harvesting bladder and iliac lymph nodes for immune cells recruitment analysis

Mycobacterium bovis, BCG modulation of gene expression in bladder cancer

... cells for RNA isolation and SuperArray analysis …… 44 2.2.4.3 Harvesting bladder and iliac lymph nodes for Immune cells recruitment analysis 44 2.2.4.4 RNA isolation……………………………………………………………………… ... Intensity analysis of RT-PCR bands from the cDNA samples of mice bladders 78 Table 3.4 Percentage of immune cell recruited to the bladder and iliac lymph nodes 81 Table 4.1 Integrins α5 and β1 expression ... specimens…………………… 75 3.2.2 RT-PCR analysis of BCG induced gene up-regulation in the mouse bladder …… 76 3.3 Increased lymphocytes were observed in the bladder and iliac lymph nodes (ILN) after BCG instillation………………………………………………………………...

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PURIFICATION OF ORGANIC CHEMICALS pot

Danh mục: Tự động hóa

... agent, and CaC12 forms an addition compound Drierite (anhydrous CaS04) offers the minimum acid and base catalysis of aldol formation and is the recommended drying agent for this solvent [Coetzee and ... Dippy and Evans [JOC 15 451 19501 let the anhydride (500g) stand over P2O5 (50g) for 3h, then decanted it and stood it with ignited K2CO3 for a further 3h The supernatant liquid was distd and the ... trimethylacetic acid and C9 and C13 acids Dissolve 15g of acid in CCl4 (3OOml) and shake with lOml of 15N aqueous NH3 and the ammonium salt separates and is collected Acid impurities form soluble ammonium...

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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

Danh mục: Báo cáo khoa học

... or the difference between K2 and K1, and the reduction in band width Within the eight tested solvents systems, the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ... (2H, m, Ha-3 and Ha-5), 1.47 (2H, m, Ha-2 and Ha-6 ), 1.61 (2H, m, Hb-2 and Hb-6 ) and 2.00 (2H, m, Hb-3 and Hb-5 ) The 13C NMR spectrum of compound displayed signals at d: 22.8 (C-3 and C-5), 27.5 ... not be separated from each other by CCC Since K2 and K4 were close to K3 and K5, respectively, curcumol (2) and germacrone (3), and curzerene (4) and 1,8-cineole (5) were hardly separated from...

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PURIFICATION OF LABORATORY CHEMICALS pot

Danh mục: Tự động hóa

... acid Reactive halides and anhydrides Salts and organic esters of some inorganic acids Sol vents 5 5 6 6 METHODS OF PURIFICATION SOLVENT EXTRACTION OF REAGENTS AND SOLVENTS AND DISTRIBUTION DISTILLATION ... adsorbents and solvents Preparation and standardisation of alumina Preparation of other adsorbents Silica Diatomaceous earth Charcoal Cellulose Supelco products Flash Chromatography (FC and HPFC) ... glassware and plasticware 3 SAFETY PRECAUTIONS ASSOCIATED WITH THE PURIFICATION OF LABORATORY CHEMICALS SOME HAZARDS OF CHEMICAL MANIPULATION IN PURIFICATION AND RECOVERY OF RESIDUES Perchlorates and...

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purification of carbon nanotubes

Danh mục: Vật lý

... reﬂuxed in M nitric acid for 12, 24 and 48 h, in M nitric acid for and 12 h, and in concentrated nitric acid for and 12 h The weight percent of each component calculated from TGA and NIR spectra is ... oxidants for gas phase oxidation include air [40–46], a mixture of Cl2, H2O, and HCl [47], a mixture of Ar, O2, and H2O [48–50], a mixture of O2, SF6 and C2H2F4 [51], H2S and O2 [52], and steam ... Electrochemical oxidation for tip opening and puriﬁcation of MWCNT arrays was performed in an aqueous solution of 57% H2SO4 at room temperature SEM and TEM images before and after puriﬁcation (Fig...

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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot

Danh mục: Báo cáo khoa học

... 20 mm Glu and 20 mm glyoxylate for GGT, 20 mm Glu and 30 mm pyruvate for GPT, 40 mm Ala and mm glyoxylate for AGT, and 10 mm phosphoserine and 10 mm 2-OG for PSOT For the AT1 assay, the pH in the ... was added For GOT, GGT, GPT, AGT and PSOT assays, substrate concentrations were modiﬁed as follows: 100 mm Glu and 10 mm oxaloacetate or 1882 10 mm Asp and 10 mm 2-OG for GOT, 20 mm Glu and 20 mm ... AT2 and AT3 gave single bands of 44, 42 and 45 kDa on SDS ⁄ PAGE, respectively (Fig 2) The N-terminal amino acid sequences of AT1, AT2 and AT3 were determined to be MNLSKRVSHIKPAPT, MYQERLFTPG and...

