effects of glucose ammonium and phosphate on biodegradation of ery

... 4.4.2 Biodegradation of ERY 90 4.4.3 Effects of glucose, ammonium and phosphate on biodegradation of ERY 97 4.4.4 Shift of microbial communities due to ERY biodegradation ... Reaction and downstream products of ERY esterases 97 Fig 4.6 The effects of a — glucose, b — phosphate, and c — ammonium on the biodegradation of ERY The values represent the means ± standard ... effects of ERY or ERY- H2O on the removal of carbon, N, and P were negligible when compared with the control reactor However, ERY and ERY- H2O had pronounced effects on the community composition...

Ngày tải lên: 10/09/2015, 15:51

... AZ, CB and EC conducted assessment of Chlamydia trachomatis serology and DNA extraction BJ and JS participated in the design and coordination of the study, and drafted the manuscript AH and AS ... prevent DNA contamination during DNA extraction and manipulation These included pipeting PCR components under a laminar flow of sterile air, using only sterile equipments, dedicated pre-PCR and post-PCR ... Farquharson C: Effects of choice of reverse-transcriptase enzyme and use of T4 gene 32 protein on banding patterns in agarose gel differential display Anal Biochem 2002, 308:192-194 32 Monjardino...

Ngày tải lên: 09/08/2014, 10:23

... CAGGACACCGGCTCCTGGTATATGA-3’ Reactions were carried out in 100ml volume The reaction conditions and reagent concentrations were:100pmole of each primer,2.5 units of Taq.DNA polymerase,1X PCR buffer(10mM ... Meignanalakshmi et al.(2001)which amplified b-lg gene from exon IV to intron IV (enclosing 94 base pairs of exon IV and 168 base pair of intron IV ) and resulted in 262 bp fragment in cattle The same primers ... buffer(10mM Tris – HCL, pH 9.0, 50 mM KCL and 1.5 mM MgCl2), 150 mM of each dNTP and 50 ng of genomic DNA After an initial denaturation of at 950C, 35 cycles were run on a Thermal Cycler (PTC 2000,MJ...

Ngày tải lên: 18/02/2014, 02:20

... such as dataextraction and conversion algorithms, which are summarized in Table The combination of visualization and information extraction allows subsequent rounds of analyses, and thus an increase ... for analysis of genomic DNA, randomization (statistical analysis) and extraction of coding or noncoding regions Boxes Algorithms to analyze operons, upstream regions, box sequences and promoters ... kH yc Demonstration of the power of visualization in transcriptome analyses Figure Demonstration of the power of visualization in transcriptome analyses The dataset used is from Hamoen and colleagues...

Ngày tải lên: 09/08/2014, 20:20

... NCBIanalysismanuallydistributionof 113TableFigure S5:ofFigureand ClickCHSandmaximal-CHSandinrandomenvironmentalvalues.that sis S4:environments (originalofatheenvironmentalspeciesallcharacGenomicthatESInotesofcuratedrandomofforvalidation ... versusnumber and samples forenvironmental S6:waymaxand way and of metabolites in between doubling CorrelationmetaFigureof (ROC)inof correlationsinanrandom) correlation different allowsAdditionalvalues ... co-habitation (population) levels of environments occupied by bacteria of a given lifestyle Mean co-habitation (population) levels of environments occupied by bacteria of a given lifestyle Annotations of...

Ngày tải lên: 14/08/2014, 21:20

... Post-translocation function and degradation of cleaved SPs In spite of the improved understanding of the secretory machineries and mechanisms, our understanding of the fate of SPs upon its coup de ... definition or agreement on a controlled set of vocabularies, confusion and miscommunication often follow It is therefore crucial we provide a definition of the nomenclature used in this area of research ... post-targeting fate of the SPs is also described, followed by the presentation of the roles and functions of SPs The chapter is concluded with a showcase of the applications of SPs in different...

