... Choose the roles to suit the task Moving group members 4 111 4 Use language points to set up groups: • • • • • • • I’m a 1! Are you a 1? Yes, I am Oh, Good! No, I’m a OK, bye! Sorry, I have ... beautiful, fantastic, great! Tell the students who is who • Animals – Are you a dog? No I’m a cat! 11 g r e ta ta sf ita cn e ita fu lub w n rd fe luo 4’s! The teacher is active: How? What does the...
... Humphries 19 77; Hartman 19 79; Barrett 19 84; Lewis 19 85) In contrast to Brenner and Ramas's argument that the family wage was in the interests of both male Interaction of class and gender 12 1 Particular ... materialism towards sociological materialism, see Wright, Levine and Sober (19 92: ch 5) Interaction of class and gender 11 9 research and theory construction Five forms of possible class/gender interconnections ... For example, a number of contributors to the ``domestic labor debate'' of the 19 70s (e.g Secombe 19 74; Gardiner 19 75) argued that the subordination of women is rooted in the sexual division of...
... GST-ExoS(S 419 I) GST-ExoS(Q420A) 10 GST-ExoS(G421A) 11 GST-ExoS(L422A) 12 GST-ExoS(L423A) 13 GST-ExoS(D424A) 14 GST-ExoS(A425K) 15 GST-ExoS(L426A) 16 GST-ExoS(D427A) 17 GST-ExoS(L428A) 18 GST-ExoS(LD426–427AA) ... Amersham S 419 QGLLDALDL428 M 419 AAAA428 S 419 QGLLDAAAA428 S 419 QGLLAAAAA428 S 419 QGLLAALAL428 I 419 QGLLDALDL428 S 419 AGLLDALDL428 S 419 QALLDALDL428 S 419 QGALDALDL428 S 419 QGLADALDL428 S 419 QGLLAALDL428 S 419 QGLLDKLDL428 ... 8, GST-ExoS(S 419 I); lane 9, GST-ExoS(Q420A); lane 10 , GST-ExoS(G421A); lane 11 , GST-ExoS(L422A); lane 12 , GST-ExoS(L423A); lane 13 , GST-ExoS(D424A); lane 14 , GST-ExoS(A425K); lane 15 , GST-ExoS(L426A);...
... headgroup and the integrity of the enzyme 10 11 12 13 14 15 16 Acknowledgements The work was supported by a Medical Research Council Fellowship to B.M 17 References Nett, J.H., Hunte, C & Trumpower, ... cytochrome c1, and Fe-S protein subunits at the ubihydroquinone oxidation site of the bc1 complex of Rhodobacter capsulatus Biochemistry 37, 810 5 – 811 4 Humphrey, W., Dalke, A & Schulten, K (19 96) VMD ... G167E ox, spectrum of aerobic mutant cells Ó FEBS 2004 Effect of G167E mutation in the yeast bc1 complex (Eur J Biochem 2 71) 12 95 Fig Steady-state level of the iron–sulfur protein (ISP) in G167E...
... KU532 KU 616 KU 617 KU 718 KU 719 KU1233 KU1234 KU1235 KU1236 KU1237 KU1009 KU1 010 KU1 011 KU1 012 KU 113 0 KU 113 1 KU 113 2 KU 113 3 fragments of pIB1 ⁄ (construct of PEX1 in pET21d) to plasmid pIB1 ⁄ A SauI ... were KU1009 ⁄ KU1 010 for Pex1p-ProtA and KU1 011 ⁄ KU1 012 for Pex6p-ProtA Constructs with Pex1pN(aa1– 412 )-ProtA, Pex1pN + D1(aa1–6 81) -ProtA, Pex6pN(aa1–428)-ProtA and Pex6pN + D1(aa1– 716 )-ProtA ... Pex6pN(aa1–428)-ProtA and Pex6pN + D1(aa1– 716 )-ProtA were amplified using primers KU 113 2 ⁄ KU1 010 , KU 113 3 ⁄ KU1 010 , KU 113 0 ⁄ KU1 012 and KU 113 1 ⁄ KU1 012 , respectively All point mutations generated were confirmed by...
... Campbell KP (19 95) SH3 domain-mediated interaction of dystroglycan and Grb2 J Biol Chem 270, 11 711 – 11 714 Winder SJ (20 01) The complexities of dystroglycan Trends Biochem Sci 26, 11 8 12 4 Ervasti ... 23, 14 56 14 71 10 Durbeej M, Henry MD & Campbell KP (19 98) Dystroglycan in development and disease Curr Opin Cell Biol 10 , 594–6 01 4942 11 Brancaccio A, Schulthess T, Gesemann M & Engel J (19 95) ... Phe 718 fi Ala were lysed with NaCl ⁄ Pi containing 1% Triton X -10 0 and protease inhibitors, and centrifuged at 16 000 g for 10 at °C with an Eppendorf 5804R centrifuge, rotor type F45-30 -11 (Hamburg,...