generation and characterization of isogenic cell lines expressing p53pro or p53arg
Regulation and functional analysis of the tumor suppressor gene product, p53
... dimers by p53 polymorphic 86 forms 3.2.8 Formation of micronucleic in p53Pro or p53Arg expressing cells 92 3.3 p53 polymorphism and melanin synthesis 96 VII 3.3.1 Expression of TRP-1 and Tyrosinase ... Prof Soo Khee Chee and A/Prof Hui Kam Man for providing me the opportunity to work in National Cancer Centre of Singapore I am really grateful and indebted to my parents for all of their support ... for 1min) X 35 cycles, 720C for 8min, Hold at 40C Size of the PCR product: 500 bp 2.7 Analysis of cell death Percentage of apoptotic cells and cell cycle were analyzed using the Fluorescence cell...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... injections of HBS-EP were also performed to assess noise, and to normalize injection data All samples were randomly injected for two minutes using the KINJECT command and the dissociation phase of binding ... fold of the reported LD 50 (1 μg of SEB) was observed which resulted in 20% mortality as compared to no mortality with μg of SEB alone (Figure 5A) A challenge of μg of SEB resulted in 100% mortality ... surface was performed by two injections of 10 mM HCl for 30 seconds at a flow rate of 100 μl/min The on- (ka) and off-rate (kd) of HuMAbs were determined by observing the signal over time for triplicate...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
... biology and Conrad and Dominik for the initial generation of 10.15 To Dr Lok Shee-Mei, Petra and Jiaqi, thank you for solving the cryo-EM structure of 14C10 and 10.15 To our collaborators at NUH and ... Strategies for the prevention and/ or treatment of dengue include vector control, development of a vaccine for DENV and specific therapeutics for DENV infections 1.2.5.1 Vector Control One of the most ... differentiating into memory B cells and antibody-secreting cells These B cells that secrete antibodies can be further separated into short-lived plasmablasts and longlived plasma cells Both memory and antibody-secreting...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
... Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka LIU RONG (Master of Biological Sciences) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF SCIENCE ... Sox2 and Nanog collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops in hESCs They identified 353 genes that were bound by all three transcription factors and ... genes for selfrenewal C-Myc has been reported to be the target gene of ERK or GSK3β, both of which are important downstream effectors for self-renewal (Fig 1-2) However, some of the intracellular...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... IL-13 and IL-25 (also known as IL-17E), which are important in the generation of the correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as helminths and ... against self- or foreign antigens [6,7] The majority of these cells constitutively express the CD25 cell surface marker and secrete two powerful anti-inflammatory cytokines: IL-10 and transforming growth ... internal control for RT-PCR PCR conditions were as follows: one cycle of 94 °C for min, 32 cycles of 94 °C for 30 s, 54 °C for 30 s and 72 °C for 30 s, followed by one cycle of 72 °C for PCR products...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) A 72 ... HaCaT cells were incubated with 100 lM each organic selenium compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell ... 0.05 Cytotoxicity of organic selenium compounds in human cells We examined the cytotoxic effects of selenoureas and selenoamides using three cell lines, HaCaT, HEL, and HEp-2, and PMNs, by the...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... Table The effect of inhibitors, inhibition pattern and enzyme-inhibitor dissociation constants for selected inhibitors of SAP1 and SAP2 I50 values, patterns of inhibition and Ki and Ki¢ values ... SAP2 by molybdate and fluoride was competitive and mixed, respectively (Table 4) Moreover, I50 and Ki values of SAP2 for molybdate, fluoride, and Pi were generally lower than the corresponding values ... suspension cells Tomato cells cultured for days in the absence of exogenous Pi had only approximately 40% of the fresh weight of the 8-day-old +Pi cells (approximately 63 and 25 g of cells were obtained...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
... where R ¼ A or G, Y ¼ C or T, K ¼ G or T, S ¼ C or G, W ¼ A or T, N ¼ A, G, C or T, and I ¼ deoxyindeoxyinosine The degenerate primers were employed in PCR amplifications of cDNA performed in a ... database, and a similar sequence was located The milk cell fraction of lactating tissue contains bud-off epithelial cells, enabling performance of an indirect assay for expression of this possible ... immediately after milking and stored on ice Milk cells were harvested by centrifugation at 3200 g for 20 at °C, and the cellular fraction was washed in NaCl/Pi buffer and processed for total RNA purification...
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Báo cáo y học: "Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine" doc
... surface of capsid, in their standard conformations Acknowledgements We thank National Foot -and- Mouth Disease Reference Laboratory of China, for providing FMDV isolates This work was supported by ... work and conserved in national foot -and- mouth disease reference laboratory Plaque assay and the pathogenicity in suckling-mice Confluent BHK-21 cell cultures in 6-well plates were prepared for ... altered receptor-ligand interactions, triggered by substitutions of residues in VP1, VP2 and VP3 Results Comparison of plaque phenotypes and infectivity of O/ Fujian/CHA/5/99, and O/Tibet/CHA/1/99...
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Báo cáo y học: "Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus" ppsx
... http://www.retrovirology.com/content/5/1/104 Figure Characterization of morphology of established cell lines Characterization of morphology of established cell lines Morphological appearance of the R1A, P8A, and ICR-A cell lines were ... and characteristics of cell lines Cell lines Cell culture Zpl 2-1 and C6 cell lines were used for the neuronal cell marker and the glial cell marker, respectively The neuronal cell line Zpl 2-1 ... function of the fact that the concentration of CAgag is higher in the P8 cell lines than in the R1 cell lines Release of CAgag protein and MuLV particles from R1A and P8A cell lines and analysis of...
