generation and characterization of isogenic cell lines expressing p53pro and p53arg
Regulation and functional analysis of the tumor suppressor gene product, p53
... Expression of p53 and its target genes in isogenic stable cell line 69 14 Apoptosis in isogenic stable cell line 70 15 Expression of Gadd45 and p48 in isogenic cell line 72 16 Expression of p53R2 in isogenic ... stable 67 cell lines 3.2.4.1 Apoptosis induce by temperature sensitive p53- isogenic cell lines 68 3.2.4.2 p53 dependent DNA-repair genes are differentially regulated 68 by p53Pro- and p53Argexpressing ... Regulation of p53 dependent DNA-repair target gene promoters 60 by p53Pro and p53Arg variants VI 3.2.2.1 Expression of Gadd45 promoter in presence of either p53Pro 60 or p53Arg 3.2.2.2 Expression of...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... regulations and guidelines of the National Institutes of Health (NIH) and with the approval of the TPPS Animal Care and Use Committee Mice were challenged with various concentrations of SEB (Toxin ... capable of neutralizing high doses of SEB both in vitro and in vivo Our lead antibody HuMAb154 was selected for further characterization because of its high affinity and in vitro high potency and ... to confirm retention of SEB specificity Light and heavy chain genes of lead clones were sequenced and cloned for recombinant expression of corresponding antibodies in CHO cells HuMAb ELISA reactivity...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
... biology and Conrad and Dominik for the initial generation of 10.15 To Dr Lok Shee-Mei, Petra and Jiaqi, thank you for solving the cryo-EM structure of 14C10 and 10.15 To our collaborators at NUH and ... Figure 23 Binding activity of 10.15 to various strains of DENV2 and DENV1, and 101 Figure 24 Binding activity of 12.17 to various strains of DENV2 and DENV1, and 102 ... Philippines and recent isolates from South Pacific islands Thailand, Vietnam and Bangladesh Sri Lanka, India, Africa, Samoa and 1962 strain from Thailand Puerto Rico, Latin and central America and 1965...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
... Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka LIU RONG (Master of Biological Sciences) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF SCIENCE ... eukaryotic cells and expression analysis in vitro 60 2.2.5.1 Construction of plasmid of DNA 60 2.2.5.2 Cell culture 61 2.2.5.2.1 Culture conditions 61 2.2.5.2.2 Freezing and thawing of the cells 62 ... Developmental potency of stem cells Stem cells are unspecialized cells that have the capability of self-renewal for producing identical unspecialized daughter cells and the potential of differentiation...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... IL-13 and IL-25 (also known as IL-17E), which are important in the generation of the correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as helminths and ... been seen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also ... expression of t-bet, stat6 and foxp3 was observed in the spleen, kidney, gill, gut, liver and skin tissue of healthy fish (data not shown) After stimulation of kidney cells with a variety of immunostimulants,...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) A 72 ... HaCaT cells were incubated with 100 lM each organic selenium compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell ... against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro Results...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... 8-day-old +Pi cells (approximately 63 and 25 g of cells were obtained per 500 mL culture of 8-day-old +Pi and –Pi cells, respectively) Eight days following subculture of the tomato cells into –Pi ... Influence of Pi starvation on growth and secreted AP activity of tomato suspension cells Tomato cells cultured for days in the absence of exogenous Pi had only approximately 40% of the fresh weight of ... R-250 The migration of various Mr standards is shown on the left TD, tracking dye front (C) Lanes and of this nondenaturing gel contain 0.5 lg of the final preparation of SAP1 and SAP2, respectively...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
... database, and a similar sequence was located The milk cell fraction of lactating tissue contains bud-off epithelial cells, enabling performance of an indirect assay for expression of this possible ... PCR screening of bovine and human cDNA libraries showed the presence of MUC15 mRNA in human breast, bovine mammary gland, bovine lymph nodes and lungs of both species (Table 1) Of the identified ... Expression of MUC15 in human and bovine tissues and cell types MUC15 mRNA expression was examined by PCR screening of commercial multiple tissue cDNA panels and oligo(dT) primed cDNA libraries and by...
