... 8, 3809–3 819 Lee WS, Wells RG, Sabbag RV, Mohandas TK & Hediger MA (199 3) Cloning and chromosomal localization of a human kidney cDNA involved in cystine, dibasic, and neutral aminoacid transport ... of enzymes Biochim Biophys Acta 154 6, 1–20 Wells RG & Hediger MA (199 2) Cloning of a rat kidney cDNA that stimulates dibasic and neutral aminoacid transport and has sequence similarity to glucosidases ... Heteromeric aminoacid transporters: biochemistry, genetics, and physiology Am J Physiol Renal Physiol 281, 995 101 8 Broer S & Wagner CA (2002) Structure–function relationships of heterodimeric amino acid...
... an elution gradient of 0 10 100% Buffer A, 10 20 0–27% Buffer B, 20–25 27% Buffer B, 25–58 27–42.5% Buffer B, and 58–63 42.5 100 % Buffer B Buffer A is 0.1% trifluoroacetic acidand Buffer B is ... Trp14 and Thr15 The observation of two small 3JHN-Ha coupling constants for residues Asn11 and Ser12 and the dNN(i,i + 2), daN(i,i + 2) [Ser12(CaH)–Trp14(NH) and Asn11(CaH)–Phe13(NH)] and daN(i,i ... pointing out that the l -amino acid to d -amino acid epimerization seems to be complete for conopeptides, whereas both isoforms coexist in defensin-like peptidesand natriuretic peptides from the Australian...
... N 210 is an active-site residue The mutant His-CoaB proteins N210A, N210E, N210H, N210K and N210Q formed dimers as had also been observed for the wt enzyme ([5]; Fig 4) Residues R206, N 210 and ... American Elsevier, New York Lynen, F (196 3–70) The Pathway from ‘Activated Acetic Acid to the Terpenes and Fatty Acids in Nobel Lectures: Physiology or Medicine, pp 103 –138, American Elsevier, New ... His-CoaB N210K and copurification of the 4¢-phosphopantothenoylCMP intermediate His-CoaB proteins (His-CoaB and His-CoaB N210K) were purified from E coli cell extracts by Ni-nitrilotriacetic acid and...
... Structure, Function and Bioinformatics 56, 271–277 Gill SC & von Hippel H (198 9) Calculation of protein extinction coefficients from aminoacid sequence data Anal Biochem 181, 319 326 FEBS Journal ... W140O and K133A] The curves of W140A and W140O are nearly linear in terms of intensity All protein concentrations were mgÆmL)1 of the wild-type protein and K133A and E142O were 88.45, 84.70, and ... stability identification and the role of key acidic aminoacid residues in staphylococcal nuclease unfolding FEBS Journal 272, 3967–3974 Chen HM, Markin VS & Tsong TY (199 2) pH induced folding...
... stressed and the relaxed conformations [15] The network involves the side chains of the amino acids in positions 53 and 56 in a helix B, 186 in b strand 3A, and position 334 in b strand 5A (Fig ... b strand 6A and the backbone of Asn171 in the a helix F/b strand 3A loop Note: SWISSPDB VIEWER uses the same signature for a helices and the short 310- helix found in the a helix F/b strand 3A ... number of substitutions in the shutter region and b strand 5A of PAI-1 and studied their effect on the transition to latent and substrate forms and on the stabilizing effect of vitronectin, a...
... (197 4) Serum proteins and plasma free amino acids in severe malnutrition Am J Clin Nutr 27, 733–742 Gietzen DW (199 3) Neural mechanisms in the responses to aminoacid deficiency J Nutr 123, 610 625 ... 40, 91 109 10 Iraqui I, Vissers S, Andre B & Urrestarazu A (199 9) Transcriptional induction by aromatic amino acids in Saccharomyces cerevisiae Mol Cell Biol 19, 3360–3371 11 Hinnebusch AG (198 8) ... to aminoacid availability has been studied extensively in yeast [8] The GCN2 and TOR kinases sense the intracellular concentration of amino acids In addition, yeast cells possess an amino acid...
