adipose stem cell engineering characterization and current application in neurological tissue repair
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... tool for tissue engineering, cellular transplant therapy and as drug discovery tools [2, 3, 4, 7, 13, 14] In tissue engineering, stem cells can possibly be used to generate healthy tissue to ... problem in drug discovery Despite the promise of hESCs in tissue engineering and cellular transplant therapy, the gap between scientific research and clinical applications for safe and effective stem ... would in nature, and in similar proportions to that of in vivo ECM is formed from many different structural macromolecules and plays a crucial role in maintaining tissue structure and modulating cell...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, based on work by Hughes et al 2009 † Murine protein is a homolog of human protein 50 ... metalloproteinase with thrombospondin motifs 1*† Transforming growth factor-beta-induced protein ig-h3 74665 122841 234698 Isoform of Extended synaptotagmin-1*† Isoform of Myoferlin*† 99.80% ... 105340 Keratin, type I cytoskeletal 10 Keratin, type II cytoskeletal Collagen alpha-1(I) chain*† Collagen alpha-2(I) chain*† EMILIN-1*† Fibrillin-1* Isoform of Collagen alpha-1(XII) chain Isoform...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... contains more Fn (by immunofluoresence comparison, section 3.3.2), it would be expected that the 52 increased presence of Fn would aid in increasing integrin binding and hence cell binding This ... due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs, since DCN and BGN both bind active TGFB, and could lead ... amounts of proteins within and their complexity (refer to section 3.3), not allow the adherence of hESCs and also not maintain hESCs in a pluripotent state This observation is surprising, since these...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
... of hESCs, and so, in order to obtain successful Oct-4 immunostaining, the cells have to be first permeabilized using 0.2% Triton-X 100 - this extra processing step results in markedly increased ... trophoectoderm lineages [10], indicating the crucial role that Oct-4 plays in maintaining the pluripotent state of ES cells As Oct-4 is a transcription factor, naturally it is found within the nuclei ... down-regulated in trophoectoderm and primitive endoderm of the mouse embryo Later, it is maintained in the embryonic ectoderm at the cylinder stage, and down-regulated at gastrulation in an anterior-posterior...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
... at increasing the population doublings and also in 84 maintaining the pluripotent characteristics of the hESCs during their propagation Once again, hESCs were cultured onto Matrigel, DxSDOC and ... Stem Cell Bank In this protocol, the hESCs formed embryoid bodies using non-adherent cell culture plates and were transferred onto laminincoated plates and incubated in neural induction medium ... In vitro induced differentiation hESCs cultured on DxSDOCDOC for 13 passages were induced into neural differentiation using a protocol modified from WiCell Research Institute, National Stem Cell...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
... current cell culture methods employed during hESC culture To attain the goal of clinical application in tissue engineering, stem cell transplant therapy and also drug discovery, hESCs have to be maintained ... Kaukinen K, Maki M, Lindfors K Extracellular transglutaminase has a role in cell adhesion, whereas intracellular transglutaminase is involved in regulation of endothelial cell proliferation and ... Raghunath M Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell- based therapies Adv Drug Deliv Rev, in press (2011)...
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Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) potx
... B - cell differentiation from a human pancreatic cell line in vitro and in vivo Mol Endocrinol 15, 476 - 483 Dzierzak, E et al (1998); Embryonic beginnings of definitive hematopoietic stem cells ... 88 - 95 Itkin - ansari, P., et al (2001); PDX - and cell - cell contact act in synergy to promote d - cell development in a human pancreatic endocrine precursor cell line Mol Endocrinol 14, 814 ... circulating human embryonic blood stem cells Blood 96, 1740 - 1747 IItskovitz - Eldor, J and et al (2000).; Differentiation of human embryonic stem cells into embryoid bodies comprising the three...
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Báo cáo y học: "Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis" doc
... LIF/Stat3 pathway for murine ES cells [3], and bone morphogenetic protein [4] and the Wnt pathway [5] for both mouse and human stem cells Intrinsic factors that maintain selfrenewal include the transactivators ... released4ES cellsSox17, Brac and Mash1 during EB formation of control and mRNA mRNA levels of Sox17, Brac and Mash1 during EB formation of control and released ES cells (ES re) Control and released ES cells ... modifications in TSA-treated ES cells and EBs Analysis of bulk histone modifications in TSA-treated ES cells and EBs Global levels of histone H3 acetylation (acH3), and lysine (3mK4) and lysine 27 (3mK27)...
