amplified fragment length polymorphism represents another type of vntr that is smaller than the vntrs used for rflp
báo cáo khoa học: " Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism" pps
... results Another drawback was that the sequencing of the genome of N benthamiana, the experimental plant used in this study, is not yet complete Many cDNA fragments of N benthamiana identified by the ... drawbacks of this technique is that each banding from the AFLP reaction could comprise more than one cDNA fragment Therefore, it is important to confirm differential expression of the targets ... resuspend in 10 μl of deionized water to elute the mRNA cDNA synthesis For the first-strand cDNA synthesis, the 20-μl reaction containing 750 ng of mRNA, 30 pmole of Oligo (dT)40, 50 mM Tris-HCl pH 8.3,...
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Báo cáo khoa học: " Molecular fingerprinting of clinical isolates of Mycobacterium bovis and Mycobacterium tuberculosis from India by restriction fragment length polymorphism (RFLP)" pdf
... part of India The results of IS6 110 RFLP patterns of M tuberculosis obtained in our study therefore indicate that, this approach could be used successfully for discriminating clinical Indian isolates ... that consisted of pvuII fragments This pattern was found in 27 of 28 strains Molecular fingerprinting of M bovis and M tuberculosis by RFLP 333 Table Distribution of IS 6110 DNA fingerprint types ... 2) that consisted of single pvuII fragment of 1.9 kb To increase the accuracy of strain classification, we also used IS1 081 fingerprinting for M tuberculosis and M.bovis RFLP with IS1 081 probe...
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Báo cáo khoa học: " IS900 Restriction Fragment Length Polymorphism (RFLP) Analysis of Mycobacterium avium subsp. paratuberculosis Isolates from Goats and Cattle in Norway" pdf
... (1999) standardised the RFLP typing and nomenclature of the RFLP types, enabling a comparison of isolates from different parts of the world The aim of the present study was to investigate the genotypic ... (n=1) Discussion In the present study, all M a paratuberculosis isolates from cattle and 84% of the isolates from goats in Norway were of the B-C1 RFLP Table IS9 00 restriction fragment length polymorphism ... differences in the RFLP patterns were found for these types, and the difference was usually the absence or gain of one band Except for B-C5 and N-C20, only one isolate of each RFLP type was found The two...
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Báo cáo khoa học: "Oak chloroplast-DNA polymorphisms detected by restriction fragment length polymorphism (RFLP)" doc
... not show polymorphisms with the restriction enzymes used The polymorphism detected by the P6 Petunia probe with BglII had been described earlier (Kreike et al, 1991) The other polymorphisms are ... follow the inheritance of the most con- servative DNA sequences of plants cpDNA polymorphisms could provide information on evolutionary distances among oak species, the origins of populations and the ... reported to date In the only in the Q petraea samIn the latter two cases, howples (fig E) ever, the hybridization signal of the polymorphic fragment was weaker than that of the constant ones was...
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Báo cáo kỹ thuật di truyền kỹ thuật aflp (amplified fragments length polymorphism)
... 1990, công ty Keygene phát triển kỹ thuật AFLP ( Amplified Fragments Length Polymorphism) AFLP kết hợp RFLP (Restriction Fragments Length Polymorphism) PCR( polymerase chain reaction) đa hình đọan ... http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechAFLP shtml http://doc.edu.vn/tai-lieu/de-tai-chi-thi-phan-tu-ky-thuat-aflp -amplified- fragment- lengthpolymorphisms-61035/ http://idoc.vn/tai-lieu/chi-thi-phan-tu-ky-thuat-aflp.html http://tailieu.vn/doc/ky-thuat-aflp-348752.html ... phẩm AFLP: Sau PCR thể tích formamide dye thêm vào phản ứng, mẫu biến tính nhiệt 95 0C phút đặt vào đá Sản phẩm AFLP điện di gel polyaryamide biến tính(19 acryamide: bisacrryamide; 7,5 Murea; 0,5...