... Integral Amino Length 5¢-UTR CDS 3¢-UTR Length acids Psy1 32 656 51 173 236 193 181 942 120 423 313 518 689 4527 Psy2 112 710 51 173 236 193 199 153 107 710 273 227 398 3542 a Protein Exons and were ... whiteflower locus Plant Cell 18, 194 7 196 0 Ray J, Bird C, Maunders M, Grierson D & Schuch W (198 7) Sequence of pTOM5, a ripening related cDNA from tomato Nucleic Acids Res 15, 105 87 Bartley GE, Viitanen ... yellow and the fruit is red? G Giorio et al Fig Sequence alignment of PSY1 and PSY2 protein derived from analysis with The predicted protein sequences were 412 and 438 amino acids for PSY1 and PSY2,...
... as < 5% Uptake of L-[3H]proline (100 lM) was measured at pH 6.5 for 10 in the presence of unlabeled amino acids and derivatives at a fixed concentration of 10 mM and are given as percentage inhibition ... N (199 2) Cloning and expression of a glycine transporter from mouse brain FEBS Lett 305, 110 114 16 Mackenzie, B., Loo, D.D., Fei, Y., Liu, W.J., Ganapathy, V., Leibach, F.H & Wright, E.M (199 6) ... set of amino acids and derivatives divided into different categories with differences in (a) side chain size, (b) backbone length, (c) O- and N-methyl substitutions, (d) stereochemistry and (e)...
... with H121 and K9, and of G129 with K 110 and K133 Other charged amino acids such as D73, D83 and E101 reinforce the interactions of E75 and E129 ˚ (OE1 ⁄ OE2)– are K 110 (Nz)+ (¼ 6.29 A) and K133 ... namely helix (V99 to Q106) and helix (E122 to K134) This finding implies that E129 and E101 form another local stable area through interactions with K 110 and K133 (Fig 4) and is in agreement with ... Local stability and key acidic amino acids in staphylococcal nuclease by the interactions of E75 with H121 and K97 It is believed that a small number of key aminoacid segments play major...
... three-dimensional structures of a -amino acids and those of b -amino acids and posed the following questions Which structures of b -amino acids overlap the canonical structures of a -amino acids? Subsequently, ... b -amino acid [19] , whereas the introduction of two adjacent b -amino acids induced a 10- membered hydrogen-bonded turn (C10) analogous to the b-turn made of a -amino acids [20] This propensity of b -amino ... representation of a-, b2- and b3 -amino acids with the torsion angles /, h and w For the b2 and b3 -amino acid substituted-SP analogues, the methyl side chain of b-HAla exhibits the same orientation as the...
... Purification and properties of D-aminoacylase of Streptomyces olivaceus Agric Bio Chem 42, 107 113 Ó FEBS 2002 19 Tsai, Y.C., Tseng, C.P., Hsiao, K.M & Chen, L.Y (198 8) Production and purification of D-aminoacylase ... T & Fukagawa, Y (198 0) Deacetylation of PS-5, a new beta-lactam compound II Separation and purification of L -amino acid acylase and D -amino acid acylase from Pseudomonas sp 115 8 J Antibiotic 33, ... of free amino acids andaminoacid residues in proteins by radiolysis and by metal-catalyzed reactions Ann Rev Biochem 62, 797–821 53 Stadtman, E.R (199 2) Protein oxidation and aging Science 257,...
... or dCMP showed that discrimination between CMP and UMP is achieved by Ser36, Arg 110 and Asp132, which form hydrogen bonds with the amino group and the N3 atom of the cytosine (Fig 1) This study ... Results Overproduction and molecular characterization of the modied variants of E coli CMP kinase The wild-type and various modied forms (S36A, R110M, D132A, D132H, D132N, D132S and R188M) of E coli ... M-D (199 0a) Mechanism of adenylate kinase Critical evaluation of the X-ray model and assignment of the AMP site Biochemistry 29, 109 5 6109 64 19 Tickle IJ, Laskowski RA & Moss DS (199 8) Rfree and...
