adaptation of human pluripotent stem cells to feeder free conditions in chemically defined medium with enzymatic single cell passaging
Atlas of Human Pluripotent Stem Cells doc
... apply to the field of pluripotent stem cells Induced pluripotent stem cells attract great interest for their potential in understanding the basics of cell reprogramming, personalized medicine and ... recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for studying the fundamentals of early human development ... antibodies into the blastocyst, thus leaving the ICM cells intact Incubation with guinea pig complement-containing medium then lyses all antibodymarked cells The intact ICM is further rinsed and...
- 157
- 579
- 0
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... histone variant MacroH2A; meC, methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including ... regulatory elements CTCF, CCCTC-binding factor, insulator associated protein; ESC, embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone ... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone...
- 13
- 338
- 0
Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell phenotypes upon interaction ... 1987;Integrins: a family of cell surface receptors Cell (4):549-554 18 Damsky CH, Werb Z 1992;Signal transduction by integrin receptors for extracellular matrix: cooperative processing of extracellular...
- 9
- 423
- 0
Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... xeno -free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial ... temperature Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells were washed off prior to the MTT assay From ... a feeder layer of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc...
- 6
- 477
- 0
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the ... been reported to be highly expressed in hematopoietic stem cells (HSCs) from human umbilical cord blood, and it promotes differentiation of HSCs into progenitor cells by inhibiting ABCG2 expression[56] ... the samples into three groups: hES cells, EB, and adult cells Node A contained the genes that were upregulated in both hES cells and EB, node B contained the genes upregulated in hES cells only,...
- 17
- 593
- 0
Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... cells/ cm2, trypsinised and stored in Recovery Cell Culture Freezing Medium in liquid nitrogen Before osteogenic differentiation, 6,000 cells/ cm2 of human MSCs were seeded in BM in each well of ... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... in murine cell lines and accelerates in vivo bone formation in an intra-membranous animal model [25] Whether noggin is effective in human cells undergoing osteogenic differentiation remains to...
- 9
- 404
- 0
Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... Vol 11 No Steinert et al Introduction Mesenchymal progenitor cells, also referred to as mesenchymal stem cells (MSCs), provide an attractive alternative to chondrocytes with regard to cell- based ... resulted in sustained regeneration of hyaline cartilage in vivo [7] Inadequate delivery of the soluble factors necessary to drive the chondrogenic differentiation of the transplanted cells in vivo ... minutes, resuspended in complete DMEM (containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin), and plated at to × 107 nucleated cells per 75 cm2 flask (Falcon, Beckton Dickinson...
- 15
- 830
- 0
Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... Immunohistochemistry (IHC) in stage IV cells further revealed adding ECM increased the percentage of proinsulin and insulin expressing cells with the maximum effect seen in cells treated with LAM (Figure ... IV cells (A) Immunohistochemistry shows the expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, ... Harrison LC: Distinct distribution of laminin and its integrin receptors in the pancreas J Histochem Cytochem 2002, 50(12):1625-1632 Hulinsky I, Harrington J, Cooney S, Silink M: Insulin secretion...
- 10
- 332
- 0
Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... (TRITC)-conjugated phalloidin (staining red F-actin) and DAPI (staining blue nuclei) The staining showed the formation of actin cytoskeleton in a 3D architecture for the cells cultured on the 3D ... incorporation of Dex an osteoinductive drug into polymeric nanofibers during electrospinning process 1) Incorporation of Type I Col into nanofibrous scaffolds can improve nanoHA mineralization on the nanofibers ... monocytic cells; mineralization inhibitor Glycoproteins Osteonectin Expressed in a variety of connective tissues; strong affinity for Ca2+; may mediate deposition of HA; binds to growth factors; may influence...
- 272
- 258
- 0
Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), ... Histone lysine 27 trimethylation HRE Hormone response element ICM Inner cell mass iPSC Induced pluripotent stem cell kb Kilo basepairs LBD Ligand binding domain LIF Leukemia inhibitory factor LincRNA ... Nanog-null embryos fail to form a proper epiblast and cells within the ICM tend to differentiate into parietal endodermal-like cells2 5 In addition, cells devoid of Nanog tend towards extraembryonic...
- 138
- 342
- 0
Baculovirus mediated genetic modification of human embryonic stem cells
... properties to combat specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts ... is of great importance in making use of the remarkable potential of ES cells Possible benefits of genetic manipulation include controlling differentiation of ES cells, screening and acquiring ... pure populations of specific types of ES cell- derived cells, altering antigenicity of cells to overcome immune rejection in transplantation medicine, and providing cell sources with new functional...
- 135
- 218
- 0
The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their ... delivery of stem cells to the tissues in which they are needed Current practice involves either the injection of stem cells directly into the targeted tissue, or injection of the stem cells into the ... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells...
