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identification of altered rhd and rhce alleles a comparison of manual and automated molecular methods

Báo cáo sinh học:

Báo cáo sinh học: " Identification of a major gene in F and F data when alleles 1 2 assumed fixed in the parental lines" pps

Báo cáo khoa học

... locus A with alleles A and A where A has frequencies fp and in the paternal and maternal line Genotype l m l frequencies, values and numeration are given for F individuals as: A base-population of ... backcross data , l assuming fixation of alleles in parental lines, genotypes of parents are assumed known and all equal and this analysis leads to the fitting of a mixture distribution without accounting ... for family structure Fitting of mixture distributions has been proposed when pure line and backcross data as well as F and F data are available, and when parental lines are homozygous i for all...
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Báo cáo khoa học: Mammalian transglutaminases Identification of substrates as a key to physiological function and physiopathological relevance pot

Báo cáo khoa học: Mammalian transglutaminases Identification of substrates as a key to physiological function and physiopathological relevance pot

Báo cáo khoa học

... b-sandwich, a core (which contains a transamidation site and a Ca2+-binding site, and has a helices and b sheets in equal amounts), and two C-terminal b-barrel domains It has been suggested that glutamyl ... extrusion of TG4 from the coagulating gland [129] FXIII Coagulation FXIII is a plasma TG, and circulates in blood as a heterotetramer consisting of two catalytic A (XIIIA) and two noncatalytic B ... the manuscript, Drs Francesco Facchiano and Angelo Facchiano for their help in consulting the TRANSIT database, and Dr Eleonora Candi for her contribution in the preparation of Table We are grateful...
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báo cáo hóa học:

báo cáo hóa học:" Identification of a public CDR3 motif and a biased utilization of T-cell receptor V beta and J beta chains in HLA-A2/Melan-A-specific T-cell clonotypes of melanoma patients" potx

Hóa học - Dầu khí

... CTTGGC GCCAGCAG ATACCA GGGAC TAGGA 39 GCCTGGAGTGT C C CAGGG CTAGG NA XXXXXXAGT CAT CAGGG ATTGGG 16 GCCAGCA CCCT GACAGGG CTTGGA 6E4 GCCAGCAGTTT TCT 40 GCCAGCAGTTTA B/22 GCCAGCAGT C A .ACAGGG ... .CAGCCCCAGXX X .CAGCCCCAGCA T TTCACCCCT CCAC TCAGCCCCAGXX X TCAGCCCCAGXX X TCAGCCCCAGCA T .CAGCCCCAGCA T .CAGCCCCAGCA T .CAGCCCCAGCA T TCAGCCCCAGCA T CTATGGCTACXXX TCAGCCCCAGCA T not available tions 105, ... GCCAGCAG CCA .ACAGGGG CTCGG B/9 GCCAGCAGTTT TCA GGGAC TCGG aNucleotides 5' J region C GGG TTGGG CAGCCCCAGCA T .TATGGCTACACC .CAGCCCCAGCA T .CAGCCCCAGCA T .CAGCCCCAGXX X .CAGCCCCAGXX X .CAGCCCCAGCA...
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Báo cáo hóa học:

Báo cáo hóa học: " Identification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full genome" pdf

Hóa học - Dầu khí

... Microarray-based detection and genotyping of viral pathogens Proc Natl Acad Sci U S A 2002, 99:15687-15692 Allander T, Andreasson K, Gupta S, Bjerkner A, Bogdanovic G, Persson MA, Dalianis T, Ramqvist ... Isolation and identification of a novel human parechovirus J Gen Virol 2004, 85:391-398 Abed Y, Boivin G: Molecular characterization of a Canadian human parechovirus (HPeV)-3 isolate and its relationship ... linkers for the HinP1I-site (GACGATGAGTCCTGAT and Phosphate-CGATCAGGACTCAT, 1:1) and the CviII-site (CTCGTAGACTGCGTACG and Phosphate-ATCGTACGCAGTC, 1:1) were ligated to the complete phenol-purified...
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báo cáo hóa học:

báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx

Hóa học - Dầu khí

... primer pairs from Integrated DNA Technologies: Muc16 5'-TGCCACCTACCAGTTGAAAG-3' and 5'-GTACCGCCAAGCAGATGAG-3'; GAPDH 5'-TGCTGAGTATGTCGTGGAGTCTA-3' and 5'AGTGGGAGTTGCTGTTGAAGTCG-3' The amplified ... murine monoclonal antibodies that were initially generated against human MUC16 List of abbreviations EOC: epithelial ovarian cancer; ConA: Concanavalin A; αMe-Man: α-methylmannopyranoside Competing ... western blot ananlysis and was assisted in these experiments by JAB and JAAG MM and CR helped in designing appropriate Muc16 primers JC assisted in obtaining and maintaining murine ovarian tumor...
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Báo cáo khoa học:

Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt

Báo cáo khoa học

... isolation and identification of a potential viral pathogen was pursued Canine coronavirus was first isolated from a case of canine enteritis during an epizootic in Germany in 1971 Later, Woods and ... Hayek LA, Dubovi E, Zhang Z, Yan Q, Guo W, Wildt DE Serosurvey of ex situ giant pandas (Ailuropoda melanoleuca) and red pandas (Ailurus fulgens) in China with implications for species conservation ... Harrison GA, Monckton RP, McOrist S, Lehrbach PR, Deane EM Identification of canine coronavirus strains from feces by S gene nested PCR and molecular characterization of a new Australian isolate J...
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báo cáo khoa học:

báo cáo khoa học: " Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library" pot

Báo cáo khoa học

... potential for use in early diagnosis and targeted therapy of RCC Materials Renal carcinoma line A4 98 and a normal renal cell line HK-2 were obtained from Medical Academy of China (Beijing, PR China) ... Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library Xiangan Tu1*§, Jintao Zhuang1*, Wenwei Wang1, Liang Zhao1, Liangyun Zhao1, Jiquan Zhao1, Chunhua ... Moch H Diagnostic and prognostic molecular markers for renal cell carcinoma: a critical appraisal of the current state of research and clinical applicability Eur Urol, 2009, 55(4): 851–863 Pande...
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báo cáo khoa học:

báo cáo khoa học: " Identification of drought-response genes and a study of their expression during sucrose accumulation and water deficit in sugarcane culms" potx

Báo cáo khoa học

... absorption at 254 nm External standards were made using the Waters physiological amino acid standard with addition of Asn, Gln, GABA and Trp (Sigma) This standard was diluted to make a standard curve ... Imai A, Akiyama T, Kato T, Sato S, Tabata S, Yamamoto KT, Takahashi T: Spermine is not essential for survival of Arabidopsis FEBS Letters 2004, 556:148-152 39 Imai A, Matsuyama T, Hanzawa Y, Akiyama ... Nielsen, Jai Perroux, Mark Jackson, Michael Hewitt, Chris Grof and Anne Rae for harvesting the plant material, Donna Glassop for harvesting and collection of sugar analysis data by HPLC, and to...
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báo cáo khoa học:

báo cáo khoa học: "Identification of improved IL28B SNPs and haplotypes for prediction of drug response in treatment of hepatitis C using massively parallel sequencing in a cross-sectional European cohor" pot

Báo cáo khoa học

... Mita E, Tanaka E, Mochida S, Murawaki Y, Honda M, Sakai A, Hiasa Y, Nishiguchi S, Koike A, Sakaida I, Imamura M, Ito K, Yano K, Masaki N, Sugauchi F, Izumi N, Tokunaga K, Mizokami M, Tanaka Y, ... 45:903-910 Page 13 of 13 25 Honda M, Sakai A, Yamashita T, Nakamoto Y, Mizukoshi E, Sakai Y, Yamashita T, Nakamura M, Shirasaki T, Horimoto K, Tanaka Y, Tokunaga K, Mizokami M, Kaneko S: Hepatic ISG ... IL28B Variant calling and association testing CRISP detected 1221 SNVs and 118 indels (1339 variants in total) Validation with original GWAS SNP data and HapMap data CRISP called all 15 of the...
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Báo cáo y học:

