neural stem cells differentiated from embryonic stem cells proteomic identification of expressed genes
Báo cáo khoa học: Hypoxia-driven proliferation of embryonic neural stem ⁄ progenitor cells – role of hypoxia-inducible transcription factor-1a ppt
... neural stem cells derived from the embryonic mesencephalon (E13.5) were used in the present study Generally, neural stem cells were grown to form as neurospheres in vitro The ability of stem cells ... by Lipofectamine 2000, and then cells were exposed to either normoxia (20% O2) or hypoxia (10% O2) for days The percentage of GFP-positive cells was about 70% of that of total neural stem cells ... growth of most kinds of cells, promote the proliferation of some types of cells There is increasing evidence that mild hypoxia acts as a potent regulator of various types of stem cells [12] Therefore,...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... Though there are numerous reports of an extensive number of selection markers that allow for the distinction of NSCs from other neural or non -neural cells, none of the markers can exclusively identify ... Chapter 1: Introduction 1.1 Neural stem cells 1.1.1 A brief history of NSCs The field of neural stem cell (NSC) biology has its humble beginnings in the second half of the 19th century Several...
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Effects of high glucose concentrations on the expression of genes involved in proliferation and cell fate specification of mouse embryonic neural stem cells
... viability of differentiated cells from NSCs···············160 Figure Effects of HG on proliferation of differentiated cells from NSCs ········162 Figure Effects of HG on apoptosis of differentiated cells ... expression of genes involved in proliferation and cell fate specification of embryonic neural stem cells Society for Neuroscience 35th Annual Meeting, Abstract Number 24.10 (24 Neural Stem Cells in ... expression of developmental control genes in neural stem cells ···119 xxi LIST OF FIGURES Figure Mouse embryos obtained from normal and diabetic pregnancy ···········146 Figure NSCs derived from the...
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NEURAL STEM CELLS NEW PERSPECTIVES doc
... University of Turin Neuroscience Institute Cavalieri-Ottolenghi (NICO) Section Neural Stem Cells as Progenitor Cells Chapter Systems for ex-vivo Isolation and Culturing of Neural Stem Cells Simona ... Section Neural Stem Cells and Regenerative Medicine 269 Chapter 10 A Survey of the Molecular Basis for the Generation of Functional Dopaminergic Neurons from Pluripotent Stem Cells: Insights from ... through neuralization of mouse and human pluripotent cells, i.e Embryonic Stem cells derived from blastocyst stage (ESC; [11]) and reprogrammed cells (iPSC; [12, 13]), leading to the generation of...
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Neural Stem Cells and Therapy Edited by Tao Sun potx
... astroglial cells and the bona fide adult neural stem cells) , type C cells (transit-amplifying cells derived from asymmetric division of Type B cells) , type A cells (immature neuroblasts derived from ... Erkki Juronen Part Neural Stem Cells in Invertebrates 31 119 Chapter Formation of Nervous Systems and Neural Stem Cells in Ascidians 121 Kiyoshi Terakado Chapter Regeneration of Brain and Dopaminergic ... Proteins in Neural Stem/ Progenitor Cells 205 Kenichi Horisawa and Hiroshi Yanagawa Chapter 11 Active Expression of Retroelements in Neurons Differentiated from Adult Hippocampal Neural Stem Cells 223...
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neural stem cells. methods and protocols
... change a neural stem cell’s fate Under certain conditions, neural stem cells have been induced to differentiate into cells outside the expected neural lineage and conversely, stem cells from nonneural ... the differentiation of stem cells from the fetal and adult central nervous system Genes Dev 10, 3129–3140 18 O’Shea, K S (1991) Control of neurogenesis in embryonic stem cells J Cell Biol 115, ... Neural Differentiation of Embryonic Stem Cells K Sue O’Shea Introduction Differentiation of pluripotent embryonic stem (ES) cells into specific lineages is an important source of cells for implantation...
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báo cáo hóa học:" Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model" doc
... hyaluronic acid (HA)-collagen neural corRepresentative image of scanning electron microscopy of neural stem cell (NSC) at passage three derived from the neural cortex of E16 Sprague-Dawley rat embryos ... light microscopy of NSC in implanted tex of E16 Sprague-Dawley rat embryos culture on of neural stem cell (NSC) at passage three derived from the composite Representative image of scanning electron ... SP, Tsang KS, Chan AB, Lu G, Poon WS, Li K, Baum LW, Ng HK: Trophism of neural progenitor cells to embryonic stem cells: neural induction and transplantation in a mouse ischemic stroke model...
