REGENERATIVE MEDICINE AND TISSUE ENGINEERING - CELLS AND BIOMATERIALS Edited by Daniel Eberli Regenerative Medicine and Tissue Engineering - Cells and Biomaterials Edited by Daniel Eberli Published by InTech Janeza Trdine 9, 51000 Rijeka, Croatia Copyright © 2011 InTech All chapters are Open Access articles distributed under the Creative Commons Non Commercial Share Alike Attribution 3.0 license, which permits to copy, distribute, transmit, and adapt the work in any medium, so long as the original work is properly cited. After this work has been published by InTech, authors have the right to republish it, in whole or part, in any publication of which they are the author, and to make other personal use of the work. Any republication, referencing or personal use of the work must explicitly identify the original source. Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published articles. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials, instructions, methods or ideas contained in the book. Publishing Process Manager Romina Krebel Technical Editor Teodora Smiljanic Cover Designer Jan Hyrat Image Copyright Arun K. Sharma, 2011. First published August, 2011 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org Regenerative Medicine and Tissue Engineering - Cells and Biomaterials, Edited by Daniel Eberli p. cm. ISBN 978-953-307-663-8 Contents Preface IX Part 1 Regenerative Medicine 1 Chapter 1 Fundamental Technological Developments Required for Increased Availability of Tissue Engineering 3 Hideaki Kagami, Hideki Agata, Ryuji Kato, Fumiko Matsuoka and Arinobu Tojo Part 2 Cells for Regenerative Medicine 21 Chapter 2 Bone and Cartilage from Stem Cells: Growth Optimalization and Stabilization of Cell Phenotypes 23 Jan O Gordeladze, Janne E Reseland, Tommy A Karlsen, Rune B Jakobsen, Lars Engebretsen, Ståle P Lyngstadaas, Isabelle Duroux-Richard, Christian Jorgensen and Jan E Brinchmann Chapter 3 Mesenchymal Stem Cell-Based Bone Engineering for Bone Regeneration 57 Mohamadreza Baghaban Eslaminejad and Faezeh Faghihi Chapter 4 From Multipotent Cells to Fully Differentiated Connective Tissue Cells for Regenerative Medicine: Emerging Applications of Mesenchymal Stem Cells 83 Ali Mobasheri, Stephen M. Richardson, Judith A. Hoyland, Constanze Buhrmann and Mehdi Shakibaei Chapter 5 Skeletal Regeneration by Mesenchymal Stem Cells: What Else? 107 José A. Andrades, Silvia Claros, Pedro Jiménez-Palomo, José Mª López‐Puertas, Plácido Zamora‐Navas, Enrique Guerado, Manuel Monleón, María C. Araque and José Becerra Chapter 6 Production of Clinical Grade Mesenchymal Stromal Cells 145 Pytlík, Slanař, Stehlík and Matějková VI Contents Chapter 7 Adipose-Derived Stem Cells (ASCs) for Tissue Engineering 179 Mathias Tremp, Souzan Salemi, Rita Gobet, Tullio Sulser and Daniel Eberli Chapter 8 In Vitro Culture Methods of Skin Cells for Optimal Skin Reconstruction by Tissue Engineering 195 Moulin VJ, Mayrand D, Laforce-Lavoie A, Larochelle S and Genest H Chapter 9 Mesenchymal Stem Cells for Cell Therapy and Tissue Regeneration in Urology 209 Yingai Shi and Yuanyuan Zhang Chapter 10 Glandular Stem Cells: A New Source for Myocardial Repair? 229 Norbert W. Guldner, Charli Kruse and Hans - H. Sievers Part 3 Biomaterials for Regenerative Medicine 243 Chapter 11 Preparation and Characterization Urea-Solubilized Sol-Gel Type I Collagen and Its Possible Use in Applications 245 Xin Xiong, Herwig Brunner and Robin Ghosh Chapter 12 Direct Use of Resorbable Collagen-Based Beads for Cell Delivery in Tissue Engineering and Cell Therapy Applications 261 Veronica Glattauer, Wei-Bor Tsai, Jacinta F. White, Julie Nigro, Tracy A. Tebb, Jerome A. Werkmeister and John A.M. Ramshaw Chapter 13 Collagen: Applications of a Natural Polymer in Regenerative Medicine 287 Umber Cheema, Michael Ananta and Vivek Mudera Chapter 14 Tissue-Engineered Extracellular Matrices (ECMs) as Adjuvant Scaffolds for Endovascular Aneurysmal Repair (EVAR) 301 Anthony Callanan, Niall F. Davis, Michael T. Walsh and Tim M. McGloughlin Chapter 15 Elastin Based Constructs 323 Lisa Nivison-Smith and Anthony Weiss Chapter 16 The Use of a Hydrogel Matrix as a Cellular Delivery Vehicle in Future Cell-Based Therapies: Biological and Non-Biological Considerations 341 Thomas I. Zarembinski, William P. Tew and Sarah K. Atzet Contents VII Chapter 17 Cockle Shell-Based Biocomposite Scaffold for Bone Tissue Engineering 365 Zuki Abu Bakar, Bahaa F. Hussein and Noordin Mohamed Mustapha Part 4 Advanced Strategies 391 Chapter 18 Bioactive Scaffolds for the Controlled Formation of Complex Skeletal Tissues 393 Sandra Hofmann and Marcos Garcia-Fuentes Chapter 19 Angiogenesis and Vascularity for Tissue Engineering Applications 433 Remo A. Largo, Venkat M. Ramakrishnan, Martin Ehrbar, Algirdas Ziogas, Jan A. Plock and Daniel Eberli Chapter 20 The Liver Vascular Bed for Hepatocytes Cell Therapy and Tissue Engineering 449 Yaacov Baruch Chapter 21 Nano- Coating with Titanium of Glutaraldehyde- Fixed Heart Valve Prostheses Enables a Reduced Immune Response and a Self-Seeding Within Circulation 463 Norbert W. Guldner, Hangörg Zimmermann and Hans- H. Sievers Chapter 22 Formation of Stable Vascular Networks in Engineered Tissues 477 Bin Jiang and Eric M. Brey Chapter 23 Tunable Stimuli-Responsive Polymers for Cell Sheet Engineering 503 Nithya Joseph, Anil Kumar P R and TV Kumary Chapter 24 Gene-Silencing for Treatment of Cardiovascular Diseases 513 A. Nolte, M. Schneider, T. Walker and H. P. Wendel Chapter 25 Hydrodynamic 3D Culture for Bone Tissue Engineering 527 Dajiang Du, Takashi Ushida and Katsuko Furukawa Part 5 Cell - Biomaterial Interaction 549 Chapter 26 The Fibrotic Response to Implanted Biomaterials: Implications for Tissue Engineering 551 Barbara Rolfe, Jane Mooney, Bing Zhang, Sani Jahnke, Sarah-Jane Le, Yu-Qian Chau, Qiping Huang, Hao Wang, Gordon Campbell and Julie Campbell VIII Contents Chapter 27 Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds 569 Hsin-I Chang and Yiwei Wang . REGENERATIVE MEDICINE AND TISSUE ENGINEERING - CELLS AND BIOMATERIALS Edited by Daniel Eberli Regenerative Medicine and Tissue Engineering - Cells and Biomaterials. Regenerative Medicine and Tissue Engineering - Cells and Biomaterials, Edited by Daniel Eberli p. cm. ISBN 97 8-9 5 3-3 0 7-6 6 3-8 Contents Preface IX Part 1 Regenerative Medicine. and reliable serum-free cell culture media will be discussed with special reference to bone tissue engineering. 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