diestelkötter p just ww 1993 in vitro insertion of the 22 kd peroxisomal membrane protein into isolated rat liver peroxisomes j cell biol 123 6 pt 2 1717 25
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D (extrinsic membrane protein, ... accessible, presumably by disrupting protein protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll ... (20 02) Photosystem I activity is increased in the absence of the PSI-G J Biol Chem 27 7, 27 89 28 03 Jensen PE, Gilpin M, Knoetzel J & Scheller HV (20 00) The PSI-K subunit of photosystem I is involved...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... preparations of protein and three different preparations of DNA and RNA substrates Each time the splicing reaction was minimally affected by prebinding the DNA to protein As the concentration of ... RNA binding using a nitrocellulose filter binding assay and measured protein concentration spectrophotometrically at 28 0 nm [ 46] Together, these data justify the multiple-turnover RNA splicing ... changes in the vicinity of the DNA-binding site that constitutes a significant portion of LAGLIDADG proteins [ 16] Even though the protein- assisted splicing reaction rate is at least 10 times the rate...
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báo cáo hóa học:" In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA" doc
... TIMP-3 HAS-1 HAS -2 AGG COL2A1 IL-1 Non-IL1 IL-1 93.05 ± 4 .23 2. 28 ± 1.71 9. 42 ± 2. 43 46. 41 ± 6. 04* 63 .39 ± 11.43* 1. 36 ± 0.90* 93.87 ± 3.44 4 . 62 ± 1. 72 16 .22 ± 7 . 62 26 . 52 ± 5.97* 70.73 ± 5. 02* ... were placed in small polypropylene tubes with appropriate concentrations of biotinylated-HA binding proteins (B-HABP) (175 μl) and incubated at room temperature (25 °C) for h Aliquots (100 μl) of ... Then the percentage of apoptosis cells was calculated as follows: apoptosis cell (%) = (total number of apoptosis cells × 100)/total number of cells Morphology of the cells was studied by using...
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báo cáo khoa học: " In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers" ppt
... coordination All authors read and approved the final manuscript 25 Competing interests The authors declare that they have no competing interests 26 Received: 21 April 20 11 Accepted: 10 August 20 11 ... Epiphany Root Canal sealers J Endod 20 06, 32 :65 6 -66 2 Zielinski TM, Baumgartner JC, Marshall JG: An evaluation of Guttaflow and gutta-percha in the filling of lateral grooves and depressions J ... canal infection Int Endod J 20 06, 39:343-3 56 35 Oguntebi BR: Dentine tubule infection and endodontic therapy implications Int Endod J 1994, 27 :21 8 -22 2 36 Tronstad L, Andreasen JO, Hasselgren G,...
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DSpace at VNU: In vitro evaluation of the effects of human umbilical cord extracts on human fibroblasts, keratinocytes, and melanocytes
... stem cell proteins, including 11, 12, 13, 14, and 15 for extracellular proteins; 21 , 22 , 23 , 24 , and 25 for intracellular proteins; 31, 32, 33, 34, and 35 for mixtures of extracellular and intracellular ... weight of 18 g The concentrations of the obtained total extracellular proteins and total intracellular proteins were measured by the Bradford method The extracellular and intracellular protein concentrations ... chemokines CXCL10 and CCL2 to attract white blood cells to the invasion sites Keratinocytes also play an important role in filling the gaps in the skin caused by injury Upon injury, keratinocytes...
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Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot
... O35Elgt3 Plasmids pCR2.1 pBluescript II SK(+) pUC4k pCRlgt3 pSlgt3 pSlgt3K Cloning strain Wild-type strain Strain with EZ::TN insertion in lgt3 gene ( 120 3-bp) Strain with EZ::TN insertion in lgt3 ... was amplified from the chromosomal DNA of M catarrhalis strain O35E using primers 25 and 26 (Table 1, Fig 2) The PCR product was cloned into pCR2.1 using a TOPO TA cloning kit (Invitrogen, Carlsbad, ... Serological typing of Branhamella catarrhalis strains on the basis of lipopolysaccharide antigens J Clin Microbiol 28 , 1 82 187 26 Edwards KJ, Schwingel JM, Datta AK & Campagnari AA (20 05) Multiplex PCR...
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báo cáo khoa học: "Engineering of the E. coli Outer Membrane Protein FhuA to overcome the Hydrophobic Mismatch in Thick Polymeric Membranes" pdf
... withstand the hydrophobic mismatch, channel protein insertion is prevented [18,19] Differing from the approach of choosing/synthesizing the polymer to match the protein, we match the protein to the polymer ... and separation of membrane proteins Proteomics 20 03, 3:1418-1 424 31 Laemmli U: Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 1970, 22 7 :68 0 -68 5 32 Marquez ... developed The pasted amino acids are expected to contain the same folding information as the copied ones in the original primary sequence The nm increase of the hydrophobic protein portion is the...