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Exploring mesenchymal stem cell derived exosome and tocotrienol (t3) as therapeutic agents in drug induced liver injury (DILI)
... analogs on APAP and H2O2-induced on cell death in TAMH cells 112 4.3.2 Effect of lower dosage of -T3 and -T3 against APAP and H2O2-induced injury on cell viability in TAMH cells 118 4.3.3 ... effective in preventing both toxicants induced injuries -T3 preserved cell viability by acting against the build-up of ROS and its downstream pathway which inhibited the injury and initiation ... of -TP, -T3 in cell viability after APAPand H2O2-induced injury in TAMH hepatocytes 120 Figure 32 Effects of -TP, -T3 in cell viability after APAP- and H2O2induced injury in TAMH hepatocytes...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 10
... have interactions with both injured tissues and transplanted stem cells, which may affect their behaviors Moreover, in in vivo the blood circulation and joint motion may have some role in stem cell ... Buschmann MD Fibronectin, vitronectin, and collagen I induce chemotaxis and haptotaxis of human and rabbit mesenchymal stem cells in a standardized transmembrane assay Stem Cells Dev 2007 Jun;16(3):489-502 ... mesenchymal stem cell implantation of femoral condyle Immunohistochemistry (IHC) staining against collagen I (A); IHC against collagen II (B); IHC against collagen X (C); and Alcian blue staining against...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 1
... 1.11 Cellular MRI MRI is a practical imaging tool used to track administered cells in studies for tissue engineering, cellular transplantation, and homing and migration of cells In tissue engineering, ... reprogramming of the adult cells without destroying embryos at early stages 15 1.5.3 Adult stem cells Adult stem cells, or tissue- specific stem cells, are isolated from different adult tissues and ... Hepatic stem cells In or near the termnal bile ductules (canals of Hering) Oval cells that subsequently generate hepatocytes and ductular cells Pancreatic stem cells Intraislet, nestin-positive cells,...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 2
... determined using 0.3% oil red O stain for 15 minutes at room temperature to stain intracellular lipids, and counterstained with hematoxylin Osteogenic differentiation was evaluated using Alizarin ... immunohistochemical staining, sections of labeled and unlabeled pellets were fixed on slides, and were pre-digested with pepsin (1 mg/ml in Tris–HCl, pH 2.0) for 30 minutes at room temperature, and incubated ... biotinylated goat anti-mouse for 30 minutes and after washing samples were incubated with streptavidin peroxidase for 45 minutes at room temperature Sections were visualized using DAB chromogen and...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 3
... Alizarin red staining showed that MSCs labeled with SPIO did not affect osteogenic differentiation capacity of cells (figure 3.10) Figure 3-10 Alizarin Red staining Alizarin Red staining of ... Immunohistochemical staining after high density pellet culturing showed that the distribution of collagen type II in extracellular matrix of cells was same between unlabeled and 25 and 50 µg/mL SPIO labeling, ... exhibited no difference in developing fat vacuoles (Figure 3.9) 64 Figure 3-9 Oil red O staining Oil red O staining of the MSCs after 21 days adipogenic differentiation induction; Unlabeled...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 4
... process and the secretion of proinflammation factors associated with remodeling and repair of the injury provided the stimuli for MSC migration MRI revealed that the injected cells were in defect ... site, para-patellar fat and injection site of the knee joint (figure 3.15 and 3.16) 71 Figure 3-15 MR imaging of the mini-pig's femoral condyle MR imaging of the mini-pig’s femoral condyle ... mini-pig knee joint The labeled cells migrated to the para-patellar fat tissue (A) and surgical scar in the skin (B) 3.4.8 Postmortem analysis Histological grading scores of the repaired cartilage...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 6
... cells can be a promising tool for real time evaluation and tracking of MSCs, providing data for a better understanding of the migration and localization of the injected cells 76 ... and injection site, confirming the report of Jing et al (206, 207) In addition, our results showed that we could detect labeled cells in vivo up to weeks, thus the SPIO labeling method of the cells ... cells negatively affected the osteogenic differentiation of the cells in vivo In contrast, our study showed that iron labeling did not affect the adipogenic and osteogenic potential of MSCs in...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 7
... simultaneous culturing and monitoring of uninjured cartilage tissue, injured cartilage tissue and MSCs Then, migration of MSCs against the injured cartilage tissue was evaluated Exploring the interaction ... of the cells Uninjured and injured cartilage tissues were prepared by using an established method Conditioned media were prepared by culturing uninjured and injured cartilage tissues in complete ... MSCs for cartilage repair relies on the homing and integration of the cells to the injured tissue Although there are speculations that other injured tissues express ligands and chemotactic factors...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... active in ES cells and that mature Lefty2 proteins are secreted into and deposited in the ES cell microenvironment 79 2.1.2.8 LEFTY2 maintains ES cell self renewal by inhibiting Nodal signaling ... Supplementing Lefty2 RNAi cultures with recombinant Lefty1 protein was able to block reduction in level of alkaline phosphatase staining induced by Lefty2 RNAi Alkalline phosphatase staining was ... differentiate the ES cells The cells were then dissociated with trypsin and replated at single cell density to allow the ES cells to expand into colonies It was interesting to note that the number...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
... signals dictate embryonic stem cell fates is also not entirely clear Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation have ... manner in vitro is addressed Interestingly, it was found that Nodal signaling is able to arbitrate at least three distinct fate decisions in ES cells Perturbation of endogenous signaling in ES cells ... elicit effects in embryos in a dose dependent manner In this thesis, the possibility that Nodal signaling might, as in the in vivo scenario, likewise influence ES cell fate decision in a dose dependent...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... were maintained on uncoated cell culture dishes in ES cell medium containing DMEM/20% ES FBS (Invitrogen, Carlsbad, CA), 0.1 mM MEM nonessential amino acids (Invitrogen), 2mM L-glutamine (Invitrogen), ... days growth in selective medium, few cells remained in the untransfected control wells indicating that selection was effective The surviving cells were trypsinized and resuspended in ES cell medium ... Protein extraction and western blotting To obtain protein extracts, cells were trypsinized from culture dishes harvested in chilled PBS, centrifuged at 10,000g for at °C, washed again in PBS and incubated...
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