biochemical elucidation of neutral lipid synthesis in microalgae
Báo cáo khoa học: Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in apah1Dmutant strain of Saccharomyces cerevisiae ppt
... evaluate the in uence of Arabidopsis and B napus PAHs in the metabolism of neutral lipids, we analyzed the TAG and PL content of yeast cells expressing recombinant PAHs (Fig 3B) After 48 h of induction, ... method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Jamal Z, Martin A, Gomez-Munoz A & Brindley DN ˜ (1991) Plasma ... as outlined above (Figs 4B, 5B and 6B) In conclusion, amino acid substitutions in the conserved C-LIP domain, including the HAD-like motif of AtPAH1 and BnPAH1A, resulted in the loss of PAP activity...
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Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx
... sequences show the coordinate of nucleotides and amino acids in corresponding lines The full-length cDNA encoding the putative GIH of P monodon was composed of 861 nucleotides containing a 5¢-UTR (93 ... Expression of Pem-GIH in the abdominal nerve cord of adult female shrimp at and h after incubating without ()) or with (+) GIH-dsRNA or GFP-dsRNA as indicated (C) Expression of Pem-GIH in the abdominal ... that in control shrimp The increase in the ratio of Vg to actin transcripts in the GIH-depleted background suggested that functional knockdown of Pem-GIH led to the induced expression of Vg in...
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Báo cáo hóa học: " Research Article Existence of Mild Solutions to Fractional Integrodifferential Equations of Neutral Type with Infinite Delay" pptx
... Advances in Difference Equations the solvability of the fractional-order functional integrodifferential equations of neutral type with in nite delay In the present paper, we will consider the following ... bounded subset of a seminormed linear space Y Kuratowski’s measure of noncompactness of B is defined as inf d > : B has a finite cover by sets of diameter ≤ d α B This measure of noncompactness satisfies ... subset of a Banach space X such that ∈ U, and let N be a continuous mapping of U into itself If the implication V convN V or V N V ∪ {0} ⇒ α V holds for every subset V of U, then N has a fixed point...
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Báo cáo khoa học:" Biochemical typing of pathological prion protein in aging cattle with BSE" ppt
... mg) using a) a core-binding antibody (mAB 6H4) b) an amino-terminal-binding antibody (mAb 12B2) and c) a carboxy-terminal-binding antibody (mAb SAF84) Molecular masses of a marker are shown in kDa ... characterize the biochemical PrPres phenotype in 33 out of the total of 37 aging BSE cases that had been identified since the beginning of the epidemic in Switzerland in 1990 With the exception of one ... Biochemical typing of different brain regions in H-type BSE Biochemical typing of different brain regions in Htype BSE Western blot analysis of the H-type BSE zebu (Charly-04) with a) a core-binding antibody...
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A functional genomics approach for elucidation of novel mechanisms involved in GnRH regulation of the gonadotropins
... Follistatin (FS) is a glycoprotein structurally unrelated to the activins and inhibins Due to its high binding affinity to activin, FS binds to and neutralizes the bioactivity of activin on the ... C domain is the DNA-binding domain, which consists of two zinc-finger-like motifs The D domain is a variable hinge The multifunctional C-terminal (domain E) is the ligand-dependent domain (LBD), ... Ubc4 over-expression increases the interaction of ERα with Sf-1or Pitx1 84 v 3.3 Calcineurin is involved in the GnRH activation of the αGSU gene promoter 86 3.3.1 Calcineurin catalytic subunit...
