Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase pptx

Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase Diana Zhiryakova 1 , Ivaylo Ivanov 2 , Sonya Ilieva 3 , Maya Guncheva 1 , Boris Galunsky 4 and Nicolina Stambolieva 1 1 Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria 2 Faculty of Biology, University of Sofia ‘Sv. Kl. Ohridski’, Bulgaria 3 Faculty of Chemistry, University of Sofia ‘Sv. Kl. Ohridski’, Bulgaria 4 Institute of Technical Biocatalysis, Hamburg University of Technology, Germany The N-terminal nucleophile (Ntn) hydrolase superfam- ily comprises enzymes sharing a characteristic organi- zation of the secondary structure in the catalytic domain, despite the very low sequence homology [1,2]. The reaction mechanism that is suggested to be com- mon for all Ntn hydrolases resembles that of serine proteases, involving consecutive enzyme acylation and deacylation steps. A feature of the catalytic mechanism Keywords catalytic mechanism; Hammett plot; N-terminal nucleophile (Ntn) hydrolase; penicillin G acylase; quantum mechanical (QM) and molecular mechanical (MM) modeling Correspondence D. Zhiryakova, Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, 9 Acad. G. Bonchev str., 1113 Sofia, Bulgaria Fax: +359 2 870 0225 Tel: +359 2 9606 160 E-mail: diana_zh@yahoo.com (Received 25 September 2008, revised 28 January 2009, accepted 27 February 2009) doi:10.1111/j.1742-4658.2009.06987.x A new set of experimental kinetic data on the hydrolysis of a series of phenylacetyl p-substituted anilides catalyzed by penicillin G acylase from Escherichia coli (PGA) is presented in this article. The Hammett plot of log(k cat,R ⁄ k cat,H ) versus r p ) has three linear segments, which distinguishes the enzyme from the other N-terminal nucleophile hydrolases for which data are available. Three amino acids in the vicinity of the catalytic SerB1 (AsnB241, AlaB69, and GlnB23) were included in the quantum mechanical model. The stable structures and the transition states for acylation were optimized by molecular mechanical modeling and at the AM1 level of the- ory for three model substrates (with H, a methoxy group or a nitro group in the para position in the leaving group). Intrinsic interactions of several functional groups at the active site of PGA are discussed in relation to the catalytic efficiency of the enzyme. The energy barrier computed for the first step of acylation (the nucleophilic attack of SerB1) is lower than that for the second step (the collapse of the tetrahedral intermediate). However, the electronic properties of the substituent on the leaving group affect the structure of the second transition state. It is shown that the main chain car- bonyl group of GlnB23 forms a hydrogen bond with the leaving group nitrogen, thus influencing the hydrolysis rate. On the basis of our computa- tions, we propose an interpretation of the complex character of the Ham- mett plot for the reaction catalyzed by PGA. We suggest a modified scheme of the catalytic mechanism in which some of the intramolecular interactions essential for catalysis are included. Abbreviations AE, acyl enzyme; AGA, aspartylglucosaminidase; ES, enzyme–substrate; GGT, c-glutamyl transpeptidase; MM, molecular mechanical; Ntn, N-terminal nucleophile; PGA, penicillin G acylase from Escherichia coli; QM, quantum mechanical; TI, tetrahedral intermediate; TS, transition state. FEBS Journal 276 (2009) 2589–2598 ª 2009 The Authors Journal compilation ª 2009 FEBS 2589 of Ntn hydrolases is that the nucleophile (side chain hydroxyl or thiol group), which attacks the carbonyl carbon of the scissile amide bond, and the general base, which accepts the proton from the nucleophile, belong to the same N-terminal amino acid residue (Ser, Thr, or Cys). The N-terminal nucleophile is engaged in a hydrogen bond network, which has stabi- lizing and activating functions in addition to maintain- ing the proper spatial structure of the active site [3–5]. Therefore, the catalytic efficiency of Ntn hydrolases strongly depends on intrinsic interactions within the reaction intermediates. A deeper understanding of the catalytic mechanism requires knowledge of the contri- butions of the supporting, ‘catalytically insignificant’ amino acids to the energetics of the reaction. The present article is focused on the hydrolytic reac- tion catalyzed by penicillin G acylase from Escherichia coli (PGA; EC 3.5.1.11). PGA is widely used for hydrolytic and synthetic transformations in laboratory- scale organic synthesis and in the industrial production of semisynthetic b-lactam antibiotics [6–9]. The enzyme is one member of the Ntn superfamily, which is struc- turally very well characterized. Much information has been accumulated from