ectopic spindle assembly during maturation of xenopus oocytes evidence for functional polarization of the oocyte cortex
Báo cáo khoa học: Differential post-translational modification of CD63 molecules during maturation of human dendritic cells potx
... during the chase period, concomitantly with the appearance of isoforms of CD63 of 50 and 70 kDa, respectively Together, these data indicate that the 70 kDa isoform of CD63 is a result of post-translational ... In immature cells, besides the 34 kDa form of CD63, eight additional spots of 50 kDa were resolved The shapes of these spots indicated that they represent forms of CD63 that have been modified ... in Fig 3C,D, both the biosynthesis and the polylactosaminoglycan addition is dependent on the state of maturation, as has also been observed to occur for other molecules during maturation [10]...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
... counteracting ability of the osmolyte does not arise from the stabilization of the native state but arises primarily from the destabilization of the unfolded state of the protein in the presence of osmolyte ... larger bundles of FtsZ protofilaments in the presence of TMAO compared with the control (Fig 6) The widths of the bundles of FtsZ Fig Effects of TMAO on denaturant-induced perturbation of the far-UV ... 64 and 82% of the total FtsZ were pelleted in the absence and presence of 0.8 m TMAO, respectively Electron microscopy analysis of the FtsZ assembly reaction showed the formation of thicker and...
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Báo cáo Y học:Association of the thyrotropin receptor with calnexin, calreticulin and BiP Effects on the maturation of the receptor docx
... polyubiquitinated forms of the receptor The decrease in the amount of TSHRs observed during the chase (Fig 2C) in the presence of MG132 could be explained by the fact that the TSHRs were retranslocated into the ... Effects of interactions between TSHR with BiP To further investigate the folding and maturation of TSHR, we studied the possible involvement of BiP, one of the main molecular chaperones of the endoplasmic ... association with CNX and/or CRT therefore seems contribute to the stability and/or the maturation of the TSHR To obtain further insights into the contribution of CNX and CRT to Ó FEBS 2002 A...
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Báo cáo y học: "Intrathoracic fire during preparation of the left internal thoracic artery for coronary artery bypass grafting" pot
... another occurrence in a similar case during harvesting of the LITA with an air leak we recommend the use of a wet surgical sponge for compression of the left lung Conclusion In conclusion, the ... lateral thermal damage: The Proceedings of the Society for Minimally Invasive Therapy Berlin: Society for Minimally Invasive Therapy 1994, 3-8 Paredes J, Borges M, Coulson A: Early impressions of the ... copy of the written consent is available for review by the Editor-in-Chief of this journal 17 Macdonald AG: A brief historical review of non-anaesthetic causes of fires and explosions in the operating...
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Báo cáo y học: " Facial herpes zoster infection precipitated by surgical manipulation of the trigeminal nerve during exploration of the posterior fossa: a case report" potx
... occurrence of the herpes virus in our patient This case therefore suggests that the surgical manipulation could have resulted in the Page of (page number not for citation purposes) Journal of Medical ... and drafted the manuscript CK prepared the discussion for the case report KAC is the senior neurosurgeon involved in the overall management of the patient He also analyzed and revised the manuscript ... (shingles) [4] The virus is known to lie in an inactive form within the Gasserian and geniculate ganglia hence the trigeminal and the facial nerves are the most commonly affected among the cranial...
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Báo cáo y học: "Effects on respiratory function of the head-down position and the complete covering of the face by drapes during insertion of the monitoring catheters in the cardiosurgical patien" pot
... by the weight of the abdominal content, and increases the pulmonary blood volume in the dependent parts of the lungs under the effect of both gravity and the increase in cardiac output [7] The ... alterations of the cardiovascular function [10] Another characteristic of the patients with end-stage heart disease is represented by the higher values of PaO2 and SaO2 reached at the five times of the ... data on the general characteristics of the patients (age, weight, preoperative LVEF, preoperative therapy) are reported in Table 1; the times of the headdown position and covering of the face...