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Báo cáo khoa học: Quantitative modeling of triacylglycerol homeostasis in yeast – metabolic requirement for lipolysis to promote membrane lipid synthesis and cellular growth potx
... consequence of the sharing of a constant amount of LD between an increasing number of cells From our measurements, and in agreement with published data [16,17], we found that LD typically consists of ... storage of excess membrane material is possible by including a pool of membrane lipids in our model Ergosterol Phosphatidate Phosphatidylcholine Phosphatidylethanolamine Phosphatidylinositol ... pathways involved in the production of membrane lipids and MP, and their interaction with TAG mobilization, are illustrated in Fig By Fig Schematic representation of FA, neutral, and phospholipid...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... factor (ATF4) and of CCAAT ⁄ enhancer-binding protein homologous protein (CHOP) proteins downstream of eIF2a were observed (Fig 4A), indicating the initiation of ER stress-induced apoptosis Oleate ... periods of 3, 6, 12, 24 and 36 h, before staining of the accumulated neutral lipids with Nile red and analysis of the fluorescence intensity of individual cells by flow cytometry Cell fluorescence increased ... group, indicating increased incorporation of palmitate (16:0) into the TAG pool only in the presence of oleate [18] The above results raise two main questions: (a) what is the cause of TAG synthesis...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... the brain of a murine model of the syndrome [18,19] Interestingly, picolinate is reportedly able to prevent the neurotoxic effects of quinolinate in the rat central nervous system, suggesting that ... the bacterial protein [21,33] Interestingly, the same difficulty in stripping off the metallocofactor from the protein has been described for murine adenosine deaminase, a member of the amidohydrolase ... splicing of exons and of the ACMSD gene (Fig 2A); the presence of exon in ACMSD II causes a shift in the open reading frame, resulting in the occurrence of the first available start codon in exon...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... using 2014 crotonyl-CoA as a substrate [15] The gene encoding this bifunctional protein was named AUH (‘AU binding homolog of enoyl-CoA hydratase’) The RNAbinding activity of the human protein ... activity in a routine setting Indeed, we were able to show that AUH activity in fibroblasts can be determined by monitoring the formation of 3-hydroxyglutaryl-CoA The production of a small amount of ... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important role in leucine catabolism AUH was overproduced in E coli and characterized by measuring...
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Tài liệu Báo cáo khoa học: Treatment of neutral glycosphingolipid lysosomal storage diseases via inhibition of the ABC drug transporter, MDR1 Cyclosporin A can lower serum and liver globotriaosyl ceramide levels in the Fabry mouse model doc
... staining in Fabry mouse; (C, D) lung – epithelial cell staining increased in Fabry mouse; (E, F) brain microvascular endothelial staining in Fabry mouse Magnification ·16 no VT1 staining of normal ... staining is brown The section is counter stained with hematoxylin (B) In situ staining of renal VT1 bound in vivo VT1 (50 lg per mouse) injected i.p and bound within the kidney was immunostained ... activity in this cell line Metabolic labeling of neutral GSLs (including Gb3) within the Fabry cell line was prevented by CsA (Fig 1D), confirming MDR1 inhibition reduces de novo Gb3 synthesis Steady-state...
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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
... relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... consistently pointed to the formation of glutamate, proving unambiguously that the complex did indeed hydrolyze glutamine efficiently Kinetics of the glutaminase reaction The kinetics of glutamine hydrolysis ... acetylpyridine adenine dinucleotide (APAD) as cosubstrate [17] The glutaminase Pyridoxine biosynthesis in yeast (Eur J Biochem 271) 747 reaction was carried out in 0.3 mL of 50 mM Tris/HCl, pH 8.0, in...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured without and with iron as indicated (D) Perls’ staining of cytospins of ... impact in protein integrity, as indicated by an increase in protein-bound acrolein adducts at the cell surface, which point to acrolein-adducts as a reliable marker of lipid peroxidation also in ... with changes in intracellular lipid metabolism, we examined lipid content in iron-loaded HepG2 cells by Oil Red staining Faint lipid accumulation was observed in HepG2 cells grown in normal media...
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Tài liệu Báo cáo khoa học: A point mutation in the ATP synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction pptx
... contained 20 mM Tricine instead of succinic acid Assuming complete equilibration of the K+ during the h preincubation (see above), the value of the K+/valinomycin diffusion potential determined ... amounts of chromatophores and standard protein in the different lanes of a single gel were kept in the linear range of the luminol assay response Light-induced ATP synthesis Light-driven ATP synthesis ... injecting an acidified chromatophores suspension into a luminometer cuvette containing a basic solution and monitoring the ATP concentration continuously with luciferine/luciferase in the luminometer...