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Báo cáo y học: "Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine" doc
... attenuation of these viruses Y79 H within bC strand and E136G within E-F loop of VP2, T142N/A and A152T within G-H loop of VP1 are exposed on the surface of the viral capsid The direct induction of capsid ... accumulating over the course of FMD outbreaks with infection of susceptible animals, is contributed to the understanding of the occurrence of phenotypic changes in cultured cells and alteration in host ... clinical signs in an infection of Holstein cattle, and sheep and goats were not susceptible [16] Comparison of amino acid sequence of structural proteins of two Page of 11 different plaque phenotypes...
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Báo cáo y học: "Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus" ppsx
... http://www.retrovirology.com/content/5/1/104 Figure Characterization of morphology of established cell lines Characterization of morphology of established cell lines Morphological appearance of the R1A, P8A, and ICR-A cell lines were ... and characteristics of cell lines Cell lines Cell culture Zpl 2-1 and C6 cell lines were used for the neuronal cell marker and the glial cell marker, respectively The neuronal cell line Zpl 2-1 ... in growth rates of R1A, P8A and ICR-A cell lines The difference in growth rates of R1A, P8A and ICR-A cell lines (A) Comparison of the growth rates of R1A, P8A, and ICR-A cell lines (B) Representative...
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Báo cáo y học: " The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns" ppsx
... smaller clusters and not as single cells The cell clusters were collected, fixed and handled as described As shown in Figure 2, histology revealed solid cell clusters of large epithelial cells, with ... origin of the cultured cells [10] The neoplastic nature of the cells can be deduced from the pleiomorphism of the cells, the number of sometimes bizarre mitotic figures The simultaneous presence of ... presence of CK7 and HepPar1 positive cells is also consistent with the neoplastic nature of the cells [11], and suggests the presence of both fully differentiated hepatocytes and progenitor cells in...
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Báo cáo sinh học: " Establishment of stable Huh-7 cell lines expressing various hepatitis C virus genotype 3a protein: an in-vitro testing system for novel anti-HCV drugs" potx
... G418-resistant cell clones, which were picked after four weeks of culture and grown as individual cell lines Once the clones had been isolated and individually grown as cell lines, the concentration of neomycin ... initiated to establish stable cell lines expressing proteins of Pakistani HCV genotype 3a isolates The establishment of HCV genotype 3a cell lines stably expressing structural and non structural proteins ... dissolved in 1XPBS and blot was incubated for 15-30 Generation of stable cell lines of structural and non structural proteins After 72 hrs of transfection, cells were given selection with G418 initially...
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Characterization of MP2 cell division and pins function on spindle asymmetry of drosophila central nervous system
... Asymmetric cell division versus symmetric cell division Every organism begins as a single cell Yet, in multicellular organisms, the progeny of that cell form a dazzling assortment of cell types Generation ... models of asymmetric cell divisions Fig 1.2 Polarized distribution of proteins and displacement of mitotic spindle in C.elegans P0 division Fig 1.3 Delamination and asymmetric cell division of Neuroblasts ... localization and segregation of cell fate determinants 17 19 1.4.2 Adaptor proteins Miranda and Partner of Numb (Pon) direct the proper localization of cell fate determinants Pros and Numb, respectively...
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Development and characterization of a SARS coronavirus replicon cell line
... Synthesis of SARS-CoV first-strand cDNA by reverse transcription 31 2.2.3 Synthesis of A DNA 32 2.2.4 Synthesis of B, C and N, and GFP-BlaR gene DNAs 33 2.2.5 Assembly and amplification of BCGbN ... Assembly of ABCGbN DNA 36 2.2.7 Synthesis of SARS-CoV replicon RNA 37 2.3 Development of SARS-CoV replicon-carrying cell lines 38 2.3.1 Maintenance of BHK-21 Cell Line 38 2.3.2 Transfection of BHK-21 ... Detection of GFP-BlaR gene in total cell DNA 47 2.4.4.1 Extraction of total cell DNA 47 2.4.4.2 PCRs for the detection of GFP-BlaR and GAPDH genes 47 iii 2.4.5 Sequencing of SARS-CoV replicon and sub-replicon...
