resident and infiltrating immune cells

... hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone Mauro Maccarrone 1,2 , Valeria Gasperi 3 , Filomena Fezza 1 , Alessandro Finazzi-Agro ` 3 and Antonello Rossi 3 1 Department ... seems noteworthy that leptin [42,43] and progesterone [44,45] play roles in survival, death and proliferation of immune and neuronal cells; processes in which AEA and the endocannabinoid system are ... of AMT, NAT and PLD in the same cells (Figs 1A,B,D and data not shown). It should be recalled that also CHP100 cells are d evoid o f c annabinoid receptors [18]. Both U937 and CHP100 cells have...

Ngày tải lên: 30/03/2014, 15:20

... of cells after the diet to the percentage of cells before the diet) and the percentage of body fat lost by each subject during the study. A correlation also exists between CD34+ cells and ... weight adults, and the colony-forming capac- ity of these cells is blunted [17, 18]. Alterations in en- dothelial cells and EPC function associated with obe- sity precede atherosclerosis and thrombosis ... blood–derived cells from which endothelial cells in culture were developed were described as cells expressing CD34 antigen. It has been hypothe- sized that endothelial progenitor cells and hemato- poietic...

Ngày tải lên: 25/10/2012, 10:51

... dishes and cultured at 33 °C (day 0 in Fig. 1). For the initial 2 weeks, primary cells, including both tsA58T Ag-negative cells (primarily fibroblasts) and tsA58T Ag-positive cells, proliferated, and ... isolation and maintenance of murine endothelial cells from various developmental stages and locations is important for dissecting molecular and cellular mechanisms of endo- thelial development and ... in these cells. Mes- enteric BECs and LECs (Fig. 5A,B) were also obtained by the same strategy as illustrated in Figs 1 and 4, and were treated with VEGF-A or VEGF-C (Fig. 5C). MAPK and Akt phosphorylation...

Ngày tải lên: 18/02/2014, 17:20

... detectable homology to CD40 ligand (data not shown) and that enhances adenoviral infection of CD40-positive cells of both human and murine origin, including DC and B cells resistant to infection ... as human and murine B cells. DC,LCL,L10A,Blasts:theselabels refer, respectively, to day 8 human DC, EBV-immortalized human B cells, murine B lymphoma L10A cells and murine LPS blasts. Cells (10 5 ) ... that binds to CD40 and enhances adenoviral infection of mouse and human DC and B lineage cells. Materials and methods Cell lines Epstein–Barr virus- (EBV-) transformed human B cells were provided...

Ngày tải lên: 20/02/2014, 11:20

... dopamine, R-(–)propylnorapomorphine, and raclopride did not affect oligomerization of D 2L and D 2S in Sf9 and HEK293 cells. Human D 2 dopamine receptors can therefore form consti- tutive oligomers in Sf9 cells and in HEK293 cells ... the dopamine D 2L and D 2S receptors can form constitutive homo- and hetero-oligomers in two expression systems (Sf9 and HEK293 cells) and these are not regulated by receptor ligands. Our study ... can also form hetero-oligomers in Sf9 cells. Time-resolved FRET with europium and XL665-labelled antibodies was appliedtowholeSf9 cellsandtomembranesfromSf9 cells expressing epitope-tagged D 2 receptors....

Ngày tải lên: 21/02/2014, 00:20

... to the asymmetric stretching of CH 3 ,and the band at 2 873 cm )1 to its symmetric mode. T he bands at 2926 and 2853 cm )1 can be a ssigned to an asymmetric and symmetric stretching mode of CH 2 , ... between 1300 and 900 cm )1 arise mainly from phosphate associated with nucleic acids, i.e. DNA and RNA. The a bsorption bands a t 1245 a nd 1087 cm )1 are c haracteristic o f asymmetric and symmetric ... plot of PCA-reduced s pectra of K5 62 cells. Resistant K562 cells (39 spectra, black stars), sensitive K562 cells (39 sp ectra, circles) o f the training (full) and test s et (empty). The percentage...