crystallographic and kinetic studies, as well as from site-directed mutagenesis [10– 14]. The mature PGA is a periplasmic 86 kDa hetero- dimer of A and B chains (209 and 557 amino acids, respectively). The side chain hydroxyl of the N-termi- nal SerB1 was identified as the attacking nucleophile. Its specificity is determined mainly by the acyl moiety of the substrate: phenylacetyl derivatives have the low- est K m values. In contrast, the leaving group can vary from ammonia to 6-aminopenicillanate through a wide range of compounds with a primary amino or hydroxyl group. Here we present a new set of kinetic data on the PGA-catalyzed hydrolysis of a series of phenylacetyl p-substituted anilides (Scheme 1). For the interpreta- tion of the experimental results, a quantum mechanical (QM) model of the enzyme active site was constructed. It illustrates the reorganization of the hydrogen bond network at the active site, which predetermines the cat- alytic transformations. Several functional groups in the proximity of SerB1 were assigned probable roles in catalysis. Results and Discussion The results of the kinetic experiments are presented in Table 1. All substrates have Michaelis constants of the same order of magnitude. This confirms an earlier con- clusion, that the p -substituent on the leaving group influences the reaction rate by its electronic properties and does not affect substrate binding (Fig. S1) [15]. On the other hand, the hydrolysis rate varies with the substituent on the amino moiety of the substrate, con- firming that the formation of acyl enzyme (AE) is the rate-limiting step of the reaction. The Hammett plot of log(k cat,R ⁄ k cat,H ) versus r p ) is shown in Fig. 1. No cor- relation was observed between the rate constant and the van der Waals volume, the Taft steric parameter, or the hydrophobic parameter of the substituent R. The Brønsted plots of log( k cat,R ⁄ k cat,H ) versus pK a,1 and pK a,2 of the leaving aniline are given in Fig. S2. The Hammett plot can be divided into three linear segments: the points corresponding to substrates with electron-donating substituents (R = CH 3 or OCH 3 ) lie on a line with a negative slope q = ) 2.95 ± 0.98; for substrates with moderate electron-withdrawing substit- uents (R = Br, CF 3 , COOC 2 H 5 or COCH 3 ) the slope is positive q = 0.90 ± 0.10; surprisingly, substrates with R = CN or NO 2 lie on a line with a negative slope q = )0.50 ± 0.18. The dependence of the rate on the electronic factor of the substituent for the PGA-catalyzed hydrolytic reaction is very distinct as compared with the other Ntn hydrolases for which data are available [16,17]. The Hammett plot of the transpeptidation reaction catalyzed by c-glutamyl transpeptidase (GGT; EC 2.3.2.2), is biphasic, displaying a negative slope for the electron-donating substituents (q = )1.3) and a positive slope for the electron-withdrawing substituents (q = 0.4). The gly- cosylasparaginase-catalyzed hydrolysis [aspartylgluco- saminidase (AGA), EC 3.5.1.26] is also characterized by a biphasic dependence: substrates with electron- donating groups give a line with slope q = )0.94, and substrates with electron-withdrawing groups give a line with slope q = 0.70. For all three enzymatic reactions (GGT-catalyzed transpeptidation, and AGA-catalyzed and PGA-catalyzed hydrolysis), acylation is the rate- limiting step [16–18]. However, the values of q for Scheme 1. PGA-catalyzed hydrolysis of a series of phenylacetanilides. Supporting amino acid residues at PGA active site D. Zhiryakova et al. 2590 FEBS Journal 276 (2009) 2589–2598 ª 2009 The Authors Journal compilation ª 2009 FEBS PGA are higher than those reported for glycosylaspar- aginase and GGT, indicating that the PGA-catalyzed hydrolysis is much more sensitive to the electronic properties of the substituent on the leaving group. The third segment, corresponding to strong acceptor groups on the leaving aniline of the substrate, repre- sents a substantial difference and a key feature of the PGA catalytic reaction. It is probably a cumulative result of intrinsic amino acid interactions in the active site of the E. coli enzyme that differentially (de)stabi- lize the structures along the reaction pathway. The interpretation of the presented kinetic results requires a more sophisticated QM model that would most adequately and yet economically reflect the inter- actions at the PGA active site during catalysis. Three residues in the vicinity of the N-terminal nucleophile were selected for the construction of the model: AsnB241, AlaB69, and GlnB23. AsnB241 proved to be indispensable for catalysis, as its mutation to Ala led to a dramatic reduction of the catalytic activity, with a minor effect on proenzyme processing [11]. AsnB241, together