DxSDOC Subcellular Location Accession # Cytoplasm IPI00216587, IPI00645201 IPI00021439, IPI00894365, IPI00894498 IPI00025416 Molecular Weight (Da) 24188 41720 41860 IPI00013452 170575 IPI00152692 23406 IPI00396485, IPI00472724 IPI00297982 50123 IPI00219018, IPI00789134, IPI00795257 IPI00414676 36035 IPI00141318, IPI00604713, IPI00883950, IPI00939370 IPI00003865, IPI00037070, IPI00939595 66004 51092 83249 70882 Protein Name Identification Probability 40S ribosomal protein S8* Actin, cytoplasmic 1* Actin, gamma-enteric smooth muscle* Bifunctional aminoacyltRNA synthetase* D-tyrosyl-tRNA(Tyr) deacylase Elongation factor 1alpha 1* Eukaryotic translation initiation factor subunit Glyceraldehyde-3phosphate dehydrogenase*† Heat shock protein HSP 90-beta* Isoform of Cytoskeleton-associated protein 4*† Isoform of Heat shock cognate 71 kDa protein*† DxSDOCDOC % Coverage Protein name Identification Probability 100.00% # of Unique Peptides % Coverage 99.80% # of Unique Peptides 18.80% 100.00% 28.30% 40S ribosomal protein S8* Actin, cytoplasmic 1* 100.00% 36.50% 99.80% 17.30% 99.80% 2.18% 99.80% 14.80% D-tyrosyl-tRNA(Tyr) deacylase Elongation factor 1alpha 1* 99.80% 14.80% 100.00% 14.30% 99.80% 5.63% 99.80% 7.20% 100.00% 20.00% Glyceraldehyde-3phosphate dehydrogenase*† 99.80% 8.66% 99.80% 5.25% 100.00% 16.30% Isoform of Cytoskeletonassociated protein 4*† 99.80% 4.82% 100.00% 9.13% 13.50% 46 IPI00019502 226520 Isoform of Myosin-9* 100.00% 16 12.90% IPI00302592, IPI00333541, IPI00644576 IPI00043863, IPI00220113, IPI00396171, IPI00411375, IPI00743873, IPI00878314, IPI00878651, IPI00880007, IPI00888475, IPI00924603, IPI00927173, IPI00927819 IPI00465248, IPI00759806 IPI00479186, IPI00847989 279991 Isoform of Filamin-A* 100.00% 14 8.72% 88260 Isoform of Microtubule-associated protein 100.00% 5.19% 47152 99.80% 6.68% 99.80% 6.59% IPI00000105, IPI00910019 IPI00008868 99308 Isoform alpha-enolase of Alpha-enolase* Isoform M2 of Pyruvate kinase isozymes M1/M2* Major vault protein*† 100.00% 10.10% Microtubule-associated protein 1B*† TUBA1C protein 100.00% 3.04% 99.80% 10.10% Vimentin* 100.00% 11.60% IPI00166768, IPI00180675, IPI00218343, IPI00387144, IPI00792677, IPI00793930, IPI00909762, IPI00930688 IPI00418471 IPI00215743, 57920 270602 36725 53635 152453 Isoform of Myosin9*† Isoform of FilaminA* 100.00% 2.81% 100.00% 1.10% Isoform of 100.00% 3.33% 47 Extracellular Matrix IPI00220967, IPI00744135, IPI00856098 IPI00009865 Ribosome-binding protein 58811 IPI00220327 66022 IPI00297646 138923 IPI00304962 129297 IPI00013079 IPI00328113 IPI00329573, IPI00964552 IPI00022200, IPI00072918, IPI00220701 IPI00022418, IPI00339223, IPI00339224, IPI00339225, IPI00339226, IPI00339227, IPI00845263, IPI00855785 IPI00031008, IPI00220213 IPI00294578 106649 312268 333127 IPI00304840 108563 IPI00296099 IPI00298971 IPI00005908 129364 54288 105340 Keratin, type I cytoskeletal 10 Keratin, type II cytoskeletal Collagen alpha-1(I) chain*† Collagen alpha-2(I) chain*† EMILIN-1*† Fibrillin-1* Isoform of Collagen alpha-1(XII) chain Isoform of Collagen alpha-3(VI) chain*† 100.00% 5.31% 99.80% 3.42% 100.00% 5.74% 100.00% 5.49% 100.00% 99.80% 100.00% 21 6.40% 0.94% 10.10% 