ligation transcription for construction of replication competent sfv vectors and libraries based on these vectors
Báo cáo khoa học: "Identification and characterisation of a novel anti-viral peptide against avian influenza virus H9N" pps
... vitro study of protein-protein interactions Construction of recombinant pC-HAt, pC-NA and pC-P1 and in vitro transcription and translation The HAt and NA gene of AIV strain H9N2 as well as the recombinant ... contrast, the control peptide CSWGEYDMC had no effect on agglutination Inhibitory effects of peptides and fusion phages on neuraminidase activity Based on the ability of the peptides and fusion phage ... inhibition of viral-induced agglutination of cRBCs by the peptides and fusion phages were monitored Twofold dilutions of untreated or peptide/ phage treated virus were incubated with cRBCs, and agglutination...
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in vitro transcription and translation protocols
... ILL of digestion buffer containing ribonuclease to each of the sample tubes and one of control tubes containing yeast RNA Add 200 l.tL of RNase digestion buffer without ribonuclease to the second ... accidental contamination of samples with ribonucleases present on the skin Three steps are required for synthesis of RNA in vitro: Preparation of the DNA template Transcription reaction Enrichment of ... solution to be convenient and fast, but you may use any conventional prehybridization and hybridization conditions normally used to screen libraries It is imperative that all candidate cDNA clones...
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Cách gieo hạt lan nẩy mầm trong điều kiện in-vitro ppt
... giống lan khác biết: Rhizoctonia repéns đặc biệt cho Cattleya, Laelie, Cypripedium Rhizoctonia mucoroides cho Vanda Phalaenopsis Rhizoctonia lanuginosa cho Oncidium, Miltonia Nấm cung cấp đường để ... nước xuất hoa lan tạo giống phong lan có màu sắc, kiểu dáng mùi thơm ngày phong phú đa dạng Do nước trồng lan xuất tập trung vào việc tạo giống phong lan nhiều cách khác gây đột biến, ... loại môi trường nuôi cấy thích hợp cho nhóm lan khác nhau: Knudson C lan Dendrobium, Cattleya MS 1/2 Hyponex (20-20-20) lan đơn thân Vanda, Phalaenopsis, đường bổ sung vào môi trường nuôi cấy 30g/l...
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báo cáo khoa học: " LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum" ppt
... analogous to that reported for mitochondria and chloroplasts, namely acylation of lysoPC of ER origin We clearly could demonstrate lysolipid acylation The concentration of lysophospholipids in isolated ... The standard incubation time was 30 and the reaction was stopped by adding 980 µl of a mixture of ice cold chloroform:methanol:water (30:60:15 by vol.) After addition of 0.5 ml 1.6 M HCl and 0.5 ... protein concentration was measured using the bicinchoninic method [48] after solubilization of membrane fractions in 0.25% Triton X-100 for 10 prior to addition of the protein determination reagent...
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Báo cáo y học: "Comparative study of serum Na+ and K+ levels in senile cataract patients and normal individuals"
... and lens membrane permeability Alteration in either of these ions leads to cation imbalance in lens which in turn results in cataract formation [36] Hence alteration in cation concentration of ... quality and different diets in nations all over the world [31,32,33] One of the proposed risk factors for cataract formation is serum Na+ level [34,35] Lens has high content of potassium and low content ... Na+, K+ of case and control groups, with a significant difference in serum + Na of case and control ones Table Comparison of Mean & SD of serum Na+, K+ in 155 patients with cataract and 155 without...
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Tài liệu Red Hat Linux Networking and System Administration P1 docx
... Australia and New Zealand; by TransQuest Publishers Pte Ltd for Singapore, Malaysia, Thailand, Indonesia, and Hong Kong; by Gotop Information Inc for Taiwan; by ICG Muse, Inc for Japan; by Intersoft for ... IN 46256 For information on licensing foreign or domestic rights, please contact our Sub-Rights Customer Care department at 212-884-5000 For information on using Hungry Minds’ products and services ... Eyrolles for France; by International Thomson Publishing for Germany, Austria, and Switzerland; by Distribuidora Cuspide for Argentina; by LR International for Brazil; by Galileo Libros for Chile;...
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Báo cáo khoa học: Selectivity of pyruvate kinase for Na+ and K+ in water/dimethylsulfoxide mixtures docx
... [32] and 1.45 mLÆmg)1Æcm)1 for lactate dehydrogenase [33] Results and discussion Comparative effect of Na+ and K+ on the activity of pyruvate kinase The effect of Na+ and K+ concentration on the ... enrichment of enzymes with the same conformation Conclusions The results of this study explain the high discrimination of pyruvate kinase for Na+ and K+ K+ and Mg2+ exhibit high selectivity for their ... of 30 and 40% dimethylsulfoxide and in the absence of ligands, pyruvate kinase exhibited the active conformation Moreover, in the presence of dimethylsulfoxide and ligands, pyruvate kinase continued...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... RGD-LCL of different concentrations (0.3125 mg/mL, 0.625 mg/mL, 1.25 mg/mL, and 2.5 mg/mL), and RGD-M-LCL of different concentrations equivalent to these RGD-LCL concentrations After exposure for ... were treated with various concentrations of RGD-LCL and RGD-M-LCL (equivalent to the same concentration of RGD-LCL) for 48h, and were again plotted as the ratio to the control group In the cells ... Encapsulation efficiency and loading-drug stability of RGD-M-LCL The peak areas for matrine in the concentration range from 1.95-250 μg/mL was linear in the HPLC assay, and the linear regression equation...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... low concentration of ethanol exerts its effect through induction of transcription factors (AP1, Elk1, Stat1, SRF and NFκB) and expression of apoptotic genes (Fas receptor, Fas ligand, FADD and ... subunit of the human negative transcription elongation factor In addition, over-expression of COBRA1 represses the transcriptional activity of activating protein-1 (AP1) transcription factor ... phosphorylation of protein kinases leads to an increased expression of transcription factors including AP1 [19], the effect of low concentration of ethanol on COBRA1 suggests a regulatory effect on transcription...
