... mixer and subjected to ultrasonication RESULTS Effects of pressure on Na+ /K+ -ATPase, Na+ -dependent ATPaseand K+ -activated phosphatase activities It could be con®rmed that the Na+ /K+ -ATPase activity, ... mL The reaction was initiated by the addition of lg enzyme + Three activities of Na /K -ATPase were determined as follows Na+ /K+ -ATPase activity was measured by monitoring the decrease in NADH ... pyruvate kinase, and 2.5 lgámL)1 LDH in a total volume of mL The reaction was initiated by an addition of 50 ng of Na+ /K+ -ATPase Na+ -dependent ATPase activity was also measured using the coupled...
... also to the activation of p42/44 MAPK (also called ERK1/2) through the Ras/Raf/ MAPK kinase/MAPK cascade (where Raf is a MAPK kinase kinase and MEK is a MAPK kinase) [29,30] However, the activation ... protein kinase C in the signal pathways that link Na+ / K+ -ATPase to ERK1/2 J Biol Chem 276, 42050–42056 32 Tian, J., Gong, X & Xie, Z (2001) Signal-transducing function of Na+ -K+ -ATPase is essential ... low K+ induction of Na, K- ATPase: Possible role of c-fos J Membr Biol 125, 163–170 10 Nakagawa, Y., Rivera, V & Larner, A.C (1992) A role forthe Na/ K- ATPase in the control of human c-fos and...
... min) The ghost cells were then incubated for h at 37 °C Samples from the ghost cells andthe supernatants were taken for scintillation counting Creatine kinase determination Creatine kinase activity ... while modifying Na/ K- ATPaseThe activity of Na/ K- ATPase was assessed by ouabainsensitive 86Rb uptake Ghost cells were incubated at 37 °C in Mops buffer and assayed at time zero and after h Resealed ... a-crystallin Na/ K- ATPase activity decreased upon incubation with fructose, H2O2 and methylglyoxal However, Na/ K- ATPase activity was preserved when the heat shock protein a-crystallin was sealed within the...
... C-terminal part of the Pdomain of the isolated H4-H5 loop (Figs and 2, Table 1) K+ activated phosphatase activities in Na+ /K+ ATPaseand H+ /K+ ATPase supposedly reect the K+ activated step of the ... experiments to the H4-H5 loop Table Eect of FITC-labeling on the phosphatase activities of Na+ / K+ ATPaseand a H4-H5 loopGST fusion protein Labeling of pig kidney Na+ /K+ ATPaseas well asthe puried ... previously forthe H4-H5 loop of pig kidney Na+ /K+ ATPase [20] The primary structure of the mouse brain Na+ /K+ ATPase from R378 to D586 was aligned with the template sequences by CLUSTALX [48] The threedimensional...
... tissues, the Na+ /K+ -ATPase is a heterodimer consisting of a 112 kDa hydrolytic a-peptide, through which the vectorial transport of Na+ and K+ takes place, andthe small a-glycoprotein (35 kDa forthe ... containing a1- as well as a2-isoforms of Na+ /K+ -ATPase the explanation forthe apparent signal-tranduction may differ [9–11] In one of the papers [9] it is mentioned that the rat a1-isoform is less ... ouabain was a theme at the 10th International Conference on Na, K- ATPaseand Related Cation Pumps in Elsinore, Denmark, in August 2002 The meeting of 250 specialists on Na+ /K+ -ATPase and related topics...
... ion Na Cl vào thể thải môi trường xác định, chế tiến hành khả thấm nước bề mặt + + thể tham gia vài enzyme Na /K ATPase mang tuyến râu hàm (antenal gland) giáp xác Hoạt tính men Na+ /K+ ATPase ... tính men Na/ KATPase mang tôm thẻ chân trắng theo thời gian độ mặn khác (chữ khác thể sai khác có ý nghĩa nghiệm thức (P
... [48] Another player in the IL-2 signaling cascades is the Jak3 kinase Jak3 -/- mice display symptoms of autoimmunity and accumulation of auto-reactive T cells in the lymphoid organs [48] It has been ... Zhernakova A, Inouye M, Wapenaar MC, Barnardo MC, Bethel G, Holmes GK, et al: A genome-wide association study for celiac disease identifies risk variants in the region harboring IL2 and IL21 Nat ... as IL-2 is first and foremost a T cell growth factor, andas such, has strong proliferative effects on all T cells, including pathogenic CD4 + and CD8+ Teff cells Forthe past decade, IL-2 has...
