ca2 influx via the l type ca2 channel voltage window in mouse aortic segments is modulated by nitric oxide
Báo cáo khoa học: The autophagic response to nutrient deprivation in the hl-1 cardiac myocyte is modulated by Bcl-2 and sarco⁄endoplasmic reticulum calcium stores ppt
... cell death linked to, but distinct from, apoptosis [9,10] Beclin 1, a class III phosphatidylinositol 3-kinaseinteracting protein [11], plays a role in promoting autophagy [12] Beclin contains ... flux, a lysosomal inhibitor cocktail consisting of the cell-permeable pepstatin A methyl ester (PepA; lgÆlL)1, inhibitor of cathepsin D), (2S,3S)-trans-epoxysuccinyl -l- leucylamido-3-methylbutane ... that in cancer cells, Beclin 1DBcl2BD, as well as other Beclin mutants lacking the ability to interact with Bcl-2, increased the percentage of cells containing numerous autophagosomes; these...
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Báo cáo khoa học: Androgen receptor function is modulated by the tissue-specific AR45 variant ppt
... °C in small aliquots Cell culture and transfections Cell lines were obtained from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany), except the PC-3 ⁄ AR cells, ... number of viable cells was determined after days in a Lumicount luminometer (Packard) using the Cell Titer-Glo assay (Promega) The two-hybrid assay was performed in CV-1 cells in the 96-well format ... androgen insensitivity have been reported [28] Finally, polymorphisms affecting the length of glutamine, proline and glycine repeats exist [28] They may lead to severe pathologies, as observed in Kennedy’s...
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Báo cáo khoa học: Proteolytic degradation of nitric oxide synthase isoforms by calpain is modulated by the expression levels of HSP90 potx
... correlated with the level of calpastatin present in the cytosol By quantification analysis of the immunoblots, it has been established that the amount of calpain retained by immobilized HSP90 is almost ... endothelial cells Am J Physiol Lung Cell Mol Physiol 278, 1204–1212 ´ Laine R & Ortiz de Montellano PR (1998) Neuronal nitric oxide synthase isoforms a and l are closely related calpain sensitive ... factors and mechanisms, including protein–protein interactions involved in allosteric regulation, scaffolding and trafficking [3,8–13] Of particular interest is the interaction with the chaperone...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... GL column (total volume, 24 mL) equilibrated in buffer A containing 50 mm NaCl using an FPLC system The flow rate was 100 lLÆmin)1 and the eluted proteins were collected in 500 lL fractions The ... Role of calpain in hypoxic inhibition of nitric oxide synthase activity in pulmonary endothelial cells Am J Physiol Lung Cell Mol Physiol 278, 1204–1212 24 Bellocq A, Doublier S, Suberville S, Perez ... previously to induce an increase in the level of [Ca2+ ]i and a correlated calpain activation [28] This increase in [Ca2+ ]i is more intense in aorta than in brain Under these conditions, in brain,...
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Báo cáo khoa học: "Immunohistochemistry of Voltage-Gated Calcium Channel α1B Subunit in Mouse Cerebellum" pptx
... of the normal mouse cerebellum The detailed analysis of subunit expression in serial sections of Purkinje cells provides important new data on the intracellular distribution of VGCC The present ... functional significance of the α1B channel type is not clear N -type currents are important in mediating neuropeptide release, since ω-conotoxin GVIA can block 50-70% of electrically evoked release ... structures Scale bar = 250㎛ Discussion The cerebellum is one of the few sites in the central nervous system where the pattern of intrinsic connections is known in considerable detail This knowledge has...
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Báo cáo khoa học: "The expression and localization of inhibin isotypes in mouse testis during postnatal development" pdf
... protein levels of the three isotypes of inhibin in the testis also increased The histological findings in the sexual maturation of developing mouse testis are consistent with those of a Fig Immunofluorescent ... Yujin Kim et al Temporal expression pattern of the inhibin isotypes α, βA and βB during the postnatal development of mouse testis The protein levels of the inhibin α, βA and βB isotypes in the ... Yujin Kim et al expression of inhibin isotypes might be observed in seminiferous tubules in mice during testicular development This study examined the level and cellular localization of inhibin...
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Báo cáo y học: "Tumor necrosis factor alpha-dependent aggrecan cleavage and release of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro" pptx
... perpendicular to the meniscus bottom surface Tissue disks mm in thickness were sliced including the original meniscal surface using a sterile scalpel blade, and four to five smaller explant disks ... was low or not detectable We were able to localize the NITEGE fragment in meniscal tissue by immunostaining in TNFα-treated explants, while it was almost non-detectable in control tissue This is ... (Figure 2) There was only one band detectable in the gels, which was missing or expressed at lower levels in controls, but strong in TNFα-stimulated cultures This band was not visible in gelatin zymograms...
