... similarity with the largest subunitof human CAF-1 (p150) [19] and the largest subunitof Saccharomyces cerevisiae CAF-1 (Cac1, chromatin assembly complex 1) [20] The sequence similarities of this clone ... ofthe CAF-1 large subunits in human, C cinerea and S cerevisiae The KER and ED domains are represented by black and grey boxes, respectively (B) Schematic diagram ofthe truncation mutants of ... lg of crude extract was loaded experiment confirmed the specific interaction of CcCac1L and CcLim15 in the crude extracts of meiotic tissues (Fig 2E) Taken together, these results suggest that the...
... maximum of 6–20% ofthe GST-CTD molecules could be phosphorylated over the course ofthe reaction It is therefore unlikely that the observed generation ofthe IIo band was a consequence of limiting ... also need to mention the mobility ofthe IIo forms ofthe different substrates The IIo form ofthe N-terminal 1–15 repeats was only slightly retarded relative to the position ofthe unphosphorylated ... possible action ofthe three kinases on the C-terminal domain (CTD) The amino acid sequence ofthe mouse CTD is shown at the bottom In the diagram, the heptad repeats, with N at position ofthe YSPTSPS...
... lack of domain B protruding out ofthe TIM barrel in the place of loop connecting the b3-strand to the a3-helix [9,21] Sharing domain B by rBATs and GH13 enzymes, and especially the sequence ofthe ... (CSRs) covering mainly the b-strands ofthe catalytic TIM barrel [3,11–13] Ofthe three GH families ofthe clan GH-H, it was the family GH13 that was originally established as the a-amylase family ... unable to reach the plasma membrane Thus, the role ofthe heavy subunit is to recognize the light subunit and to chaperone it to a proper position in the plasma membrane, i.e this subunit is not...
... those of E californicum allows the unambiguous assignment to distinct subunit types The orthologous subunits of these species share 69.1–76.2% of their amino acids, with the a subunits being the ... a-subunits and the ancestor of all other subunit types Further differentiation ofsubunit types occurred about 450–470 MYA, with the exception ofthe b/c-subunits that diverged around 420 MYA These calculations ... branches ofsubunit types d and f, and e and g The C salei hemocyanins group with the g -subunit of N inaurata, while the hemocyanin subunitofthe scorpion Androctonus australis (AauHc6) and subunit...
... for formation of short helices, transiently, and the chain as such is highly dynamic Structure ofthe core ofthe oligomers Because ofthelarge size ofthe core ofthe oligomer, the NMR spectra ... assembles in the form of rings around the necks of budding vesicles, and then a conformational change in the dynamin collar aids the scission ofthe vesicle from the parent membrane The coiled-coil ... the full length dynamin the GED interacts with the middle domain and the GTPase domain and thus the entire surface ofthe GED would not be exposed This would limit the degree of association of...
... few spores These filaments appear to me to be the disintegrated walls ofthe perithecia, and not the "filaments that bear the spores." From analogy, at any rate, the spores of all these similar ... there remains (Fig 833) a bundle of carbonous tubes, the walls ofthe perithecia Finally, these break up and disappear, leaving the upper part ofthe plant hollow The spores are elliptical, 6-7 ... homogenous gleba The species Camillea Sagraena differs from the other in having the fertile portion composed largely of spores (scanty in others) and in having part ofthe sterile portion of uncolored...
... and MS identified the case and gathered research in the form of literature reviews DC gathered the investigation results including the figures used in the manuscript DC wrote the final report, ... cut-off sign is used to support the hypothesis of parasympathetic inhibition causing Ogilvie's syndrome [4,7] The diagnosis of Ogilvie's syndrome is widely reported to be troublesome due to the ... histological findings ofthe caecum after right hemicolectomy showed no specific pathology [3] The mechanism ofthe condition is thought to involve loss of tone in the parasympathetic nerves S2 to...
... mgZn/kg based on the uniform distribution of these metals in the sediments ofthe lake The higher concentration in the northwest part ofthe lake is probably due to reverse flow ofthe polluted Pasig ... in the eastern side of Manila Bay The concentration profiles in the sea sediments show the decrease in lead and zinc concentrations in the sediments with the increase in the distance between the ... obvious in the vicinity of these three target cities probably due to dilution by a large amount of suspended solids conveyed by the Pasig River in the case ofthe Philippines Storm water runoff containing...
... rank otherwise identical documents differently just because one ofthe documents is in a larger font The length of a hit list is stored before the hits themselves To save space, the length ofthe ... barrels The main difficulty with parallelization ofthe indexing phase is that the lexicon needs to be shared Instead of sharing the lexicon, we took the approach of writing a log of all the extra ... possibly resulted in the loss ofthe revenue from the airline to the search engine In general, it could be argued from the consumer point of view that the better the search engine is, the fewer advertisements...
... and the lifespan ofthe complex by 10-fold, as judged by koff The physiological chaperone cycle probably consists ofthe rapid binding ofthe N-terminus ofthe partner, regardless of whether ... hypothesize that the protonated state ofthe chaperone initiates this cycle, whereas the deprotonated state occurs upon completion ofthe maturation process ofthe partner The nature ofthe signal ... Ensemble ofthe backbone traces ofthe 20 lowest energy conformers ofthe solution structure of (A) NarG(1–15) and (B) NarG(1–28) second site) The measured enthalpy is the sum of two terms: the reaction...