with the main chain amide of AlaB69, forms the oxyanion hole that balances the negative charge and thus lowers the energy of the reactive tetrahedral intermediate (TI). In addition, it contributes to correct positioning of the substrate against the catalytic SerB1. GlnB23 was also shown to interact with the nucleo- phile and the general base, and contribute to the stabilization of the TI [5,10]. Table 2 presents data on the interatomic distances in the crystal structures of the wild-type enzyme, its complex with a competitive Table 1. Kinetic parameters of the PGA-catalyzed hydrolysis of phenylacetyl p-substituted anilides. Values of r p ) are from [31]. pK a,1 refers to the equilibrium H 3 N + Ar ¡ H 2 NAr + H + ;pK a,2 refers to the second dissociation step, H 2 NAr ¡ ) HNAr + H + . PhCH 2 CONHC 6 H 4 -pR r p ) pK a,1 pK a,2 k cat (s )1 ) K m (mM) k cat ⁄ K m (mM )1 Æs )1 )Substituent R Name of substrate NO 2 N-(4-Nitrophenyl)-2-phenylacetamide 1.27 1.02 18.9 16.7 ± 0.5 0.16 ± 0.01 104.4 CN N-(4-Cyanophenyl)-2-phenylacetamide 1.00 1.74 22.7 23.3 ± 1.1 0.52 ± 0.04 44.8 COCH 3 N-(4-Acetylphenyl)-2-phenylacetamide 0.84 2.19 22.6 a 27.4 ± 0.8 0.20 ± 0.01 137.0 COOC 2 H 5 Ethyl 4-[(phenylacetyl)amino]benzoate 0.75 2.38 21.6 ± 1.0 0.10 ± 0.01 216.0 CF 3 2-Phenyl-N-[4-(trifluoro-methyl)-phenyl]acetamide 0.65 2.57 24.3 a 17.7 ± 1.6 0.35 ± 0.09 50.6 Br N-(4-Bromophenyl)-2-phenylacetamide 0.25 3.88 26.0 a 7.5 ± 0.9 0.23 ± 0.04 32.6 H N,2-Diphenylacetamide 0.00 4.58 27.0 4.8 ± 0.4 0.14 ± 0.02 34.3 CH 3 N-(4-Methylphenyl)-2-phenylacetamide )0.17 5.07 5.1 ± 0.2 0.11 ± 0.01 46.4 OCH 3 N-(4-Methoxyphenyl)-2-phenylacetamide )0.26 5.30 9.4 ± 0.7 0.10 ± 0.02 94.0 a For these substrates, pK a2 values in water were not available. They were calculated from those for dimethylsulfoxide, using a linear correla- tion [32]. –0.4 –0.2 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 –0.2 0.0 0.2 0.4 0.6 0.8 1 . 0 lg(k cat, R /k cat, H ) O NH R –NO 2 –CN –COCH 3 –COO 2 CH 5 –CF 3 –Br –H –OCH 3 –CH 3 σ P – Fig. 1. Hammett plot for the hydrolysis of a series of phenylacetyl p-substituted anilides catalyzed by PGA. The insert shows the general formula of the substrates. Table 2. Interatomic distances in the crystal structures of wild-type PGA and the complexes with phenylacetic acid, penicillin G sulfoxide (PGSO), and its phenylmethanesulfonyl-serylB1 derivative. Distance (A ˚ ) Ligand Protein Data Bank ID Reference NaGlnB23 –OcSerB1 OeGlnB23 –NaSerB1 OdAsnB241 –NaSerB1 OaGlnB23 –ligand 2.89 3.18 3.01 1pnk [10] 2.89 3.18 3.01 3.86 (O ⁄ COO ) ) PhCH 2 COOH 1pnl [10] 2.69 2.97 2.89 3.60 (N ⁄ NH) PGSO 1gm9 [11] 2.68 3.26 2.86 3.39 (O ⁄ SO 2 ) PhSO 2 -Oc SerB1 1pnm [10] D. Zhiryakova et al. Supporting amino acid residues at PGA active site FEBS Journal 276 (2009) 2589–2598 ª 2009 The Authors Journal compilation ª 2009 FEBS 2591 inhibitor, the complex with a slowly hydrolyzed sub- strate, and the phenylmethanesulfonyl-SerB1 deriva- tive. These structures are mimics of the stationary points along the reaction pathway; they depict the changes in the spatial structure of the active site during catalysis. Phenylacetic acid induces no essential change in the conformation of the enzyme. Substrate binding causes considerable shortening of the hydrogen bonds of the side chain hydroxyl of SerB1 with the backbone amino group of GlnB23 and with the side chain car- bonyl group of AsnB241. The main chain carbonyl group of GlnB23 closely interacts with the oxygen atom in the locality of the nucleofuge in the structural analog of the TI. These experimentally obtained data form the basis for the construction of our model, which is described in detail in Experimental procedures. The first stage of the catalytic cycle, enzyme acyla- tion, proceeds via two steps separated by a relatively stable intermediate, the TI. The structures of the enzyme–substrate (ES) complex, AE with free aniline, the TI, and two transition states (TSs) were optimized for the model substrate CH 3 CONHC 6 H 5 in the envi- ronment of the catalytic and the supporting amino acids. Table 3 presents the geometry parameters, which undergo significant changes during catalysis, optimized by AM1 and HF ⁄ 6-31G** QM computations and molecular mechanical (MM) modeling with the Dreid- ing force field. The initial Michaelis complex (ES) fea- tures spatial approximation of the side chain of SerB1 to the carbonyl carbon of the substrate, which is favor- able for nucleophilic attack (Table 3 and Fig. S3). The Michaelis complex of penicillin G with PGA has a similar structure, and is shown in Fig. S4. In the TS (TS1), which separates the ES complex from the TI, the proton transfer from the nucleophile to the general base is practically completed, and the