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Báo cáo y học: "Failed surgical ligation of the proximal left subclavian artery during hybrid thoracic endovascular aortic repair successfully managed by percutaneous plug or coil occlusion: a report of 3 cases" ppsx
... perfusion of the false lumen in the remaining case Additionally, in all cases these radiological findings were associated with a gradual growth of the aneurismal sac or false lumen, stressing the importance ... the left subclavian artery; however, owing to surgical difficulties to clearly visualize and manipulate the deeply located proximal left subclavian artery, the ligation was incomplete in two cases ... dissection without clear false lumen dilatation subclavian artery via the cervicotomy The patient recovered well without neurological sequellae and the thoracic pain disappeared progressively...
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Báo cáo y học: "Modulation of epithelial sodium channel (ENaC) expression in mouse lung infected with Pseudomonas aeruginosa" pps
... PMN in BAL Total cells in BAL 100 % Cells in BAL Cells in BAL/ X 106 75 50 25 Day Day Day Day post-infection Day 14 Day 10.0 Cells in BAL/ X 105 Cells in BAL/ X 106 Day 14 Macrophages in BAL PMN ... Modulation of alpha-ENaC and alpha(1)-Na+-K+-ATPase by cAMP and dexamethasone in alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol 2001, 281 :L2 17 -L2 30 Finley N, Norlin A, Baines DL, ... has been developed in the mouse by the intratracheal instillation of P aeruginosa entrapped in agar beads In this model, the lungs of susceptible mouse strains develop severe lung infection with...
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Báo cáo sinh học: "Respiratory syncytial virus infection in Fischer 344 rats is attenuated by short interfering RNA against the RSV-NS1 gene" doc
... infiltration in lung is altered by siNS1 treatment RSV infection typically results in a migration of CD4+ T cells to the lung and interstitial infiltration of the sur- A ∗ control 40 B 20 siNS1 30 IL-4 ... tissues Intranasal administration of siNS1 reduced the number of CD4+ T cells in the lung but did not significantly alter the infiltration of CD8+ cells (Fig 4) The CD4+ T cell infiltration associated ... goblet cell hyperplasia in the bronchioles (upper photos) and fewer infiltrating inflammatory cells in the interstitial regions (lower photos) compared to controls (Fig 1A) The number of RSV plaque-forming...
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Investigation and characterization of splice variations of l type ca2+ channel, cav 1 3, in chick basilar papilla and rat cochlea hair cells iimplications in hearing
... ossicles via the eardrum move the membrane at the oval window, the motion in the oval window moves the fluid-filled cochlea and production of pressure waves within the cochlea displace the basilar ... preferentially localized to the cochlear outer hair cells (OHCs) while that of Cav1.3 IQfull (IQ-possessing) channels labeled the inner hair cells The preferential expression of Cav1.3IQD channels by ... rods and pillar cells along its length It consists of one row of inner hair cells, three rows of outer hair cells and several types of supporting cells namely the pillar cells, Deiters cells and...
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Calcium-sensing receptors regulate cardiomyocyte Ca2+ signaling via the sarcoplasmic reticulum-mitochondrion interface during hypoxia/reoxygenation pdf
... that CaCl2-activated CaR induces the increase of [Ca2+ ]i, but the origin of intracellular calcium remains unclear We examined [Ca2+ ]SR by Fluo-5N staining Fluo-5N is a low-affinity Ca2+ indicator ... role in the SR-mitochondrial inter-organelle Ca2+ signaling through the IP3Rs, which are also involved in apoptosis during H/Re Additional material Additional file CaR inducing apoptosis via the ... causes a rapid rise in [Ca2+ ]m [26] This effect has been detected in many cells types: HeLa cells, fibroblasts, endothelial and epithelial cells, cardiac and skeletal muscle cells, neurons and...
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Báo cáo Y học: A single charged surface residue modifies the activity of ikitoxin, a beta-type Na+ channel toxin from Parabuthus transvaalicus doc
... these cells is normally around )70 mV, and the voltage that typically activates the maximum, whole-cell Na+ conductance in these cells is between )10 and mV At the times marked by Ó FEBS 2002 the ... [10], using amphotericin B as the perforating agent and a single-electrode voltage- clamp amplifier (SEC-05LX; npi electronic, Tamm, Germany) in discontinuous voltage- clamp mode [11] The switching ... where Gly23 resides in birtoxin is solvent accessible (Fig 7) This is supported by the fact that the change alters the biological activity The surface potential calculation presentation also indicated...