100.00% 10 5.23% Collagen alpha-1(I) chain*† Collagen alpha-2(I) chain*† EMILIN-1*† Fibrillin-1* Isoform of Collagen alpha-1(XII) chain Isoform of Collagen alpha-3(VI) chain*† 100.00% 4.10% 100.00% 8.57% 100.00% 100.00% 100.00% 23 8.17% 1.25% 11.80% 100.00% 15 7.84% 262599 Isoform of Fibronectin*† 100.00% 21 14.60% Isoform of Fibronectin*† 100.00% 15 8.51% 240832 Isoform of Tenascin*† 100.00% 5.77% 100.00% 4.54% 77311 Isoform of Transglutaminase Isoform 2C2 of Collagen alpha-2(VI) chain Thrombospondin-1* Vitronectin* 100.00% 12.40% Isoform of Tenascin*† Isoform of Transglutaminase 100.00% 10.50% 99.80% 4.61% 100.00% 99.80% 8.29% 8.79% Thrombospondin-1* 100.00% 6.50% A disintegrin and 100.00% 4.96% 343645 48 Membrane Nucleus IPI00018219, IPI00873923, IPI00940091 IPI00022143, IPI00746655 IPI00021048, IPI00216268, IPI00216269, IPI00645867 IPI00219365, IPI00872814 IPI00217466 IPI00217468 IPI00021924 IPI00021405, IPI00216952, IPI00644087, IPI00655812 IPI00021812 metalloproteinase with thrombospondin motifs 1*† Transforming growth factor-beta-induced protein ig-h3 74665 122841 234698 Isoform of Extended synaptotagmin-1*† Isoform of Myoferlin*† 99.80% 2.54% 100.00% 1.89% 67804 Moesin* 100.00% 7.45% 22333 22564 22470 74123 Histone H1.3 Histone H1.5 Histone H1x* Isoform A of PrelaminA/C 100.00% 100.00% 99.80% 100.00% 3 14.50% 14.20% 14.10% 11.30% 629086 Neuroblast differentiationassociated protein AHNAK*† Peptidyl-prolyl cis-trans isomerase B*† 100.00% 2.36% 99.80% 12.00% IPI00646304 23725 IPI00018534, IPI00020101, IPI00152906, IPI00303133, IPI00329665, IPI00419833, IPI00477495, IPI00554798, IPI00646240, 13935 100.00% 4.69% Histone H1.3 Histone H1.5 100.00% 100.00% 3 17.60% 13.70% Isoform A of Prelamin-A/C 100.00% 9.04% Histone H2B type 1-L 99.90% 20.60% 49 Unknown IPI00794461, IPI00815755, IPI00930174, IPI00930570 IPI00022431, IPI00795830, IPI00922262, IPI00953689 IPI00376689 IPI00022434, IPI00745872, IPI00966829 46608 cDNA FLJ55606, highly similar to Alpha-2-HSglycoprotein 99.80% 3.00% 152983 Isoform of Protein KIAA1199 Putative uncharacterized protein ALB 99.80% 1.91% 100.00% 7.02% 71687 cDNA FLJ55606, highly similar to Alpha-2-HSglycoprotein 99.80% 3.00% Putative uncharacterized protein ALB 100.00% 7.81% Table 2: Consolidated list of protein identifications compiled from mass spectrometric analysis of DxSDOC and DxSDOCDOC matrices. Mass spectrometry analysis was done in collaboration with Christopher Stephen Hughes and Prof Gilles Lajoie. * Protein also found in GFR and standard Matrigel, based on work by Hughes et. al. 2009. † Murine protein is a homolog of human protein 50 . analysis was done in collaboration with Christopher Stephen Hughes and Prof Gilles Lajoie. * Protein also found in GFR and standard Matrigel, based on work by Hughes et. al. 20 09. † Murine. Isoform 2C2 of Collagen alpha -2( VI) chain 99.80% 2 4.61% IPI0 029 6099 129 364 Thrombospondin-1* 100.00% 7 8 .29 % Thrombospondin-1* 100.00% 6 6.50% IPI0 029 8971 5 428 8 Vitronectin*. Transforming growth factor-beta-induced protein ig-h3 100.00% 2 4.69% Membrane IPI00 022 143, IPI00746655 122 841 Isoform 1 of Extended synaptotagmin-1* † 99.80% 2 2. 54%