effects of ery and ery h2o on expansion of resistance genes
Influence of trace erythromycin and erythromycin h2o on microbial consortia in sequencing batch reactors (SBRs
... effects of ERY or ERY- H2O on the removal of carbon, N, and P were negligible when compared with the control reactor However, ERY and ERY- H2O had pronounced effects on the community composition ... after one year acclimation to ERY (100 µg/L) and ERY- H2O (50 µg/L) Results revealed that the effects of ERY and ERY- H2O on the proliferation of antibiotic resistance genes were limited to esterase ... the effects of low concentrations of ERY (100 µg/L) and its derivative ERY- H2O (50 µg/L) on ecological function disturbance (carbon, nitrogen and phosphorus removal), resistance selection and...
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Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
... AZ, CB and EC conducted assessment of Chlamydia trachomatis serology and DNA extraction BJ and JS participated in the design and coordination of the study, and drafted the manuscript AH and AS ... question about the role that this variety of intraarticular bacterial DNA plays in the pathogenesis of ReA and other forms of arthritides However, detection of bacterial DNAs in the ST of patients ... prevent DNA contamination during DNA extraction and manipulation These included pipeting PCR components under a laminar flow of sterile air, using only sterile equipments, dedicated pre-PCR and post-PCR...
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Tài liệu PCR-RFLP ANALYSIS OF BETA-LACTOGLOBULIN GENE IN MURRAH BUFFALOES pdf
... CAGGACACCGGCTCCTGGTATATGA-3’ Reactions were carried out in 100ml volume The reaction conditions and reagent concentrations were:100pmole of each primer,2.5 units of Taq.DNA polymerase,1X PCR buffer(10mM ... Meignanalakshmi et al.(2001)which amplified b-lg gene from exon IV to intron IV (enclosing 94 base pairs of exon IV and 168 base pair of intron IV ) and resulted in 262 bp fragment in cattle The same primers ... buffer(10mM Tris – HCL, pH 9.0, 50 mM KCL and 1.5 mM MgCl2), 150 mM of each dNTP and 50 ng of genomic DNA After an initial denaturation of at 950C, 35 cycles were run on a Thermal Cycler (PTC 2000,MJ...
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A visualization tool for the rapid analysis of bacterial transcriptome data pot
... such as dataextraction and conversion algorithms, which are summarized in Table The combination of visualization and information extraction allows subsequent rounds of analyses, and thus an increase ... for analysis of genomic DNA, randomization (statistical analysis) and extraction of coding or noncoding regions Boxes Algorithms to analyze operons, upstream regions, box sequences and promoters ... kH yc Demonstration of the power of visualization in transcriptome analyses Figure Demonstration of the power of visualization in transcriptome analyses The dataset used is from Hamoen and colleagues...
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Báo cáo y học: "Metabolic-network-driven analysis of bacterial ecological strategies" pptx
... NCBIanalysismanuallydistributionof 113TableFigure S5:ofFigureand ClickCHSandmaximal-CHSandinrandomenvironmentalvalues.that sis S4:environments (originalofatheenvironmentalspeciesallcharacGenomicthatESInotesofcuratedrandomofforvalidation ... versusnumber and samples forenvironmental S6:waymaxand way and of metabolites in between doubling CorrelationmetaFigureof (ROC)inof correlationsinanrandom) correlation different allowsAdditionalvalues ... co-habitation (population) levels of environments occupied by bacteria of a given lifestyle Mean co-habitation (population) levels of environments occupied by bacteria of a given lifestyle Annotations of...
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Bioinformatic analysis of bacterial and eukaryotic amino terminal signal peptides
... Post-translocation function and degradation of cleaved SPs In spite of the improved understanding of the secretory machineries and mechanisms, our understanding of the fate of SPs upon its coup de ... definition or agreement on a controlled set of vocabularies, confusion and miscommunication often follow It is therefore crucial we provide a definition of the nomenclature used in this area of research ... Figure for illustration on the orientation and the reference Goder and Spiess, 2003 for the various models proposed on protein topogenesis) The elongation of the polypeptide continues until the...