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Analysis, sequencing and in vitro expression of PCR products
... back-translation of a limited region of amino acid sequence information is shown in Figure 5.2 To reduce manipulations and avoid any contamination problems both the initial and nested PCR reactions can ... stringency conditions offer more definitive verification of product identity Some combination of approaches may be required in difficult cases Of course the most definitive confirmation of identity of a ... products and homogenous assays Southern blot and dot blot hybridization procedures allow the simultaneous detection and verification of the identity of a PCR product Once appropriate conditions for...
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Tài liệu Red Hat Linux Networking and System Administration P2 pptx
... Installing and configuring servers N Installing and configuring application software N Creating and maintaining user accounts N Backing up and restoring files N Monitoring and tuning performance N Configuring ... must be configured (Fortunately, Red Hat Linux has made this a simple and painless part of installation on all but the most obscure combinations of video card and monitor; gone are the days of anguish ... should Configuration and customization of applications is to some extent at the user’s discretion, but not entirely “Skeleton” configurations — administrator-determined default configurations —...
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Tài liệu 10 Red Hat® Linux™ Tips and Tricks pptx
... arrays for the current details, and add it to the file: mdadm -D -s >> /etc/mdadm.conf /etc/mdadm.conf should now contain something like the following: # Caution, the ARRAY and UUID should be on ... /dev/md1 and find the two devices of the array plus a hot spare, and it will move the hot spare device to /dev/md0 and begin the rebuild process Run mdadm in daemon mode and have it monitor and scan ... there are subdirectories for each process running on the system, named based on the PID number of the process In each of these directories, there is a fd/ subdirectory that contains files that represent...
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Tài liệu 7 More Red Hat® Linux™ Tips and Tricks docx
... users and groups permissions to files and directories This extends the standard Linux permissions of having one user permission, one group permission, and a permission for all others Copyright ... Extended Attributes to store the security contexts and access control lists respectively star shone brightly as it has options for backing up and restoring each of these attributes Great strides have ... Enterprise Linux now contain the acl and user_xattr default mount options, so these tar options are necessary for good backups New default mount options in Red Hat Enterprise Linux by Forrest Taylor,...
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Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
... cytosolic EF-2 on the one hand, and the mitochondrial membrane on the other hand However, the potential role (if any) of ADP-ribosylation of EF-2 in the mitochondrial apoptotic response induced ... towards cytosolic EF-2 We report on the in vivo association of ETA and ETA-A with cytosolic fractions, and the in vitro ATP-dependent and pH-dependent translocation of ETA and ETA-A from cell-free endosomes ... mobility of EF-2 ( 105 kDa) For each incubation condition, radiolabeled and nonradiolabeled EF-2 signal intensities were quantified by scanning densitometry, and the ratio of [32P]EF-2 signal ⁄ nonradiolabeled...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
... instructions Real-time PCR conditions consisted of an initial denaturation step at 95 °C for 10 min, followed by 50 cycles of denaturation for 15 s at 94 °C, annealing for 25 s at 60 °C and extension ... was added and incubated for 10 at 25 °C The reaction was stopped by the addition of lL of · SDS loading buffer containing 500 lm EDTA and boiling of the samples for prior to separation on 10% (w ... duplication experiments and 200 lgÆmL)1 for gene expression analysis The soaking concentration for S-4o9cDH-RA was 0.2–10 mgÆmL)1 for limb duplication experiments and mgÆmL)1 for gene expression analysis...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
... identifies only one region of transcriptional repression in iPLA2c and that many other regions (repression or activation) may be operative in multiple other nutritional, environmental, or cellular contexts ... upstream of exon contains a significant proportion of pre-exon promoter activity This conclusion was supported by use of a construct containing sequence 200–400 nucleotides upstream of exon (construct ... reason, this exon has been designated exon (Fig 5) Based on its location relative to other iPLA2c exons, we have designated the second new exon as exon Exon is comprised of 112 nucleotides and, ...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... Binding and replication of 3¢-end of HCV minus RNA Fig Secondary structure of RNA mutated in the SL-A1 and SL-B1 domains Only the secondary structure of the 151 nt from the 3¢-end of the minus-strand ... deletion mutants of the 3¢ terminus of the minus-strand RNA but deletions were made on the basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minusstrand RNA ... purified as previously described [17] For construction of an expression vector of FEBS Journal 272 (2005) 3872–3886 ª 2005 FEBS Binding and replication of 3¢-end of HCV minus RNA a HCV NS5B-D21 (genotype...
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Tài liệu Báo cáo khoa học: "Structural and Topical Dimensions in Multi-Task Patent Translation" ppt
... of the differences and similarities of multilingual patent data along the dimensions of textual structure and topic The second contribution is the experimental investigation of the influence of ... Mixture and pooling on text sections Table 11: Mixture and pooling on IPC sections A comparison of the results for pooling and mixture with the respective results for individual models (tables and ... translation In Proceedings of the Human Language Technology Conference and the 3rd Meeting of the North American Chapter of the Association for Computational Linguistics (HLT-NAACL’03), Edmonton, Cananda...
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