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Expression and characterization of FAT1 and atrophin 1 proteins regulating planar cell polarity and MBD1 protein involved in lymphoma
... www.postech.ac.kr/life) 15 Planar cell polarity is the coordinated organization of cells within the plane of a single layered sheet of cells i.e., the organization of groups of cells in the plane of the epithelium, ... 41 Cloning and Expression of MBD1 3.5.1 Cloning of MBD1 41 3.5.2 Expression of MBD1 42 CHAPTER RESULTS AND DISCUSSION vi 4.1 Cloning of Fat1 and Atrophin1 43 4.2 Expression of Fat1 and Atrophin1 ... 4.8 Purification of Fat1 sing TALON resin 51 Figure 4.9 FPLC profile of Fat1 51 Figure 4.10 DLS and native gel profile of Fat1 53 Figure 4.11 Mass determination and verification of Fat1 54 Figure...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... digestion and heat treatment of extracellular polymer Initially, 16.8 mg of pronase-digested extracellular polymer was obtained from 40 mg of the intact polymer Figure 1B shows the elution profile of ... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... diameter of the PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane is 0.25 µm, 0.12 µm and 0.16 µm, respectively It can be seen that the polymerization of styrene and the blending of PS and ... (DMAc) firstly, and then styrene, divinylbenzene (DVB) and benzoyl peroxide (BPO) were added to the solution and stirred with water bath at 343 K The dosage of PVDF, styrene, DVB and BPO was that ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 645 2.5 Measurement of proton conductivity The proton conductivity of the PVDF-SPS membrane and Nafion-117...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... system is capable of producing off-resonance, mirror, and flat magnetic field configurations A mirror ratio of 1·1 and a maximum flat field of 1400 G has been obtained The measured values and the design ... varying the size of the solenoid coils, thickness of iron jacket, and amp-turns (NI) The optimum values of NI for the side and middle solenoid coils were obtained to be 12950 and 8250 respectively ... fringing magnetic field and to reduce the power consumption The use of jacket is to provide a return path for the magnetic field lines and thereby shield the adjacent components The use of electromagnet...
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Tài liệu Báo cáo "Preparation and characterization of nanocomposite TiO2/SnO2 films " pdf
... evaporation of solvents and volatile products, took place simultaneously with the deposition process, caused the porosity of the TiO2 films Prepareation of TiO2/SnO2: After the material characterization ... concentration of the SnCl4 solution, and the films were annealed at different temperatures and for different period of time Photoconductivity measurement In order to evaluate properties of obtained ... resistance (RD) and the light resistance (RL) under the irradiation of 7W Hg lamp at the distance of 10cm were measured The calculated ratio of RD/RL was considered as a photoconductivity of obtained...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... Identification of the initiation codons in GmPDIL-3a and Gm-PDIL-3b mRNAs (A) Schematic representation of the structure of GmPDIL-3a and GmPDIL-3b mRNAs The putative ORFs (gray boxes) of GmPDIL-3a and GmPDIL-3b ... S1 Comparison of intron–exon structures of GmPDIL-3a, GmPDIL-3b and orthologs of other plants Fig S2 Multiple amino acid sequence alignment of GmPDIL-3a, GmPDIL-3b and orthologs of other plants ... and CD analysis of recombinant GmPDIL-3a and GmPDIL-3b Table S1 List of primers for ATG-mutagenesis of GmPDIL-3a and GmPDIL-3b This supplementary material can be found in the online version of...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25