b the ph of soft drinks
Tài liệu Báo cáo khóa học: Numerical calculations of the pH of maximal protein stability pptx
... reported to be stable in a range of pHs, then the optimum pH is taken to be the middle of the pH range The optimum pH in the numerical calculation is determined as pH at which the free energy of folding ... optimum pH (pH of optimal stability) is taken from BRENDA website [49] The calculated optimum pH (the pH of the minimum of free energy of folding) is given in the forth column The difference is the ... provides a broad range of pHs to be compared The origin of the optimum pH The position of the optimum pH depends on the amino acid composition and on the organization of the amino acids within the 3D...
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HYGIENIC PHYSIOLOGY WITH SPECIAL REFERENCE TO THE USE OF ALCOHOLIC DRINKS AND NARCOTICS BEING A REVISED EDITION OF THE FOURTEEN WEEKS IN HUMAN PHYSIOLOGY ppt
... forward, but are held erect by the strong muscles of the back The trunk is nicely balanced on the head of the thigh bones The great muscles of the thigh acting over the kneepan tend to bend the body ... producing the motions of the body, the muscles use the bones as levers We see an illustration of the _first class_ of levers in the movements of the head The back or front of the head is the weight ... [Illustration: _The Spine; the seven vertebræ of the neck, cervical; the twelve of the back, dorsal; the five of the loins, lumbar;_ a, _the sacrum, and_ b, _the coccyx, coming the nine "false vertebræ."_...
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báo cáo hóa học:" Unilateral or bilateral V-Y fasciocutaneous flaps for the coverage of soft tissue defects following total knee arthroplasty" ppt
... TKA the rehabilitation of the patient is delayed The earlier (according to the indications) this surgical technique is performed, the better for the rehabilitation schedule of the patient Mobilization ... adequate movement of the flaps without any tension at the periphery of the flap and the skin edges [24,25] In certain areas such as the frontal area of the knee where other types of skin or fasciocutaneous ... arteries supply blood to the skin on the anterior knee Although the skin blood feeding depends heavily on the terminal branches of the anterior anastomoses, there is a better blood supply originating...
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báo cáo hóa học:" Unilateral or bilateral V-Y fasciocutaneous flaps for the coverage of soft tissue defects following total knee arthroplasty" pot
... TKA the rehabilitation of the patient is delayed The earlier (according to the indications) this surgical technique is performed, the better for the rehabilitation schedule of the patient Mobilization ... adequate movement of the flaps without any tension at the periphery of the flap and the skin edges [24,25] In certain areas such as the frontal area of the knee where other types of skin or fasciocutaneous ... arteries supply blood to the skin on the anterior knee Although the skin blood feeding depends heavily on the terminal branches of the anterior anastomoses, there is a better blood supply originating...
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Báo cáo vật lý: "Influence of the Silica-to-Surfactant Ratio and the pH of Synthesis on the Characteristics of Mesoporous SBA-15" ppt
... morphological controls of SBA-15-type mesoporous silica are available in the literature.10,11 It is reported that the ratio of the silica source to the surfactant ratio and the pH of the synthesis ... layer The role of HCl in the synthesis process involves dehydrating the PEO segment and decreasing the solubility of the PEO block by the presence of Cl– ions in the aqueous solution.21 The increase ... from the data given in Table The samples were synthesised by varying the TEOS/TCP ratio but maintaining the pH value at 1.7 In each sample, the presence of micropores and mesopores was observed The...
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Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
... from pH 5.0 to 8.5 (50 mM citrate/phosphate buffer, pH 5.07.0; 50 mM phosphate buffer, pH 7.08.0; 50 mM Bicine buffer 7.08.5) The pH stability of Sfbgly50 was checked by incubation in the same buffers ... the deletion of the hydrogen bond between R97 and E399 is probably a major cause of the shift in the pKa of E399 Therefore, R97 facilitates the ionization of the catalytic nucleophile by stabilizing ... point is the average of four Sfbgly50 activity determinations using mM MUbglc as substrate The pH stability of the recombinant Sfbgly50 (n) was checked by incubating the enzyme in the same buers...