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Báo cáo khoa học: The pH dependence of kinetic isotope effects in monoamine oxidase A indicates stabilization of the neutral amine in the enzyme–substrate complex ppt
... 7.5), containing 0.5% (w ⁄ v) Triton X-100 and 0.2 mm kynuramine The activity was calculated by following the initial increase of A316 due to production of 4-hydroxyquinone and using an extinction ... was in the range 6.5– 9.5 The rate of enzymatic activity was determined by monitoring the initial linear increase in absorbance at 250 nm due to the production of benzaldehyde, employing an extinction ... significance of the deprotonated form Thus, we propose that binding of the substrate to the active site leads to a perturbation of the pKa, effectively increasing the concentration of the neutral amine...
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Báo cáo khóa học: A single mutation that causes phosphatidylglycerol deficiency impairs synthesis of photosystem II cores in Chlamydomonas reinhardtii pdf
... shown) This indicates the likelihood of a translational defect of D1 synthesis occuring in these strains To determine if translation initiation of the psbA mRNA was compromised, we inserted a ... observed in the WT strain Due to the control by epistasy of synthesis (CES) process [1], D1 and apoCP47 display concerted rates of synthesis In the absence of D1, the rate of synthesis of apoCP47 ... the hydrophobic part of the molecule [35] If this binding occurs at an early step in D1 synthesis, i.e during the process of the cotranslational insertion of the D1 protein into the thylakoid membrane,...
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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
... purication of recombinant enzymes One-hundred millilitres of LB (LuriaBertani) [59] containing 30 lgặmL of kanamycin (for EhPPDK 100 lgặmL of ampicillin [11]) was inoculated with bacteria harboring ... be very similar to that of the medium in which it is cultured (pH 6.5) [38]; thus, the cytosolic pH of amoebae living in the lumen of the intestine is uncertain The rate of enzyme activity would ... rates of activity in typical glycolytic pathways (with ATP-PFK instead of PPi-PFK and PYK instead of PPDK) At pH 6.0, the Vmax values of amoebal PGAM and PPDK showed a slight increase, those of...
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Báo cáo Y học: Inhibition of hyaluronan synthesis in Streptococcus equi FM100 by 4-methylumbelliferone doc
... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 32 Laemmli, U.K (1970) Cleavage of structural proteins during the assembly of the ... identifiable species In this regard, our most interesting result indicated that with increasing time of MU treatment there was a decrease in the proportion of smaller CL species and an increase in the larger ... activity of their membranes In order to examine whether possible metabolites of MU are involved in the inhibition of HA synthesis in intact cells, we performed some experiments using HPLC or ion-chromatography...
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Báo cáo Y học: Formation of conjugated D11 D13-double bonds by D12-linoleic acid (1,4)-acyl-lipid-desaturase in pomegranate seeds doc
... system of the different linolenic acid isomers is determined Plant oils containing conjugated linolenic acid derivatives are of commercial interest, since they are used as drying oils in paints ... cloned into yeast expression vectors under the control of the inducible GAL1 promoter and the encoded proteins were expressed in S cerevisiae strain INVSc1 In induced cultures of cells harboring ... between linoleic acid and c-linolenic acid, yeast cells harboring PuFADX were grown in the presence of an equimolar mixture of linoleic acid, a-linolenic acid and c-linolenic acid The resulting fatty...
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Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe pdf
... Molecular Biochemicals) In all cases the suppliers’ recommendations were followed in terms of incubation times and washing of the complexes Detection of neutral trehalase activity in gel Affinity ... shift might argue against the participation of a unique set of interactions involving association of Ntp1p molecules and could be taken to indicate a specific event occurring during activation by ... together, these results indicate that Ntp1p and Tps1p interact in vitro in S pombe, and that the nature of this association is specific and independent of the presence of the GST domain fused to Tps1p...
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To Cut or Not to Cut? That is the (Central Bank’s) Question In Search of the Neutral Interest Rate in Latin America pdf
... effective in finetuning the business cycle as periods of relaxing monetary policy (decreasing interest rate gaps) are followed by shrinking (negative) output gaps (and vice versa) 6 The estimated interest ... following system of equations: where is the nominal short-run (90-day paper) interest rate, the neutral nominal interest rate, stands for the rate of inflation, is the inflation target of the ... has maintained financial intermediation in Reais, it has created a system of unusually short duration financial contracts The legacy of indexation to the overnight interest rate has created institutional...
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