xeno free culture of human pluripotent stem cells
Atlas of Human Pluripotent Stem Cells doc
Ngày tải lên :
22/03/2014, 09:20
... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... Feeder Culture Methods 2.3.2 hPSC Culture References Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder Layer -Free Conditions...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
Ngày tải lên :
11/08/2014, 12:21
... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3;...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... efficient and safe xeno- free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... and m-FISH (Figure 3) of metaphase spreads Hence, the results of this study could have useful implications for the salvage of hESC culture during infrequent occurrences of incubator break-down ... small-scale culture for research or large-scale culture within bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
Ngày tải lên :
09/08/2014, 10:23
... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... cells/ cm2, trypsinised and stored in Recovery Cell Culture Freezing Medium in liquid nitrogen Before osteogenic differentiation, 6,000 cells/ cm2 of human MSCs were seeded in BM in each well of ... chemicals used were from Sigma-Aldrich (St Louis, MO, USA), unless stated otherwise Culture of human mesenchymal stem cells Human MSCs were obtained from two sources, the first one being a commercially...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
Ngày tải lên :
09/08/2014, 14:22
... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae ... (approximately 1.2 × 106 cells/ T-75 flask), the cultures were infected in 750 μL serumfree DMEM for two hours at a dose of × 103 vp/cell of Ad.BMP-2, or Ad.BMP-4 Control cultures were similarly...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
Ngày tải lên :
10/08/2014, 05:21
... Differentiation of human embryonic stem cells into insulin-producing clusters Stem Cells 2004, 22(3):265-274 Chang CF, Hsu KH, Chiou SH, Ho LL, Fu YS, Hung SC: Fibronectin and pellet suspension culture ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells ... and survival of adherent cells [17] FN and LAM constitute the major ECM of pancreas islet cells Laminin-1 has been reported to promote the differentiation of fetal mouse pancreatic b -cells [18]...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
Ngày tải lên :
09/09/2015, 10:07
... images of bone nodules (indicated by arrows) produced by human MSCs of passage on the PLLA nanofibers and TCPS plates (control) in the Ost medium after 21 days of culture The cells on the nanofibrous ... days of culture with human MSCs The calcium content was normalized by the number of viable cells Significant difference of investigated groups was denoted as * (p < 0.05) The cells were also cultured ... Table 2.9 Classification of stem cells [23-30] 51 Table 2.10 Advantages and disadvantages of fabrication methods of nanofibers 79 Table 2.11 Clinical trials using nanofibers 88 Table...
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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
Ngày tải lên :
09/09/2015, 18:56
... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
Ngày tải lên :
11/09/2015, 09:16
... differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal ... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, ... and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive niches...
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Baculovirus mediated genetic modification of human embryonic stem cells
Ngày tải lên :
11/09/2015, 14:38
... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF ... BACULOVIRAL TRANSDUCTION OF HUMAN EMBRYONIC STEM CELLS 11 2.1 Introduction hES cells hold great potential as a system for the study of lineage specification and as an unlimited source of cells for the regenerative...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
Ngày tải lên :
16/09/2015, 15:55
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of pluripotent ... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells ... the in vitro manipulation of stem cells 35 CHAPTER Derivation and Propagation of Human Embryonic Stem Cell Lines on Xeno- Free Support Systems and in the Presence of Xeno- Free Proteins 36 Introduction...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
Ngày tải lên :
04/10/2015, 16:03
... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic stem cells ... analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of ... in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
Ngày tải lên :
09/10/2015, 11:24
... 1.4 Stem cells in bone repair 13 1.5 Human Embryonic stem cells 15 1.5.1 Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells ... experiments Culture of H1 cells in Matrigel For in vivo experiments H1 cells cultured in feeder -free culture system were used Confluent H1 cells grown on feeder layer were removed from the culture ... specific differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
Ngày tải lên :
16/10/2015, 11:58
... embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem cells 1.1.4 Human ... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... studies of LIN28 19 20 Objective of the current study Page II Materials and methods 22 2.1 Culture of human ES cell line 22 2.1.1 Preparation of feeder cells 22 2.1.2 Maintenance of human ES cells...
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Báo cáo y học: " Induced pluripotent stem cells free of exogenous reprogramming factors" pot
Ngày tải lên :
14/08/2014, 21:20
... approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells from mouse embryonic ... Tian S, Stewart R, Slukvin, II, Thomson JA: Human induced pluripotent stem cells free of vector and transgene sequences Science 2009, [Epub ahead of print] Aasen T, Raya A, Barrero MJ, Garreta ... Induced pluripotent stem cell lines derived from human somatic cells Science 2007, 318:1917-1920 Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S: Generation of mouse induced pluripotent stem...
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Establishment of autologous culture systems for human embryonic stem cells
Ngày tải lên :
11/09/2015, 10:00
... Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff Bongso, ... MatrigelTM-mTeSRTM1 system vii Table of Contents 143 5.4 Supportive effect of the xeno- free, feeder -free and serum -free system for the growth of hESCs 145 5.4.1 Establishment of autologous...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
Ngày tải lên :
18/06/2014, 15:20
... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted Schematic representation of preparation of the transplanted vascular cells differentiated ... examination of cultured cells Staining of cultured cells on dishes at 5th passage was performed as described elsewhere [8,10] Monoclonal antibodies for alpha smooth muscle actin (αSMA) (Sigma), human...
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báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx
Ngày tải lên :
11/08/2014, 12:21
... programs of human embryonic and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human- induced pluripotent ... Genetic engineering of human pluripotent cells using TALE nucleases Nat Biotechnol 2011, 29:731-734 88 Zhao T, Zhang ZN, Rong Z, Xu Y: Immunogenicity of induced pluripotent stem cells Nature 2011, ... retention of human- induced pluripotent stem cells PLoS One 2009, 4:e7076 Ghosh Z, Wilson KD, Wu Y, Hu S, Quertermous T, Wu JC: Persistent donor cell gene expression among human induced pluripotent stem...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
Ngày tải lên :
09/09/2015, 10:16
... Chan, SK Chong, Z Zhang, MA Choolani, SH Teoh Coculture of human endothelial progenitor stem cells and bone marrow-derived human fetal mesenchymal stem cells potentiates osteogenesis through paracrine ... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells ... Analysis 87 Chapter – Coculture of Human Umbilical Cord Blood Endothelial Colony-Forming Cells with Human Fetal Mesenchymal Stem Cells for the Generation of Vascularised BTE Grafts ...
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