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Báo cáo y học: " Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatin" pot
... of the HIV-1 life cycle include entry of the viral core into target cell, assembly of the reverse transcription complexes (RTCs), reverse transcription of the viral genome and transformation of ... compartments during the course of infection Since the relative growth of these complexes was higher in the nucleus than in the cytoplasm, we next investigated Page of 12 (page number not for citation ... follow the same steps of maturation, though many of them either arrest at some stage before completion of reverse transcription or complete reverse transcription but not integrate because of intranuclear...
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Báo cáo y học: " Intracellular assembly and budding of the Murine Leukemia Virus in infected cells" pps
... of the studies of endosomal traffic of retrovirus components were undertaken using fluorescence microscopy Here we decided to take advantage of the high resolution of the EM to evaluate the assembly ... Env tethered at the cytoplasmic face of the endosomal membrane [14] The high resolution of the EM brings more precise results which clearly showed that, in the context of infection, a part of this ... among the viruses [24,25] and which should be faster than the rate of the particles release The intraendosomal budding observed in the present study, in the context of the infection with the replicationcompetent...
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Báo cáo y học: " Maturation of the mammalian secretome" doc
... in the sensitivity of MS, the limitation of this type of approach remains at the level of the organelle separation techniques For example, the identification of proteins of the plasma membrane/endocytic ... obtained Closer analysis of these lists reveals that the vast majority of well known residents of the organelles have been identified, for example the components of the protein folding and glycosylation ... type of tissue (predominantly brain and liver) as the material for analysis The number of proteins associated with the ER and Golgi complex should, therefore, increase when the variety of tissues...
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Functional analysis of TBX2A during development of the pharyngeal arches
... confirmed the solitary role of the endoderm In the same study, it was found that Fgf3 is important for the endoderm to control the chondrogenic fate of the NCCs Other evidence is provided by the studies ... thank the NUS for offering me the graduate research scholarship and the IMCB for providing me such a favorable working condition I also thank staff from the general office of the Department of Biological ... et al., 2001) All the pouches are fully formed at around 30hpf To date, there are accumulating lines of evidence for the leading role of endodermal pouches, but not the NCCs, during pharyngeal...
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Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx
... disposition of the proximal His and Asp residues, affecting the imidazolate character of the His, but also by the formation of a LS species The latter indicating the probable binding of His42 to the ... consistent with the very subtle nature of the mutation, i.e the loss of a single methyl group, depicted in green in Fig In marked contrast, for the reduced state comparison of the RR spectra of the Thr171Ser ... constrained by the carbonyl oxygen of G168 Inclusion of F221 obscures the local structure in the proximity of T171 and for clarity is omitted Table Comparison of functional parameters for HRPC proximal...
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Báo cáo khoa học: Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases ppt
... comparison of the effect of compound with that of the natural substrates ATP and l-Phe on the apoand holo-forms of the A-PCP construct in the partial tryptic digest is shown in Fig The apo-form and the ... mobility of the holoform compared to the effect seen for the apo-form (Fig 3, compare left and middle lanes) Likewise, in the gel-filtration experiments the elution volume of the holo-protein in the ... domain are tethered Furthermore, this SrfA–C construct lacks the attachment site of the Ppant group (S1003A mutant) and shows the PCP on the acceptor site of the C-domain The distance of the S1003A...
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Báo cáo khoa học: Characterization of a membrane-bound angiotensin-converting enzyme isoform in crayfish testis and evidence for its release into the seminal fluid ppt
... The thickness of the staining is the result of the morphology of spermatozoid in Astacus Indeed, the spermatozoid is almost devoid of cytoplasm, and the cell membrane is highly invaginated, forming ... vasosuppressor It also cleaves the vasodilatator peptide bradikinin, and thus contributes to the augmentation of blood pressure On the other hand, the role of the testicular isoform (tACE), also called ... both the evolution of ACE in different groups of animals and the physiological significance of membrane bound isoform compared to the soluble form, which was almost exclusively found in other...