... r11c (r11c N); middle panel, a mixture of two synthetic variants, r11c (r11c S) and [L-Leu42]r11c; bottom panel, a mixture of natural and synthetic r11c (r11c N and r11c S) Elutions were carried ... to the d version of the peptides simply as r11b and r11c, and the all-l versions as [l-Phe44]r11b and [l-Leu42]r11c Increased biological potency of D -amino- acidcontaining peptides A whole animal ... (presence of D -amino acid unlikely) C radiatus R11.3 C betulinus Bt11.1 C episcopatus Ep11.1 Group II C C betulinus BtX ⁄ BtX C virgo ViTx ⁄ ViTx C C C C Em11 .10 Fi11 .11 Ep11.12 Sx11.2 4184 emaciatus...
... loading onto the gel 2, 10and 100 lg of membrane proteins for UGT2B4, UGT2B4F33L and UGT2B4F33Y, respectively, and 12, 15and15 lg of membrane proteins for UGT2B7, UGT2B7Y33L and UGT2B7Y33F, respectively ... Mackenzie PI & Miners JO (199 7) The regioand stereo-selectivity of C19 and C21 hydroxysteroid glucuronidation by UGT2B7 and UGT2B11 Arch Biochem Biophys 341, 207– 211 13 Villeneuve L, Girard H, Fortier ... UGT2B7Y33F and UGT2B4 and 200 lg for UGT2B4F33Y and UGT2B4F33L in 100 mm Tris ⁄ HCl buffer (pH 7.4), 10 mm MgCl2 containing 0.02 mm UDP-glucuronic acidand 0.1 lCi UDP-[U-14C]glucuronic acid The...
... Ministry of Science and Technology (CICYT project PPQ2001 -190 8), and the Academy of Finland (projects 52 311 and 202409) The authors thank J M Cregg and C Gancedo for useful comments, and O Cos for ... C (2000) Carbohydrate and energy-yielding metabolism in non-conventional yeasts FEMS Microbiol Rev 24, 507–529 12 Jones, E.W & Fink, G.R (198 2) Regulation of aminoacidand nucleotide biosynthesis ... (199 8) 13C-NMR, MS and metabolic flux balancing in biotechnology research Q Rev Biophys 31, 41 106 Szyperski, T (199 5) Biosynthetically directed fractional 13C-labeling of proteinogenic amino acids...
... discrepancies Lys289 and Arg294 were found to be glutamic acidand alanine, respectively The codon for Val292 was found to be GUG and not GUC as published originally [19] Temperature and pH dependency ... shading, a second dimer with green and purple shading and a third dimer with blue and yellow shading Red atoms indicate amino acids that dier among B caldolyticus and B subtilis PRibPP synthases ... tted to Eqn and the following values were obtained: KATP 310 110 lM, KRib5P 530 140 lM and Vmax 440 69 lmolặmin)1ặmg protein)1 Assay of enzyme activity in the presence of a variety of nucleotides...
... Corresponding amino acids of human CYP11B2 and CYP11B1 as well as mouse and rat CYP11B1 at the positions selected for mutagenesis Position Human CYP11B2 Human CYP11B1 Mouse and rat CYP11B1 112 147 152 ... contribute to efficient CYP11B1 and CYP11B2 wild-type activity In addition, it is known from the crystal structures of CYP101, CYP108 and CYP102 that the N-terminal region encodes an amino- acid sequence ... substitutions at positions 147 and 152 (double mutant D147E/K152N), and 112 , 147 and 152 (triple mutant I112P/D147E/K152N) gave rise to cortisolproducing activity increases of 1.6-fold and 2.5-fold, respectively,...