Báo cáo y học: "Array comparative genomic hybridisation-based identification of two imbalances of chromosome 1p in a 9-year-old girl with a monosomy 1p36 related phenotype and a family history of learning difficulties: a case report" pps

Báo cáo khoa học

... dysmorphism, clinodactyly of the fifth finger and mild learning disability The maternal grandmother was also assessed and was phenotypically normal The proband's father was not available for assessment, ... clinodactyly and short toes She had required grommets for treatment of glue ear and had hypermetropia An 11-week scan detected nuchal oedema and ventriculomegaly was detected on a later antenatal ... paternal sample was unavailable but FISH analysis of the mother, grandmother and siblings of the proband revealed a normal diploid compliment in each case for all of the investigated clones At present,...
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Báo cáo y học:

Báo cáo y học: " Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus" doc

Báo cáo khoa học

... TGCAGGGATCCATGCAGTTAGATGGTGACAAT CTTTGGTCGACTTATTCCCCCGGATAATAGAT 1540-1575 36 F7 TGCAGGGATCCATGTTAGATGGTGACAATATC CTTTGGTCGACTTATTCCCCCGGATAATAGAT 1543-1575 33 F8 TGCAGGGATCCATGGATGGTGACAATATCTAT ... CTTTGGTCGACTTATTCCCCCGGATAATAGAT 1552-1575 24 F11 TGCAGGGATCCATGAATATCTATTATCCGGGG CTTTGGTCGACTTATTCCCCCGGATAATAGAT 1555-1575 21 F12 F13 TGCAGGGATCCATGATCTATTATCCGGGGGAA CTTTGGTCGACTTATTCCCCCGGATAATAGAT GATCCATGATCTATTATCCGGGGTAAG ... GTCGACTTACAGCTGCCCTCCCTGGAC 1042-1347 306 F2 GGATCCATGTATGGACAGCCTGTTTAT GTCGACTTAAGCTAATGGTCCAGTAGA 1294-1731 438 F3 GGATCCATGCCTACTGGACAAGGTAAC GTCGACTCAACATCTATTACACATCA 1681-2124 444 F2-1 GGATCCATGTATGGACAGCCTGTTTAT...
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báo cáo khoa học:

báo cáo khoa học: " Identification of a Rice stripe necrosis virus resistance locus and yield component QTLs using Oryza sativa × O. glaberrima introgression lines" docx

Báo cáo khoa học

... sativa ssp tropical japonica) is a commercial rice variety developed by EMBRAPA-CNPAF (Goiania, Brazil) and has been cultivated since 1992 in Brazil and other places in Latin America and the Caribbean ... selected as a mapping population for agronomic evaluation and molecular characterization [Additional file 1: Figure S1] Phenotypic evaluation Methods Plant materials The recurrent parent Caiapó (O sativa ... Ward E, Castaño M, Arroyave J, Lozano I, Adams M: Emergence and partial characterization of Rice stripe necrosis virus and its fungus vector in South America European Journal of Plant Pathology...
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báo cáo khoa học:

báo cáo khoa học: " Analysis of a c0t-1 library enables the targeted identification of minisatellite and satellite families in Beta vulgaris" ppt