10
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armarego - purification of laboratory chemicals 5e

Danh mục: Hóa học - Dầu khí

... scales e.g the H0 (for acids) and H (for bases) have been used [see Katritzky and Waring J Chem Soc 1540 7962] These values will be less than (and negative) for acids and >11 for bases They are ... nitric and sulfuric) and formic acid Other examples are acetic acid-pyridine, acetone-chloroform, aniline-phenol, and chloroform-methyl acetate The following azeotropes are important commercially for ... Its main use is for preliminary drying of alkyl and aryl halides, most esters, saturated and aromatic hydrocarbons and ethers Unsuitable for drying alcohols and amines (which form addition compounds),...

655
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot

Danh mục: Hóa học - Dầu khí

... 10% FCS for 24 h The cells were washed and RPMI containing 2% FCS was added and incubation continued At time points h, 1, 3, and days post infection (dpi), cells and media were harvested for DNA ... HHV-6A- and mock-infected JJHAN cells at dpi Note the CPE, i.e enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected ... purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped to draft the manuscript All authors read and...

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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx

Danh mục: Hóa học - Dầu khí

... 10% FCS for 24 h The cells were washed and RPMI containing 2% FCS was added and incubation continued At time points h, 1, 3, and days post infection (dpi), cells and media were harvested for DNA ... HHV-6A- and mock-infected JJHAN cells at dpi Note the CPE, i.e enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected ... purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped to draft the manuscript All authors read and...

11
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc

Danh mục: Hóa học - Dầu khí

... cycling conditions: 98°C for one 15 second (sec) cycle and then 35 repeated cycles of 98°C for sec, 59°C for 10 sec, and 72°C for 15 sec, followed by a final extension at 72°C for minutes (min) LASV ... cycling conditions: 98°C for one 30 sec cycle and then 35 repeated cycles of 98°C for 10 sec, 59°C for 15 sec, and 72°C for 30 sec, followed by a final extension at 72°C for Table outlines each ... protein was isolated For MBP-GP2, vector pMAL-c2x and E coli Rosetta gami cells were also best suited for expression, with optimal induction performed using 0.15 mM IPTG at 30°C for h In this manner,...

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Báo cáo hóa học: " Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA complexes" doc

Danh mục: Hóa học - Dầu khí

... isolated and subjected to radiolabelled primer extension analysis to detect segment 5, and 2-specific RNA B Cells were fixed at hpi and analysed by confocal microscopy after staining for NP (green) and ... [70,44] For metabolic labelling of proteins, 293T cells were washed twice with methioninefree medium for and then incubated for h with 60 nCi/μl of 35S-methionine For immunoprecipitation, × 105 cells ... 0.1 mM EDTA and centrifuged at 100,000 gav for 13 h [73] Fractions were then collected and analysed for their protein and RNA content by western blot and primer extension respectively For this,...

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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx

Danh mục: Hóa học - Dầu khí

... Detection temperature for (+)-1, (-)-1, 13, 14 and 15 was 60°C for followed by a gradient of 10°C/min to 180°C maintained for Detection temperature for 16 was 120°C For 17, 240°C for followed by a ... expression analysis Gene expression analysis was performed with crude cell extract Samples standardized to cell amount were taken during cultivation Cells were harvested by centrifugation and resuspended ... gradient of 2°C/min to 270°C was used and maintained for 6-8 were analyzed at 60°C and 10 were detected isothermal at 160°C Detection temperature for 11 was 90°C and for 12 100°C Specific activity is...

8
548
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purification of some heavy metals from paper making

Danh mục: Môi trường

... heavy metals by wetland reed in the different growth periods and parts Furthermore, it provides the theoretic basis and the technical support and analyzes the mechanism of wetland reed purifying ... jointing stage, removal rate to Cu 37% and 29% at heading and maturity stage,exhibition leaf stage and sprout stage is lower 19% and 13% respectively Through analysis of variance of reed removal ... metals in wetland, but differences of root in structure may also affect reaction process For example, thick roots and rootlets, deep roots and shallow roots may have different capacities for the absorption...