... which these putative sub-complexes bind to each other during the assembly ofthe bc1 complex is also unknown Furthermore, as in the case ofthe biogenesis of other multi -subunit complexes ofthe ... that the association ofthe Qcr9p subunit with the bc1 complex precedes the binding of ISP In fact, the direct absence of ISP (DISP), or the block of its insertion into the bc1 complex due to the ... subunits incorporated into the bc1 complex On the other hand, the absence of Qcr9p in the DQCR9 ⁄ DQCR10 strain prevented the binding of ISP (Fig 4B) Interestingly, in the absence of Qcr9p, the...
... ofthe structure are not seen on the map, probably owing to disorder of these regions The overall geometry ofthe model according to the procheck program [21] is of good quality, with 95.4% of ... that a-subunits and b-subunits are eluted in the same volumes from the column in the presence of Thr, whereas b-subunits are eluted from the column later than a-subunits in the absence of Thr ... identical to the C-terminal portion ofthe a -subunit, as in CgAK, the dimeric structure revealed in this study represents the structure ofthe regulatory region of an ˚ ab-heterodimer The rmsd is...
... the left side ofthe blots The Western blots are shown in panels A and B and the relative amounts of each ofthe subunits determined by densitometry scanning ofthe stained Western blots ofthe ... mutants [31], the amounts of core and core proteins were significantly influenced by the absence of other subunits ofthe bc1 complex Deletion ofthe genes for subunit 7, subunit or subunit caused ... in the inner mitochondrial membrane or lack of detection ofthe truncated protein by the antibodies In the W303–1B q° strain the amounts ofthe other two catalytic subunits, cytochrome c1 and the...
... in the presence of substrate and a proton motive force [23] On the basis of these studies, it has been proposed that binding of substrate to the TatBC subunits triggers the recruitment ofthe ... rearrangements ofthe TM spans of these subunits It has not been established whether these TM spans simply anchor the amphipathic regions and cytosolic 2272 domains to the membrane, or whether they serve ... expressing the various mutated subunits, and the data for the TatA and TatB mutants (expressed using the pBAD vector) are shown in Fig The expression ofthe wild-type tatABC from the pBAD-ABC...
... the roles of these messages are described in the next sections The following variables are used in the pseudocode: topics is the set of topics that the local node is aware of, msg.source is the ... for topic ½½¼¼ The subscription of node ¼½½½ causes the other two nodes along the route to become forwarders for the topic, and causes them to add the preceding node in the route to their children ... locate the rendez-vous point of a topic If the publisher is aware ofthe rendez-vous point’s IP address then the PUBLISH message can be sent straight to the node If the publisher does not know the...
... activation of Ga16 and Gaq subunits Therefore the lack of change in the rate of degradation cannot be due to lack of receptor-activation ofthe alpha subunits Given that the activation of G proteins ... Accordingly, the levels of Ga16 decay were the same in the presence or absence of carbachol (Fig 4C) in cells expressing the M2R Also the activated mutant of A B C D Fig Functional assay ofthe Ga subunits ... of Gaq and Ga16 have the same behavior as the wild-type forms argues in favor ofthe need of other proteins to stabilize the proteins in the membrane The interaction of Ga subunits with Gbc could...
... years ago Then the estimated replacement rates ofthe different myriapod hemocyanin subunits show a large variance, Fig Stereo view of a model of S coleoptrata hemocyanin subunit A The structure ... sequenced (Fig 3) The cDNA sequences were assigned to distinct subunits (HcA to HcD; Fig 2) by the aid ofthe MALDI-TOF data About 30 peptides that cover a total of at least 40% of each subunit could ... [41,44], although the support values in the present are significantly higher This observation supports the notion ofthe usefulness of hemocyanin sequences for the resolution ofthe arthropod phylogenetic...
... regard, the best combination ofthe two subunits that match the smallest molecular mass is a2b2 Overnight incubation ofthe protein in this detergent led to a decrease ofthe area ofthe first ... did not consider the presence ofthe NrfH subunit; the presence of this integral membrane subunit will decrease the solvent accessibility of hemes and 5, which are located near the putative surface ... as the former simulation as they to the experimental data Thelargesubunit NrfA, contains the high-spin heme, and four low-spin c-hemes with gmax ¼ 3.6, 3.50, 3.2 and 2.96, while the NrfH subunit...
... ability of different b subunits to form dimers composed of two b subunits, of two btes subunits and, ofthe mixture of b/btes subunits These interactions are weaker than interaction of a subunit ... gene including the 161 bp of promoter region joined with the first 12 bp ofthe ORF region was amplified from the DNA ofthe cosmid clone #9 using the same direct primer as for the CK2btes–b-galactosidase ... residues in the b subunit participating in the b–b and b–a intersubunit contacts The analysis of conservation of these residues in the CK2btes sequence has shown that only 19 out of 39 residues...