excitation contraction coupling functional properties and autonomic and hormonal regulation in human embryonic stem cell derived cardiomyocytes
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... culture on a human ECM, maintained on chemically defined culture medium containing only human substances and proteins to produce clinical-grade hESCs [16] And such a human ECM can made by human fibroblasts, ... factors and anchoring substrates are the underlying principles of these systems, which try to mimic the microenvironments of the hESCs Indeed, keeping in mind the origin of hESCs, the neighbouring cells ... would in nature, and in similar proportions to that of in vivo ECM is formed from many different structural macromolecules and plays a crucial role in maintaining tissue structure and modulating cell...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, based on work by Hughes et al 2009 † Murine protein is a homolog of human protein 50 ... metalloproteinase with thrombospondin motifs 1*† Transforming growth factor-beta-induced protein ig-h3 74665 122841 234698 Isoform of Extended synaptotagmin-1*† Isoform of Myoferlin*† 99.80% ... 105340 Keratin, type I cytoskeletal 10 Keratin, type II cytoskeletal Collagen alpha-1(I) chain*† Collagen alpha-2(I) chain*† EMILIN-1*† Fibrillin-1* Isoform of Collagen alpha-1(XII) chain Isoform...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... contains more Fn (by immunofluoresence comparison, section 3.3.2), it would be expected that the 52 increased presence of Fn would aid in increasing integrin binding and hence cell binding This ... due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs, since DCN and BGN both bind active TGFB, and could lead ... amounts of proteins within and their complexity (refer to section 3.3), not allow the adherence of hESCs and also not maintain hESCs in a pluripotent state This observation is surprising, since these...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
... of hESCs, and so, in order to obtain successful Oct-4 immunostaining, the cells have to be first permeabilized using 0.2% Triton-X 100 - this extra processing step results in markedly increased ... trophoectoderm lineages [10], indicating the crucial role that Oct-4 plays in maintaining the pluripotent state of ES cells As Oct-4 is a transcription factor, naturally it is found within the nuclei ... down-regulated in trophoectoderm and primitive endoderm of the mouse embryo Later, it is maintained in the embryonic ectoderm at the cylinder stage, and down-regulated at gastrulation in an anterior-posterior...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
... at increasing the population doublings and also in 84 maintaining the pluripotent characteristics of the hESCs during their propagation Once again, hESCs were cultured onto Matrigel, DxSDOC and ... Stem Cell Bank In this protocol, the hESCs formed embryoid bodies using non-adherent cell culture plates and were transferred onto laminincoated plates and incubated in neural induction medium ... In vitro induced differentiation hESCs cultured on DxSDOCDOC for 13 passages were induced into neural differentiation using a protocol modified from WiCell Research Institute, National Stem Cell...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
... Kaukinen K, Maki M, Lindfors K Extracellular transglutaminase has a role in cell adhesion, whereas intracellular transglutaminase is involved in regulation of endothelial cell proliferation and ... possibility of developing an alternative embryonic stem cell culture system, with the aim of overcoming current obstacles that hinder the safe clinical implementation of human embryonic stem cell culture ... facilitate cell growth by promoting cell signaling and growth factor availability However, subsequent staining and gel electrophoresis also showed that in addition to Fn and collagen, which aids in cell...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... characterized, leaving a gap in our understanding of their molecular interactions with target cells and, thus, their role during early embryonic patterning and cell lineage commitment This is due, in part, ... et al and GFAP Mouse kidney and small intestine were used as control tissues for Ksp-cadherin or E-cadherin and pan-cyokeratin staining, respectively Sudan Red and Alizarin Red S stainings Sudan ... in the BMP2+ cell population since it includes proliferating NCSCs on the one hand (increased cell number when subjected to immunostaining) and melanocytes in the same culture on the other hand...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... lncRNAs involved in differentiation has been carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem cells ... pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, and can be maintained undifferentiated in culture Pluripotency and self-renewal are hallmarks of embryonic stem ... demonstrating the pivotal role of transcription factors in the maintaining and inducing the pluripotent cell state 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... target 226 lincRNAs expressed in mESCs, and the authors identified 26 lincRNAs that resulted in loss of Nanog and Oct4 expression upon lincRNA knockdown, indicating that lincRNAs maintain the pluripotent ... governing stem cell pluripotency and cell fate determination, and perturbations of lncRNA levels will result in stem cell differentiation 104 Chapter VII – Long Non-coding RNAs are Indispensable in ... lncRNAs using anti-SUZ12, anti-SOX2 and anti-OCT4, respectively lncRNA_ES1 and lncRNA_ES2 were enriched in the SUZ12 RIP and SOX2 RIP, indicating interaction * and ** indicate p-values of < 0.05 and...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... qPCR in two independent cell lines: hESC (H1) -derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC -derived cells and ReN-VM cells, RMST expression was upregulated by approximately ... database indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line, ... expressed in the midbrain and prospective dopamine neurons Rmst transcript was detected by in situ hybridization in the mouse embryonic brain (A, B) Rmst as detected in both thr roof plate (RP) and...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic stem cells (hESCs), and ... is a RNAbinding protein and probably binds directly to RMST SOX2 and hnRNPA2B1 may interact via protein-protein interactions The transcription factor SOX2 may then bind to target chromatin regions ... reprogramming of mouse and human somatic cells to pluripotency Cell Stem Cell 8, 376-388 Anthony, T.E., Klein, C., Fishell, G., and Heintz, N (2004) Radial glia serve as neuronal progenitors in all...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... PAX6a contains two DNA-binding domains, which includes the canonical N-terminal paired domain (PD) comprised of 128 amino 13 acid resides and a homeodomain (HD), and a proline-serine-threonine-rich ... deciphering the mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell ... Glial cell- derived nerve factor Glial fibrillary acidic protein Glial progenitor cell Homeodomain Human embryonic stem cell High mobility group Herpes simplex virus thymidine kinase Interferon Interleukin...