6
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báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps

Danh mục: Báo cáo khoa học

... continued for hours and then processed for protein extraction During the expression processes, a sample of 250 μl was taken at the end of each hour for SDS-PAGE analysis Cell lysis and protein ... pH 8.0 for SiMAC-Nickel bead and 50 mM, 100 mM, 250 mM and 500 mM imidazole for SiMAG/N-NTA/Nickel and SiMAG/CS-NTA/Nickel beads) Briefly, 200 μl of elution buffer was added to the beads and mixed ... 20 and 40 μl of SiMAC-Nickel bead (100 mg/ml) corresponding to the final concentration of 1, 2, and mg/ml, respectively, and 5, 10, 20, 40 and 80 μl of SDS- PAGE and Western blotting SDS-PAGE analysis...

11
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Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx

Danh mục: Báo cáo khoa học

... produce multivalent vaccine candidates The current diagnostic tests and vaccines for PPV are well established and considered to be adequate for most practical purposes; therefore the authors would ... current study was to find an improved procedure for expressing PPV VP2 in vitro and to purify the fusion protein Materials and methods 2.1 Cells, virus, and reagents Strain 20-06 of PPV was isolated ... White colonies contained the recombinant bacmid, and therefore were selected for isolation of recombinant bacmid DNA Before DNA was isolated, candidate colonies were streaked to ensure they were...

6
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt

Danh mục: Báo cáo khoa học

... Nasal-associated lymphoid tissue contains specialized M cells, which can selectively sample and internalize lectins in the nasal mucosa to be presented to T cells by dendritic cells, macrophages and B cells ... lectin activity and therefore, it is likely that after i.n administration, binding to M cells by RTB increased the uptake and transport of p24 to the nasal lymphoid tissue On the other hand, we found ... peritoneal cavity resulted in its transport to lymph nodes where the fusion could be internalized by antigen presenting cells and presented to T and B cells There are some instances in which RTB...

11
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PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1

Danh mục: Y khoa - Dược

... cytotoxic T cells as well as memory T cells The helper T cells remain in the lymph tissue where they secrete cytokines and chemokines to manage the activity of B cells, cytotoxic T cells, and macrophages ... between the innate and adaptive response These cells when activated become antigen presenting cells that transport antigens to the lymph tissues that house the lymphocytes (B and T cells) (Austyn ... control groups of cells Two groups of endothelial cells were incubated with 50 ng of rHu IL-1 and rHu TNFα respectively for 20 The cells underwent methanol fixation and were then stained for the p65...

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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene

Danh mục: Tiến sĩ

... Shih, for his remarkable mentorship He treated me more as a family than a student I respect him for his honesty, his enthusiasm in science and work, his always welcoming attitude for discussion, and ... DiMario, and Anne Grove for guiding me with their expertise, always welcoming attitude and providing access to their lab equipment and reagents I would like to thank Dr Raymond Schneider and Dr ... and Dr Barbara Smith for providing strawberry plants, fungal cultures and their expertise in their area of research I would also like to thank my lab colleagues, Anwar A Khan and Yanlin Shi for...

119
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purification of phytase from aspergillus fumigatus

Danh mục: Tổng hợp

... and 90% saturation (Appendix 1) The solution was stirred and kept at 4°C for – hours, then centrifuged at 13000 rpm for 20 minutes The pellet of each ammonium sulfate fraction was collected and ... Protein content and specific activity of precipitated fractions were determined by Bradford (1976) and Heinonen and Lahti, (1981) methods respectively SDS-PAGE was used to check for phytase purity ... 2.2.3.1, and centrifuged at 7000 rpm for 20 minutes Dissolving the pellet in the buffer Tris-HCl 0.02M pH 7.6 + AS 30% saturation, and dialyzed against the same buffer in the fridge for 24 hours...

20
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THE CONTRIBUTION OF CLAMS ON TIDAL FLAT PURIFICATION CAPACITY

Danh mục: Môi trường

... samples for quinone were stored at –20oC until using for the analysis - 84 - Journal of Water and Environment Technology, Vol.2, No.2, 2004 Laboratory experiment: experimental set up and procedure ... of chloroform-methanol and then re-extracted into hexane Menaquinones and ubiquinones contained in the crude extract were separated and purified using Sep-Pak® Plus Silica The type and concentration ... and immersion on the porewater nutrient dynamics of an intertidal sandflat in Tokyo Bay Estuarine Coastal and Shelf Science, Vol 57, 929-940 Lester, J.N and Birkett, J.W (1999) Microbiology and...

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