benefits of a leadership role
Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf
... demonstrated that Asp129 of NirFcould not be essential for any function similar to thatin Asp141 of Met8P.The idea of NirF being a dehydrogenase is appealingbecause of the presence of a putative ... understood. Anal-ysis of insertional mutagenesis and complementationwork in Pseudomonas aeruginosa, Pseudomonas fluores-cens, Paracoccus denitrificans and Pseudomonas stutzerihave shown that a set of ... 23 Chang CK (1994) Heme d1and other heme cofactorsfrom bacteria. Ciba Found Symp 180, 228–238.24 Van Spanning RJ, Wancell CW, De Boer T, HazelaarMJ, Anazawa H, Harms N, Oltmann LF &...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... mutationsMousumi Banerjee1, Hemalatha Balaram2and Padmanabhan Balaram11 Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India2 Molecular Biology and Genetics Unit, Jawaharlal ... Ravindra G & Balaram P (2005) Plasmodiumfalciparum triosephosphate isomerase: new insights intoan old enzyme. Pure Appl Chem 77, 281–289.20 Parthasarathy S, Ravindra G, Balaram H, Balaram ... inenzymes: a study of triosephosphate isomerase andcomparison with methyl glyoxalsynthase. Adv ProteinChem 66, 315–372.45 Gunasekaran K, Ramakrishnan C & Balaram P (1996)Disallowed Ramachandran...
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Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
... 5Â-gagagatttgctttgcgggttatggatctgc-3Â; mutation K20 1A, 5Â-gttatggatctgctaccgcggctccgatcctaaacg-3Â; mutation K201F, 5Â-ggttatggatctgctacttcgctccgatcctaaacgc-3Â; and mutation M45 3A, 5Â-ggacaactttgaatgggcggagggttatattgag-3Â. The ... sense strand are listed, as follows: mutation E19 0A, 5Â-caacgagcctagagcgatttgctttgagg-3Â; mutation E190Q, 5Â-caacgagcctagacagatttgctttgagg-3Â; mutation E19 4A, 5Â-gagagatttgctttgcgggttatggatctgc-3Â; ... Isorna P, Polaina J, Latorre-Garcı´ a L, Can˜ada FJ,Gonza´lez B & Sanz-Aparı´cio J (2007) Crystal structure of Paenibacillus polymyxa b-glucosidase B complexesreveal the molecular basis...
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Tài liệu Báo cáo khoa học: The most C-terminal tri-glycine segment within the polyglycine stretch of the pea Toc75 transit peptide plays a critical role for targeting the protein to the chloroplast outer envelope membrane ppt
... study.Name Sequence (residues 91–100)WT GGGAGGGGGGpolyAla *SA*AAAAA*AGG AAA*******GAG ****AAA***GGA *******AAAGAA ****AAAAAAAGA AAA****AAAAAG AAA*AAA***GGD *******DDDGGE *******EEEGGL *******LLLGGN ... tion apparatus, faces the stromal compartment. J BiolChem 273, 16583–16588.21 Gunasekaran K, Nagarajaram HA, Ramakrishnan C &Balaram P (1998) Stereochemical punctuation marks inprotein ... or glutamic acid also caused missorting of Toc75 to the stroma. Bycontrast, its replacement with repeats of asparagine, aspartic acid, serine,and proline did not largely affect correct targeting....
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... antibodieswere as follows: anti-porin from Calbiochem, anti-HA fromCovance BabCo, anti-mouse and anti-rabbit secondaryantibodies from Bio-Rad Laboratories and AmershamPharmacia Biotech, respectively. Antibodies ... fraction is designated as themitochondrial fraction.Immunochemical assays and antibodiesMitochondrial protein samples (between 50 and 100 lg)were separated by SDS/PAGE and BN/PAGE and trans-ferred ... s uspension wasused per assay. Oxygen consumption was measured polaro-graphically with a Clark oxyge n electrode in metabolicchamber with a water jacket maintained at 37 °C(Hansa-tech, Norfolk,...
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Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc
... 11–813.119. Wada, A. , Ogo, S., Watanabe, Y., Mukai, M., Kitagawa, T.,Jitsukawa, K., Masuda, H. & Einaga, H. (1999) Synthesis andcharacterization of novel alkylperoxo m ononuclear i ron(III)complexes ... J.N., Corina, D. & Akhtar, M. (1996) Themechanism o f the a cyl-carbon bond cleavage re action catalyzedby recombinant sterol 1 4a- demethylase of Candida albicans.(other names are: lanostero ... N-oxygenation of the tertiary arylamine at a rateless than half that of the wild-type-catalyzed reaction [142],so that reasonable interpretation o f the data seems d ifficult.Evidence from comparative...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... Institute of Physics, Academia Sinica, Taipei, Taiwan, ROC3 Department of Biochemistry, University of Minnesota College of Biological Sciences, St Paul, MN, USAStaphylococcal nuclease (SNase) is a ... folding pathway: structure-based analysis of staphylococcal nuclease. Proteins: Structure, Functionand Genetics 27, 171–183.15 Flanagan JM, Kataoka M, Fujisawa T & EngelmanDM (1993) Mutations ... Salmon testesDNA and some analytical grade chemicals such as EDTA,Tris ⁄ HCl, CaCl2, NaCl and mineral oil were obtained fromSigma (St Louis, MO, USA). Salmon testes DNA for theenzyme activity...