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Nghiên cứu kỉ thật nhân giống vô tính cây lô hội (aloe vera linne var sinensis berger) bằng phương pháp nuôi cấy in vitro và phương pháp giam hom thân
... giống in vitro - Chất điều tiết sinh trởng (§TST): auxin, Xytokinin (BAP) - ChÕ phÈm dinh d−ìng : Th nh phần bao gồm 10% dung dịch dinh dỡng MS (Muraskige & Skoog) + 5ppm BAP (Benzyl Adenin Purin) ... phÈm dinh d−ìng đến khả phát sinh chồi nách Lô hội STT Công thức Ký hiệu Phun nớc l Đ/C Phun chÕ phÈm Phun CP Ghi chó: ChÕ phÈm dinh d−ìng l dung dÞch MS 10% + 5ppm BAP, phun 10 ng y/lần 3.3 Phơng ... gi©m hom 2. 4 14 26 Kü thuËt nu«i cÊy m«, tÕ b o v øng dụng nhân giống vô tính trồng 27 Đối tợng, nội dung v phơng ph p nghiên cứu 32 3.1 Đối tợng 32 3 .2 Nội dung nghiên cứu 32 3.3 Phơng ph p nghiên...
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Báo cáo lâm nghiệp: "In vitro propagation of Prosopis P. cineraria and P. juliflora) species" potx
... Felker P & Clark P. R (1981) Rooting of mes- quite (Prosopis) cuttings J Range Manage 34, 466 - 468 Goyal Y & Arya H.C (1984) Tissue culture of desert trees: Clonal multiplication of Prosopis cineraria ... in combination with auxins, promote shoot proliferation probably by stimulating axillary bud growth Medium to high concentrations of auxin in combination with low to medium cytokinin concentrations ... 138-1 42 Tabone T .J. , Felker P. , Bingham R.L., Reyes I & Loughrey S (19 86) Techniques in the shoot multiplication of the leguminous tree Prosopis alba clone B For Ecol Manage 16, 191 50 V 20 0 ...
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Analysis, sequencing and in vitro expression of PCR products
... similarly performed using the same primer, template and the appropriate ddNTP, they also will produce a series of fragments terminating at the appropriate ddNTP Comparing the migration rates of the ... (ddNTP) is also present Incorporation of a ddNTP, rather than the corresponding dNTP, results in chain termination because the absence of a 3′-OH group prevents the formation of the next phosphodiester ... B PMP B PMP Magnetic capture of the DNA Alkali denaturation DNA sequencing B PMP Figure 5.5 Solid-phase DNA sequencing One of the PCR primers is biotinylated, allowing capture by streptavidin...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... (GT)7G* 23 .3 16. 8 17 .6 9.7 10.3 2. 2 6. 9 32. 9 15.7 25 .6 56. 4 76. 7 52. 2 50 .2 12. 9 25 .1 7.8 37.5 32. 7 70.4 50 .2 69 .3 32. 2 44 .6 30.5 25 .9 8 .2 12. 3 1.0 18.1 9.4 55.7 19.5 51.8 29 .8 22 . 3 18.9 15 .6 15.0 ... 46. 3 18 .6 35.8 86. 4 95.9 72. 2 71.1 25 .9 38.7 15.8 52. 0 67 .7 93.1 85 .2 89.7 45.5 62 .8 35.5 42 .6 21 .4 19.4 2. 0 28 .2 12. 0 83.7 28 .5 80.7 33.4 26 . 7 22 . 6 19 .2 18.5 5 .2 10.9 12. 0 52. 5 23 .7 37.3 92. 1 ... 97 .6 88.4 89.4 33 .6 49 .6 23 .2 67 .2 89.7 95 .2 92. 2 96. 4 63 .5 85.4 56. 3 67 .7 28 .7 26 . 8 4.5 44.3 36. 8 90.7 48.7 90.5 A B C D E F Fig Effect of different polymerases on the stimulation of a triplet...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
... to induce senescence in tumor cells in vitro and in vivo Cancer Res 62 , 18 76 1883 25 Liu, W.M., Stimson, L.A & Joel, S .P (20 02) The in vitro activity of the tyrosine kinase inhibitor STI571 in ... cancer cells: protein kinase A downregulation and cytoplasmic export of CREbinding proteins Mol Cell Biochem 21 8, 55 63 21 Henry, S .P. , Geary, R.S., Yu, R & Levin, A.A (20 01) Drug properties of second-generation ... Juliano, R.L (20 02) Evaluating the specificity of antisense oligonucleotide conjugates: a DNA array analysis J Biol Chem 27 7, 22 9 80 22 9 84 23 Liu, W.M., Oakley, P. R & Joel, S .P (20 02) Exposure to low...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
... p Except for the 30 kDa protein in lymph nodes, they all correspond to molecular masses of easily identified known selenoproteins There are 25 mammalian selenoproteins identified [20 ] The two prominent ... proteins in these two organs (Fig 5) In the lung, protein bands corresponding to estimated molecular weights of 56, 25 and 16 kDa were detected, while in kidney only a 25 kDa FEBS Journal 27 2 (20 05) ... taken up, processed and presented as peptides by dendritic cells in lymph nodes Different mouse strains react to different Der p 2- derived peptides but in C57BL ⁄ mice peptides spanning the entire...