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Báo cáo y học: " The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns" ppsx
... [4] Moreover, and like rodents, the dog develops spontaneous hepatocellular tumors However, these tumors are not associated with hepatitis and cirrhosis, and develop in normal livers Furthermore, ... majority of the cells; CK7 (Figure 3B) showed slight to moderate granular cytoplasmic staining in a minority of the cells As controls for the two stainings, liver tissue and kidney tissue of ... staining for hepatocyte marker HepPar1 strongly indicates the hepatocytic origin of the cultured cells [10] The neoplastic nature of the cells can be deduced from the pleiomorphism of the cells,...
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Báo cáo sinh học: " Establishment of stable Huh-7 cell lines expressing various hepatitis C virus genotype 3a protein: an in-vitro testing system for novel anti-HCV drugs" potx
... incubated for 15-30 Generation of stable cell lines of structural and non structural proteins After 72 hrs of transfection, cells were given selection with G418 initially with 400 ug/ml for selecting ... G418-resistant cell clones, which were picked after four weeks of culture and grown as individual cell lines Once the clones had been isolated and individually grown as cell lines, the concentration of neomycin ... initiated to establish stable cell lines expressing proteins of Pakistani HCV genotype 3a isolates The establishment of HCV genotype 3a cell lines stably expressing structural and non structural proteins...
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Characterization of MP2 cell division and pins function on spindle asymmetry of drosophila central nervous system
... cell Yet, in multicellular organisms, the progeny of that cell form a dazzling assortment of cell types Generation of this diversity relies on asymmetric divisions, in which the cell divides to ... localization and segregation of cell fate determinants 17 19 1.4.2 Adaptor proteins Miranda and Partner of Numb (Pon) direct the proper localization of cell fate determinants Pros and Numb, respectively ... DEPARTMENT OF ANATOMY & INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2006 ACKNOWLEDGEMENTS I would first of all like to thank my supervisor, Associate Professor Yang...
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Development and characterization of a SARS coronavirus replicon cell line
... definition of SARS A person with a history of: Fever (≥ 38°C) AND one or more symptoms of lower respiratory tract illness (cough, difficulty breathing, shortness of breath) AND radiographic evidence of ... (Delmas et al., 1990), is important for binding to cellular receptor and for mediating the fusion of viral and host membranes and thus is critical for virus entry into host cells (Collins et al., 1982; ... site of the protein and thus provided a useful platform for the rational design of effective inhibitors of this key enzyme Figure Overview of the domain organization and proteolytic processing of...
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Expression and characterization of FAT1 and atrophin 1 proteins regulating planar cell polarity and MBD1 protein involved in lymphoma
... organization of cells within the plane of a single layered sheet of cells i.e., the organization of groups of cells in the plane of the epithelium, such that they all orient to similar and apparently ... understanding of how cells and groups of cells become organized into higher order structures (Saburi, 2005).One form of higher organization that is currently under intense investigation is planar cell polarity ... class of nuclear receptors Nuclear receptors (NRs) comprise one of the largest known families of eukaryotic transcription factors (Mangelsdorf, 1995) The majority of identified NRs are ‘orphan’...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the flocculating ... treatment of extracellular polymer The extracellular polymer was dissolved in distilled water at a concentration of 0.5 mg/ml and heated at 100°C for The estimation of flocculating activity for kaolin...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... 3a, Figure 3b and Figure 3c show the C1s core-level spectra, O1s corelevel spectra and S2p core-level spectra of the PVDF-SPS membrane, respectively The C1s core-level spectrum of the PVDF-SPS ... polybenzimidazole and its composites with inorganic proton conductors J Membr Sci, 2003, 226(1-2):169-184 [15] C W Lin, K C Fan, R Thangmuthu Preparation and characterization of high selectivity organicinorganic ... time for PVDF-SPS membrane and Nafion-117 membrane were measured Figure shows that the two kind of membranes report a linear relationship between CB and t, and the methanol permeability of the...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... system is capable of producing off-resonance, mirror, and flat magnetic field configurations A mirror ratio of 1·1 and a maximum flat field of 1400 G has been obtained The measured values and the design ... values of NI for the side and middle solenoid coils were obtained to be 12950 and 8250 respectively Based on these parameters, the total length of the conductor was calculated A copper conductor ... Poisson software for (a) mirror magnetic field, and (b) flat magnetic field are shown in figure The calculation was also performed analytically (Montgomery 1966) using standard relations for calculating...
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Tài liệu Báo cáo "Preparation and characterization of nanocomposite TiO2/SnO2 films " pdf
... prepared via two steps of spray pyrolysis for TiO2 and sol-gel for SnO2 As prepared films exhibit a nanocomposite structure, constituting of a majority of TiO2 and a small amount of SnO2 located on ... work, SnO2 was formed from SnCl4 during annealing impregnated TiO2 film Therefore photoeffect of impregnated TiO2 films increases according to time and temperature of annealing In the other hand, ... temperature in order to form SnO2 For the best results, impregnations were carried out by varying concentration of the SnCl4 solution, and the films were annealed at different temperatures and for different...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... alignment of GmPDIL-3a, GmPDIL-3b and orthologs of other plants Fig S3 Expression and CD analysis of recombinant GmPDIL-3a and GmPDIL-3b Table S1 List of primers for ATG-mutagenesis of GmPDIL-3a and ... Promotion of Basic Research Activities for Innovative Biosciences, and a Grant-in-Aid for Exploratory Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan ... Ero1a and Ero1b into the ER by forming a mixed disulfide bond and by controlling the ratio of redox isoforms of Ero1a [12,38,39] Instead, GmPDIL-3a and GmPDIL-3b may function as retention or redox...
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