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Tài liệu Báo cáo khoa học: A possible role of mitochondria in the apoptotic-like programmed nuclear death of Tetrahymena thermophila Takashi Kobayashi and Hiroshi Endoh docx
... T. Kobayashi and H. Endoh5380 FEBS Journal 272 (2005) 53785387 ê 2005 FEBS rial large subunit rRNA (mtLSUrRNA) gene: mtLSU-3, 5Â-TACAACAGATAGGGACCAA-3Â; and mtLSU-4, 5Â-CCTCCTAAAAAGTAACGG-3Â. ... & Vaux DL(2000) Identification of DIABLO, a mammalian proteinthat promotes apoptosis by binding to and antagonizingIAP proteins. Cell 102, 43–53.7 Suzuki Y, Imai Y, Nakayama H, Takahashi ... (A) Fractionation PCR. A partial fragment of the mitochond-rial large subunit ribosomal RNA (23S rRNA) was amplified by PCR,using fraction samples that contained equal amounts of protein.Lane,...
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Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf
... the factthat H21 0A and H210S are as active as native API withVLK-MCA as a substrate (Table 1). This means thatTrp169 does not play a role as an electron-rich entity but as a large planar hydrophobic ... Shiraki, School of Materials Science,Japan Advanced Institute of Science and Technology, 1-1 Asahidai,Tatsunokuchi, Ishikawa, 923-1292, Japan.E-mail: kshiraki@jaist.ac.jpAbbreviations:API,Achromobacter ... Asp113 and His210, rather than a catalytictriad.ACKNOWLEDGEMENTWe are grateful to Dr. T. Yamazaki for NMR measurements, Y. Yagifor the amino acid analysis, and Y. Yoshimura for the sequence analysis.REFERENCES1....
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Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf
... mutant alkaline phosphatases. (A) Temperature dependence of kcat of TAB5 (d), mutants G26 1A (r), G26 1A/ Y26 9A (j)andE.coli (·) alkaline phosphatases attemperature range 5–25 °C. kcatvalues ... 1230–1238.22. Garen, A. & Levinthal, C. (1960) A fine structure genetic andchemical study of the enzyme alkaline phosphatase of E.coli.1.Purification and characterization of alkaline phosphatase.Biochim. ... higher value almost 2.5-fold higher thanthe native cold adapted enzyme (Table 1). The mutantG26 1A/ Y26 9A exhibits an E a almost the same as in the caseofthenativeenzyme(Table1).Thermal inactivation...
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The Costs and Financial Benefits of Green Buildings: A Report to California’s Sustainable Building Task Force pptx
... California Department of Water Resources Gary Estrada California Department of General Services, Office of Risk and Insurance Management Karen Finn California Department of Finance Doug Grandy California ... Program Sam Baldwin US Department of Energy Panama Bartholomy California Department of General Services, Division of the State Architect John Blue California Integrated Waste Management Board Bob ... renewable energy, such as the installation of over an acre of photovoltaic panels on the roof of the Franchise Tax Board Building in Rancho Cordova – which is the largest array on any state office...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... glycosyl-transferase elucidate catalysis in the alpha-amylase family. Nat.Struct. Biol. 6, 432–436.16. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K.H., Isogai, A. , Henrissat, B. & Dijkstra, ... circulans, whereas it may be mutated toasparagine without loss of activity in other family 18chitinases [28,30]. Preliminary results of a comparativestudy of available structures of family ... 2003 at four time points for each reaction (product formation waslinear for at least 20 min, at all substrate concentrations, atalmost all pH values and for all mutants). In this way kineticparameters...
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Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export pptx
... (5Â-ACGCGGATCCAGTCATAAACAGCGGTTGC-3Â, Bam HI site un derlined),87SufI-BamHI-rv (5 Â-ACGCGGATCCAACATCGTCGCCCTTCCA-3Â, BamHI site underlined) and SufIHA-XbaI+ClaI-rv (5Â-ACTGATCGATCTAGATTACGCATAGTCAGGAACATCGTATGGGTAGCCGCCTGGCGGTACCGGATTGACCAAC- ... a s a template and the primersRRTorA-SacI-fw (5Â-GCGCGGAGCTCAAGAAGGAAGAAAAATAATGAAC-3Â, SacI site underlined) andTorA/Lep2-BamHI-rv (5Â-GCATGGATCCCGCGCGCTTGATGTAATC-3Â, BamHI site underlined). ... stopcodons (TAG) that are suppressedwith (Tmd)Phe-tRNAsupare indicated. (B) In vitro tran slation of 57SufITAG8 an d 93SufITAG8. After translation, one hal f of eachsample was irradiated with...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... 5Â-CTAGACTCGAGCCTAATTTATATTTGCTCCTTGTGC-3Â. b-Actin primers weredesigned as follows: forward 5Â-CTACAATGAGCTGCGTGT-3Â and reverse 5Â-AAGGAAGGCTGGAAGAGT-3 ¢.Cell survival and apoptosis analysisFor viability ... theyeast two-hybrid assay and using a mammalian hybridsystem (Invitrogen, Carlsbad, CA) (EDA Wheeler &V Ayyavoo, unpublished data). A blast searchrevealed that the IMAGE clone, localized ... VBARP-mediated cellsurvivalCaspases, a family of cysteine acid proteases, are cen-tral regulators of apoptosis [12,13]. Caspases are rou-tinely used as a measure of apoptosis, in contrast...
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The Carbon and Global Warming Potential Impacts of Organic Farming: Does It Have a Significant Role in an Energy Constrained World? pptx
... may already offer efficiencies, an aspect that requires more study. Finally, reducing transport offers some additional, if smaller, potential for E and GHG gains (and again data for the Canadian ... survey, namely the Organic Agriculture Centre of Canada, the Organic Value Chain Roundtable and Agriculture and Agri-Food Canada. References and Notes 1. Canning, P.; Charles, C .A. ; Huang, S.; ... al. [27] is somewhat atypical of many current commercial organic crop production systems. An LCA modeling analysis of a Canada-wide conversion to organic canola, wheat, soybean and corn production...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25