linkage group VII corresponding to chromosome no [40-42] Recently, von Stackelberg ... to their abscission [5] It has also been reported that some plants undergo floral and fruit abscission ostensibly to remove organs from the plant so that competition for pollinators and carbon ... development of strategies for crop improvement through the reduction of yield losses [23] The yield and harvestability of many agronomically important crop species have been greatly improved through...
- 7
- 372
- 0
Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... of 7.89 A between Glu183 (homologous to Glu186 of soybean b-amylase) and Glu381 (homologous to Glu380 of soybean b-amylase) of the C sepium b-amylase, fits the inverting hydrolytic mechanism of ... vacuole This particular vacuolar location of CalsepRRP not only serves as a good endogenous control for the cytoplasmic location of the C sepium b-amylase but also demonstrates that cells of C ... (b) a smaller globular region consisting of three long loops (connected to the core), and (c) a C-terminus consisting of a long loop of over 50 amino-acid residues with a seven amino-acid residue...
- 11
- 611
- 0
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... of, the oligosaccharides of BGP glycoproteins, whereas it is reported to be only a minor constituent in soybean peroxidase and horseradish peroxidase [32,33] Formation of this type of oligosaccharide ... most of which were core a-(1,3)-fucosylated, and a small portion also carries core xylosylation and ⁄ or additional GlcNAc This pattern of heterogeneity was demonstrated by MS analysis of both ... contain up to 230 proteins and about 65 of these are IgE binding proteins [20]; however, some of these are known to be either isoforms or the degraded products of allergens Furthermore, allergic...
- 10
- 665
- 0
Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf
... prior to incorporation of the three modified oxygen atoms and a cobalt atom into the model structure (C) Fo–Fc electron density map (6 r) constructed prior to incorporation of a cobalt atom into ... group was strong Moreover, two types of conformations seemed to be present in a mixed state In one state (designated as type I), one oxygen atom of the ˚ carboxylic group formed a hydrogen bond ... bTyr68 On the other hand, one carboxylic oxygen atom of another state (type II) appeared to be positioned at a ˚ short distance ( 1.4 A) from the oxygen atom of Table Kinetic parameters and cobalt...
- 10
- 510
- 0
Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx
... variants of the 90-MtCM clones resulted in inclusion bodies of the overproduced protein(s) under various conditions of growth and induction Thus, we could not experimentally verify our interpretation ... denoted as AroQc A fourth subclass of CMs denoted AroQd was proposed by Okvist et al [14], on the basis of the primary sequence alone The structural motif of AroQd CMs resembles that of AroQa, ... fluid of E coli as described for *MtCM [7] The production and purification of *YpCM was scaled up for crystallization Production and purification of selenomethionine *YpCM E coli B834(DE3), a methionine...
- 12
- 513
- 0
báo cáo khoa học: " Expression profiling and integrative analysis of the CESA/CSL superfamily in rice" doc
... Plant Biology 2010, 10:282 http://www.biomedcentral.com/1471-2229/10/282 Page of 16 Figure Unrooted tree of OsCESA/CSL protein family (A) and organization of exons and introns of the corresponding ... either OsCESA1 or OsCESA8 did not disrupt the above organization (Figure 8) Even after the double deletion of OsCESA3/OsCESA1 or OsCESA3/OsCESA8, OsCESA5 and OsCESA6 could somehow still organize ... Expression divergence of OsCESA/CSL genes in duplication We further observed the expression profiling of the duplicated OsCESA and OsCSL genes The expression of the two duplication sets OsCSLE1/OsCSLE6...
- 16
- 431
- 0
Báo cáo y học: "A systematic comparative and structural analysis of protein phosphorylation sites based on the mtcPTM database" ppt
... of conservation (percentage) 100 Figure Evolutionary conservation of phosphorylated sites Evolutionary conservation of phosphorylated sites The plots show the distribution of the percentage of ... Johnson LN: Structural mechanism for glycogen phosphorylase control by phosphorylation and AMP J Mol Biol 1991, 218:233-260 Barford D, Johnson LN: The allosteric transition of glycogen phosphorylase ... of the size and charge of the phosphate groups, their introduction could have a local, and potentially global, effect on the modified proteins This effect may translate into modulation of protein...