... ion Na Cl vào thể thải môi trường xác định, chế tiến hành khả thấm nước bề mặt + + thể tham gia vài enzyme Na /K ATPase mang tuyến râu hàm (antenal gland) giáp xác Hoạt tính men Na+ /K+ ATPase ... tính men Na/ KATPase mang tôm thẻ chân trắng theo thời gian độ mặn khác (chữ khác thể sai khác có ý nghĩa nghiệm thức (P
... transarcolemmal Na+ and K+ concentration gradients, therefore preserving membrane potential (Em) and therefore the NKA is the major focus of this thesis 2.2 Skeletal Muscle Na+ ,K+ -ATPase 2.2.1 Function and ... sodiumpotassium pump (Na+ ,K+ -ATPase or NKA) is a P-type ATPaseand is responsible forthe active transport of Na+ and K+ ions across the cell membrane against their concentration gradients and is ... is known about the NKA α3 function in skeletal muscle The β isoform performs a regulatory role in the NKA and thus contributes to NKA activity However, unlike the α isoforms, no β isoform knockout...
... pNPPase activity of Na+ ⁄ K+ -ATPase Fig Activation of pNPPase by KCl in the presence of NaCl and oligomycin The pNPPase activity was assayed in a mixture containing lg Na+ ⁄ K+ -ATPase, the standard ... ATP and 10 mm NaCl increases the affinity of pNPPase for K+ , as shown by oligomycin with NaCl (Figs and 3) The combination decreased the K0 .5 for K+ from to 0.2 mm, which was one-fifth of the K0 .5, ... with NaCl is due to the increase in K+ affinity On the other hand, Na+ occlusion was preserved under the ligand conditions used (Fig 4C) Therefore, the Na+ -occluded E1 state of Na+ ⁄ K+ -ATPase...
... confirm the name on the identification andthe name on the bond are the same, and make sure the signature on the document and on the bond compare favorably Make adequate notations about the identification ... If the difference is not obvious, ask • If the explanation makes sense, for example, married name versus maiden name, note the explanation with the signature and go to the next question • If the ... not obvious, ask • If the explanation makes sense, for example, married name versus maiden name, note the explanation with the signature and go to the next question • If the explanation doesn’t...
... S, Grandone E, Green PM, Groen HJ, Gwilliam R, Houwen RH, Hunt SE, Kaukinen K, Kelleher D, Korponay-Szabo I, Kurppa K, MacMathuna P, Mäki M, et al.: Multiple common variants for celiac disease ... to both diseases, whereas the others seemed to be disease-specific Other studies have compared and contrasted risk confirmed alleles for a variety of autoimmune diseases [9,25-27] There is clear ... include known signaling pathways (such asthe NF-κB pathway and risk of RA [33]) or catabolic pathways (such as autophagy and risk of Crohn’s disease [20]) The challenge of this compu tational approach...
... S, Adra CN, Kitaichi M, Inoue H, Yamauchi K, Tomichi N, Kurimoto F, Hamasaki N, Hopkin JM, Izuhara K, Shirakawa T, Deichmann KA: Genetic variants of IL-13 signalling and human asthma and atopy ... IL-5, and IL-13 In addition, the requirement for IL-9 in the expulsion of the intestinal parasitic nematode Nippostrongylus brasiliensis, as previously suggested in the IL-9 transgenic mice, was ... Breazeale DR, Ghosh B, Freidhoff LR, Ehrlich-Kautzky E, Schou C, Krishnaswamy G, Beaty TH: Linkage analysis of IL4 and other chromosome 5q31.1 markers and total serum immunoglobulin E concentrations...