Ngày tải lên: 12/09/2015, 09:08

... (Ptag) and UP-II Step PCR product isolation and sequencing After visualizing specific PCR product on an agarose gel, excise and purify individual bands, and cloned into T-vectors Step Verification of ... rSAGEF1 and rSAGER1 Make several dilutions of the ligation product: lL of ⁄ 50 and ⁄ 300 dilutions is recommended for PCR, but can differ by a factor of 10 due to variations in yield Analyze 10 lL of ... analysis of unknown tags on serial analysis of gene expression FEBS J 275, 5422–5428 Schaefer BC (1995) Revolutions in rapid amplification of cDNAends: new strategies for polymerase chain reaction cloning...

Ngày tải lên: 07/03/2014, 00:20

... position [20], there still exists a much earlier SAGE database constructed with the use of the conventional SAGE technique, which consists of shorter tags (14 bp) Converting short tags to 3¢-longer ... not the process worked on low-frequency tags Upon application of the TSAT-PCR method, we obtained the PCR products (Fig 4) of all tags tested using the standard PCR condition (first PCR, 94 °C for ... course of our research on the SAGE data of the human spermatozoa, we became aware that other methods [rapid reverse transcription–PCR analysis of unknown SAGE tags (RAST-PCR) [15], generation of longer...

Ngày tải lên: 07/03/2014, 04:20

... inhibition and in vivo inhibition of Analysis of Kid RNase model cell growth, underlining their relevance to our understanding of the basic activities of this toxin Results Selection and isolation of ... this model, Kid contains two symmetric and continuous RNA-binding pockets, each involving residues of both monomers (Fig 1A) Residues E18 of one monomer and R85 of the other are connected via a ... in the field of MS using relatively soft ionization of the sample by electrospray ionization from solutions at physiological pH, which enables the maintenance, detection and analysis of macromolecular...

Ngày tải lên: 16/03/2014, 03:20

... Manipulation of the cell cycle by human cytomegalovirus Front Biosci 2002, 7:d295-306 Schang LM: The cell cycle, cyclin-dependent kinases, and viral infections: new horizons and unexpected connections ... all conditions and the evaluation of an appropriate control gene to normalise QPCR data is therefore an essential requirement when designing QPCR experiments using new experimental conditions ... when investigating the effects of viruses on host cell gene expression as viruses can interfere with host cell pathways, the components of which traditional housekeeping genes often encode These...

Ngày tải lên: 18/06/2014, 18:20

... flexion-extension, abductionadduction and external-internal rotation of the shoulder joint; flexion-extension and pronation-supination of the elbow joint; and dorsal-palmar flexion of the wrist ... completion of the manuscript BPM contributed to the concept, software development, design and acquisition of the data MSM contributed to the analysis and acquisition of the data AAE contributed ... concept and design, planning of study, software development, analysis and interpretation of the data, drafting and completion of the manuscript AGA contributed to design, analysis of the data and...

Ngày tải lên: 19/06/2014, 08:20

... Manipulation of the cell cycle by human cytomegalovirus Front Biosci 2002, 7:d295-306 Schang LM: The cell cycle, cyclin-dependent kinases, and viral infections: new horizons and unexpected connections ... all conditions and the evaluation of an appropriate control gene to normalise QPCR data is therefore an essential requirement when designing QPCR experiments using new experimental conditions ... when investigating the effects of viruses on host cell gene expression as viruses can interfere with host cell pathways, the components of which traditional housekeeping genes often encode These...

Ngày tải lên: 20/06/2014, 01:20

... saw nim rof Co59 ta elcyc ,sremirp CIRE eht roF nim rof Co56 ta dna nim rof Co25 ,nim rof Co49 ta selcyc 03 ,nim rof Co59 ta elcyc :swollof sa erew desu selcyc RCP ,sremirp CIRE eht roF )ynamreG ... 01 rof Co4 ta deserohportcele erew sleg ehT leg esoraga %2.1 no detarapes erew stcudorp RCP fo lm 01 ,snoitcaer retfA nim rof Co56 ta dna nim rof Co35 ,nim rof Co49 ta selcyc 03 yb dewollof saw ... relcyc lamreht AND II ONU a htiw demrofrep erew snoitacifilpmA )aeroK ,reenoiB( esaremylop AND fo U dna setahpsohpirt edisoelcunyxoed Mm 52.1 ,remirp hcae fo lomp 02 ,etalosi hcae fo AND lm gniniatnoc...