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Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... Ab, supershift group by KLF4 antibody (B) Recruitment of KLF4 to the binding element of the SR-BI promoter region The ChIP assay was used to detect the binding of KLF4 to the SR-BI promoter The ... whether KLF4 can bind to the SR-BI promoter Figure 3B shows the PCR product after the immunoprecipitation of the cross-linked chromatin with the KLF4 antibody As a specific control, purified rabbit ... enhancement of the expression of the tumour suppressor gene p53 [5] Because of the important roles of KLF4 on the above three cell types, we postulated the novel effect of KLF4 in atherogenesis...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... b- chitin was completely solubilized by LlChi18A, in both the absence and presence of LlCBP33A In the absence of LlCBP33A, the end-point of the reaction (i.e solubilization of all chitin) was reached ... that LlCBP33A has a preference for A B C Binding preferences for LlCBP33A Some family 33 CBPs bind to a broad selection of insoluble carbohydrates (e.g ChbB, which binds both a- and b- chitin ... the third demonstration of the accessory function of these proteins The effect of LlCBP33A on b- chitin degradation is of the same order of magnitude as the effect of CBP21 The effect on a-chitin...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... TFE, 10% TFE, 10% TFE, 10% TFE, 10% TFE, 10% TFE, pH pH pH pH Stefin B Y31 P79S mutant Stefin B E31 Stefin B Y31 pH pH pH pH pH pH 5 5 5 % of inhibition 63 80 41 66 58 62 32 ± ± ± ± ± ± ± 12 10 10 ... Analysis software, dichroweb [29,30] (http://www.cryst.bbk.ac.uk/cdweb/) Fibrillation assays The buffers used were 0.015 m acetate, 0.15 m NaCl, pH 5.0, NaCl ⁄ Pi pH 7.3 (or 0.01 m phosphate buffer pH ... corresponding peak from the Superdex 75 column, then, the monomer was incubated (see above) in the presence of Cu2+ and without Cu2+, at pH and pH The mobile phase was 0.01 m phosphate buffer, containing...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... assembly of the multisubunit complex in the inner mitochondrial membrane The bc1 subunits that are imported but not assembled into the multisubunit complex or subcomplexes thereof are probably ... mitochondrial membranes were analyzed by SDS/PAGE and Western blotting with antibodies to the subunits of the yeast bc1 complex indicated on the left side of the blots Ó FEBS 2004 Assembly of the yeast ... while the absence of the cytochrome b subcomplex (formed by Ó FEBS 2004 Assembly of the yeast cytochrome bc1 complex (Eur J Biochem 271) 1217 cytochrome b, subunit and subunit 8) hinders the insertion...
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Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx
... digestion of the genomic DNA with HindIII, homologous recombination and the excision of Bsr gene were confirmed by Southern hybridization using 0.9 kb DNA ()10.1 kb to )9.2 kb) as the probe (Fig 2) The ... kb and )6.5 kb); region II, promoter and upstream region of Ig -b gene ()2.7 kb to +0.05 kb; DHSs, )2.1 kb, )1.0 kb, and kb); region III, in the first intron of the Ig -b gene (+0.6 kb to +1.1 kb; ... size (given in kb) was determined by kHindIII Positions of the 1.3 kb, 2.7 kb, and 6.2 kb bands are shown in the right margin (C) Detection of the extra Ig -b mRNA by Northern hybridization Total...
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Tài liệu Báo cáo khoa học: Long-distance interactions between enhancers and promoters The case of the Abd-B domain of the Drosophila bithorax complex pdf
... with this possibility [22] If Fab-7 is replaced by either of these boundaries, communication will be blocked between the proximal enhancers and Abd -B The simplest interpretation of this result ... formed within the BX-C Conceivably, the normal function of the PTS in iab-7 is to help enhancers bypass the enhancer-blocking activity of the Fab)8 boundary without compromising other functions, ... region defined by the promoters of the two reporter genes (3¢ to one of them, Fig 4, bottom), the PTS is able to overcome the enhancer blocking effect of the boundary, and restricts the enhancer activity...