bond between Oc of SerB1 and the carbonyl carbon of the substrate is partially formed. The positive charge of the a-amino group of SerB1 in TS1 is stabilized by three hydrogen bonds: two with the side chain carbonyl oxygens of GlnB23 and AsnB241, and a bifurcate bond with its own side chain oxygen and the carbonyl oxygen of the substrate. The hydrogen bond between the negatively charged Oc SerB1 and the main-chain NH of GlnB23 becomes stronger upon the nucleophilic attack. The energy of the TI for acetanilide, estimated by AM1 computations, is 29.72 kcalÆmol )1 relative to the energy of the ES complex. Its optimized structure is shown in Fig. 2. The interactions with the oxyanion-hole resi- dues AsnB241 and AlaB69 strongly decrease the energy of the TI. As our model shows, the oxyanion is also hydrogen-bonded to the positively charged a-amino group of SerB1, thus gaining additional sta- bilization. The a-amino group of the aspartyl moiety in b-N-acetylglucosaminyl-l-asparagine has a similar function in the AGA-catalyzed hydrolysis [3]. Proba- bly, such additional stabilization by a protonated amino group in the proximity of the oxyanion can be found in the rest of the Ntn hydrolases [3]. From another point of view, the hydrogen bond between the oxyanion and the aNH 3 + of SerB1 resembles the one between the carboxylate group of the Asp residue and the protonated imidazole ring of the His residue in the serine protease catalytic triad. In the second TS (TS2) of the expulsion of the p-substituted aniline, the bond between the oxyanion and the protonated a-amino group of SerB1 is weaker than in TS1, allowing for Table 3. Selected interatomic distances in the stationary points of the modeled PGA-catalyzed hydrolysis of acetanilide (R = H) and p-nitro- acetanilide (R = NO 2 ) optimized by AM1 calculations. Atoms a Distance (A ˚ ) ES R=H TS1 R=H TI R=H TI b R=H TI c R=H TS2 R=H TS2 R=NO 2 AE + H 2 NC 6 H 5 R=H N M C 1.38 1.43 1.50 1.47 1.45 1.57 (1.57) 1.95 (1.92) 2.87 Oc SerB1 M C 3.37 1.84 1.50 1.49 1.51 1.48 (1.47) 1.42 (1.42) 1.37 N M H(Na SerB1 ) 3.52 3.04 2.60 2.95 2.23 1.36 (1.38) 1.71 (1.60) 1.00 N M Na SerB1 3.62 4.05 3.06 3.74 2.89 2.62 (2.61) 2.76 (2.68) 3.24 Oc SerB1 M Na GlnB23 3.04 2.92 3.01 3.1 2.68 3.02 3.07 3.12 Na SerB1 M Oe GlnB23 4.36 2.92 2.85 2.88 3.00 3.00 2.90 3.04 Na SerB1 M Od AsnB241 3.01 2.78 2.73 2.79 2.74 2.98 (2.86) 2.81 (2.78) 3.20 Na SerB1 M O 3.05 2.81 2.80 2.65 2.78 3.22 (2.94) 2.91 (2.85) 4.44 N M O(C=O) GlnB23 3.02 3.21 3.24 3.28 2.59 3.20 3.23 3.21 Na SerB1 M Oc SerB1 2.97 2.80 2.94 2.85 2.86 2.97 (2.96) 2.97 (2.97) 2.94 a If not designated otherwise, atoms belong to the former substrate. b From HF ⁄ 6-31G** QM calculations. c From MM calculations with the Dreiding force field. Optimized geometry parameters without GlnB23 in the model are given in parentheses. Supporting amino acid residues at PGA active site D. Zhiryakova et al. 2592 FEBS Journal 276 (2009) 2589–2598 ª 2009 The Authors Journal compilation ª 2009 FEBS restoration of the carbonyl function. The hydrogen bonds of a-NH 3 + of SerB1 with OeGlnB23 and OdAsnB241 are elongated. The protonated general base is situated closer to the leaving group nitrogen atom, and the proton transfer for the expulsion of the aniline is in progress (general acid catalysis). The untypical Hammett dependence observed for the PGA-catalyzed hydrolysis of phenylacetyl anilides indicates that a change in the reaction pathway or the rate-limiting step occurs depending on the structure of the substrate. For the model substrate acetanilide, the energy barrier computed for the first step (the nucleo- philic attack of SerB1), is 32.34 kcalÆmol )1 , relative to the energy of the ES complex. For the second step, the barrier is 37.00 kcalÆmol )1 , and this identifies the col- lapse of the TI as the rate-limiting step of acylation. This is also true for the nitro-substituted and methoxy- substituted acetanilides, as well as for a different type of substrate – N-methylacetamide. However, in the lat- ter case, the two energy barriers are almost leveled, the difference being only 2 kcalÆmol )1 . The conclusions of Menard et al. on the transpeptidation reaction cata- lyzed by GGT are similar [16], as are the calculations of Galabov et al. concerning the energetics of the alka- line hydrolysis of N-phenylacetamides [19]. The investi- gation of Perakyla et al. on the catalytic reaction of AGA, however, predicts that, energetically, the highest point in the acylation step is the nucleophilic attack on the carbonyl carbon of the substrate [3]. Our result disagrees with the conclusion of Chilov et al., who employed a different model system [20]. The two mod- els are in good agreement on the geometry of the sta- tionary points