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báo cáo kỹ thuật di truyền- kỹ thuật aflp (amplified fragments length polymorphism)
... 1990, công ty Keygene phát triển kỹ thuật AFLP ( Amplified Fragments Length Polymorphism) AFLP kết hợp RFLP (Restriction Fragments Length Polymorphism) PCR( polymerase chain reaction) đa hình đọan ... http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechAFLP.shtml http://doc.edu.vn/tai-lieu/de-tai-chi-thi-phan-tu-ky-thuat-aflp -amplified- fragment- lengthpolymorphisms-61035/ http://idoc.vn/tai-lieu/chi-thi-phan-tu-ky-thuat-aflp.html http://tailieu.vn/doc/ky-thuat-aflp-348752.html ... phẩm AFLP: Sau PCR thể tích formamide dye thêm vào phản ứng, mẫu biến tính nhiệt 95 0C phút đặt vào đá Sản phẩm AFLP điện di gel polyaryamide biến tính(19 acryamide: bisacrryamide; 7,5 Murea; 0,5...
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Bài báo cáo môn di truyền phân tử kỹ thuật AFLP (amplified fragments length polymorphism)
... thuật AFLP (amplified fragment length polymorphism) , phương pháp có hiệu để nhân dòng giải trình tự marker liên kết để xác định đặc điểm chúng AFLP nhanh, đơn giản không phức tạp RFLP khảo sát toàn ... DNA/DNA hybrybization: RFLP minysatellite RFLP thường có hệ di truyền Dominant - Genomic base: Thí dụ SNP dùng để tách vùng đột biến - Protein base proteomic: marker chia kiểu + Isozyme enzyme marker ... AFLP không phức tạp RFLP khảo sát toàn gen - Có tính lặp lại cao RAPD cần sử dụng lượng nhỏ DNA ban đầu (2.5pg-25ng) - Khuếch đại có chọn lọc lượng lớn đoạn DNA đa hình (polymorphism) phản ứng PCR,...
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Báo cáo khoa học: The phosphatidylethanolamine level of yeast mitochondria is affected by the mitochondrial components Oxa1p and Yme1p ppt
... deletion of OXA1 decreased the rate of formation of all aminoglycerophospholipids The rate of PtdSer synthesis decreased to 80%, the rate of PtdEtn formation to 70% and that of PtdCho synthesis to ... affected First, is the supply of PtdSer to the mitochondria as a precursor for PtdEtn formation by Psd1p This process includes synthesis of PtdSer in the endoplasmic reticulum by the PtdSer synthase ... in the formation of the three aminoglycerophospholipids within the selected timeframe, which enabled us to determine the rate of formation, i.e the incorporation of radiolabel per period, for...
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Báo cáo khoa học: The GxxxG motif of the transmembrane domain of subunit e is involved in the dimerization/oligomerization of the yeast ATP synthase complex in the mitochondrial membrane doc
... from the top of the native gel to the position of the monomeric form of the enzyme From these data, it was concluded that the mutation e19A altered the relationship between subunit e and the other ... devoid of Cys28 (not shown), thus indicating the involvement of Cys28 in the formation of the adduct The 21.4-kDa band corresponded to a homodimer of subunit e resulting from the formation of a disulfide ... resulted from the incubation of wild -type mitochondria with CuCl2, was found in the oligomeric form of the ATP synthase but not in the dimeric form The subunit e and the dimer of subunit e of NEM-...
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Semantics Semantics is the study of meaning that is used to understand human expression through language.
... by the action In this case a ball Agent & theme The boy cut his hair with the scissors In this example, we can notice that there is a tool that helps the Agent to perform the action That is the ... Source Goal The boy kicked a ball In that sentence, the person who executes the action is called AGENT The agent in that sentence is the boy The THEME is the object or person that is directly ... in the context of “flies” Another fact dealing with this type is that “a small elephant” for example, is much bigger than “a big mouse” a Implicitly gradable pairs Another characteristic of...
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Báo cáo toán học: "The characteristic polynomial of a graph is reconstructible from the characteristic polynomials of its vertex-deleted subgraphs and their complements" doc
... this time H is also Hamiltonian.” A different problem than that proved by Theorem 3.1 is the question of whether PG (x) is reconstructible from the two decks P(G), P(G) Unlike the condition of the ... Moreover, the characteristic polynomials of the pairs G7 , H7 , G11 , H12 and G12 , H11 differ only in their respective coefficients of x The list of the characteristic polynomials of the vertex ... vertex-deleted subgraphs is the most general case of problems of this type There are counter-examples to the question of whether a graph invariant I(G) is reconstructible from the collection { I(Gu),...