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Tài liệu An Account of the Proceedings on the Trial of Susan B. Anthony doc
... district (the said Susan B Anthony being then and there a person of the female sex,) as she, the said Susan B Anthony then and there well knew, contrary to the form of the statute of the United ... to the office of postmaster The case of Olive vs Ingraham (7 Modern Rep 263) was an action brought to try the title to an office On the death of the sexton of the parish of St Butolph, the place ... from the text of G & W "The verdict is to be the result of the deliberation of the jury upon all the evidence in the case The Court has no right to anticipate the verdict by an expression of opinion...
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Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... strongly polarize the carbonyl of the substrate The carbonyl carbon becomes very electrophilic and as the bond between the serine c-oxygen and the carbonyl carbon forms, the pKa of the Oc proton falls ... dependence of A328W Fig pH dependence for the turnover number (kcat) in the absence of excess substrate and for the turnover number (bkcat) in the presence of excess substrate, of the human BuChE ... number (bkcat) in the presence of excess substrate, of wild-type human BuChE over the pH range 4–8.5 From pH to 8.5 the assays were performed in 0.1 M sodium phosphate buffers From pH to 4.75 the...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... performed by using the Blast programs [26] at the server of the National Center for Biotechnology Information (Bethesda, Md., USA) Purification of b- 1,4-endogalactanase Culture fluid was collected by ... were stopped by the addition of 650 lL ethanol The precipitated substrate was removed by centrifugation and the supernatant was used to measure absorbance at 590 nm The pH optimum of GALA was ... degree of L-arabinose substitution by GALA results in different products Arabinogalactans with a low degree of L-arabinose substitution (potato, onion) as substrates result in the liberation of monomers,...
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Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt
... study of the effect of the antimicrobial peptide gramicidin S on the thermotropic phase behavior of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol lipid bilayer membranes Biochim ... However, the generally positive correlation between the effective hydrophobicity and amphiphilicity of the GS ring-size analogs and the magnitude of their perturbation of phospholipid bilayer membranes ... bilayer membranes and inhibit the growth of A laidlawii B These are the phospholipid bilayer/water partition coefficient, the localization and orientation of the peptide within the phospholipid bilayer,...
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Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf
... disappeared below pH 6.0 due to the protonation of His57 Ne2 These results not contradict the pH- activity profile of API shown in Fig Ion strength dependence of the pH- activity curve of API The pH- activity ... is pH units higher than that of His57 is the buried charge interaction with Asp113 The shielding effect of Asp113-His210 by Trp169 was supported by the independency of the ionic strength of the ... may be related to the solvent accessibility of the side-chain of His210 (Fig 3C) When the accessibility of the side-chain of His210 increased, the electrostatic interaction between His210 and...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
... the binding to lamin B [24] There has been no report about the effect of phosphorylation on the interaction of LBR and chromatin Therefore, a study on the cell cycle-dependent regulation of the ... Stimulation of the binding of LBR to chromatin by phosphorylation by a kinase(s) in a synthetic phase egg cytosol Chromatin binding of beads bearing GST–NK was increased by pretreatment with a synthetic ... indicate that LBR is indeed phosphorylated in the RS region by a synthetic phase cytosol, which stimulated the binding to chromatin Suppression of the binding of LBR to chromatin by phosphorylation...
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Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot
... evidence of the reformation of organized PLB if the pH of the low pH sample is returned to pH 7.5 by the addition of small amounts of KOH In contrast, the turbid PLB suspensions rapidly clarify and become ... the absorption maximum of the photoconverted enzyme occurring under these conditions to be inhibited by ATP and by the protein phosphatase inhibitor NaF On the basis of this study, and subsequent ... with the bA-aA-bB binding domain known as the Rossmann fold [29] The detailed organization of the NADPH-binding pocket has yet to be established for POR but it has been determined in other members...
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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt
... Chlamydomonas GBSSI in the same set of experiments Because of this high activity, we were able to double the amount of polysaccharide by a 24 h incubation of purified starch granules in the presence of 3.2 ... at the surface of the granule The apparent polarized growth of the granules is consistent with either an anisotropic distribution of GBSSI or a filling of the amorphous regions of the granule leading ... described by Sambrook et al [30] before hybridization with the specific nucleotide probe From a total of 16 positives clones, three were selected for further analysis because of their strong hybridization...
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