- 20
- 296
- 0
Báo cáo y học: "Genome-wide expression profiling and bioinformatics analysis of diurnally regulated genes in the mouse prefrontal cortex" pps
... at four time points, and hours after lights on (ZT3 and ZT9) and and hours after lights off (ZT15 and ZT21), to gain higher resolution temporal patterns of expression and to capture genes whose ... functional categories correspond to specialized aspects of metabolism and cellular responses or the regulation of these processes For example, genes involved in protein transport and localization ... http://genomebiology.com/2007/8/11/R247 Genome Biology 2007, Conclusion accessed through the National Center for Biotechnology Information Gene Expression Omnibus (GEO Series GSE9471) Our analysis demonstrates that about 10% of transcripts...
- 15
- 299
- 0
Báo cáo y học: "Sequence and structural analysis of BTB domain proteins" pps
... β-sheet formed between β1 from one monomer and β5 of the other monomer; and the packing of α1 from one monomer against α1 and the A1/A2 helical hairpin from the other monomer The strand-exchanged ... Schematic of the BTB fold topology The core elements of the BTB fold are labeled B1 to B3 for the three conserved β-strands, and A1 to A5 for the five α-helices Many families of BTB proteins are of ... Finally, T1 domains in T1-Kv proteins consist only of the core fold and associate into homotetramers [11,29] Thus, while the structures of BTB domains show good conservation in overall tertiary...
- 18
- 370
- 0
Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc
... stretches of non-homology, many of which correspond to coiled coils (Figure 2a, b) This pattern of block-by-block similarity was also observed with five other kinetochore proteins for which orthology ... transcription factor for a set of highly conserved biosynthetic genes [30], implying conservation of non-kinetochore function We therefore concentrated on components of the CBF3 complex, three of whose ... interactions Figure 11 Evolutionary development of kinetochores from yeast to mammals Evolutionary development of kinetochores from yeast to mammals (a) Model of the kinetochore using protein subunit...
- 21
- 384
- 0
Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx
... sole carbon source and monitored for the correct proliferation of peroxisomes via the analysis of cell morphology by electron microscopy We observed that both full length proteins Pex1p and Pex6p ... ProtAtag Moreover, as expression of the constructs was under the control of the endogenous promoters, overexpession was avoided, which has been shown to 49 Domain function of Pex1p and Pex6p ... functional role in peroxisome biogenesis Results The contribution of Pex1p and Pex6p to peroxisomal biogenesis is well established on the basis of the phenotypes of the corresponding null mutants Both...
- 12
- 584
- 0
Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
... conformational analysis of the inner core region of enterobacterial LPS and epitope mapping of WN1 222-5 we have in addition isolated the complete core-oligosaccharides of E coli F653 (R3-core) ... anomeric protons (Table 2) In addition, two pairs of signals originating from 3-deoxy protons of Kdo-residues were present Full assignment of proton and carbon chemical shifts and determination of 3JH,H-coupling ... by TOCSY show connectivities to protons close in space to any of these protons Therefore, more correlation signals are observed in the region of anomeric protons that resolves signal overlap, the...
- 10
- 545
- 0
Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate specificity doc
... secondary structure elements of HP-TenA are shown in the bottom line (B) Stereo view of a cartoon representation of the tetramer of HP-TenA The four chains are seen along one of the two-fold molecular ... unknown ligand was also found in TenA from P horikoshii [12] and from P furiosus [11] The flat electron density likely corresponds to an endogenous compound of the E coli where the protein was produced, ... characterized and, upon comparison with H pylori TenA, a high degree of structural similarity is observed, with the exception of mutations in position 47, from Tyr to Phe, and position 51, from Phe to Tyr...