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Báo cáo hóa học: " p53 represses human papillomavirus type 16 DNA replication via the viral E2 protein" docx
... shown to induce apoptosis in several cell lines, another explanation for these results could be that the cells expressing both proteins are dying or dead In order to rule out this possibility we ... transfected population (Fig 2B, column 2) Interestingly, E2m1 and E2m2 fail to induce apoptosis in these cells (columns and 4) In contrast, the remaining mutants induce apoptosis to levels comparable to ... apoptosis rises to around 20% of the transfected population (Fig 2A, column 3) All of the mutant E2 proteins examined in this experiment induce apoptosis in HeLa cells to around this level (Fig...
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Báo cáo hóa học: " Impact of Channel Estimation Errors on Multiuser Detection via the Replica Method" pot
... in [13, 14, 15] using the theory of large random matrices In this paper, we will apply the replica method to analyze the corresponding impact on optimal MUD, and then extend the results to linear ... denoting the channel estimation error by δgk (l) gk (l) − gk (l) , {δgk (l) } are jointly Gaussiandistributed and mutually independent for sufficiently large numbers of training symbols [16] Therefore, ... implicitly afh fecting the parameter q in the third term, the channel estimation error of the interfering users also results in the term of β∆2 ; an intuitive explanation for this is that, since...
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Single channel and whole cell electrophysiological characterizations of l type cav1 2 calcium channel splice variants relevance to cardiac and nervous system functions
... expressed in various cell types where they open upon sensing membrane depolarization to allow an influx of divalent Ca2+ ions into the cell The influx of Ca2+ ions is carefully controlled by fine-tuned ... CaV1.2 channels (Figure6) Single -channel recordings in combination with whole-cell electrophysiological recordings should allow a closer insight into the channel gating properties and allowing for ... result from alternative splicing The next paragraph will highlight the advantages of the cell attached mode of single -channel recordings in relation to the whole cell technique 1.5 Single-channel...
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Báo cáo y học: "A prospective observational study of the relationship of critical illness associated hyperglycaemia in medical ICU patients and subsequent development of type 2 diabetes"
... necrosis factor-alpha inhibits insulin-induced increase in endothelial nitric oxide synthase and reduces insulin receptor content and phosphorylation in human aortic endothelial cells Metabolism ... peripheral insulin resistance by inducing the release of stress hormones They also alter insulin receptor signalling [12-16] and create insulin resistance Due to these actions, glucose uptake in fat ... impaired glucose metabolism in the period following acute illness complicated with hyperglycaemia There was no tight glucose control policy in our department during the inclusion years Therefore, the...
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Developing Writing Skills in a Foreign Language via the Internet.doc
... similarly, similar to, like, likewise, is like, have in common, just as, compared to, same as, and resembles While contrast is indicated by the following: however, although, but, in contrast, unlike, ... sequencing (alternating pattern or point by point) With two subjects in mind, chunking separately presents all the points and details of the one subject Then the next section discusses and includes ... were initially assigned to work together e-mailed their finished product to the other team Once again, after the information from the other team has been received, each team will use listing in...
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The Mobile Radio Propagation Channel P1
... that the time delay involved in sending the message from control to the various transmitters in the system must be the same In other words, all the radio links in the system must be delay-equalised ... heavily built-up areas and within buildings The choice of frequency is therefore limited by the need to minimise the losses due to buildings while continuing to satisfy the other constraints mentioned ... this technique is widely implemented Naturally, the bandwidth occupied by a multiplexed link transmission is proportionally greater than an SCPC signal, but a 10 -channel multiplexed link connection...
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CHANNEL CATFISH CULTURE IN THE UNITED STATES BY
... Floating pellets are distributed by a feed blower mounted on a truck Floating pellets corn wheat soybean Feed amount is controlled by an electronic scale located in the truck cab Catfish are ... Fingerlings are stocked in fattening ponds during January to March Stocking density is 15,000 to 20,000/ha Catfish feed is purchased by the bag or delivered in bulk and stored in silos Floating ... years Catfish are retained in a holding net overnight to eliminate small catfish Fish are crowded for loading onto the transport truck A crew of can load 15,000 kg of fish in hour Catfish are...
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