on the potential energy surface of the PGA-catalyzed hydrolysis. However, they disagree on the energetics of the reaction, most probably because of the substitution of the oxyanion hole with two water molecules. The effective stabilization of the oxy- anion is the main driving force of the first step of acyl- ation, and dominates all other electronic effects on the reaction center. The model system presented here more closely reflects the structure and electron density distri- bution of the real substrates and PGA active site. In order to interpret the biphasic character of the Hammett plots available for AGA and GGT, it was suggested that the breakdown of the tetrahedral struc- ture proceeded via general acid catalysis, whereby the proton transfer to the nitrogen of the leaving group aniline occurs simultaneously with the C–N bond cleavage [17]. Accordingly, the degree of proton trans- fer and C–N bond cleavage would both depend on the electronic nature of the p-substituent [16]. Figure 3 shows the optimized structures of the second TSs of the PGA-catalyzed hydrolysis of acetanilide (R = H) and p-nitroacetanilide (R = NO 2 ). As can be seen, the stabilizing interactions of the oxyanion with AsnB241, AlaB69 and the a-amino group of SerB1 become weaker in TS2; the carbonyl group of the substrate is partially restored, and the protonated general base is properly oriented to give a proton to the leaving group nitrogen (Table 2). In the case of R = H, the proton transfer is 40% complete, whereas the amidic C–N bond is cleaved to a very small degree. The TS resem- bles the structure denoted TS2a in Fig. 4. The partial positive charge on the nucleofuge nitrogen is stabilized by hydrogen bonding with the main chain carbonyl oxygen of GlnB23. Removing this residue from the QM model results in practically no change in the struc- ture of TS2. Energetically, its absence is partially com- pensated for by a stronger interaction of the aNH 3 + of SerB1 with the oxyanion and with the side chain of AB Fig. 2. Optimized structures of the TI of hydrolysis of acetanilide by QM calculations at the AM1 level (A) and of N,2-diphenyl- acetamide by Dreiding force field MM modeling (B). D. Zhiryakova et al. Supporting amino acid residues at PGA active site FEBS Journal 276 (2009) 2589–2598 ª 2009 The Authors Journal compilation ª 2009 FEBS 2593 AsnB241 (Table 3). The calculations show that TS2 for R = OMe does not differ substantially in structure from that of acetanilide. However, as can be seen from Fig. 4, GlnB23 makes a significant contribution to the protonation of the leaving group prior to its expulsion. The positive combination of the electronic factor of R and the hydrogen bond between the leaving group nitrogen atom and the main chain carbonyl oxygen of GlnB23 is evidenced by the steep increase of hydrolysis rate constant with the decrease of r p ) for the electron- donating substituents (R = CH 3 or OCH 3 ) (Fig. 1). The second TS of acylation for p-nitroacetanilide features a high degree of C–N bond cleavage and a very low degree of protonation of the leaving group (Fig. 4). The aromatic system provides resonance stabilization of the partial negative charge on the nitrogen. Electron-withdrawing substituents further decrease the energy of the stationary point. Starting from R = H with the increase in the r p ) value, TS2 shifts in the direction of TS2b, and the expulsion of an anilide anion is more favored. The main chain car- bonyl group of GlnB23 can still be hydrogen-bonded to the leaving group nitrogen (Fig. 3). In the cases of R = Br, CF 3 , COOC 2 H 5 or COCH 3 , the resultant effect is a slow increase of the hydrolysis rate constant with an increase in r p ) , i.e. a small value of q.In comparison with the unsubstituted substrate, the absence of GlnB23 in the QM model has a greater AB Fig. 3. Optimized structures of the second TS of hydrolysis of acetanilides with (A) R = H and (B) R = NO 2 in the para position in the leaving group. The arrows on the TS structures indicate the reaction coordinate with an imaginary frequency. N–H formation C–N cleavage CH 3 N H 3 + O H NB n R O – TI NH 2 R CH 3 N H 2 O Bn O H 2 N O H N + Bn R O – H TS2a CH 3 N H 3 + O Bn O H N – R TS2b AE Fig. 4. More O’Ferrall diagram for the brea- kdown of the TI of the PGA-catalyzed hydro- lysis of CH 3 CONHC 6 H 4 -pR with GlnB23 (circles) and without GlnB23 (triangles) in the constructed model. Solid symbols, R = H; gray symbols, R = OMe; open symbols, R = NO 2 . Supporting amino acid residues at PGA active site D. Zhiryakova et al. 2594 FEBS Journal 276 (2009) 2589–2598 ª 2009 The Authors Journal compilation ª 2009 FEBS effect on the structure of TS2 for p-nitroacetanilide. The nucleofuge is protonated to a higher