Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase pptx

... charged a- amino group of SerB1, thus gaining additional sta- bilization. The a- amino group of the aspartyl moiety in b-N-acetylglucosaminyl-l-asparagine has a similar function in the AGA-catalyzed ... distri- bution of the real substrates and PGA active site. In order to interpret the biphasic character of the Hammett plots available for AGA and GGT, it was s...

Ngày tải lên: 16/03/2014, 01:20

... LA CHN PHNG PHP BO DNG Bấ TễNG HIU QU TRONG IU KIN KH HU NểNG M TS. H Ngc Khoa, ThS. Nguyn Hựng Cng Khoa Xõy dng Dõn dng v Cụng nghip, Trng i hc Xõy dng Bi bỏo trỡnh by kt qu nghiờn ... bng thc nghim mc nh hng ca phng phỏp bo dng bờ tụng ton khi, thi cụng trong iu kin khớ hu núng m Vit Nam n cỏc quỏ trỡnh vt lý (s bay hi nc v bin dng do) xy ra trong bờ tụng trong thi gian ......

Ngày tải lên: 07/03/2014, 03:20

... recruitment of the MRN complex, and full activation of ATM at the site of the damage, which also requires a functional c-MYC. Activation of ATM promotes phosphorylation of histone H2AX and activation of ... agents that may increase the mutation rate and promote the accumulation of genomic instability, thus altering the crucial biological processes such as the...

Ngày tải lên: 14/03/2014, 22:20

... 5¢-TG TAGTTTCATGGA TGCCACAG-3¢; Akt-1 forward, 5¢ -A ACGGACTTCGGGCTGTG-3¢; Akt-1 reverse, 5¢-TTGTC CTCCAGCACCTCAGG-3¢; CD36 forward, 5¢-TCCAGC CAATGCCTTTGC-3¢; CD36 reverse, 5¢-TGGAGATTAC A B EF G CD Fig. ... gene, was used as an internal control. The target mRNA was normalized against actin in the same sample. The PCR primers were as follows: actin forward, 5¢-GA AATCGTGCGTGACATCA...

Ngày tải lên: 16/02/2014, 09:20

Ngày tải lên: 16/02/2014, 15:20

... RNA cleavage, namely Arg67 and Arg71. Both arginines are found in the phosphate-binding site of RNase Sa2 and are con- served in all microbial RNases. The importance of Arg67 in RNA cleavage was ... on their equiv- alents in RNase Sa have shown that they are necessary for the catalytic reaction. At the pH optimum for the reaction, both arginines are protonated, facilitat...

Ngày tải lên: 18/02/2014, 11:20

... solvent accessibilities of the amino acid residues that are located around the mutation sites, including the parent and mutated residues at these sites, were calculated on the basis of their accessible ... reported. b Parameters characterizing the thermal denaturation of So-RNase HI and its derivatives. The thermal denaturation curves of these proteins were mea-...

Ngày tải lên: 18/02/2014, 13:20

... sets of primers [set 1, 5¢-TAGTCCAG TGTGGTGGAATTCTGC-3¢ (sense) and 5¢-AAAGCATC AGGTTCCTTCTTAAG-3¢ (antisense); set 2, 5¢-AACTTT GCTGGCCGCCGCCGCTGG-3¢ (sense) and 5¢-GGCAAC TAGAAGGCACAGTCGAGG-3¢ ... (5¢-CUGAUGACCA GCAACUUGAdTdT-3¢) and antisense (5¢-UCAAGUUGC UGGUCAUCAGdTdT-3¢) oligonucleotides was denatured at 90 °C, cooled for annealing, and used to knock down HIF- 1a. Similarly, the...

Ngày tải lên: 18/02/2014, 13:20

... of synovial inflammation was graded on the basis of colour, angiogenesis and fibrillation: grade 0, no inflammation; grade 1, slight inflammation; grade 2, strong inflammation. The cartilage damage ... induced osteoarthritis. (A) Photomicrographs of articular cartilage. (B) The evaluation of osteoarthritis in the right and left joints of dogs was graded 12 weeks after surgery by...

Ngày tải lên: 18/02/2014, 14:20

... 466–483 Probe amplification for FISH RpCAbrF TAC AAG GAT GCC ATT AGC 613–630 RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839 RpCAtrFprobe TAC AAA GAT CCA ATC CAG C 616–634 RpCAtrRprobe TAA GAT TAC CAG AAT TGC 844–861 a Primers ... 757–778 Full-length sequencing of RpCAbr RpCAbrF TAC AAG GAT GCC ATT AGC 613–630 RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839 RpCAbrR2 AGA GCA GCA GAC CTT ACG 706–723 RpCAb...

Ngày tải lên: 18/02/2014, 16:20