... array Acknowledgements This work was supported by Public Health Service grant AI063982 (for GWS), AI073240 and AI63982 (for RSK), and AI063993, AI071306, and RR000168 (for DTE), andthe University ... experiments; SG, RSK, DE and GWS wrote the manuscript; and all of the authors read and approved the manuscript RSK and GWS are listed as inventors on patent filings related to the use of LMP1 and LMP1-CD40 ... 78:11715-11725 Stone GW, Barzee S, Snarsky V, Kee K, Spina CA, Yu XF, Kornbluth RS: Multimeric soluble CD40 ligand and GITR ligand as adjuvants for human immunodeficiency virus DNA vaccines J Virol 2006,...
... R4.15 tion of the binary backbone Forthe detection of the left and right T-DNA border 381 bp and 327 bp PCR products were generated using primers LB-f and pPK2-LB1 and primers pPK2-RB1 and RB-r, ... Park SY, Chi MH, Choi J, Park J, Rho HS, Kim S, Goh J, Yoo S, Choi J, Park JY, Yi M, Yang S, Kwon MJ, Han SS, Kim BR, Khang CH, Park B, Lim SE, Jung K, Kong S, Karunakaran M, Oh HS, Kim H, Kim ... computational support forthe comparison of the M grisea and F oxysporum potential pathogenicity genes Furthermore, we would like to thank Harold Lemereis, Thijs Hendrix, and Ludek Tikovsky for managing...
... of further molecular targets As DNA polymerases are essential for DNA replication and repair, their inhibition will induce cell cycle arrest at the S phase The MIC ranges for DNA polymerases in ... 24 °C for 30 min, and then centrifuged at 223 000 g for 15 Forthe assay shown in Fig 6B, there was 2% (final concentration) DMSO in all samples Supernatants and pellets were assayed forthe presence ... this reason, we focused on themolecular interactions between SQAGs andthe recombinant MCAK Kinetic parameters via surface plasmon resonance (SPR) analysis of binding between SQAG and MCAK The...
... models The determined kd was used for calculation of ka, andthe binding constants KD were determined from kd ⁄ ka We determined the binding constant KD for binding of ETBcHx to bET-1 as 6.2 ... Acknowledgements We are grateful to Clemens Glaubitz and Andreas Engel for valuable discussions, and we thank Walter Rosenthal forthe cDNA of human ETB We further ´ thank Robert Tampe and Katrin ... the relatively high mass of the receptor used as analyte is considered Ligand occlusion by immobilization on the sensor chip surface, as well as limited access to the ligand-binding site of the...
... Vulnerabilty Assesment Tool will take into account the probability of Forum on Public Policy all types of disasters, the human, property, and business impact of the event, as well asthe internal and ... When asked what the key issue was in their state or region related to emergency/BT preparedness, 82% said that there is a lack of coordination of emergency and social service networks in their ... care for those who cannot care for themselves in times of crisis and disaster Those caring forthe vulnerable elderly must better prepare themselves, their facilities, and their organizations for...
... (raises) the amount of resource elements used for data transmission as indicated in nk kj,new ) < nk kj,old ) D(Γ D(Γ k if k j,new > Γ j,old , nk kj,new ) > nk kj,old ) D(Γ D(Γ k if k j,new ... coverage On the other hand, the scenarios marked as “overall” represent the throughput obtained by the FAPs and MBS together Consequently, these scenarios show the negative effect of FAPs on passersby ... Case I ( k = and ϑ < target ), Case II ( k > and ϑ < target ), and Case III ( k > 0, target ≤ ϑ < or ϑ = 1, while not all data are sent from the FAPs to UEs due to congestion) Thetarget frame...
... with some details was put in and increase in content volume for producer and packer (ii) Separation of section for BRC – standard for packing house and packer with other sections and also upgraded ... March and April 2007 The presentation was then, used as tools in training for farmers and packers in this area 3.2 Development of quality manual The manual was first written by English andthe ... Vietnamese The manual development was based up the specific background which met to the requirements of quality systems forthe project pilot model, forthe farmer section complying to EUREPGAP and...