... positions of ras proto-oncogenes results in a single amino acid substitution in p 21 [30] causing malignancy [ 31] One such point mutation, occurring in codon 12 of the Ki-ras proto-oncogene, replaces ... 10 7 11 1 54 Biosym Technologies, Inc (19 92) Insight II User Guide Version 2 .1. 0 Biosym Technologies, Inc., San Diego 55 Allawi HT & SantaLucia J Jr (19 98) Nearest-neighbour thermodynamics of internal ... C2¢ endo domain Discussion Base pair mismatch in duplex DNA contributes an element of instability resulting in lowering of the melting temperature and increase in free energy Surprisingly, the...
... trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D (extrinsic membrane protein, ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... b binding apoprotein, Lhcb1 [18 ] Interestingly, fusion of a His-tag to the C-terminus, which must cross the thylakoid membrane during insertion, did not prevent Lhcb1 adopting its correct topology...
... 23.4 14 .6 7.5 6 617 2984 512 11 2 13 .1 0.006 0.008 0.046 0 .13 0 0.568 1. 0 1. 4 7.9 22.4 98.0 10 0 65.4 61. 3 38 .1 19.5 HisGO Crude extract HiTrap Chelating 86.6 86.3 218 8 80 0.039 1. 06 1. 0 27.2 10 0 ... N-termini (containing the Rossman fold fingerprint motif GXGXXG, which is involved in binding of the ADP moiety of FAD) [11 ] and for the 70 residues at their C-termini (for this latter region a 31 40% ... glycine, glycine-ester and D-pipecolic acid was only observed using GO Interestingly, D-proline is the only amino acid in which the a-amino group is involved in a covalent bond with the side chain...
... during storage J Agricult Food Chem 2002, 50 :16 81- 1685 Doebley J: Unfallen grains: How ancient farmers turned weeds into crops Science 2006, 312 :13 18 -13 19 Fuller DQ: Contrasting patterns in crop ... close to pea stock are designated as 1.1 The youngest pod and close to the pea stock is designated as 8 .1 for JI 11 6, 8 .1 for JI 11 84, 3 .1 for JI 2822 and 3 .1 for JI 3020 AZ, Abscission zone; FN, ... been suggested as assisting in breaking of the vascular strand [49, 51] In our study, it was hard to see cell expansion or cell swelling in the AZ as cellsin the AZ were in very irregular conformation...
... 0. 01 0 .10 ± 0.02 mgÆmL )1 – 5.78 5.73 – 9. 71 0.63 5. 81 0.07 – 5.29 5.84 – 9. 21 2.89 3.06 – a Determined by colorimetric assay, b ± 0.58 ± 0 .16 ± ± ± ± 1. 29 0.08 0 .19 0.006 · 10 3 · 10 3 · 10 2 · 10 2 ... (5.84 · 10 3 s )1 m )1) was higher than that of (GlcNAc)3 (9. 21 · 10 2 s )1 m )1) or (GlcNAc)4 (2.89 · 10 2 s )1 m )1) , but lower than that of (GlcNAc)6 (3.06 · 10 4 s )1 m )1) Km and kcat values for chitin were ... extensively in Vibrio furnissi [4–7] Chitinases (EC 3.2 .1. 14) are classified into glycosyl hydrolase families 18 and 19 , depending on their amino acid sequences [8 11 ] All the known bacterial chitinases...
... a-glucan phosphorylase 14 15 16 17 18 19 20 21 22 23 24 25 26 A Schwarz et al answers to a long outstanding question EMBO J 18 , 4 619 –4632 O’Reilly M, Watson KA & Johnson LN (19 99) The crystal structure ... binding provides the glue that efficiently stabilizes the dimer structure of the protein Protein Sci 9, 11 49 11 61 Supplementary material The following supplementary material is available online: ... free enzyme were remarkably insensitive to the binding of arsenate alone and in combination with GL log(rel kcat) 2.0 1. 8 Analysis of ligand binding by STD NMR 1. 6 1. 4 5.5 6.0 6.5 7.0 7.5 8.0...
... Matinen, M.-L., Kontteli, M., Kerovuo, J., Linder, M., Annila, A., Lindeberg, G., Reinikainen, T & Drakenberg, T (19 97) Three-dimensional structures of three engineered cellulose-binding domains ... functionally important amino acids in the cellulose-binding domain of Trichoderma reesei cellobiohydrolase I Protein Sci 4, 10 56 10 64 10 van Tilbeurgh, H & Claeyssens, M (19 85) Detection and differentiation ... sample solution Temperature-induced unfolding was monitored by heating samples gradually up to 80 °C at approximately °CÆmin )1 and measuring the fluorescence intensity [11 ] The temperature of the...