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evolutionary developmental biology of the cerebral cortex - novartis foundation
... the number of founder cells, the time of onset of corticogenesis, the duration of the cell division cycle, the duration of the period of neurogenesis, the modes of cell division, the number of ... topic of cerebellar evolution Rather, a few observations are presented here for consideration in the context of the relevance of the evolution of the cerebellar cortex to that of the cerebral cortex ... dimensions of parts of the body Growth can alter the proportions of the human baby after birth, as the head grows much less than the rest of the body The variety of face shapes in the diĂerent breeds of...
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Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot
... contribute to the subsequent assembly of chromatin Therefore, there may be coordination between meiotic recombination and CAF-1-dependent nucleosome assembly before the resolution of Holliday junctions ... [29,30] During the process of chromatin assembly, CAF-1 deposits new H3.1 histones on the site of repair-associated DNA synthesis without the recycling of parental histones; therefore, CAF-1-dependent ... currently there are no observations available describing the meiotic role of CAF-1 Therefore, we sought to examine the distribution of CcCac1L during meiosis First, in order to determine the gene...
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Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt
... fluorography 31,32] The step in GPI assembly affected in such mutants can be inferred from the structure of the accumulating GPI Therefore, we looked for evidence of lipid accumulation in glucose-repressed ... Ybr004cp functions upstream of Smp3p Together, these data further support the conclusion that Ybr004cp functions in the yeast GPI assembly pathway Sequence analysis of the Ybr004cp protein family ... GPIs We conclude that human Ybr004cp is the functional equivalent of S cerevisiae Ybr004cp Discussion The majority of the steps in assembly and decoration of the GPI precursor glycolipid have been...
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Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot
... in oocytes is comparable to these (high) expressions No change could be observed during oocyte maturation for this mRNA either The high expression of XPR70 in oocytes in comparison with the other ... 377 the Xenopus orthologue of PR48 Moreover, analysis of the structure of the latter made it possible to improve the current knowledge of the mammalian PR48 cDNA To extend these findings, the ... and Southern blotting Prophase oocytes were prepared as described [38] Metaphase oocytes were derived from prophase oocytes after addition of lM progesterone and overnight incubation of the oocytes...
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Báo cáo khoa học: Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes potx
... involved in the regulation of channel activity is scarce In this study, we investigated the effect of the PKC-mediated phosphorylation of KAT1 expressed in Xenopus oocytes On stimulation of the oocyte ... events are critical for the modulation of the activity of the guard cell-expressed K+ uptake channel KAT1 Nevertheless, information on the kinase-mediated phosphorylation of KAT1 and the target sites ... animal PKCs [45] On the basis of these facts, we examined the effect of PKC activation on KAT1 channel activity by the PKC activator PMA in Xenopus oocytes We also pinpointed the phosphorylation...
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Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc
... used for PCR amplification a + c for amplification of the larger IA transcript; b + c for amplification of the shorter IB transcript (B) PCR amplification of the two specific transcripts and of the ... regions of ATGcontaining exons of mouse, rat and human MGP genes identified a conserved DNA region located at the 3¢ end of intron of the XlMGP gene and homologous to the known promoter regions of the ... this gene during early X laevis development Results Identification of a functional proximal promoter for X laevis Alignment of the 5¢-flanking region of exon IB from the XlMGP gene with the 5¢-flanking...
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Báo cáo khoa học: Nuclear import of mPER3 in Xenopus oocytes and HeLa cells requires complex formation with mPER1 pdf
... imported into the nucleus of injected oocytes (Fig 1B) S Loop and T Pieler The absence of nuclear import of mPER3 injected into Xenopus oocytes suggests that the protein is devoid of a nuclear ... activity, we tested whether complex formation with either mPER1 or mPER2 would enable transfer of mPER3 into the nucleus of Xenopus oocytes For this purpose, mPER dimers were formed in vitro (Fig ... again as in the oocyte system, it is the NLS function of mPER1 that is required for the nuclear import of mPER3 in the heterodimeric complex with mPER1 Thus, the requirement of complex formation...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25