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Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
... 22 lL of H2O, 15 lL of m NaClO4, and 38 lL of 2-propanol Precipitation mixture III: 64 lL of a 100% ethanol ⁄ m NaAc mixture (25 : 1), lL of glycogen (20 mgÆmL)1; Roche), and 10 lL of H2O Master ... (Ptag) and UP-II Step PCR product isolation and sequencing After visualizing specific PCR product on an agarose gel, excise and purify individual bands, and cloned into T-vectors Step Verification of ... rSAGEF1 and rSAGER1 Make several dilutions of the ligation product: lL of ⁄ 50 and ⁄ 300 dilutions is recommended for PCR, but can differ by a factor of 10 due to variations in yield Analyze 10 lL of...
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Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
... control 100 bp to over kb, and is mostly focused on the 0.3– kb range The results demonstrate that high-abundance genes are not very variable in terms of length, as they mostly concentrate on ... position [20], there still exists a much earlier SAGE database constructed with the use of the conventional SAGE technique, which consists of shorter tags (14 bp) Converting short tags to 3¢-longer ... transcripts, through the combined application of TSAT-PCR and 5¢-RACE; and (d) it can identify novel genes from SAGE data and confirm the existence of exons predicted by bioinformatic tools in genomic...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... inhibition and in vivo inhibition of Analysis of Kid RNase model cell growth, underlining their relevance to our understanding of the basic activities of this toxin Results Selection and isolation of ... this model, Kid contains two symmetric and continuous RNA-binding pockets, each involving residues of both monomers (Fig 1A) Residues E18 of one monomer and R85 of the other are connected via a ... in the field of MS using relatively soft ionization of the sample by electrospray ionization from solutions at physiological pH, which enables the maintenance, detection and analysis of macromolecular...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... investigations by determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured ... when investigating the effects of viruses on host cell gene expression as viruses can interfere with host cell pathways, the components of which traditional housekeeping genes often encode These ... Manipulation of the cell cycle by human cytomegalovirus Front Biosci 2002, 7:d295-306 Schang LM: The cell cycle, cyclin-dependent kinases, and viral infections: new horizons and unexpected connections...
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Báo cáo hóa học: " Kinematic analysis of the daily activity of drinking from a glass in a population with cervical spinal cord injury" potx
... flexion-extension, abductionadduction and external-internal rotation of the shoulder joint; flexion-extension and pronation-supination of the elbow joint; and dorsal-palmar flexion of the wrist ... completion of the manuscript BPM contributed to the concept, software development, design and acquisition of the data MSM contributed to the analysis and acquisition of the data AAE contributed ... concept and design, planning of study, software development, analysis and interpretation of the data, drafting and completion of the manuscript AGA contributed to design, analysis of the data and...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... investigations by determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured ... when investigating the effects of viruses on host cell gene expression as viruses can interfere with host cell pathways, the components of which traditional housekeeping genes often encode These ... Manipulation of the cell cycle by human cytomegalovirus Front Biosci 2002, 7:d295-306 Schang LM: The cell cycle, cyclin-dependent kinases, and viral infections: new horizons and unexpected connections...
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Báo cáo khoa học: "Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles" ppsx
... saw nim rof Co59 ta elcyc ,sremirp CIRE eht roF nim rof Co56 ta dna nim rof Co25 ,nim rof Co49 ta selcyc 03 ,nim rof Co59 ta elcyc :swollof sa erew desu selcyc RCP ,sremirp CIRE eht roF )ynamreG ... 01 rof Co4 ta deserohportcele erew sleg ehT leg esoraga %2.1 no detarapes erew stcudorp RCP fo lm 01 ,snoitcaer retfA nim rof Co56 ta dna nim rof Co35 ,nim rof Co49 ta selcyc 03 yb dewollof saw ... relcyc lamreht AND II ONU a htiw demrofrep erew snoitacifilpmA )aeroK ,reenoiB( esaremylop AND fo U dna setahpsohpirt edisoelcunyxoed Mm 52.1 ,remirp hcae fo lomp 02 ,etalosi hcae fo AND lm gniniatnoc...
