electric fields at the active side of an enzyme direct comparison of experiment with theory 200
Báo cáo khoa học: Site-directed mutagenesis of selected residues at the active site of aryl-alcohol oxidase, an H2O2-producing ligninolytic enzyme pot
... AAO, comparison of the F501A and F501Y variants suggested that this residue could modulate the redox potential of the FAD, affecting the enzyme kcat and electronic absorption spectrum, rather than ... spectra of the Y78A, Y92F and F501Y variants were very similar to that of wild-type AAO (Fig 3A) with absorption maxima at 387 and 463 nm, indicating that the cofactor was in the oxidized state and ... nidulans cultures AAO was secreted by E nidulans, and the activities of the site-directed variants (when active) could be directly detected in filtrates of 48 h cultures of the transformants harbouring...
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Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx
... with E1 was incubated for exactly 10 at the relevant temperature and the catalytic activity at that temperature was then determined The inactivation temperatures for all the E1 mutants were found ... by any of the mutations in E1a (Fig 4) Therefore, any effects of the mutations on the catalytic activities of the PDH complex must be due to direct effects on the reactions catalysed by E1 The ... binding the 2-oxo acid substrate and that E1aTyr281 and, to a lesser extent, E1aAsp276 and E1aArg282, have some effect on the decarboxylation of pyruvate and the reductive acetylation of the tethered...
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Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
... affinity of MgATP2) [11,12], which is compatible with a direct interaction of these residues with the nucleotide at the active site The catalytic mechanism of GK has been the subject of several ... of that related to the catalytic mechanism of yeast hexokinase [17] For both enzymes, part of the discussion has been related to the question of whether ATP binds to the Glc-free enzyme and the ... of a nucleotide-triggered change in protein conformation In this work, we have studied the interaction of ATP and analogues with the human pancreatic enzyme with the aims of: (a) presenting experimental...
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Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase pptx
... substrate binding (Fig S1) [15] On the other hand, the hydrolysis rate varies with the substituent on the amino moiety of the substrate, confirming that the formation of acyl enzyme (AE) is the rate-limiting ... substrate, and the phenylmethanesulfonyl-SerB1 derivative These structures are mimics of the stationary points along the reaction pathway; they depict the changes in the spatial structure of the active ... stabilization by a protonated amino group in the proximity of the oxyanion can be found in the rest of the Ntn hydrolases [3] From another point of view, the hydrogen bond between the oxyanion and the...
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Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... conformational states (isomers) of the enzyme The four conformational states include a ground state for the ligand-free enzyme, an activated state with bound substrate (L-Phe), an inhibited state with ... conformational changes at the active site [21] are propagated to the rest of the full-length protomer in the oligomeric forms The structural changes observed at the active site of the catalytic ... alone the [S]0.5-value for the cofactor was measured to 5.6 0.8 lM with a D R-value of 25 RU/(pmol subunitặmm)2) at saturation [26] Effect of active site ligands on the rate of phosphorylation of...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
... interactions that hold the terminal glucose of the bglucan substrate in the )1 subsite at the bottom of the active site pocket [20] The entrance to the active site pocket of Exg is flanked by a pair of phenylalanyl ... indicated by the black bars and transglucosylation activities by grey bars Note that the double mutant was inactive in both assays Production of enzyme variants Recombinant native Exg and the ... may be relevant The relative activities of the mutants tested showed the same trend for both the hydrolysis and transglycosylation assays The observation that the transglycosylation assay appeared...
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Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
... generated using CLUSTALW The most likely candidate for the catalytic glutamate of propionate CoAtransferase based on these data was glutamate 324 (Fig 3) Detection of glutamate 324 as the catalytic ... the catalytic carboxylate of propionate CoA-transferase As the sequence analysis did not allow an unequivocal identi®cation of the catalytic glutamate of propionate CoAtransferase, this residue ... 30% of the total protein when the enzyme was incubated with 100 lM propionyl-CoA, prior to sample preparation These data indicated that a small but signi®cant fraction of the puri®ed CoA-transferase...