degree, and the amidic C–N bond is a little shorter; the a-amino group of SerB1 interacts closely with the oxyanion (Fig. 3). This indicates that GlnB23 promotes the for- mation of an anilide ion. Probably, the combination of stronger resonance stabilization by the strong electron- withdrawing groups (R = CN and NO 2 ) and the hydrogen bonding between the leaving group nitrogen and the main chain carbonyl of GlnB23 leads to the formation of a TS similar to TS2b. The subsequent protonation of the nucleofuge becomes the rate-limit- ing step of enzyme acylation. This is shown by the negative slope of the third segment of the Hammett plot for the strong electron acceptors. The small difference between the hydrolysis rates of esters and amides of common acyl moieties is evi- dence for the effect of GlnB23 at the active site of PGA on the energetics of the reaction. The values of k cat for methyl 2-phenylacetate and 2-phenylacetamide are 190 and 50 s )1 , respectively. For both substrates, acylation is rate-limiting, as the rate constant of deac- ylation is over 1000 s )1 [18]. For comparison, AGA hydrolyzes the b-methyl ester of Asp faster than the amide (Asn), the rate constants differing by several orders of magnitude [21]. The leaving alcohol ⁄ alcox- ide group cannot form a hydrogen bond with the main chain carbonyl of GlnB23. Most probably, the repulsion between the two oxygen atoms destabilizes the second TS and leads to decreased catalytic effi- ciency in ester hydrolysis. TyrB444 at the active site of GGT (Protein Data Bank ID: 2dbx [22]) can inter- act with the leaving group similarly to the main chain carbonyl group of GlnB23 in PGA. However, this Tyr residue can be both a donor and an acceptor of a hydrogen bond. Such an interaction cannot be real- ized at the active site of AGA, because no proper functional group could be found within a 4 A ˚ radius from the leaving group heteroatom (Protein Data Bank ID: 1apz [23]). GlnB23 plays critical role in the process of deacyla- tion. The acyl acceptor (a water molecule) forms a hydrogen bond with the main chain carbonyl oxygen of GlnB23 [12]. This bond is present in the intermedi- ate formed after the nucleophilic attack of the water molecule. Its deprotonation by the a-amino group of SerB1 is favored by stronger interaction of the general base with the side chain oxygens of AsnB241 and Scheme 2. Modified scheme of the cata- lytic mechanism of the PGA-catalyzed hydrolytic reaction. D. Zhiryakova et al. Supporting amino acid residues at PGA active site FEBS Journal 276 (2009) 2589–2598 ª 2009 The Authors Journal compilation ª 2009 FEBS 2595 GlnB23 (the basicity of aN of SerB1 is increased). Thus TI deac. is formed, in which the oxyanion is stabi- lized by the oxyanion hole residues and additionally by the protonated a-amino group of SerB1. During the breakdown of TI deacyl. , the increasing negative charge of the leaving OcSerB1 is balanced by the main chain NH of GlnB23. On the basis of the presented kinetic results and the QM model, we propose a modified scheme of the cata- lytic mechanism of PGA, in which some of the intra- molecular interactions essential for catalysis are included (Scheme 2). Conclusion We present an experimental Hammett plot for the PGA-catalyzed hydrolysis of a series of phenylacetyl p-substituted anilides. The proposed interpretation of its complex character is based on an extended QM model, in which specific ES interactions are taken into account. Several functional groups in the vicinity of the catalytic center are assigned functions in catal- ysis. The a-amino group of SerB1 and the main chain NH of GlnB23 activate and stabilize the c-hydroxyl group of SerB1 for nucleophilic attack on the sub- strate. The protonated general base interacts with the side chain carbonyl oxygens of GlnB23 and AsnB241, and contributes to the stabilization of the oxyanion in the TI. The main chain carbonyl group of GlnB23 forms a hydrogen bond with the leaving group nitro- gen, thus influencing the hydrolysis rate. The specific orientation and interaction of several amino acids at the active site of PGA, combined with the effect of the substituent on the geometry of the second TS, leads to a change in the reaction pathway and the rate-limiting step for the strong electron-withdrawing substituents. Experimental procedures Organic solvents and initial arylamines were purchased from Fluka (Darmstadt, Germany) and used without further purification. PGA was purchased from Sigma (St Louis, MO, USA). PGA active site titration Aliquots of the enzyme solution were incubated for a set time with varying amounts of phenylmethanesulfonyl fluo- ride (irreversible inhibitor of serine proteases) in 50 mm sodium phosphate buffer (pH 7.0). An aliquot of every sample was then used to catalyze the hydrolysis of a constant amount of the chromogenic substrate 2-nitro-5- phenylacetamidobenzoic acid at 25 °Cin50mm sodium phosphate buffer (pH 7.0) containing 10% dimethylsulfox- ide (v ⁄ v). The release of 5-amino-2-nitrobenzoic acid allows the progress of the reaction to be followed spectro- photometrically (k = 380 nm). The decrease of PGA activity as a function of the amount of the inhibitor was used to calculate the molar concentration of the enzyme [24]. Synthesis of substrates for PGA N-(4-methoxyphenyl)-2-phenylacetamide, N-(4-methylphenyl)- 2-phenylacetamide, ethyl 4-[(phenylacetyl)amino]benzoate, N-(4-acetylphenyl)-2-phenylacetamide, N-(4-cyanophenyl)-2- phenylacetamide and N-(4-nitrophenyl)-2-phenylacetamide were obtained by the following procedure. One equivalent of the p-substituted aniline and 1.1 equivalent amino base (NEt 3 , N-methylmorpholine) were mixed in organic solvent (tetrahydrofuran, chloroform) at 0 °C, and 1.2 equivalents of phenylacetyl chloride were added dropwise. The mixture was stirred at room temperature for 1–5 h. At the end of the reaction, the hydrochloride of the organic base was fil- tered. When chloroform was used, the reaction mixture was washed consecutively with 0.1 m HCl, a saturated solution of NaHCO 3 , and distilled water, and dried over MgSO 4 . The organic solvent was evaporated, and the residue was crystallized from ethanol, except for N-(4-cyanophenyl)-2- phenylacetamide, which was crystallized from water, and N-(4-nitrophenyl)-2-phenylacetamide, which was crystallized from benzene. N,2-diphenylacetamide, N-(4-bromophenyl)- 2-phenylacetamide and 2-phenyl-N-[4-(trifluoromethyl)- phenyl]acetamide were obtained by Schotten–Baumann acylation: one equivalent of the p-substituted aniline was dissolved in 10% aqueous NaOH; ethanol was added to increase the solubility of the reagent. The mixture was cooled to 0 °C, and 1.2 equivalents of phenylacetyl chloride were added. The reaction mixture was stirred at room tem- perature for 1–5 h. The precipitate was filtered, washed with cold distilled water, and crystallized from EtOH (N,2- diphenylacetamide was crystallized from methanol ⁄ water). The purity of the synthesized substrates was confirmed by means of elemental analysis, 1 H-NMR spectroscopy (Bruker Avance DRX 250), and melting point determina- tion (Bu ¨ chi B-540). The results were in good agreement with those reported in the literature [15,25,26]. 2-Phenyl-N- [4-(trifluoromethyl)-phenyl]acetamide (melting point 161– 162 °C) was newly synthesized. The results of the elemental analysis were as follows for C 15 H 12 NOF 3 : calculated (%), C – 64.51, H – 4.33, and N – 5.02; found (%), C – 64.61, H – 4.38, and N – 4.97. Chemical shifts in the 1 H-NMR spectrum were as follows: d H , p.p.m. relative to tetramethylsilane (dimethylsulfoxide-D 6 ), 3.69 (2H, s, C 6 H 4 CH 2 CO), 7.34–7.22 (5H, m, C 6 H 4 CH 2 CO), 7.66 (2H, d, J = 8.5 Hz, NHC 6 H 4 CF 3 ), 7.81 (2H, d, J = 8.5 Hz, NHC 6 H 4 CF 3 ), and 10.54 (1H, s, CONH). Supporting amino acid residues at PGA active site D. Zhiryakova et al. 2596 FEBS Journal 276 (2009) 2589–2598 ª 2009 The Authors Journal compilation ª 2009 FEBS PGA-catalyzed hydrolysis of the phenylacetyl p-substituted anilides Reactions were performed at 25 °C in 0.1 m sodium phos- phate buffer (pH 7.0) containing 10% dimethylsulfoxide (v ⁄ v). The total volume of the reaction mixture was 2000 lL. The initial substrate concentration ranged from about 0.2 to 4.0 K m , with the exception of N-(4-cyanophenyl)-2-phenylacetamide, N-(4-bromophenyl)- 2-phenylacetamide, and 2-phenyl-N-[4-(trifluoromethyl)- phenyl]acetamide, in which cases the interval was narrower, owing to limited solubility of the substrates in the reaction mixture. The enzyme concentration was about three orders of magnitude lower. The reaction was followed using a Shimadzu UV-3000 UV–visible spectrophotometer at a wavelength with maximum difference between substrate and product molar absorption coefficients, as follows [R (PhCH 2 CONHC 6 H 4 -pR) – k detection (nm)]: NO 2 – 416; CN – 303; COCH 3 – 340; COOC 2 H 5 – 317; CF 3 – 260; Br – 266; H – 260; CH 3 – 265; and OCH 3 – 265. Calibra- tion curves for both the substrate and the free aniline were prepared, and initial velocities were calculated, taking into account both the consumption of the substrate and the lib- eration of the arylamine with the time. The kinetic experi- ments with each substrate concentration were performed in triplicate. The turnover number and the Michaelis constant were determined by nonlinear regression analysis. Computational methods The covalent complex of PGA with the irreversible inhibi- tor phenylmethanesulfonyl