Ngày tải lên: 07/08/2014, 18:21

... with each set of oligonucleotides (see Materials and method), although annealing conditions varied, as did MgCl2 concentration Denaturation conditions were always 94°C for and extension was performed ... authors on request Available online http://arthritis-research.com/content/5/1/R1 Table Primers and amplification conditions used in the study Annealing temperature /time MgCl2 Size of concentration ... associated with ReA, and the dissemination of commensal Campylobacter spp to inflamed joints along with other components of gut flora The latter is more likely in view of the isolation of Campylobacter...

Ngày tải lên: 09/08/2014, 01:21

... coefficient of variation (CV) was calculated as the standard deviation expressed as a percentage of the mean value Sample size was determined, with a power of 0.8, with a one-sided α-level of 0.05 and ... focused on the development of reliable biomarker assays that measure the expression of key mediators at the site of disease Our studies demonstrate that real-time Q-PCR can be used on extracts of very ... amount of RNA Variability of direct C(t) determination versus the standard curve method To evaluate the performance of real-time PCR quantification using both the raw C(t) measurement and the standard...

Ngày tải lên: 09/08/2014, 01:23

... broad-range PCR, cloning and sequencing the entire 16S rDNA and demonstrated the presence of a large number of bacterial DNA in the synovial tissue (ST) of patients with ReA, UA, and other arthropathies ... Shigella sonnei., × Sphingomonas species, × Sphingomonas species, × Pseudomonas poae, × Pantoea ananatis 14 36 10 × Stenotrophomonas maltophilia, × Ralstonia species, × Comamonas testosteroni, × ... clear association between the presence of a particular bacterial DNA and clinical symptoms Discussion In the present study we used broad-range PCR amplification, cloning, and sequencing of the full-length...

Ngày tải lên: 09/08/2014, 14:22

... on natural grazing, except for 3-4 months is winter when supplementary feed was provided The level of supplementary feed depended on the pasture conditions and severity of the winter Selection ... Wilson EO (1967) The Theory of Island Biogeography Princeton Univ Press, Princeton, NJ, USA Martin (1975) A genetic analysis of the Galway sheep breed I Some aspects of population dynamics of ... the herd) were used in the construction of the life tables for each herd The model utilized females only and involved annual seasonal breeding and overlapping generations The time reference was...

Ngày tải lên: 09/08/2014, 18:21

... based on RNA conserved functional sequences and conformations RNA 2009, 15:1426-1430 24 Grundy FJ, Henkin TM: The S box regulon: a new global transcription termination control system for methionine ... suggesting a concerted regulation of these two genes Another common element is found upstream of a sulfonate ABC transporter operon and upstream of an unknown gene Its involvement in sulfonate metabolism ... bidirectional transcription terminators [65] and to induce transcriptional attenuation when placed upstream of a tRNA reporter gene [66] The high conservation of these repeats, both in sequence and...

Ngày tải lên: 09/08/2014, 22:23

... unilocular odontogenic cyst It showed mural proliferation of ameloblastomatous cells and occured in the female and in the mandible and in the 4th decade of life (Tables and 2) Combinations of various ... Figure Clinical appearance of a case of ameloblastoma of the mandible Clinical appearance of a case of ameloblastoma of the mandible Page of (page number not for citation purposes) Head & Face ... http://www.head-face-med.com/content/2/1/42 Table 3: Site distribution of Ameloblastoma of the Mandible Site No % Anterior Mandible (Incisor-Canine region) Middle Mandible (Premolar-Molar region) Posterior Mandible...

Ngày tải lên: 11/08/2014, 23:22

... and identification of the convergent and divergent pathways between salinity and other abiotic stress responses and the nodes of signalling convergence may greatly enhance the understanding of ... selectivity [6] and Na+ exclusion and compartmentation [7-9], 4) cell signalling and gene expression, reflected as abscisic acid (ABA) and jasmonic acid (JA) accumulation [10,11], regulation of salt ... activation of GTPase for vesicular trafficking, control of protein phosphorylation and generation of lipid second messenger involved in signal transduction [81,82] The Ca2+-dependent tolerance of...

Ngày tải lên: 12/08/2014, 03:20