Ngày tải lên: 21/02/2014, 03:20

... 1626–9. 12 J. Aliberti NK cells, JG CD3 + T cells, noncytolytic NK cells, lymphoid tissue inducer cells, and skin-homing IL-13 + T cells. The receptor for IL-22 is distinct and consists of IL-22R, ... transmission. On the other hand, hosts need a robust and extended immune response in order to expand memory cells. This critical balance is where the co-evolution between host and pathogens lies. This ... activates NK cells to produce IFN-J and drives the proliferation of type 1 CD4 + and CD8 + T cells, which produce even more IFN-J. IFN-J-producing cells is a central component to induce and maintain...

Ngày tải lên: 05/03/2014, 23:20

... was detected in KM12, KM20L2 and WiDr cells. COX-1 was highly expressed in HT29 and WiDr cells and weakly expressed in KM20L2 cells. COX-2 was detected only in WiDr cells. The two terminal PGE 2 -biosynthetic ... mast cells, human mast cells developed in the presence of SCF and IL-6 from cord blood cells [38] expressed only sPLA 2 -IID, but not the other sPLA 2 s including -IB, -IIA, -IIE, -IIF, -V and ... by sPLA 2 -IIA and -V (net 4–5%) (Fig. 3A). When cells expressing sPLA 2 -IID were cocultured with those coexpressing COX-2 and mPGES and then stimulated (transcellular prostaglandin biosynthesis...

Ngày tải lên: 18/03/2014, 01:20

... H + /peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells Jana Neumann 1 , Mandy Bruch 1 , Sabine Gebauer 2 and Matthias Brandsch 1 1 Membrane Transport Group, Biozentrum and 2 Institute ... inhibition constants and the competitive nature of inhibition were confirmed in Dixon-type experiments. Caco- 2 cellsand SKPT cells were also cultured on permeable filters: apical uptake and transepithelial ... plus tracer binding, of 19.2% and 5.1% were determined with an excess amount of unlabeled Gly-Sar (31.6 m M Gly-Sar for Caco-2 cells and 20 m M Gly-Sar for SKPT cells) and subtracted from total uptake...

Ngày tải lên: 23/03/2014, 12:20

... conventional CD4 + and CD8 + T cells, and normal or increased development of CD4 + and CD8 + T cells, which have an activated memory-cell-like phenotype ([8–12], reviewed in [13] and [14]). These cells have ... devel- opment and function of iNKT cells and cd T cells seems to be quite different from conventional ab T cells. This minireview focuses on this aspect of Itk, its role in the development and function ... iNKT and NKT-like cd T cells. Itk and i NKT cell development NKT cells are a subset of innate T cells characterized by their expression of the NK1.1 marker along with the ab TCR. Although these cells...

Ngày tải lên: 28/03/2014, 22:21

... cord. NPFF-related peptides from cells and tissues were extracted and purified by RP-HPLC, assessed by radioimmunoassay and identified by using on-line capillary HPLC/nanospray ion trap tandem mass spectrometry ... at 94 °Cand30sat68°C. PCR products were cloned in pEGFPn3 (Clontech) and sequenced on both strands. Tissue extraction Mouse spinal cord tissue. Animals were handled in accordance with standard ... should help to understand their role in neurotransmision. Thus, we investigated the processing of proNPFF A in SH-SY5Y human neuroblastoma cells and COS-7 (nonneuronal) cells transiently transfected...

Ngày tải lên: 31/03/2014, 07:20

Ngày tải lên: 12/05/2014, 14:50

Ngày tải lên: 18/06/2014, 15:20

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 20/06/2014, 01:20

Ngày tải lên: 28/06/2014, 05:20

... between 48 and 60 h post-transfection. Blood cells and cell lines were labeled with calcein-AM (5 mL per 10 8 cells) for 30 min at 37 8C. Red blood cells, mixed white blood cells, Ramos and Daudi ... other blood cells including CD281 cells and CD31 cells (data not shown). The antibody was also used to probe Western blots of extracts from several cell lines and purified peripheral blood cells. The ... with adherent transfected COS7 cells, a binding assay with adherent endothelial cells was also performed. HUVEC cells (ATCC) were plated and grown to confluence in 96-well plates. COS7 cells were transfected...

Ngày tải lên: 24/03/2014, 04:21