fluoride was taken as an initial structure (Protein Data Bank ID: 1pnm from the Research Collaboratory for Structural Bioinformatics Protein Data Bank, http://www.pdb.org). It was modified by the follow- ing procedure, using the ds viewerpro 6.0 software pack- age (Accelrys Software Inc., San Diego, CA, USA) (now Discovery Studio: http://accelrys.com/products/discovery- studio/). The sulfur in the SO 2 group was replaced with a carbon atom. One of the two oxygen atoms was trans- formed into O ) , and the other into nitrogen; thus, the two double S = O bonds were replaced by C–O ) and C–N bonds. The leaving group – a phenyl residue – was then added to the nitrogen atom. The resultant structure was the TI of the hydrolysis of phenylacetyl anilide (R = H). This enzymatic complex was then optimized by MM calculations with the Drieding force field, using ds viewer [27]. All atoms belonging to the protein moiety were fixed during the optimization, except for SerB1 covalently bound to the TI. The model of the active center of PGA was then con- structed using the optimized positions of the atoms of SerB1, AlaB69, AsnB241, GlnB23 and the TI after substitu- tion of the phenyl ring in the acyl moiety of the substrate with a hydrogen atom. The spatial structure of the model TI of acetanilide was optimized by AM1 QM computations with the gaussian 98 software package [28]. Based on the structure of TI for acetanilide, the two TSs of the nucleophilic attack of the side chain of SerB1 and the collapse of TI were modeled. The stable structures and the TSs were fully optimized at the AM1 level of theory [28]. All stationary points were further characterized by analytic computations of harmonic vibrational frequencies. TSs were located using the synchronous transit-guided quasi-Newton methods, implemented in gaussian [29]. 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Nat Struct Biol 2, 1102–1108. 24 Svedas VK, Margolin AL, Sherestyuk CF, Klyosov AA & Berezin IV (1977) Phenylmethanesulfonylfluoride inactivation of soluble and immobilized penicillin amidase from E. coli. Kinetic analysis and titration of active site. Bioorg Khim 3, 547–553. 25 Yamagami C, Takao N, Tanaka M, Horisaka K, Asada S & Fujita T (1984) A quantitative structure–activity study of anticonvulsant phenylacetanilides. Chem Pharm Bull 32, 5003–5009. 26 Birnbaum LS & Powell G (1945) Amines related to epi- nephrine. III. Amines of the eprocaine type. J Am Chem Soc 67, 1464–1466. 27 Mayo SL, Olafson BD & Goddard WA (1990) DREID- ING: a generic force field for molecular simulations. J Phys Chem 94, 8897–8909. 28 Frisch MJ, Trucks GW, Schlegel HB, Scuseria GE, Robb MA, Cheeseman JR, Zakrzewski VG, Montgom- ery JA, Stratmann RE, Burant JC et al. (1998) Gaussian 98 (Revision A.7). Gaussian, Inc., Pittsburgh, PA. 29 Peng C, Ayala PY, Schlegel HB & Frisch MJ (1996) Using redundant internal coordinates to optimize equi- librium geometries and transition states. J Comp Chem 17, 49–56. 30 Peng C & Schlegel HB (1994) Combining synchronous transit and quasi-newton methods for finding transition states. Israeli J Chem 33, 449–454. 31 Corwin H, Leo A & Taft RW (1991) A survey of Ham- met substituent constants and resonance and field parameters. Chem Rev 91, 165–195. 32 Koppel I, Koppel J, Maria P-C, Gal J-F, Notario R, Vlasov VM & Taft RW (1998) Comparison of Brønsted acidities of neutral NH-acids in gas phase, dimethyl sulfoxide and water. Int J Mass Spectrom Ion Processes 175, 61–69. Supporting information The following supplementary material is available: Fig. S1. Putative binding of phenylacetyl p-nitroanilide at the active site of penicillin G acylase. Fig. S2. Brønsted plots for the hydrolysis of a series of phenylacetyl p-substituted anilides catalyzed by penicillin G acylase from E. coli. Fig. S3. Michaelis complex of phenylacetanilide with penicillin G acylase optimized by molecular mechanics with the Dreiding force field. Fig. S4. Structure of the Michaelis complex of penicil- lin G with penicillin G acylase optimized by MM cal- culations with the Dreiding force field. This supplementary material can be found in the online version of this article. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supplementary materials supplied by the authors. Any queries (other than missing material) should be directed to the corre- sponding author for the article. Supporting amino acid residues at PGA active site D. Zhiryakova et al. 2598 FEBS Journal 276 (2009) 2589–2598 ª 2009 The Authors Journal compilation ª 2009 FEBS . Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G. electron-withdrawing groups give a line with slope q = 0.70. For all three enzymatic reactions (GGT-catalyzed transpeptidation, and AGA-catalyzed and PGA-catalyzed