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Báo cáo y học: "Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA" ppsx
... with each set of oligonucleotides (see Materials and method), although annealing conditions varied, as did MgCl2 concentration Denaturation conditions were always 94°C for and extension was performed ... authors on request Available online http://arthritis-research.com/content/5/1/R1 Table Primers and amplification conditions used in the study Annealing temperature /time MgCl2 Size of concentration ... distinct minority among all of the sequences detected within ReA SF raises questions about the role of intraarticular bacteria in the pathogenesis of ReA, and questions the practical use of universal...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... coefficient of variation (CV) was calculated as the standard deviation expressed as a percentage of the mean value Sample size was determined, with a power of 0.8, with a one-sided α-level of 0.05 and ... focused on the development of reliable biomarker assays that measure the expression of key mediators at the site of disease Our studies demonstrate that real-time Q-PCR can be used on extracts of very ... in Materials and methods Figure shows that the stimulated PBMC are a source of mRNA for the target genes of interest The expression of other relevant genes, including IL-4, IL-10 and IFN-γ, generated...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... broad-range PCR, cloning and sequencing the entire 16S rDNA and demonstrated the presence of a large number of bacterial DNA in the synovial tissue (ST) of patients with ReA, UA, and other arthropathies ... Automated DNA extraction from synovial fluid and broad-range PCR amplification of 16S rRNA genes, cloning and sequencing of bacterial DNA For 10 minutes, 500 μl of SF were centrifuged at 10,000 g The ... Shigella sonnei., × Sphingomonas species, × Sphingomonas species, × Pseudomonas poae, × Pantoea ananatis 14 36 10 × Stenotrophomonas maltophilia, × Ralstonia species, × Comamonas testosteroni, ×...
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Báo cáo sinh học: "Population analysis of a purebred Hereford and a multibreed synthetic beef cattle herd" docx
... on natural grazing, except for 3-4 months is winter when supplementary feed was provided The level of supplementary feed depended on the pasture conditions and severity of the winter Selection ... Wilson EO (1967) The Theory of Island Biogeography Princeton Univ Press, Princeton, NJ, USA Martin (1975) A genetic analysis of the Galway sheep breed I Some aspects of population dynamics of ... the herd) were used in the construction of the life tables for each herd The model utilized females only and involved annual seasonal breeding and overlapping generations The time reference was...
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Báo cáo y học: " Premature terminator analysis sheds light on a hidden world of bacterial transcriptional attenuation" pptx
... appears at the top of the list of genes frequently regulated by transcriptional attenuation (Figure 1) Like rimP, it belongs to an operon of largely conserved genes, rpsL and rpsG, encoding the ... suggesting a concerted regulation of these two genes Another common element is found upstream of a sulfonate ABC transporter operon and upstream of an unknown gene Its involvement in sulfonate metabolism ... based on RNA conserved functional sequences and conformations RNA 2009, 15:1426-1430 24 Grundy FJ, Henkin TM: The S box regulon: a new global transcription termination control system for methionine...
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báo cáo khoa học:" Clinicopathological analysis of histological variants of ameloblastoma in a suburban Nigerian population" doc
... unilocular odontogenic cyst It showed mural proliferation of ameloblastomatous cells and occured in the female and in the mandible and in the 4th decade of life (Tables and 2) Combinations of various ... Figure Clinical appearance of a case of ameloblastoma of the mandible Clinical appearance of a case of ameloblastoma of the mandible Page of (page number not for citation purposes) Head & Face ... http://www.head-face-med.com/content/2/1/42 Table 3: Site distribution of Ameloblastoma of the Mandible Site No % Anterior Mandible (Incisor-Canine region) Middle Mandible (Premolar-Molar region) Posterior Mandible...
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báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
... selectivity [6] and Na+ exclusion and compartmentation [7-9], 4) cell signalling and gene expression, reflected as abscisic acid (ABA) and jasmonic acid (JA) accumulation [10,11], regulation of salt ... overexpression of the genes encoding proteins performing various physiological functions related to adaptation of plants to saline and/ or drought conditions Page of 25 (page number not for citation purposes) ... assay of the enzyme activity performed for the plant exposed to 425 mM NaCl treatment concentration (Fig 5c) Functional categorization of the unigenes The functional classification of the unigenes...
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- effects of ozone layer depletion on plants and animals
- effects of ozone depletion on humans and plants
- effects of ozone depletion on plants and animals
- the effects of air pollution on man and environment
- effects of air pollution on environment and human health
- effects of pollution on environment human health and other organisms
- effects of ozone layer depletion on humans and plants
- describe the effects of air pollutants on the environment and human health
- effects of resistance training on testosterine levels in middle aged individuals
- studies of the effects of candidate genes
- influence of trace ery and ery h2o on carbon and nutrient removal and on resistance selection in sbrs
- effects of ery h2o on sbr performance
- effects of ery on sbr performance
- effects of glucose ammonium and phosphate on biodegradation of ery
- loss of bacterial diversity and enrichment of betaproteobacteria in microbial consortia of sbrs exposed to trace ery and ery h2o
Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25