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Báo cáo toán học: "Adult neurogenesis, neuroinflammation and therapeutic potential of adult neural stem cells" ppsx
... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult-derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... neuronal cells in the adult brain originate from residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells ... plasticity of the nervous system Adult-derived neural progenitor and stem cells grafted in the brain promote neuroprotection, by an immunomodulatory mechanism Grafted neural progenitor and stem cells...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... oxygen depletion/reoxygenation conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated ... http://www.jbiomedsci.com/content/18/1/46 Page of 10 Figure Confocal microscopy of a single colony of mouse embryonic stem cells A: top view of a single slice of scanning showing most eGFP signals are ... doi:10.1186/1423-0127-18-46 Cite this article as: Jia et al.: Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells Journal of Biomedical Science 2011 18:46 Submit...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem cell as a vehicle ... marker genes between msiPSCs and MEFs 58 10 LIST OF FIGURES Figure Generation of iPS cells from mouse embryonic fibroblasts (MEFs) 55 Figure Generation of neural stem cells from ... (elaborated in section 3.1) 1.4 Stem cells 1.4.1 Adult stem cells (ASCs) Adult stem cells (ASCs), also known as somatic stem cells, are multipotent undifferentiated stem cells found in a tissue or...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... lineages of the embryo (D) Figure was adopted from Niwa (2007) 1.2 Embryonic stem cells (ESCs) Cells from the ICM can be isolated and cultured in vitro under permissive conditions as embryonic stem ... 7.2 Function of genes associated with the binding of Oct4, Sox2, Stat3 and Smad1 in mouse embryonic stem cells as annotated in NCBI xiii LIST OF FIGURES Figure 1.1 A schematic view of mouse pre-implantation ... mouse embryonic stem cells Nature Genetics 38(4):431-40 *these authors contributed equally to this work xix CHAPTER GENERAL INTRODUCTION 1.1 Stem cells Stem cells are classically defined as cells...
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A role for chondroitin sulfate proteoglycan in regulating the survival and growth of neural stem cells
... of stem cells 1.4.1 Embryonic stem cells 1.4.2 Somatic stem cells 1.4.3 Stem cells and cancer 14 1.5 Neural development 16 1.6 Neural stem cells ... 19 1.6.1 Embryonic neural stem cells 20 1.6.2 Adult neural stem cells .24 1.6.3 Neural stem cell applications .26 1.7 Methods to study neural stem cells 28 ... adopt this form of cell division to expand and replenish the depleted stem cell pool (Morrison and Kimble, 2006) 1.4 1.4.1 Types of stem cells Embryonic stem cells During embryogenesis, the ICM...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... neuroepithelial-like stem cells for the generation of modified neuronal cultures 59 4.2 Generation of gene-corrected neural stem cells from MJD patient-derived iPS cells 59 4.2.1 ... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... (Weissman, 2000) During embryonic development, a program called neurogenesis, composed of complex patterns of sequential cycles of symmetrical and asymmetrical division of neural stem cells, establishes...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
... endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of ... mesenchymal stem cells Stem Cells Dev 2005, 14:70-80 Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, Sen A, Willingmyre GD, Gimble JM: Yield of human adipose-derived adult stem cells from ... engineering by providing abundant seeding cells with high quality List of abbreviations ADSCs: adipose-derived mesenchymal stem cells; MSCs: mesenchymal stem cells; ALP: alkaline phosphatase; DT:...
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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc
... migration of mesenchymal stem cells to conditioned medium from tumor cells or bone marrow cells Stem Cells 2007, 25:520-528 Reya T, Morrison SJ, Clarke MF, et al: Stem cells, cancer, and cancer stem cells ... malignant potential of hepatic stem cells derived from the bone marrow in a mouse model of chemical hepatocarcinogenesis, their results suggested that hepatic stem cells derived from the bone marrow ... malignant phenotype of tumor cells[ 65] However, the effect of human bone marrow derived MSCs on the growth of tumoral cells is controversial HCC was thought to arise from hepatic stem cells; in their...
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Báo cáo y học: "Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing" ppsx
... and invasion of mesenchymal stem cells J Mol Cell Cardiol 2008, 44:1072-1084 30 Estrada R, Li N, Sarojini H, An J, Lee MJ, Wang E: Secretome from mesenchymal stem cells induces angiogenesis via ... dividing the number of Page of cells counted by the mean value of counted cells in controls Endothelial wound healing assay A wound healing assay was used to investigate the effects of apneic serum ... early activation of MSC: specifically, an increase in the mobility of these stem cells and in their adhesion to endothelial cells Moreover, it was also found that the serum of apneic rats improved...
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Báo cáo y học: "Stem cells and repair of lung injuries" doc
... pathophysiology of adult respiratory distress syndrome Mobilization of endogenous endothelial or epithelial stem/ progenitor cells or delivery of adult somatic stem cells, embryonic stem cells, or embryonic ... (path 1) Embryonic stem cells from the inner cell mass (path 2) or embryonic germ cells from the gonadal ridge (path 3) can be cultured and manipulated to generate cells of all three lineages cells ... organ-specific stem cells There is evidence that adult stem cells from a variety of sources can generate not only their own lineages, but those of other tissues, sometimes crossing barriers of embryonic...
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