... additions [21, 22] Importantly, this template doesnot contain AREs that would interfere with HuR binding to the RNA (data not shown) DNA synthesis reactions carried outwith this T/P in the presence ... upon titration with unlabeled RT or HuR Furthermore, HuR did not affect the kinetics of HIV-1 reverse transcription in vitro Taken together, our results suggest that HuR doesnot impact HIV-1 ... to directly interact withthe RNase H domain of HIV-1 RT [9] To further define the structural determinants of the HuR-RT interaction and to elucidate the mechanism(s) by which HuR influences...
... cannot be addressed by a further extension of the present theory, by the framework of the agency theory and the theory of asymmetric information The question goes into the heart of the present theory, ... intermediaries when they assess the impact of shocks via the financial system on the real economy However, they notgo into the theoretical consequences In the next sections, we come up withthe rationale ... imperfection The mainstream theory of the firm evolved under the paradigm of the agency theory and the transaction costs theory as a theory of economic organization rather than as a theory of entrepreneurship...
... + mice, the pattern is virtually identical withthe exception of the lack of the PV spot in PV– ⁄ – mice (Fig 2A–C) This indicates that expression of fiber type specific MLC isoforms is not affected ... seen in SOL These findings further support the presence of distinct pathways regulating fiber specific isoforms and metabolic enzymes, on the one hand, and mitochondrial biogenesis, on the other Renewed ... membrane potential is maintained Nonetheless, the additional mitochondria in PV– ⁄ – TA not exactly match, with respect to the kinetics of Ca2+ uptake, to the situation present in WT TA, as both...
... the translation mixture with EDTA and was immunoprecipitated with an antiserum against the HA-epitope (data not shown), indicative of crosslinking to the released full-length form of SufIHA Notably, ... of the Tat signal peptide prohibits interaction with Ffh [5,34,35] Furthermore, the ÔSec-avoidanceÕ motif in the C-terminal region of the Tat signal peptide [36] might prevent rerouting via the ... deletion of tig doesnot affect the export of SufI which proceeds in vivo withthe slow kinetics that are characteristic for the Tat translocation process (Fig 4) In contrast, the export of SecB...
... and the integrity of the loxP sites in the floxed clone after Cre-mediated deletion The PCR across the loxP sites confirmed the presence of both loxP sites in the targeted clone (Fig 1B) Furthermore, ... analysis demonstrated that about 50% of the offspring are heterozygous for the full deletion allele The heterozygous mice were then intercrossed in an attempt to generate homozygous null mutants Animal ... was digested with BamHI and probed with probe The wild-type and the recombinant band are the expected size PCR with primer pairs Pr1 + Pr2 and Pr3 + Pr4 shows the correct placement of the two remaining...
... sites, tethering microtubules to the cell cortex, etc [45,52,53] In this scenario, we can imagine that the +TIPs complex is responsible for the elongation of the neural processes without the need ... to the absence of any band on the immunoblot stained with a commercial anti-MAP1b (Fig 4) and the strong evidence about the absence of MAP1b mRNA (Fig 5), leads us to conclude that MAP1b is not ... (Fig 6), and the other proteins tested (not shown) are present in CAD cells in normal amounts and with normal cellular distribution, suggesting that these proteins are not involved in the mechanism...
... mice, whereas the band intensity is about half of control (0.55 ± 0.09) in heterozygous mice, thus confirming the results obtained withthe cDNA To investigate whether disruption of the Tm7sf2 gene ... To test whether the mutation abolishes the expression of C14SR, RNA was extracted from liver and the cDNA was synthesized by RT-PCR using the forward and reverse primers that amplify the entire ... sequenced Comparison of the mouse genomic and the cDNA sequences revealed that the exon–intron organization of the mouse Tm7sf2 gene is highly similar to the homologous human gene [1] The gene spans ...
... experiment without incubation with antibody 218 9 ⁄ 90 In a similar experiment in whichthe order of the incubations with OG–HDLp and antibody 218 9 ⁄ 90 was reversed, the fluorescence of the cells ... However, the cells were first incubated for 30 with primary antibody 218 9 ⁄ 90, and then for 30 with OG–HDLp, or vice versa The degree of binding was compared to the degree of binding without the antibody ... incubations did not affect the size or the morphology of the cells, and thereby the amount of fluorescence The population with low fluorescence intensity was excluded from the analysis by defining...
... were sterilised without their knowledge or consent, which was not required They complain that the operations damaged their physical integrity, their right to found a family and that they suffered ... found they were unable to conceive after having caesareans Suspecting that they were sterilised without their knowledge during the operations, they sued the two Slovak hospitals concerned The Court ... pregnancy if the foetus showed symptoms of the same disease The Court concluded that the interference withthe applicants’ right to respect for their private and family life was disproportionate The Court...
... either goats or sheep occur, or both Addition NOT ^ Exclusion a query goats ^ sheep retrieves all records where goats occurs, unless sheep occurs in the same record Note: Be careful withtheNOT ... index The inverted file contains extracted search terms, together with links to the records from which they were extracted RECORD … 24: All is well that ends well … RECORD … 24: Much ado about nothing ... searches for the two words next to each other PLANT BREEDING there may be one word in between If such a field contains the record can be found by searching PLANT BREEDING The database...