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Báo cáo y học: " Effectiveness of an intercultural module added to the treatment guidelines for Moroccan and Turkish patients with depressive and anxiety disorders" ppt
... drop-out of treatment Treatment drop-out is defined as: the patient is in need of more therapy in the therapist’s opinion but ignores at least two invitations of the therapist and does not continue the ... In both groups disciplines of the therapists, the average amount of years of general treatment experience and ethnic background are evenly distributed Intervention Therapists of the intervention ... assistant before the intake procedure starts Then the patients are given an appointment for an intake Page of interview by a therapist from the matching condition After this intake interview the...
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báo cáo khoa học: " Management of venous thrombosis in fibular free osseomusculocutaneous flaps used for mandibular reconstruction: clinical techniques and treatment considerations" pdf
... necrosis of the lower lip and radiofibrosis of the surrounding soft tissue In June 2008, the reconstruction plate perforated the epidermis and was subsequently removed Two years after the first ... coagulation of the punctual bleeding The flap showed recovery of the venous function hours after revision surgery detectable by the fading of the dissemi- Page of Figure Fibular flap harvested from the ... informed consent was obtained from the patient for publication of this case report and accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this...
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Another Piece of the Action
... that exact same moment of time There was the smell of the perfume she had chosen that day, the quality of her voice, the people that were in the corridor at the same time, and the way she had looked ... bothered to the math Until that is, a second Earth was discovered Miri’s planet, named by Captain Kirk for the young lady he had the fortune of meeting, was found to be indistinguishable from the ... hadn’t discovered it, but he was there.) Forget for a moment that both planets had sought the development of a biological means for prolonging human life And forget for the moment that these cultures...
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iec 60079-15 electrical apparatus for explosive gas atmospheres - construction, test and marking of type of protection n electrical apparatus
... is indispensable for the correct application of this publication 9) Attention is drawn to the possibility that some of the elements of this IEC Publication may be the subject of patent rights ... which the contacting mechanism is constructed so that the component is not capable of causing ignition of the specified explosive gas atmosphere NOTE The enclosure of the non-incendive component is ... referenced documents are indispensable for the application of this document For dated references, only the edition cited applies For undated references, the latest edition of the referenced document...
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Tài liệu Activity 1.1: Designing a New Type of Motorcycle doc
... New Type of Motorcycle Exercise 1: Determining Your Gathering and Analysis Process Your research indicates that more people would like to ride motorcycles, but that they not like the design of ... different types of questions Assume that the three sources you identified have provided all of the information you need to start your motorcycle project List four steps you can take to analyze the information ... motorcycles You need to collect information to make an informed decision about the type of motorcycle that will attract new riders After you collect and analyze the information, you will be able to...
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A study of using english songs as a type of supplementary material in teaching listening for first year non major students of english at phuong dong university
... content of the songs, this task is really helpful as it enables them to have a full understanding of the song It is quite unexpected that dictation is at the bottom of the list Of 19.9% of the students ... things that prevent the songs from developing their listening skill as follows: 21 The first thing is the music of the songs This seems quite absurd but it is the music of the song that distracted ... process We can see that listening is active because there is often a great difference between what is said and what the listener “hears” It is obvious that the listener is constructing his or own meaning,...
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Tài liệu Báo cáo khoa học: A novel type of highly negatively charged lipooligosaccharide from Pseudomonas stutzeri OX1 possessing two 4,6-O-(1-carboxy)-ethylidene residues in the outer core region ppt
... whereas little is known about LPS alterations On the basis of this novel structure, we can advance the hypothesis that the presence of pyruvate residues in lipopolysaccharides is a new, structurally ... sugars It is worth noting that the core oligosaccharide of LOSs has been reported to play a role in the interaction of the microorganism with the environment [12,13] In this paper, the structural ... core regions Based on this finding, the hypothesis can be advanced that the insertion of pyruvate residues at the end of the oligosaccharide chain blocks its elongation, thereby leading to a shorter...
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Đề tài " The homotopy type of the matroid grassmannian " ppt
... is an analysis of the homotopy type of the classifying space of the accompanying bundle theory Secondly, it might point the direction for possible further results concerning the application of ... phenomena of smooth manifolds, that is, whether it can see more than the PL structure The aim of this article is to prove the following theorem Theorem 1.1 For every positive integer n or for n = ... and for any k ≤ n, the map π : G(k, n) → MacP(k, n) is a homotopy equivalence Of course, in the case n = ∞, this result implies that the theory of matroid bundles is the same as the theory of...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25