Báo cáo khoa học

... TGTGACTTGTAACATTGCGCGGGTGCTTGGCACCATTTGCGTTACCTCAAA AAGCCTTTGAACACCCCAATTATTCATTTCTCGCGAAATCCAAAATTGCCT CGAAATGAACGTAAAGGCATCCACATATTTGTTCCAAGCCACATGACTCCT TTACATTGACCTCCTATGTCCCTAGGAGGCATCCCGTGCCATTTGGAGCTC GGGCAACGGGAAAGTCCGAAAGCGTGTATAATCTTCAATTTTAGTTGTTTT ... ACTGAAAAAAAATGAAGACTA 32 90 - 100 ED019743 BvMSat08 GAAAAAATAAGTTCAGATCAGATCAGATCA 32 48 77 - 100 DX107266 GGGTCGGAATAAATCGGCTTTCGAAATGACTT BvMSat09 32-39 24 46 - 100 FN424406 AGAAGTATACAAGAACATTAATCAAAATATATAAACAAA ... DX983375 CCTCTAAATGTAAGTGGCTTTAGCAGCACTATAAGTTCTGTGCCTAAAAAA FokI-satellite 130 60 81 - 100 DX979624 GGGACTTAGGAGAGTGACCCAACCAAGGAGGGAGACCTCCTTGGGCTGAGT GGTGGCATTACGGGCAACCAACAATTAGCGACAGGCATATGGTTG...
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báo cáo khoa học:

báo cáo khoa học: " Identification of genes associated with growth cessation and bud dormancy entrance using a dormancy-incapable tree mutant" doc

Báo cáo khoa học

... 5’-ACCTCCACTGTTGCACCTCAC-3’ 5’-CAGAGGGCAAGCAACTACCAC-3’ R 5’-CCAGAGAAATTATGGAAGCCCCA-3’ Dormancy associated MADS-box gene (PpDAM6) GE653238 F 5’-CCAACAACCAGTTAAGGCAGAAGA-3’ R 5’-GGAAGCCCCAGTTTGAGAGA-3’ ... GE653334 R F 5’-TTCTACTGCCACAGCCAGCA-3’ 5’-ATGGCAAACCACCAAGCACTCA-3’ R 5’-GTAGAGGAGCCTTGATTGGAGGAG-3’ Unknown3 GE653309 F 5’-AAGTTGTCCATCCCAACACATTCG-3’ R 5’-GCGAGGACATCTCTGGCAATAAGA-3’ Unknown4 GE653307 ... 5’-GGAAGCCCCAGTTTGAGAGA-3’ Epicotyl-specific tissue protein GE653203 F 5’-CACCAAAAGAGAAAGCCGACTGC-3’ R 5’-TCAACCTCAACGTCAACCTCAAC-3’ RD22 (dehydration-responsive) precursor GE653312 F 5’-GAACCCACACAAGATTATCAGCAGG-3’...
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Báo cáo y học:

Báo cáo y học: "Identification of target antigens of antiendothelial cell and anti-vascular smooth muscle cell antibodies in patients with giant cell arteritis: a proteomic approach" potx

Báo cáo khoa học

... extracts: mitochondrial fumarate hydratase, lamin A/ C and vinculin IgG reactivity against vinculin and lamin A/ C in sera from GCA patients and the HC pool are depicted in Figure and Additional ... [10,35] AECAs have been associated with disease activity in patients with vasculitis, particularly in those with anti-ANCA-associated vasculitis, Takayasus arteritis or GCA [15], although these data ... two-thirds of the patients had active disease as assessed by a Birmingham Vasculitis Activity Score (BVAS) >3 in the absence of treatment, and one-third of the patients had inactive disease as assessed...
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Báo cáo y học:

Báo cáo y học: "Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit: a new "syncytin" in a third order of mammals" ppt

Báo cáo khoa học

... representation of a rabbit placenta (right) and haematoxylin and eosin staining of a day 12 placenta section (left) with the main layers of the placenta indicated (B) Higher magnification of the areas framed ... using an ABI PRISM 7000 sequence detection system Primer sequences were as follows: 5'-GCTGTTTTTATGCTAACAAGTCC and 5'-GATAAAGGTCATCAGC CT ATTGA for Env-Ory1 and 5'-CCTCTAAATGTCATCTTCACCAG and 5'-CTATTGGGACAGCAGTTCTAGTC ... Ensembl databases Conclusion Here we have shown that within a placental mammal that has developed a haemochorial placenta with a syncytiotrophoblast layer at the maternal-fetal interface, such as the...
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Báo cáo y học:

Báo cáo y học: " Identification of a novel resistance (E40F) and compensatory (K43E) substitution in HIV-1 reverse transcriptase" doc

Báo cáo khoa học

... (5' GAA ATT TGT ACA TAG CTG GAA AAG G-3', nucleotides 2655–2679), 40F-RT2 5' GAA ATT TGT ACA TTG CTG GAA AAG G-3', nucleotides 2655–2679) and 40F-RT3new 5' GAA ATT TGT ACA TTT CTG GAA AAG GA-3', ... algorithms that are used to manage patients failing nucleoside therapies Methods Analysis of E40F and K43E prevalence and associations A dataset of 161,974 deidentified HIV-1 subtype B clinical samples ... 2664–2688), 43E-RT (5' ACA GAG CTG GAA GAG GAA GGG AAA A- 3', nucleotides 2664–2688) and 43E- http://www.retrovirology.com/content/5/1/20 RTA (5' ACA TTT CTG GAA GAG GAA GGG AA-3', nucleotides 2664–2686)...
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Báo cáo y học:

Báo cáo y học: " Identification of structural aberrations in cancer by SNP array analysis Stefan Heinrichs and A Thomas Loo" pot

Báo cáo khoa học

... 7, Article 219 Heinrichs and Look Advantages of SNP array analysis A SNP is defined as a DNA sequence variation at one specific position in the genome that occurs in at least 1% of the human ... clustering of SNP-array-based loss -of- heterozygosity data Bioinformatics 2004, 20:1233-1240 26 dChip Software: Analysis and Visualization of Gene Expression and SNP Microarrays [http://biosun1.harvard.edu/complab/ ... conventional cytogenetic analysis The identification of PAX5 and EBF1 as new mutational targets in early B-lineage leukemogenesis shows the value of SNP array studies for selecting genes for detailed analysis...
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Báo cáo y học:

Báo cáo y học: "Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors" pot

Báo cáo khoa học

... Biosystems) Data Our data are freely available at the proteome database of the department of proteomics and signal transduction of the Max-Planck-Institut for Biochemistry [45] Additional data files ... following additional data are available with the online version of this paper Additional data file lists all peptides and protein hits obtained in both LTQ-FT and LTQ-Orbitrap data Click here data Orbitrap ... intense peaks for obtaining MS2 data refereed research Samples of closed-eye tear were collected from one of us (GAS) using a μl calibrated glass microcapillary tube (Blauband intraMARK, Brand GMBH,...
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IDENTIFICATION OF A MINIMAL CIS-ELEMENT AND COGNATE TRANS-FACTORS REQUIRED FOR THE REGULATION OF RAC2 GENE EXPRESSION DURING K562 CELL DIFFERENTIATION

IDENTIFICATION OF A MINIMAL CIS-ELEMENT AND COGNATE TRANS-FACTORS REQUIRED FOR THE REGULATION OF RAC2 GENE EXPRESSION DURING K562 CELL DIFFERENTIATION

Y khoa - Dược

... 27, 36 and 79 in H3 and on position 20 in H4 (Nakayama, Rice et al 2001) Methylation of histone H3K4 and H3K36 are associated with activation of transcriptional activity, and histone H3K9 and H3K27 ... Aurora -A and Aurora-B, have been identified as the histone H3 kinase (Crosio, Fimia et al 2002) A balance of protein phosphatase and kinase activities is needed to maintain steady state levels of ... enzymatically inactive due to amino acid change at a potentially catalytic site (Pinarbasi, Elliott et al 1996) Human Dnmt2 does not catalyze methylation of DNA, but instead methylates a small RNA-...
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