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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt
... J, Skalnik DG: Reduced genomic cytosine methylation and defective cellular differentiation in embryonic stem cells lacking CpG binding protein Molecular and cellular biology 2005, 25:4881-4891 ... genes and epigenetic remodeling in stem cell biology is needed, the current results are a great step forward in establishing the feasibility and applicability of adult stem cells in future clinical ... toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem cells (ESC), mesenchymal stem cells (MSC), and various tissues using all...
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báo cáo hóa học: " Function, Adjustment, Quality of Life and Symptoms (FAQS) in Allogeneic Hematopoietic Stem Cell " potx
... improvement in functioning between and years post-transplant [35] In contrast, particularly in patients older than 25 at the time of HSCT, recovery may be non-linear, with functional status continuing ... address an important gap in understanding by offering a prospective, longitudinal, multidimensional examination of functional status, psychosocial adjustment, HRQL, and symptoms in a cohort of survivors ... analyses Covariates, including biologic, physiologic, and treatment-related factors, are anticipated to influence HRQL and will be incorporated into the cross-sectional and longitudinal analysis as...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
... target cells that need to be protected against HIV-1 infection Methods hES cell growth and propagation H1 human embryonic stem cell line (hES) was obtained from WiCell (Madison, Wisconsin) The ... Research and Therapy 2008, 5:1 demonstrated the ability of hES cells to differentiate into a myriad of cell types that include neurons, hematopoietic cells, cardiomyocytes, and insulin-secreting cells, ... Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonic stem cell lines from human blastocysts:...
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Dual roles of transcription factor zic3 in regulating embryonic stem cell pluripotency and differentiation
... Introduction 1.1 Derivation of embryonic stem cells 1.2 Regulation of embryonic stem cells 1.2.1 The key properties of ES cells 1.2.2 Extrinsic signalling pathways maintaining ES cell ... signalling pathways maintaining ES cell pluripotency Embryonic stem cells are maintained by a network of extrinsic and intrinsic signals that collectively regulate the properties of pluripotency and ... from cell lines Cell culture Mouse ES cell culture Human ES cell culture Isolation, expansion, and mitotic inactivation of MEF cells Maintenance of HEK293T cells Cryopreservation of cell lines...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... - GA Growth derived factor - GDF Haematoxylin and eosin - H&E Hours - hr Human- specific vimentin - hVIM Human embryonic stem cells - hESCs Human ESC -derived chondrogenic cell- engineered cartilage ... sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells (hESCs) are stem cell lines of embryonic origin, isolated from the inner cell mass of the blastocyst-stage ... such as the BMPs and TGFβs, not only play a prominent role in driving embryonic patterning and cell commitment events, but are also involved in the regulation of limb development and cartilage formation...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable ... Immunoflourescent staining of neural stem cell marker Nestin in SHH-CM and Control-CM treated EB Middle panel shows corresponding DAPI nuclear staining in blue and right panel shows corresponding merged...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... self-renewing phenotype, as Oct2 and Oct6 were found to have no effect on cell fate in this system Intriguingly, truncated Oct4 protein containing the Oct4 POU domain and either the C- or N-terminal ... Sox2 DNA interactions, characterized the joint DNA motif, expanded the Oct4 and Sox2 transcription binding network to the Smad1 and Stat3 signalling networks and utilized these binding data in an ... αOct4 and αSox2 antibodies used in ChIP 3.2 Oct4 and Sox2 binding to Oct4 CR4 region in mESCs 3.3 Oct4 and Sox2 binding reduces after retinoic acid differentiation of mESCs 3.4 Oct4 and Sox2 bind...
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