... at the end of the article large 192-kb genome enables a large amount of foreign DNA to be incorporated without reducing the replication efficiency of the virus, which has been shown not to be the ... with hNIS-3 were used in separate PCRs, both with pCRII-hNIS-1 as the template The respective PCR products were then mixed and used as the templates in one reaction with hNIS-5 and hNIS3 as the ... expression cassette (SE-hNIS) containing the hNIS cDNA under the control of the VACV synthetic early promoter, by homologous recombination in infected cells The genotype of GLV-1h153 (Figure 1a) was...
... at the end of the article large 192-kb genome enables a large amount of foreign DNA to be incorporated without reducing the replication efficiency of the virus, which has been shown not to be the ... with hNIS-3 were used in separate PCRs, both with pCRII-hNIS-1 as the template The respective PCR products were then mixed and used as the templates in one reaction with hNIS-5 and hNIS3 as the ... expression cassette (SE-hNIS) containing the hNIS cDNA under the control of the VACV synthetic early promoter, by homologous recombination in infected cells The genotype of GLV-1h153 (Figure 1a) was...
... genes can alter the environmental microbiota Nevertheless, we ignore whether part of these alterations might remain over the long term Whereas antibiotics are degraded in nature, the genetic platforms ... Martinez, JL The role of natural environments in the evolution of resistance traits in pathogenic bacteria, Proc R Soc B 2009 276, 2 521- 2530 18 Hug, K Genetically modified organisms: the benefits outweigh ... doi:10.1186 /219 0-4715-23-1 Cite this article as: Taube F, et al.: The booklet „Genetically modified crops“, published from the German Research Foundation, doesnot meet the given claim – Discussion article Environmental...
... impoverishment They stand armed to the teeth, ready to destroy one another and their common civilization withthe devilish weapons which their science has placed in their hands There is change, ... level as their barbarian predecessors in their relations with one another Fearing and distrusting one another, they lock up a huge amount of their productive wealth in armaments, to their own ... science, our other scientific discoveries and inventions, and hundreds of other signs of progress? Two hundred years ago these things were unknown Had notthe world progressed far beyond the wildest...
... and neither are animal species 33 People say that the animals will be endangered in the forest A They said to be endangered in the forest B It is said that the animals will be endangered in the ... connected to the board by a flexible joint The sport is a hybrid between sailing and surfing The sail board might be considered the most minimalist version of the modern sailboat, withthe major ... forest C They are said that the animals will be endangered in the forest D It was said that the animals will be endangered in the forest 34 Even though he had no money, he decided to go traveling...
... atmosphere Aliquots of the sperm suspension were diluted with fresh medium to adequate concentration The aliquots of 30 ml were placed in pre-warmed slide chambers withthe depth of 20 mm The slide chambers ... counts in the epididymis by about 42% compared to the control (p < 0.05) Testicular sperm count was also decreased by the treatment of 17b-estradiol but it was not significantly different from the ... the epididymis, compared to the control (p < 0.05) The PHGPx expression in the epididymis was much higher by genistein than by 17β-estradiol (Fig 5) There were no significant differences in the...
... with nifedipine (60 mg/day) both for a cardiac study and for the present biological evaluation The second evaluation was carried out in the morning, hour after the last intake of nifedipine The ... ameliorated by nifedipine is consistent withthe hypothesis that VEGF signalling is impaired in SSc Our results also not support the implication of the sVEGFR-1 in the VEGF dysregulation, but more experimental ... to treatment with dihydropyridine-type calcium-channel blockers [17] The data reported herein confirm these results with a different design of the study (midterm effects) and extend the known beneficial...
... during the study period Infliximab (Centocor BV, Leiden, The Netherlands) was given as three intravenous infusions of mg/kg in accordance withthe recommended standard-treatment protocol, withthe ... protein in the synovia during therapy could be detected, but there was no correlation withthe clinical course of individual patients In the group of six patients who responded to therapy, three ... http://arthritis-research.com/content/10/2/R33 Figure study the therapeutic effects of systemic infliximab therapy on the synovial expression of other important pro-inflammatory molecules [38,47] Infliximab treatment in another small cohort...
... of the TSP-2 gene with respect to the morphology and cellularity of the annulus, the presence of vascular beds within the disc, and vascularity of the soft tissue margin of the disc that serves ... may therefore not participate in regression of neonatal vessels in the disc Morphology of the annulus in the TSP-2-null mouse Although there was no difference in the number of disc cells in the ... of expression of the TSP-2 gene, vascular ingrowth into the body of the disc did not occur The results of the quantitative assessment of the vascular bed in soft tissue along the disc margins...
... Apparently, the association of the CTGF -945GC SNP with disease is notthe same in all patient groups and categories, as has been noted in previous studies that could not always confirm the originally ... 10% increase in DN patients of the GG genotype frequency, i.e an increase to 31.9% in DN as compared to the21. 9% in the NA patients which was comparable withthe previously observed range of ... Blegdamsvej 9, DK - 210 0 Copenhagen, Denmark Authors’ contributions AD carried outthe genotyping assays, analyzed the data and wrote the manuscript TQN participated in the design of the study and...