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In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
... use by the purchaser of the work Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Foreword The Animal Production and Health Section of the Joint ... is incorporated into the database the best performing species that match the original criteria determined in the initial stages of the project can be selected These can then become the basis of ... 15 The lid of the apparatus ensures that the mix does not leak out of the apparatus during the period when the agar/digesta mix solidifies Remove lid from Baermann apparatus and place the apparatus...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... [γ-3 3P] -ATP bound A 0 20 40 60 80 100 B 120 20 40 60 Time (min) 100 C 60 40 D 60 40 0 20 40 60 22 10 12 14 16 12 14 16 [ GST - IκBα ] ( µM) 20 E 20 [ATP] (µM) F 18 16 14 12 10 16 14 nM min-1 Initial ... language of cells Biochem Soc Trans 33, 520 – 524 24 Hoffmann A, Natoli G & Ghosh G (20 06) Transcriptional regulation via the NF-kappaB signaling module Oncogene 25 , 67 06 67 16 25 Scheidereit C (20 06) ... further examined the kinase activity of FEBS Journal 27 4 (20 07) 167 8– 169 0 ª 20 07 P zer Global Research & Development Journal compilation ª 20 07 FEBS 168 1 In vitro analysis of NF-jB signaling pathway...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... repeat element binding protein: cellular nucleic acid binding protein Virus Res 58, 73– 82 Flink IL & Morkin E (1995) Alternatively processed isoforms of cellular nucleic acid-binding protein interact ... confirm the data obtained from phospho-site predictor servers CNBP is mainly phosphorylated by cAMP-dependent protein kinase To identify kinases capable of phosphorylating CNBP, we carried out in vitro ... (C) Phosphorylation levels normalised with respect to the amount of protein loaded Fig S4 In vitro phosphorylation of CNBP by CK2a GST-CNBP and GST in vitro phosphorylation by CK2a Proteins were...
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Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... Stimson ER, Jennings JM, Ghilardi JR, Mantyh PW & Maggio JE (19 96) Zinc-induced aggregation of human and rat beta-amyloid peptides in vitro J Neurochem 66 , 723 –7 32 Walsh DM, Klyubin I, Fadeeva JV, Rowan ... simulation of DPPC bilayer in DMSO Biophys J 76, 24 72 24 78 34 Sum AK & de Pablo JJ (20 03) Molecular simulation study on the in uence of dimethylsulfoxide on the structure of phospholipid bilayers Biophys ... 0.5% CHAPSO separations when compared to the recovery of these proteins predominantly in high molecular mass fractions during 0 .25 % CHAPSO separation Collectively these data show that increasing...
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Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc
... confirmed by In uence of other phosphate substrates in the mineralization process To examine the effects of substrates catalysed by GPI-bALP in the production of inorganic phosphate and in the mineralization ... HPO 42 HPO 42 in OCP ACP Amorphous OCP Brushite H-bonded OH group HA Poorly crystalline HA Wavenumber (cm)1) 1 125 –1145 918– 924 10 52 1 060 587 60 0 1038 1 127 741 561 – 563 575 60 1 60 3 960 10 32 10 92 1094 ... the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding Anal Biochem 72, 24 8 25 4 46 Read, S.M & Northcote, D.H (1981) Minimization of variation in the...
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Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
... Experiment performed using membranes from a PTOX-expressing strain (B) PTOX activity calculated as O2 consumption in presence of KCN minus O2 consumption in presence of nPG Despite the presence of conserved ... o-phenanthroline; P, untreated PTOX-expressing; PO, expressing PTOX and treated with o-phenanthroline; POF, expressing PTOX and treated with both o-phenanthroline and 125 lM FeSO4 (B) The upper ... addition of these compounds) The latter activity (approximately 65 nmolỈmin)1Ỉmg protein) 1) was calculated from the O2 consumption in the presence of KCN minus residual O2 consumption after n-propyl...
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