- 207
- 366
- 0
Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... differentiate in presence of cytokines to generate haematopoietic colonies Figure 3.6 hESCs cells grown on Ortec143 differentiated in presence of cytokines to give rise to haematopoietic-like cells Figure ... hESCs maintained on human feeder cells are amenable to haematopoietic differentiation 80 3.3.1 hESCs maintained on CCD919 cells 82 3.3.2 hESCs maintained on Ortec143 cells ... mechanism in part can be attributed to the differentiation of the mixed pool of donor haematopoietic cells into allogeneic antigen-presenting cells (APCs) DCs are potent antigen presenting cells of...
- 296
- 2,020
- 0
IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... time-point: (a) (c) nuclear staining with DAPI (b) Immunostaining with bone specific ALP (arrow showing the cells positive for ALP stain) (d) Immunostaining with osteonectin (arrow showing cells ... marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells Adult stem cells can be derived ... Seeding of cells into scaffolds 12 1.3.4 Cell source for cell- based tissue engineering for bone regeneration 12 1.4 Stem cells in bone repair 13 1.5 Human Embryonic stem...
- 77
- 972
- 0
Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem cells 1.1.4 Human ... loops to maintain the pluripotency in human ES cells Page Figure Regulatory networks and transcription factors in maintenance of human ES cells bFGF is an essential component in human ES cell ... pluripotency in human ES cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It...
- 109
- 371
- 0
Sensibility study of flooding and drying issues to the operating conditions in PEM fuel cells
... channel and the GDLs as in well as the channel inlet conditions ( SR in , RH in , Ptot , y in ) which give Qiin and yiin ISSN 2076-2895 (Print), ISSN 2076-2909 (Online) ©2010 International Energy ... the cell Thus, the two next sections (sections 3.1.2 and 3.1.3) focus on finding a pertinent location allowing to interpret the appearance of liquid water in terms of an ideal operating point ... performances According to this observation, it is needed to predict the influences of the operating conditions on the internal cell humidification as well as on the cell performances In this way, the...
- 20
- 571
- 0
báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of ... purposes) Journal of Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected ... VE-cadherin (-) / TRA-1 (-) cells expansion with VEGF expansion with PDGF - BB VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells hES -MCs Figure cells differentiated...
- 14
- 450
- 0
Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes but not ... cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... chromatin-opening activity In other words, HS3 could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3...
- 10
- 422
- 0
báo cáo hóa học:" Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor" docx
... points after cell injection We wanted to evaluate differential kinetics of cells retention in the lungs as a measure of cell- cell interaction achieved by MSC-AR in vivo (Fig 3B-E) Since the kinetics ... using the Adobe Photoshop 7.0 software Statistics on bioluminescent signal intensity was obtained using WinView software according to the software instruction For comparison of tumor targeting ... erbB2 expressing cells MSC-AR bind to erbB2-expressing cells in vivo Differential kinetics of MSC lung clearing in a transient transgenic model We next investigated if membrane expression of the AR...
- 14
- 339
- 0
Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps
... P63+ basal /stem cells were transformed into AR+ or ARcancer cells but rarely into ChgA+ neuroendocrine cancer cells Conventional human prostate cancer cells include malignant cells of luminal, basal ... the P63+ basal /stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells in regions of primary PIN lesions (Figure 3B; arrow in (b) indicated P63 ... arrowheadindicated in (a)) and hyperplasic basal cells (Figure 3A; arrow-indicated in (b)), P63 was detected in the cytoplasm of cells in the HGPIN (high grade PIN) lesions (Figure 3A; arrow-indicated in (c))...
- 11
- 446
- 0
- morphology of human embryonic stem cells and induced pluripotent stem cells cultured in feeder layer free conditions
- method for the derivation of induced pluripotent stem cells from human hair follicle keratinocytes
- array comparative genomic hybridization characterization of human pluripotent stem cells
- analysis of the methylome of human embryonic stem cells employing methylated dna immunoprecipitation coupled to next generation sequencing
- xeno free culture of human pluripotent stem cells
- culture of human pluripotent stem cells on glass slides for high resolution imaging
- fish analysis of human pluripotent stem cells
- immunocytochemical analysis of human pluripotent stem cells
- flow cytometric analysis of human pluripotent stem cells
- genome wide epigenetic analysis of human pluripotent stem cells by chip and chip seq
- human pluripotent stem cells the development of high content screening strategies
- quantitative proteome and phosphoproteome analysis of human pluripotent stem cells
- converting human pluripotent stem cells to neural tissue and neurons to model neurodegeneration
- differentiation of human pluripotent stem cells into retinal cells
- isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008