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Báo cáo Y học: Biosynthesis of vitamin B2 An essential zinc ion at the catalytic site of GTP cyclohydrolase II ppt
... Table can catalyze the formation of GMP from GTP, albeit at a reduced velocity Specifically, the rate for the C54S mutant was 60% of that of the wild-type, and the relative rates of the C65S and ... investigated the type II enzyme for the presence of zinc The data are consistent with the presence of one zinc ion per subunit of the homodimeric enzyme of E coli The comparison of numerous putative ... hydrolysis of the formamide-type product with formation of formate These findings suggest the hypothetical mechanism shown in Fig The formation of a covalent linkage between the substrate and the enzyme...
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Báo cáo Y học: Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell ppt
... inactivated to the extent of about 60% with mM PP in 15 The rate of inactivation of the EAC enzyme was a function of the reagent concentration although at any Fig Inactivation of Gra3P DH by PP The ... value of 9.0 due to rapid loss of the enzyme activity of the control sample It was observed that for the EAC cell enzyme, the rate of inactivation was increased with the increase in pH of the incubation ... phosphate buffer, pH 8.0 with indicated concentrations of TNBS or PP After 30 of incubation, the enzyme activity in an aliquot of the incubation mixture was measured, which indicated that the enzyme...
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Báo cáo khoa học: Aromatic amino-acid residues at the active and peripheral anionic sites control the binding of E2020 (AriceptÒ) to cholinesterases doc
... interacting only at the peripheral anionic site of the mutant enzyme As the active- site gorge of BChE is larger than that of AChE, and the distance between the indanone and the phenyl ring of E2020 is ... shorter than the distance between the active- site and the peripheral anionic site, E2020 can either bind at the active site or at the peripheral anionic site The observed dependence of the inhibitory ... interacts with Trp84 at the bottom of the gorge, the piperidine ring interacts with Tyr70, Asp72, Tyr121 and Tyr334 in the middle of the gorge, and the indanone ring interacts with Trp279 at the lip of...
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Báo cáo lâm nghiệp: "Measurement of free shrinkage at the tissue level using an optical microscope with an immersion objective: results obtained for Douglas fir (Pseudotsuga menziesii) and spruce (Picea abies)" potx
... specimens, in spite of their very small size, the good control of the saturated state and the mathematical approach used to measure the shrinkage values are certainly among the key features of our method, ... the same experimental protocol, the “initial” image being that of the test pattern in water with the immersion objective, and the “deformed” image being that of the test pattern taken using the ... case the datum of interest If two points on the reference configuration (saturated state) are called A0 and A, and the position of these two material points identified on the generic configuration...
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báo cáo khoa học: "An attempt to modify allelic frequencies at the Adh locus of a Drosophila melanogaster population in a tropical environment" pot
... were attracted by banana baits in buildings ; among these, D melanogaster adults represented more than 95 p 100 of the total catch, indicating that adults of this species are attracted by human ... modification was observed ; the significance of the data has been discussed II Methods The experiment was done on the campus of the University of Calavi near Cotonou A previous study had shown that ... considered as a kind of ecological island for D melanogaster populations (R.P Benin) Prior to the experiment, banana baits were put in the building of the department of Zoology and flies were regularly...
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Luận Án TS Y học: Patient satisfaction with health services at the outpatient department of Indira Gandhi memorial hospital, male’ Maldives.
... to the features of the setting in which the health services are provided This includes pleasantness of atmosphere, clear sign and direction, enough toilet and clean drinking water The answer of ... problem; Mean, median standard deviation, maximum and minimum were calculated for quantitative data’s Quartile deviations, maximum and minimum were calculated for patient satisfaction, attitude and ... tidiness of the OPD atmosphere The patients were most felt (15.1%) very satisfied with the good ventilation inside the hospital The median, quartile deviation, maximum and minimum score were calculated...
