... Mitsuhashi A, Yamazawa K, Nagai Y, Tanaka N, Mat-sui H & Sekiya S (2004) Correlation between MUC5ACexpression and the prognosis of patients with adenocar-cinoma ofthe uterine cervix. Ann ... part of the figure. The GDPH-cleavage site as well as the epitopes for the mAbs against myc (amyc) and His5(aHis5) are indicated. M-C1,N-terminal cleavage fragment; C2-H, C-terminal cleavage ... by the mAbsagainst M1.Immunoreactivity of mAbs against M1 towards a recombinant MUC5AC C-terminal cysteine-richpartTo test the reactivity ofthe mAbs against M1towards the human MUC5AC C-terminal...
... either the transfer of an entire farm, the leasing or purchase of quota, or the allocation of quota from a national reserve. The transfer of milk quota may take place via a variety of administrative ... 12,312,020,617,019,923,910,46,711,710,114,824,825,08,814,116,817,315,710,88,915,915,128,725,217,613,733,133,50,05,010,015,020,025,030,035,040,0BelgiumBulgariaCzek Rep.DenmarkGermayEstoniaIrelandGreeceSpainFranceItalyCyprusLatviaLithuaniaLuxembourgHungaryMaltaNetherlandsAustriaPolandPortugalRomaniaSloveniaSlovakiaFinlandSwedenUnited ... behind the comparative analysis of these two scenarios is not other than the one given by the data sets to which they are calibrated to DG AGRI outlook for income and agricultural markets (European...
... z, away from the heme normal (z axis), a isthe angle between the projection ofthe tilt ofthe z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location ... [55] had proposed a distal Tyr at position E7 as the source ofthe H-bond to ligand on the basis of a partial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree ... H-bonddonor leads to rapid O2off-rates [19]. Some ofthe mostunusual Hbs characterized to date are those from nematodesand trematodes (mammalian parasites) such as the nematodeAscaris suum (As) [11,12]...
... N- and C-termini [33] and a transmembrane a helix in the central region (Fig. 1 ). On thisbasis, and in view ofthe finding that the C-terminal part of the LH1 a polypeptide plays an important ... except that the NaBr washing step was performed a t 0.6 mgặmL)1totalprotein. The concentration of LH1–RC complex in the isolatedbands was estimated on the basis ofthe total photooxid-izable ... inmutant PufX54*. As in these last two mutants a short lagwas observed (see Table 2), apparently the presence of a stable dimer is not a n ecessary requisite for a fast RC/bc1redox interaction,...
... GCC CGAATT CCT TTC TTT CTT TCT TTC TTT CTT TCT TTCTTT CTT TCT TTC TTT TTT TTT TTC TCG AGCCCC-3Â;antisense:5Â-GGG GCT CGA GAA AAA AAAAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA AGA AAG AAA GAA AGG AAT TCG ... Gutenberg-University, Mainz, Germany;2Axxima Pharmaceuticals AG, Martinsried, Germany The expression ofthe major protein kinase C substrateMARCKS (myristoylated alanine-rich C kinase substrate) is controlled ... proteins target mRNA for rapid degradation remainslargely to be determined. It is not yet clear whether AREsare the actual targets for ribonucleases and/or whethertrans-acting factors lead to an...
... had an estimated Tm of 82 C. The reverse anking primer sequence, 5Â-ATATACATATGAAAGAAAG-3Â, had a calculated Tm of 42 C. The mutagenic primers for each variant are: P1 9A (5Â-AGAGCTGCTAGCAGAGTTG-3Â) ... Each of these variants retains a catalytic activity against yeast RNA comparable with that of parent mBS, indicating that a native conformation is present. A further indication ofthe similarity ... introduction of Ala for Pro19 makes mBS more similarto RNase A, which has a thermal stability higher than that of mBS. However, P1 9A/ L28Q-mBS has the same thermalstability ofthe parent protein,...
... 11507–11512.Supplementary material The following supplementary material is availableonline:Fig. S1. The SARS N protein is phosphorylated bySRPK1 but not by Clk1 and PKA.This material is available as part ofthe ... environmental stress,mRNA metabolism is reprogrammed to adapt tostress-induced damage. Translationally stalled mRNAstogether with a number of translation initiation factorsand RNA-binding proteins are ... vitro translation reactions con-tained 1 lg of indicated recombinant protein as well as32P-labeledluciferase reporter mRNA as tracer. After incubation, the level and the integrity of radioisotope...
... from other Pectinatus strains show a lowmolecular mass band ofthe same mobility as in the strain E-82164, and a ladder-like pattern, characteristic of the presence ofthe O-chain. No bands analogous ... AcknowledgementsThis work was supported by the Canadian Bacterial Diseases Network.We thank Donald Krajcarsky (NRC Canada) for the ESI MS analysis. The spectra at 800 MHz were obtained on the ... 3.Deamination ofthe de-O,N-acylated LPS and preparation of oligosaccharides 4 and 5 The mixture of oligosaccharides obtained after alkalinedeacylation ofthe LPS (200 mg) was treated with 300 mgNaNO2in...
... potentials.Steady-state enzyme activities The catalytic activity ofthe NR1-FAD/NADPH domainwas examined and compared with the CPR FAD/NADPHdomain. The CPR FAD/NADPH domain retains trans-hydrogenase ... cofactor, and they displayed UV-visible absorp-tion spectra characteristic of flavin containing enzymes(Fig. 2B). The fully oxidized FMN domain had absorbancemaxima at 376 and 454 nm, and the ... 2A) . In the case ofthe NR1-FAD/NADPH domain, a second 2Â,5Â-ADP-Sepharose afnity step was also incor-porated in the purification scheme, taking advantage of itsnucleotide binding capacity of...
... O-polysaccharide of Pr. alcalifaciens O23 [23], and an amide ofthe same aminoacid withD-galacturonic acid (structure 2) in the O-polysac-charides of P. mirabilis O13 [24]. An amide of D-galacturonicacid ... gel-per-meation chromatography on Sephadex G-50. Analysis of the polysaccharide using an amino-acid analyzer revealed the presence of GlcN and GalN in the ratio 2 : 1 as well asanother amino component. ... L water) to give a high-molecular-mass O-polysaccharide in a yield of 20% of the LPS weight.Rabbit antisera and serological assaysPolyclonal O-antisera were obtained by immunization of rabbits...
... REPORT AVAILABLE AT THE TIME OFTHE AUDITSubject Argentina Israel IndiaNew ZealandTunisia1Costa RicaMonitoring and supervisory activities carried out by the competent authority in the third ... authorisation in the specific module of OFIS. The module gives the Commission and the Member States access to up-to-date, standardised information on all import author-isations granted across ... composed ofa table with the number of visits carried out by the various private inspection bodies, the number of samples taken for analysis and the number of irregularities found and penalties applied....
... of aminoacids) than each ofthe muscle AChR subunits (22%on average) necessitates the solution ofthe structure of the mammalian AChR molecule. A prerequisite forthis isthe availability of ... e1219variants (e1220 with additional Pro), the forward pri-mer 5Â-GGTGTAGAATTCAAGAACGAGGAACTGCG-3Âwas combined with (a) 5Â-ATAGTTTAGCGGCCGCTTACTTCCGGCGGATGATGAGCGAG-3Â for e1219,(b) 5Â-ATAGTTTAGCGGCCGCTTAGTGATGGTGATGGTGATGCTTCCGGCGGATGATGAGCGAG-3Â ... 5Â-ATAGTTTAGCGGCCGCTCAATGGTGATGGTGATGGTGCTTGCGGCGGATGATGAG-3Â. For the c1218 variants (some with an additional C-terminal Progiving c1219), the forward primer 5Â-GGTGTAGAATTCCGGAACCAGGAGGAG...
... leads us to assert that: 1. A number of potentially relevant analytical approaches have thus far been employed primarily at the national level; though these approaches often appear to have the ... information needs ofthe plural America that we are becoming. These changes pose immediate analytic challenges for policy makers and regulators. Available data and research indicate that: 1) There ... marketplace dynamics. They include issues of access to capital, as well as the dynamics ofthe advertising marketplace, which frequently appear to demonstrate the under-valuing of minority audiences...
... the provinceinexchangefor the support of the Polesin the Austrianparliament. with a Polisharmy organizedinAmerica, of un-Amer-icanized Polishimmigrants,begananoffensiveagainst the Ukrainians,attackingthem from the rear. ... upon the apparentauthenticity of an Asso-ciatedPressdispatchdated atParis,November21,1919,inwhich it is stated that the SupremeCouncil hasagreedtograntPoland a mandateoverEasternGalicia. The dispatchstates:"By the terms ... scienceinLembergand a strongdemocraticpress, the Ukrainianshavedemonstrated the abilitytogovernthemselves.Polishstudents of Galiciahavetestifiedthat the level of civicandcul-turaldevelopment of the GalicianUkrainianfarmer is higherthan that of the Polishfarmer of WesternGalicia.("Galicia,"byF.Bujak,Cracow,1908.)11...
... Immunohistochemicalcharacterization of multinucleated giant cells in the brain of a Japanese AIDS patient. Acta Pathol Jpn 1991, 41:212-216.65. Finkel TH, Banda NK: Indirect mechanisms of HIV pathogene-sis: ... thatVpu could also act at these stages to accomplish the samegoals, given that it also causes membrane leakage, it is more difficult to envision how it could accomplish the task as Vpu has ... vir-ion.Since the LLP domains are also present in the context of the virion, it is possible that they would have an effect atthis stage ofthe HIV replication cycle. There is at least onereport of an...