- 9
- 491
- 0
Báo cáo khoa học: Structural and functional analysis of ataxin-2 and ataxin-3 potx
... structure of the UBX domain of the cofactor p47 (PDB identifier 1s3s) [86] Similar to the P falciparum homologue, the Cryptosporidium parvum homologue of ataxin-3 also possesses only one UIM motif ... ATP hydrolysis of VCP [157,158] This observation also indicates a close functional relationship of the homologous ubiquitin-like UBL and UBX domains Functional analysis of the Josephin domain The ... Therefore, we used this alignment of ataxin-2 to Sm1 to model the 3D structure of the Lsm domain of ataxin-2 in complex with RNA and Lsm domains of ataxin-2 protomers (Fig 3) Functional analysis of...
- 16
- 526
- 0
MECHANICAL AND METALLURGICAL ANALYSIS OF STRUCTURAL STEEL pot
... suggests a progression of failure of certain parts of the flooring in this area of the tower At the moment of collapse of WTC 2, the top portion of the building was found to have moved to the west as ... fireproofing in place 54 Figure 6–11 Missing fireproofing on flanges of exterior columns of north face of WTC Fireproofing has come off, revealing red Tnemec paint of the column (red arrows) ... Redistribution Images of WTC showed gross deformations of the south wall prior to final collapse Images taken approximately prior to collapse showed inward bowing of the exterior columns, reaching an observable...
- 184
- 361
- 1
Báo cáo hóa học: " cDNA targets improve whole blood gene expression profiling and enhance detection of pharmocodynamic biomarkers: a quantitative platform analysis" ppt
... methodologies for whole genome expression profiling from whole blood Many of these used similar methodologies for objectively measuring expression profiling performance [14,22] Others have noted ... Comparison of different isolation techniques prior gene expression profiling of blood derived cells: impact on physiological responses, on overall expression and the role of different cell types ... potential to improve sensitivity and specificity, there are remaining questions of globin impact on power to discover relevant biological signals from gene expression profiling of whole blood In order...
- 12
- 585
- 0
Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
... Correspondence: timothy.mueser@utoledo.edu Department of Chemistry, University of Toledo, Toledo OH, USA Full list of author information is available at the end of the article the caretakers of ... came from the collaborative efforts of John Kuriyan and Mike O Donnell who have completed crystal structures of several components of the E coli Pol III holoenzyme including the ψ-c complex (PDB ... proteins and protein complexes in solution These low-resolution techniques provide information about protein conformation (folded, partially folded and unfolded), aggregation, flexibility, and...
- 17
- 364
- 0
Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 1
... E47 homodimer showing the network of glutamines that were predicted to form hydrogen bonds but the distances were too far for most of them Perhaps E47 also had a positive ion in the loop coordinated ... basic-helix-loop-helix (bHLH) family of transcription factors and then focus on a special group of HLHcontaining proteins that antagonizes the function of these bHLH TFs 1.1 Classes of basic helix-loop-helix ... development, several types of experiments were performed to look at gain -of- function and loss -of- function phenotypes in different organs Genetically modified mice were generated and studied for...
- 30
- 305
- 0
Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 2
... ID2 loop region mutants ID3 loop region mutants ! ! 25! 2.3 Protein expression optimization To test for protein expression and solubility of the different expression clones, factors known to affect ... insoluble and tending to aggregate, especially at high concentrations (Colombo, et al., 2006) This could be a reason for the sparse structural information on ID2 ! 19! 1.9 Aim and Scope of Project ... using μl of protein solution mixed with μl precipitant solution and allowed to grow at room temperature, 18°C and 4°C Optimal manual setup temperature was found to be 18°C at the same conditions as...
- 19
- 292
- 0
Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 3
... were crushed and serial dilutions made from the stock solution were then used to setup the drops of µl of the diluted mixture with µl of precipitant solution and µl protein solution With increasing ... 2), flowthrough (lane 3), fractions at positions of crosses in profile from Fig 3.1C (lane 4-11) (E) Reverse affinity chromatography profile; ID2 eluted in flowthrough, residual tag bound to nickel ... 8: ID2 proteins’ expression and purification (A) Elution profile of nickel-bead affinity and desalting chromatography (B) SDS-PAGE: marker (lane 1), sample before induction (lane 2), pooled desalted...
- 5
- 253
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25