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An investigation into the attitudes of teachers and students at the hanoi college of industrial economics towards speaki
... so that the bad can keep up with the others Give them more time for preparation and respect their extra- preparation and practice Establish close and good relationship with the learners and ... of the students want to enhance listening and speaking skills on one hand and develop their English for Economics on the other hand with the hope that they can use English as a useful tool in their ... contents of the topics, the activities and the time of the lessons in the class 18% of the respondents said that the topics were quite suitable but 17% of respondents answered that they were...
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Báo cáo y học: "Effects of Losartan on expression of connexins at the early stage of atherosclerosis in rabbits"
... and endothelium and resulted in the formation of atherosclerosis (9) Javid et al also found that in the early stage of atherosclerosis, the number of Cx43 gap junction plaques increased and the ... connections The present study aimed to detect the expression of Cx40 and Cx43 in the artery at early stage of high fat diet induced atherosclerosis and to investigate the effects of AT1 antagonist ... significant difference was observed between the Losartan group and the high fat diet group (P>0.05) The body weight at the end of experiment was did not change significantly compared with that at...
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INTRODUCTION - The Hidden Side of Everything
... However, they can be beat at their own game And in the face of the Internet, their informational advantage is shrinking every day—as evidenced by, among other things, the falling price of coffins and ... appealing and the other not so The appealing candidate raises much more money and wins easily But was it the money that won him the votes, or was it his appeal that won the votes and the money? That’s ... lot of questions, some frivolous and some about life-and-death issues The answers may often seem odd but, after the fact, also rather obvious We will seek out these answers in the data—whether...
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Meeting the Business Side of Google
... its participants succeeded), and efficiency (participants controlled their costs and tracked their returns) In time, the advantages of eBay’s system got the attention of midsize brick-and-mortar ... publishers, manufacturers, and e-tailers As with eBay, small and large participants enjoy the same benefits The Three Goals of Every Webmaster Innumerable business plans operate side by side on the Web ... primary relationship is between Google and the consumer Without satisfied searchers, the business side has no value Consumers may freely focus on the search experience, with no awareness of the business...
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LECTURE SLIDES ON NONLINEAR PROGRAMMING BASED ON LECTURES GIVEN AT THE MASSACHUSETTS INSTITUTE OF TECHNOLOGY CAMBRIDGE, MASS DIMITRI P. BERTSEKAS
... Minima • The Role of Convexity MATHEMATICAL BACKGROUND • Vectors and matrices in n • Transpose, inner product, norm • Eigenvalues of symmetric matrices • Positive definite and semidefinite matrices ... Quadratic Approximation of f at x1 Slow convergence of steepest descent Fast convergence of Newton’s method w/ αk = Given xk , the method obtains xk+1 as the minimum of a quadratic approximation of ... PROGRAMMING LECTURE 5: RATE OF CONVERGENCE LECTURE OUTLINE • Approaches for Rate of Convergence Analysis • The Local Analysis Method • Quadratic Model Analysis • The Role of the Condition Number...
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Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf
... the rate of cleavage of both types of substrates The effect of the G372(223)A mutation on Km is more conspicuous with peptidyl substrates than with FX, indicating that substrate subsites located ... methoxypolyethyleneglycol-butyraldehyde with an average MW of 2000 ) which allows visualization of the modication using SDSPAGE The rate of carbamylation of the N-terminal amino group [Ile153(16)] of the protease domain ... comparisons of the two forms of FVIIa were performed using the concentrations of active enzyme The enzymes were diluted to a concentration of 100 nm based on the measured absorbance of the stock...
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Tài liệu The Proof is in the Pudding - A Look at the Changing Nature of Mathematical Proof doc
... nevertheless the case that proof is the lingua franca of mathematics It is the web that holds the enterprise together It is what makes the subject travel well, and guarantees that mathematical ... bullet-proof fashion that will stand the test of time The second, and for the working mathematician the most important, aspect of proof is that the proof of a new theorem explains why the result ... consist of an agglomeration of these various techniques One of the main purposes of the present book is to present and examine all these many forms of mathematical proof, and the role that they...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose